RFLP mapping of resistance to the blackleg disease [causal agent, Leptosphaeria maculans (Desm.) Ces. et de Not.] in canola (Brassica napus L.)

1995 ◽  
Vol 91 (8) ◽  
pp. 1190-1194 ◽  
Author(s):  
Y. Dion ◽  
R. K. Gugel ◽  
G. F. W. Rakow ◽  
G. Séguin-Swartz ◽  
B. S. Landry
2020 ◽  
Vol 70 (2) ◽  
pp. 459-474
Author(s):  
M. Harunur Rashid ◽  
Sakaria Liban ◽  
Xuehua Zhang ◽  
Paula Parks ◽  
Hossein Borhan ◽  
...  

2020 ◽  
Author(s):  
Ezequiel Peral-Aranega ◽  
Zaki Saati-Santamaría ◽  
Raúl Rivas ◽  
Paula García-Fraile

1995 ◽  
Vol 75 (2) ◽  
pp. 437-439 ◽  
Author(s):  
G. R. Stringam ◽  
V. K. Bansal ◽  
M. R. Thiagarajah ◽  
D. F. Degenhardt ◽  
J. P. Tewari

The doubled haploid breeding method and greenhouse screening using cotyledon bio-assay were successfully applied to transfer blackleg resistance from the Australian cultivar Maluka (Brassicas napus), into susceptible advanced B. napus lines from the University of Alberta. This approach for blackleg resistance breeding was effective and efficient as several superior blackleg resistant breeding lines were identified within 4 yr from the initial cross. One of these lines (91–21864NA) was entered in the 1993 trials of the Western Canada Canola/Rapeseed Recommending Committee. Key words: Blackleg resistance, Leptosphaeria maculans, doubled haploid, Brassica napus


2019 ◽  
Vol 366 (7) ◽  
Author(s):  
Andrew S Urquhart ◽  
Alexander Idnurm

ABSTRACT Identification of pathogenicity determinants in Leptosphaeria maculans, a major cause of disease of oilseed crops, has been a focus of research for many years. A wealth of gene expression information from RNA sequencing promises to illuminate the mechanisms by which the fungus is able to cause blackleg disease. However, to date, no studies have tested the hypothesis that high gene transcript levels during infection correlate with importance to disease progression. In this study, we use CRISPR-Cas9 to disrupt 11 genes that are highly expressed during the early stages of disease and show that none of these genes are crucial for fungal pathogenicity on Brassica napus. This finding suggests that in order to understand the pathogenicity of this fungus more sophisticated techniques than simple expression analysis will need to be employed.


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