Gene cloning of ZmMYB59 transcription factor in maize and its expression during seed germination in response to deep-sowing and exogenous hormones

2017 ◽  
Vol 136 (6) ◽  
pp. 834-844 ◽  
Author(s):  
Longgang Du ◽  
Hongye Jiang ◽  
Guangwu Zhao ◽  
Jingyu Ren
Keyword(s):  
Transcription Factor ◽  
Seed Germination ◽  
Gene Cloning ◽  
Exogenous Hormones

Gene cloning and analysis of the pattern of expression of the transcription factor HymMYB2 related to blue flower formation in Hydrangea macrophylla

Euphytica ◽  
2021 ◽  
Vol 217 (6) ◽  
Author(s):  
Jiqing Peng ◽  
Chao Xue ◽  
Xujie Dong ◽  
Chaozhen Zeng ◽  
Yi Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Cloning ◽  
Blue Flower ◽  
Flower Formation ◽  
Hydrangea Macrophylla

A seed-specific AP2-domain transcription factor from soybean plays a certain role in regulation of seed germination

2008 ◽  
Vol 51 (4) ◽  
pp. 336-345 ◽  
Author(s):  
ChunMei Wang ◽  
HuiWen Wang ◽  
JinSong Zhang ◽  
ShouYi Chen
Keyword(s):  
Transcription Factor ◽  
Seed Germination ◽  
Ap2 Domain

scholarly journals Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

2020 ◽  
Author(s):  
Kaihui Zhai ◽  
Guangwu Zhao ◽  
Hongye Jiang ◽  
Caixia Sun ◽  
Jingyu Ren
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Seed Germination ◽  
Transgenic Tobacco ◽  
Germination Rate ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Myb Transcription Factors ◽  
Vigor Index

Abstract Background MYB transcription factors are involved in many biological processes, including metabolism, development and responses to biotic and abiotic stresses. In our previous work, a new MYB transcription factor gene, ZmMYB59 was induced by deep sowing and down-regulated during maize seed germination via Real-Time PCR. However, there are few reports on seed germination regulated by MYB proteins and the functions of ZmMYB59 remain unknown. Results In this study, to examine its functions, Agrobacterium -mediated transformation was exploited to generate ZmMYB59 transgenic tobacco and rice. In T 2 generation transgenic tobacco, germination rate, germination index, vigor index and hypocotyl length were significantly decreased by 25.0~50.9%, 34.5~54.4%, 57.5~88.3% and 21.9~31.2% compared to wild-type (WT) lines. In T 2 generation transgenic rice, germination rate, germination index, vigor index and mesocotyl length were notably reduced by 39.1~53.8%, 51.4~71.4%, 52.5~74.0% and 28.3~41.5%, respectively. On this basis, relative physiological indicators were determined. The activities of catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and proline content of transgenic lines were significantly lower than those of WT, suggesting that ZmMYB59 reduced their antioxidant capacity. As well, ZmMYB59 expression extremely inhibited the synthesis of gibberellin A1 (GA 1 ) and cytokinin (CTK), and promoted the synthesis of abscisic acid (ABA) concurrently, which implied that seed germination was repressed by ZmMYB59 in hormone levels. Furthermore, cell length and cell number of hypocotyl/mesocotyl in transgenic plants were notably decreased. Conclusions Taken together, it proposed that ZmMYB59 plays a negative regulation during seed germination in tobacco and rice, which also contributes to illuminate the molecular mechanisms regulated by MYB transcription factors.

scholarly journals A MYB-related Transcription Factor from Sheepgrass, LcMYB2, Promotes Seed Germination and Root Growth under Drought Stress

2019 ◽  
Author(s):  
Pincang Zhao ◽  
Shenglin Hou ◽  
xiufang guo ◽  
Junting Jia ◽  
Weiguang Yang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Root Growth ◽  
Genetic Manipulation ◽  
Marker Genes ◽  
Plant Responses ◽  
Adverse Conditions ◽  
Related Transcription Factor

Abstract Background Drought is one of the most serious factors limiting plant growth and production. Sheepgrass can adapt well to various adverse conditions, including drought. However, during germination, sheepgrass young seedlings are sensitive to these adverse conditions. Therefore, the adaptability of seedlings is very important for plant survival, especially in plants that inhabit grasslands or the construction of artificial grassland. Results In this study, we found a sheepgrass MYB-related transcription factor, LcMYB2 that is up-regulated by drought stress and returns to a basal level after rewatering. The expression of LcMYB2 was mainly induced by osmotic stress and was localized to the nucleus. Furthermore, we demonstrate that LcMYB2 promoted seed germination and root growth under drought and ABA treatments. Additionally, we confirmed that LcMYB2 can regulate LcDREB2 expression in sheepgrass by binding to its promoter, and it activates the expression of the osmotic stress marker genes AtDREB2A, AtLEA14 and AtP5CS1 by directly binding to their promoters in transgenic Arabidopsis. Conclusions Based on these results, we propose that LcMYB2 improves plant drought stress tolerance by increasing the accumulation of osmoprotectants and promoting root growth. Therefore, LcMYB2 plays pivotal roles in plant responses to drought stress and is an important candidate for genetic manipulation to create drought-resistant crops, especially during seed germination.

scholarly journals CHOTTO1, a Putative Double APETALA2 Repeat Transcription Factor, Is Involved in Abscisic Acid-Mediated Repression of Gibberellin Biosynthesis during Seed Germination in Arabidopsis

2009 ◽  
Vol 151 (2) ◽  
pp. 641-654 ◽  
Author(s):  
Ryoichi Yano ◽  
Yuri Kanno ◽  
Yusuke Jikumaru ◽  
Kazumi Nakabayashi ◽  
Yuji Kamiya ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abscisic Acid ◽  
Seed Germination ◽  
Gibberellin Biosynthesis

scholarly journals AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA-induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5

2014 ◽  
Vol 38 (3) ◽  
pp. 559-571 ◽  
Author(s):  
JUN HYEOK KIM ◽  
WOO YOUNG HYUN ◽  
HOAI NGUYEN NGUYEN ◽  
CHAN YOUNG JEONG ◽  
LIMING XIONG ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Seed Germination ◽  
Bzip Transcription Factor ◽  
R2r3 Myb
Sign in / Sign up

Export Citation Format

Share Document