Respiratory burst oxidase homologue-dependent H2
O2
 and chloroplast H2
O2
 are essential for the maintenance of acquired thermotolerance during recovery after acclimation

Reactive oxygen species are a byproduct of aerobic metabolic processes but are also produced by plants in defense against pathogens. In addition, they can function as signaling molecules that control various aspects of plant life, ranging from developmental processes to responses to abiotic and biotic stimuli. In plants, reactive oxygen species can be produced by respiratory burst oxidase homologues. Arabidopsis contains 10 genes for respiratory burst oxidase homologues that are involved in different aspects of plant life. Plant pathogenic cyst nematodes such as Heterodera schachtii induce a syncytium in the roots of host plants that becomes a feeding site which supplies nutrients throughout the life of the nematode. In line with this function, the transcriptome of the syncytium shows drastic changes. One of the genes that is most strongly downregulated in syncytia codes for respiratory burst oxidase homologue B. This gene is root-specific and we confirm here the downregulation in nematode feeding sites with a promoter::GUS (β-glucuronidase) line. Overexpression of this gene resulted in enhanced resistance against nematodes but also against leaf-infecting pathogens. Thus, respiratory burst oxidase homologue B has a role in resistance. The function of this gene is in contrast to respiratory burst oxidase homologues D and F, which have been found to be needed for full susceptibility of Arabidopsis to H. schachtii. However, our bioinformatic analysis did not find differences between these proteins that could account for the opposed function in the interaction with nematodes.

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Root respiratory burst oxidase homologue-dependent H2O2 production confers salt tolerance on a grafted cucumber by controlling Na+ exclusion and stomatal closure

Journal of Experimental Botany ◽

10.1093/jxb/erx386 ◽

2017 ◽

Vol 69 (14) ◽

pp. 3465-3476 ◽

Cited By ~ 37

Author(s):

Mengliang Niu ◽

Yuan Huang ◽

Shitao Sun ◽

Jingyu Sun ◽

Haishun Cao ◽

...

Keyword(s):

Salt Tolerance ◽

Respiratory Burst ◽

Stomatal Closure ◽

H2o2 Production ◽

Respiratory Burst Oxidase ◽

Respiratory Burst Oxidase Homologue

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Respiratory burst oxidase homologue‐dependent H2O2 is essential during heat stress memory in heat sensitive tomato

Scientia Horticulturae ◽

10.1016/j.scienta.2019.108777 ◽

2019 ◽

Vol 258 ◽

pp. 108777 ◽

Cited By ~ 1

Author(s):

Mintao Sun ◽

Fangling Jiang ◽

Rong Zhou ◽

Junqin Wen ◽

Shouyao Cui ◽

...

Keyword(s):

Heat Stress ◽

Respiratory Burst ◽

Stress Memory ◽

Respiratory Burst Oxidase ◽

Respiratory Burst Oxidase Homologue

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RBOHF2 of Barley Is Required for Normal Development of Penetration Resistance to the Parasitic Fungus Blumeria graminis f. sp. hordei

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-23-9-1143 ◽

2010 ◽

Vol 23 (9) ◽

pp. 1143-1150 ◽

Cited By ~ 40

Author(s):

Reinhard K. Proels ◽

Kathrin Oberhollenzer ◽

Indira Priyadarshini Pathuri ◽

Götz Hensel ◽

Jochen Kumlehn ◽

...

Keyword(s):

Cell Death ◽

Respiratory Burst ◽

Penetration Resistance ◽

Blumeria Graminis ◽

Parasitic Fungus ◽

Powdery Mildew Fungus ◽

Morphological Alterations ◽

Hydrogen Peroxide Accumulation ◽

Respiratory Burst Oxidase ◽

Respiratory Burst Oxidase Homologue

Plant respiratory burst oxidase homologs are prominent sources of reactive oxygen species (ROS) in signal transduction and in interaction with microbes. However, the function of respiratory burst oxidase homologue (RBOH) genes in interaction with microbes might differ for certain plant and pathogen species. We produced transgenic barley knock down (KD) for the HvRBOHF2 isoform of NADPH oxidases. Young HvRBOHF2 KD shoots did not show obvious morphological alterations from the wild type but adult HvRBOHF2 KD plants developed fewer tillers, were less fertile, and showed spontaneous cell death in leaf mesophyll. Additionally, HvRBOHF2 KD plants were unable to contain wound-induced cell death. Before developmental failure became obvious, young HvRBOHF2 KD seedlings were much more susceptible to penetration by the biotrophic powdery mildew fungus Blumeria graminis f. sp. hordei. Strikingly, the B. graminis f. sp. hordei-induced cell-wall-associated oxidative burst was not substantially attenuated in HvRBOHF2 KD plants but enhanced susceptibility apparently influenced the subcellular site of hydrogen peroxide accumulation. Taken together, misexpression of HvRBOHF2 caused failure of barley to normally develop penetration resistance to B. graminis f. sp. hordei and to control leaf cell death.

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