Impact of Brassica napus–Leptosphaeria maculans interaction on the emergence of virulent isolates of L. maculans , causal agent of blackleg disease in canola

2020 ◽  
Vol 70 (2) ◽  
pp. 459-474
Author(s):  
M. Harunur Rashid ◽  
Sakaria Liban ◽  
Xuehua Zhang ◽  
Paula Parks ◽  
Hossein Borhan ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Causal Agent ◽  
Blackleg Disease

RFLP mapping of resistance to the blackleg disease [causal agent, Leptosphaeria maculans (Desm.) Ces. et de Not.] in canola (Brassica napus L.)

1995 ◽  
Vol 91 (8) ◽  
pp. 1190-1194 ◽  
Author(s):  
Y. Dion ◽  
R. K. Gugel ◽  
G. F. W. Rakow ◽  
G. Séguin-Swartz ◽  
B. S. Landry
Keyword(s):  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Causal Agent ◽  
Rflp Mapping ◽  
Blackleg Disease ◽  
Brassica Napus L

scholarly journals Brassica napus Endophytes Have the Potential to Protect the Crop against the Blackleg Disease Causal Agent, the Fungus Leptosphaeria maculans

2020 ◽  
Author(s):  
Ezequiel Peral-Aranega ◽  
Zaki Saati-Santamaría ◽  
Raúl Rivas ◽  
Paula García-Fraile
Keyword(s):  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Causal Agent ◽  
Blackleg Disease

scholarly journals Limitations of transcriptome-based prediction of pathogenicity genes in the plant pathogen Leptosphaeria maculans

2019 ◽  
Vol 366 (7) ◽  
Author(s):  
Andrew S Urquhart ◽  
Alexander Idnurm
Keyword(s):  
Gene Expression ◽  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Simple Expression ◽  
Gene Transcript ◽  
Blackleg Disease ◽  
Fungal Pathogenicity ◽  
Transcript Levels ◽  
Oilseed Crops ◽  
High Gene

ABSTRACT Identification of pathogenicity determinants in Leptosphaeria maculans, a major cause of disease of oilseed crops, has been a focus of research for many years. A wealth of gene expression information from RNA sequencing promises to illuminate the mechanisms by which the fungus is able to cause blackleg disease. However, to date, no studies have tested the hypothesis that high gene transcript levels during infection correlate with importance to disease progression. In this study, we use CRISPR-Cas9 to disrupt 11 genes that are highly expressed during the early stages of disease and show that none of these genes are crucial for fungal pathogenicity on Brassica napus. This finding suggests that in order to understand the pathogenicity of this fungus more sophisticated techniques than simple expression analysis will need to be employed.

scholarly journals Genome-Wide Identification and Analysis of VQ Motif-containing Gene Family in Brassica napus and Functional Characterization of BnMKS1 in Response to Leptosphaeria maculans

2020 ◽  
Author(s):  
Zhongwei Zou ◽  
Fei Liu ◽  
Shuanglong Huang ◽  
DILANTHA GERARD FERNANDO
Keyword(s):  
Brassica Napus ◽  
Gene Family ◽  
Functional Characterization ◽  
Leptosphaeria Maculans ◽  
Defense Responses ◽  
Marker Genes ◽  
Blackleg Disease ◽  
Genome Wide ◽  
A Genome

Proteins containing Valine-glutamine (VQ) motifs play important roles in plant growth and development, as well as in defense responses to both abiotic and biotic stresses. Blackleg disease, which is caused by Leptosphaeria maculans, is the most important disease in canola (Brassica napus L.) worldwide. H; however, the identification of B. napus VQs and their functions in response to blackleg disease have not yet been reported. In this study, we conducted a genome genome-wide identification and characterization of the VQ gene family in B. napus, including chromosome location, phylogenetic relations, gene structure, motif domain, synteny analysis, and cis-elements categorization of their promoter regions. To understand B. napus VQ gene function in response to blackleg disease, we overexpressed BnVQ7 (BnaA01g36880D, also known as the mitogen-activated protein kinase4 substrate1 (MKS1) gene) in a blackleg-susceptible canola variety Westar. Overexpression The overexpression of BnMKS1 in canola did not improve its resistance to blackleg disease at the seedling stage. H; however, transgenic canola plants overexpressing BnMKS1 displayed an enhanced resistance to L. maculans infection at the adult plant stage. Expression levels of downstream and defense marker genes in cotyledons increased significantly at the necrotrophic stage of L. maculans infection in the overexpression line of BnMKS1, suggesting that the SA salicylic acid (SA)- and jasmonic acid (JA )-mediated signaling pathways were both involved in the defense responses. Together, these results suggest that BnMKS1 might play an important role in the defense against L. maculans.

Zinc suppresses sirodesmin PL toxicity and protects Brassica napus plants against the blackleg disease caused by Leptosphaeria maculans

Plant Science ◽  
1990 ◽  
Vol 68 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Thierry Rouxel ◽  
Albert Kollmann ◽  
Jean-Francois Bousquet
Keyword(s):  
Brassica Napus ◽  
Leptosphaeria Maculans ◽  
Blackleg Disease

scholarly journals Breakdown of a Brassica rapa subsp. sylvestris Single Dominant Blackleg Resistance Gene in B. napus Rapeseed by Leptosphaeria maculans Field Isolates in Australia

Plant Disease ◽  
2003 ◽  
Vol 87 (6) ◽  
pp. 752-752 ◽  
Author(s):  
H. Li ◽  
K. Sivasithamparam ◽  
M. J. Barbetti
Keyword(s):  
Brassica Napus ◽  
Resistance Gene ◽  
Disease Severity ◽  
Brassica Rapa ◽  
Oilseed Rape ◽  
Leptosphaeria Maculans ◽  
Blackleg Disease ◽  
Field Isolates ◽  
Western Australian ◽  
Lower Stem

Blackleg, caused by Leptosphaeria maculans, is a major disease of oilseed rape (Brassica napus) grown in Canada, Europe, and Australia. Cv. Surpass 400 was released in Australia in 2000 as the most resistant cultivar to L. maculans. It carries a single dominant resistance gene from B. rapa subsp. sylvestris. This cultivar usually shows a hypersensitive response to L. maculans characterized by small, dark brown lesions that are necrotic, localized, and without pycnidia on cotyledons, leaves, and stems. However, in 2001 on a Western Australian experimental farm, a small proportion of the lesions on the lower stem and crown region of cv. Surpass 400 were typical of those observed in susceptible cultivars, which were brown, necrotic lesions with a darker margin, but they contained fewer pycnidia. Forty seedlings of cv. Surpass 400 and susceptible cv. Westar were inoculated with pycnidiospore suspensions (106/ml) of each of 18 isolates taken from lesions on cv. Surpass 400. All 18 isolates caused collapse of cotyledons of susceptible cv. Westar. Four of these isolates caused large cotyledon lesions with some pycnidia on cv. Surpass 400. Three of these four isolates were subsequently inoculated onto 60 seedlings per isolate, at each of the four cotyledon lobes of each seedling of the two cultivars. Inoculated plants were assessed for disease severity on cotyledons and transplanted to the field 14 days after inoculation. The cotyledons of inoculated cv. Surpass 400 showed characteristic large, necrotic lesions with pycnidia, while the cotyledons of cv. Westar had collapsed and contained a mass of pycnidia. Blackleg disease severity in the crown region of the stem was assessed at 2 weeks before harvest. Fifty-four percent of the cv. Surpass 400 transplanted inoculated plants subsequently developed susceptible symptoms of crown cankers on stems. These symptoms were deep, girdling, brown lesions on the plant crowns with some pycnidia. One hundred percent of cv. Westar plants were infected and dead at this stage. This confirmed the ability of these field isolates to overcome the single dominant resistance gene present in cv. Surpass 400. To our knowledge, this is the first report of breakdown of a single dominant B. rapa subsp. sylvestris gene based resistance to blackleg in oilseed rape in the field.

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