Mitochondrial DNA 4977-base pair common deletion in blood leukocytes and melanoma risk

AbstractScar formation as a result of corneal wound healing is a leading cause of blindness. It is a challenge to understand why scar formation is more likely to occur in the central part of the cornea as compared to the peripheral part. The purpose of this study was to unravel the underlying mechanisms. We applied RNA-seq to uncover the differences of expression profile in keratocytes in the central/peripheral part of the cornea. The relative quantity of mitochondrial RNA was measured by multiplex qPCR. The characterization of mitochondrial RNA in the cytoplasm was confirmed by immunofluoresence microscope and biochemical approach. Gene expression was analyzed by western blot and RT qPCR. We demonstrate that the occurrence of mitochondrial DNA common deletion is greater in keratocytes from the central cornea as compared to those of the peripheral part. The keratocytes with CD have elevated oxidative stress levels, which leads to the leakage of mitochondrial double-stranded RNA into the cytoplasm. The cytoplasmic mitochondrial double-stranded RNA is sensed by MDA5, which induces NF-κB activation. The NF-κB activation thereafter induces fibrosis-like extracellular matrix expressions and IL-8 mRNA transcription. These results provide a novel explanation of the different clinical outcome in different regions of the cornea during wound healing.

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Analysis of Common Deletion (CD) and a novel deletion of mitochondrial DNA induced by ionizing radiation

International Journal of Radiation Biology ◽

10.1080/09553000701370878 ◽

2007 ◽

Vol 83 (7) ◽

pp. 433-442 ◽

Cited By ~ 34

Author(s):

Lu Wang ◽

Yoshikazu Kuwahara ◽

Li Li ◽

Taisuke Baba ◽

Ryong-Woon Shin ◽

...

Keyword(s):

Mitochondrial Dna ◽

Ionizing Radiation ◽

Common Deletion

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Oxidation‐ or Radiation‐Derived Effects on Human Mitochondria as Seen in a Fibroblast Cell Line Derived from a Family Known to Have Elevated Levels of the 4977‐bp Common Deletion in its Mitochondrial DNA

The FASEB Journal ◽

10.1096/fasebj.24.1_supplement.510.7 ◽

2010 ◽

Vol 24 (S1) ◽

Author(s):

Zachary Stoll ◽

Wendy Pogozelski

Keyword(s):

Mitochondrial Dna ◽

Cell Line ◽

Fibroblast Cell ◽

Fibroblast Cell Line ◽

Common Deletion

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Common Deletion (CD) in mitochondrial DNA of irradiated rat heart

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-37652014110912 ◽

2014 ◽

Vol 86 (2) ◽

pp. 685-694 ◽

Cited By ~ 1

Author(s):

RAQUEL G. SIQUEIRA ◽

DAYSE A. DA SILVA ◽

LUIZ D.B. DE MELO ◽

ELIZEU F. DE CARVALHO ◽

SAMARA C. FERREIRA-MACHADO ◽

...

Keyword(s):

Mitochondrial Dna ◽

Ionizing Radiation ◽

Real Time ◽

Real Time Pcr ◽

Rat Heart ◽

Wistar Rats ◽

Post Irradiation ◽

Male Wistar Rats ◽

The Common ◽

Common Deletion

The purpose of this study was to map the common deletion (CD) area in mtDNA and investigate the levels of this deletion in irradiated heart. The assays were developed in male Wistar rats that were irradiated with three different single doses (5, 10 or 15 Gy) delivered directly to the heart and the analyses were performed at various times post-irradiation (3, 15 or 120 days). The CDs area were sequenced and the CD quantified by real-time PCR. Our study demonstrated that the CD levels progressively decreased from the 3rduntil the 15th day after irradiation, and then increased thereafter. Additionally, it was observed that the levels of CD are modulated differently according to the different categories of doses (moderate and high). This study demonstrated an immediate response to ionizing radiation, measured by the presence of mutations in the CD area and a decrease in the CD levels.

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Recent Speciation Between the Baltimore Oriole and the Black-Backed Oriole

The Condor ◽

10.1093/condor/106.3.674 ◽

2004 ◽

Vol 106 (3) ◽

pp. 674-680 ◽

Cited By ~ 1

Author(s):

Beatrice Kondo ◽

Jason M. Baker ◽

Kevin E. Omland

Keyword(s):

Mitochondrial Dna ◽

Control Region ◽

Base Pair ◽

New World ◽

Molecular Evidence ◽

Cyt B ◽

Mtdna Sequences ◽

Male Plumage ◽

Fixed Base ◽

Average Sequence

Abstract A recent phylogenetic survey of the New World orioles (genus Icterus; Omland et al. 1999) suggested that the Baltimore Oriole (I. galbula) and the Black-backed Oriole (I. abeillei) are sister taxa. That survey examined mitochondrial DNA (mtDNA) from a single representative of each species in the genus. Here, we examine mtDNA sequences from 15 Black-backed and 20 Baltimore Orioles. The two species appear to be very recently diverged, with average sequence divergences for both cytochrome b (cyt b) and the control region indicating a probable late Pleistocene split. Despite this very recent divergence, there is one fixed base-pair difference between the species in cyt b and another in the control region, suggesting that one or both species have undergone a bottleneck during or since speciation. This molecular evidence of recent divergence suggests that male plumage differences between Black-backed and Baltimore Orioles evolved very rapidly. Especiación Reciente entre Icterus galbula y Icterus abeillei Resumen. Un estudio filogenético reciente de los orioles del Nuevo Mundo (género Icterus; Omland, et al. 1999) sugiere que Icterus galbula e I. abeillei son taxa hermanos. En aquel estudio se examinó el ADN mitocondrial de un sólo representante de cada especie del género. En este estudio examinamos secuencias de ADN mitocondrial de 15 individuos de I. abeillei y 20 de I. galbula. Las dos especies parecen haber divergido recientemente con una separación promedio de las secuencias nucleotídicas en citocromo b y la región de control que indica que la divergencia ocurrió probablemente a fines del Pleistoceno. A pesar de haber divergido tan recientemente, existe una diferencia fija de un par de bases en la secuencia nucleotídica entre las dos especies en citocromo b y otra diferencia fija en la secuencia nucleotídica de la región de control, lo cual sugiere que una o ambas especies han sufrido un efecto de cuello de botella desde o durante el proceso de especiación. Esta evidencia molecular de divergencia reciente sugiere que los elementos del plumaje en los machos evolucionaron muy rápidamente entre I. abeillei e I. galbula.

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