Selection of soybean mutant lines with altered seed coat colour and their antioxidant activity

Seed coat colour is an important determinant of the visual quality of dry beans, as seeds are sold as a dry commodity. Phenolic compounds have a major effect on the colour of bean seeds. The objectives of the study were to determine the changes in phenolic compounds during seed development and in whole seeds of yellow bean genotypes with contrasting seed coat colour, and the effects of storage temperature and duration on seed phenolics and colour. Condensed tannin, phenolic acid, flavonoids, and antioxidant activity were observed as early as 10 d after flowering in the developing seeds of Arikara Yellow, which darken at harvest and during postharvest storage. In contrast, for CDC Sol and AAC Y073 seeds which remain yellow, phenolic compounds and antioxidant activity were consistently low. Seed brightness (L*) and yellow colour (b*) were negatively correlated with phenolic compounds and antioxidant activity, and conversely seed redness (a*) was positively correlated with phenolic compounds, confirming a negative influence of phenolic compounds on seed coat colour. Yellow bean genotypes had low anthocyanin but were high in β-carotene. Storage temperature influenced condensed tannin and seed coat colour, whereas the duration of storage influenced phenolic compounds, antioxidant activity, and seed coat colour. Higher temperatures (20 or 30 °C) and longer storage duration (120 or 180 d) generally resulted in darker seeds with increasing redness compared with seeds stored at 6 °C or for 60 d. AAC Y073 and CDC Sol with improved seed coat colour may increase consumer preference, value, and marketability of yellow beans.

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Phenolic Constituents, Ascorbic Acid, Tocopherols and Antioxidant Activity of Soybean Genotypes with Varying Seed Coat Colour

Agricultural Research Journal ◽

10.5958/2395-146x.2016.00072.7 ◽

2016 ◽

Vol 53 (3) ◽

pp. 376

Author(s):

Heena Miglani ◽

Sucheta Sharma

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Seed Coat ◽

Coat Colour ◽

Seed Coat Colour ◽

Phenolic Constituents ◽

Soybean Genotypes

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Antioxidant activity of Phaseolus L. species seeds with different coat colour

Žemės ūkio mokslai ◽

10.6001/zemesukiomokslai.v25i3.3794 ◽

2018 ◽

Vol 25 (3) ◽

Author(s):

O. N. Bezuhla ◽

V. V. Pozdniakov ◽

L. N. Kobyzeva ◽

R. L. Boguslavskiy ◽

O. V. Antsyferova

Keyword(s):

Antioxidant Activity ◽

Chlorogenic Acid ◽

Seed Coat ◽

Plant Genetic Resources ◽

Coat Colour ◽

Seed Coat Colour ◽

High Antioxidant Activity ◽

Olive Seeds ◽

Mature Seeds

The aim of the research was to screen the antioxidant activity of mature seeds of related bean species using the collection samples of the National Center for Plant Genetic Resources of Ukraine: Phaseolus vulgaris L., P. multiflorus Lam., P. lunatus L. and P. acutifolius A. Grey which have different colouration of their seed shell. The determination of antioxidant activity was carried out using the improved method of the stable radical DPPH•: methyl alcohol in the eluting solution was replaced by non-toxic ethyl alcohol. The results of the analysis are presented as the equivalent of a standard antioxidant (chlorogenic acid), expressed in mg of the standard per 1 g of a sample, taking into account dilutions according to the calibration schedule. As a result of the Phaseolus L. collection screening, it was found that the antioxidant activity of bean seeds depended on the colour of the seed coat. Regardless of the bean type, white seeds had the lowest indicator (equivalent to chlorogenic acid is 0.82–0.96 mg g–1 of seed). Painted seeds have different antioxidant activity. In P. vulgaris L., olive seeds have the highest index (4.21–5.07 mg g–1 of seeds), it is 2.36–3.60 mg g–1 in wine red seeds and 2.72–3.21 mg g–1 in pink ones. And the tendency of the influence of drawing colour and the area of a seed coat is noticed. In P. lunatus L., the antioxidant activity of white seeds with a motley wine-red pattern (2.27 mg g–1 of seeds) is more than twice as high as of white seeds without a pattern (0.94 mg g–1 of seeds). In P. multiflorus Lam., all the coloured seeds had a very high antioxidant activity regardless of the seed coat colour and its pattern (6.09–6.16 mg g–1 of seeds). In P. acutifolius A. Gray, the change in this parameter, depending on the seed coat colour, was small (1.27–1.62 mg g–1 of seeds). It was found that the antioxidant activity of beans depended on the environmental conditions: the stress of plants because drought leads to its increase.

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Identification of candidate genes for the seed coat colour change in a Brachypodium distachyon mutant induced by gamma radiation using whole-genome re-sequencing

Genome ◽

10.1139/gen-2016-0145 ◽

2017 ◽

Vol 60 (7) ◽

pp. 581-587 ◽

Cited By ~ 3

Author(s):

Man Bo Lee ◽

Dae Yeon Kim ◽

Yong Weon Seo

Keyword(s):

Seed Coat ◽

Brachypodium Distachyon ◽

Colour Change ◽

Coat Colour ◽

Dna Polymorphisms ◽

Cereal Crops ◽

Whole Genome ◽

Seed Colour ◽

Seed Coat Colour ◽

Mutant Lines

Brachypodium distachyon has been proposed as a model plant for agriculturally important cereal crops such as wheat and barley. Seed coat colour change from brown–red to yellow was observed in a mutant line (142-3) of B. distachyon, which was induced by chronic gamma radiation. In addition, dwarf phenotypes were observed in each of the lines 142-3, 421-2, and 1376-1. To identify causal mutations for the seed coat colour change, the three mutant lines and the wild type were subjected to whole-genome re-sequencing. After removing natural variations, 906, 1057, and 978 DNA polymorphisms were detected in 142-3, 421-2, and 1376-1, respectively. A total of 13 high-risk DNA polymorphisms were identified in mutant 142-3. Based on a comparison with DNA polymorphisms in 421-2 and 1376-1, candidate causal mutations for the seed coat colour change in 142-3 were selected. In the two independent Arabidopsis thaliana lines carrying T-DNA insertions in the AtCHI, seed colour change was observed. We propose a frameshift mutation in BdCHI1 as a causal mutation responsible for seed colour change in 142-3. The DNA polymorphism information for these mutant lines can be utilized for functional genomics in B. distachyon and cereal crops.

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Correlation Studies of Some Agronomic Characters in Sesame

Experimental Agriculture ◽

10.1017/s0014479700005895 ◽

1970 ◽

Vol 6 (1) ◽

pp. 27-31 ◽

Cited By ~ 7

Author(s):

M. Osman Khidir ◽

H. El Gizouli Osman

Keyword(s):

Seed Size ◽

Seed Coat ◽

Coat Colour ◽

Stem Height ◽

Agronomic Characters ◽

Seed Coat Colour ◽

Correlation Studies ◽

First Maturity ◽

Criteria For Selection ◽

Number Of Branches

SummaryIn 90 local sesame types there was some association between seed coat colour and seed size, stem height, number of branches, number of pods, yield per plant and earliness. Forty-five coefficients show the degree of correlation between ten agronomic characters. Yield was significantly and positively correlated with all characters except the number of days to first flowering and to first maturity. Stem height, number of pods per plant and seed size seem to be the best criteria for selection in sesame.

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