scholarly journals Epidemic of cutaneous fowlpox in a naïve population of chickens and turkeys in Austria: Detailed phylogenetic analysis indicates co‐evolution of Fowlpox virus with reticuloendotheliosis virus

2022 ◽  
Author(s):  
Miguel Matos ◽  
Ivana Bilic ◽  
Nicola Palmieri ◽  
Peter Mitsch ◽  
Franz Sommer ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Reticuloendotheliosis Virus ◽  
Fowlpox Virus

scholarly journals Reticuloendotheliosis Virus Sequences within the Genomes of Field Strains of Fowlpox Virus Display Variability

2003 ◽  
Vol 77 (10) ◽  
pp. 5855-5862 ◽  
Author(s):  
Pratik Singh ◽  
William M. Schnitzlein ◽  
Deoki N. Tripathy
Keyword(s):  
Cultured Cells ◽  
The United States ◽  
Reticuloendotheliosis Virus ◽  
Long Terminal Repeats ◽  
Field Sample ◽  
Fowlpox Virus ◽  
Natural Event ◽  
Tracheal Lesion

ABSTRACT Nine field strains of fowlpox virus (FPV) isolated during a 24-year span from geographically diverse outbreaks of fowlpox in the United States were screened for the presence of reticuloendotheliosis virus (REV) sequences in their genomes by PCR. Each isolate appeared to be heterogeneous in that either a nearly intact provirus or just a 248- or 508-nucleotide fusion of portions of the integrated REV 5′ and 3′ long terminal repeats (LTRs) was exclusively present at the same genomic site. In contrast, four fowlpox vaccines of FPV origin and three originating from pigeonpox virus were genetically homogeneous in having retained only the 248-bp LTR fusion, whereas two other FPV-based vaccines had only the larger one. These remnants of integrated REV presumably arose during homologous recombination at one of the two regions common to both LTRs or during retroviral excision from the FPV genome. Loss of the provirus appeared to be a natural event because the tripartite population could be detected in a field sample (tracheal lesion). Moreover, the provirus was also readily deleted during propagation of FPV in cultured cells, as evidenced by the detection of truncated LTRs after one passage of a plaque-purified FPV recombinant having a “genetically marked” provirus. However, the deletion mutants did not appear to have a substantial replicative advantage in vitro because even after 55 serial passages the original recombinant FPV was still prevalent. As to the in vivo environment, retention of the REV provirus may confer some benefit to FPV for infection of poultry previously vaccinated against fowlpox.

scholarly journals Field and Vaccine Strains of Fowlpox Virus Carry Integrated Sequences from the Avian Retrovirus, Reticuloendotheliosis Virus

Virology ◽  
1997 ◽  
Vol 235 (2) ◽  
pp. 367-376 ◽  
Author(s):  
Christian Hertig ◽  
Barbara E.H. Coupar ◽  
Allan R. Gould ◽  
David B. Boyle
Keyword(s):  
Reticuloendotheliosis Virus ◽  
Fowlpox Virus

scholarly journals Pathological, molecular and phylogenic study of fowlpox virus in domesticated chickens of Tikrit City, Iraq

2021 ◽  
Vol 58 ◽  
pp. e176255
Author(s):  
Ismael Ibrahim Hasan ◽  
Saad Tawfik Rasheed ◽  
Mohammed Khorshid Shakor
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Analysis ◽  
Core Protein ◽  
Chorioallantoic Membrane ◽  
Genetic Distances ◽  
Animal Tissue ◽  
Economic Losses ◽  
Cutaneous Lesions ◽  
Fowlpox Virus ◽  
Tissue Samples

Fowlpox virus (FPV) is one of the viruses affecting chickens worldwide, causing pathological and economic losses in the poultry industry. Viral lesions are easily recognizable by the eye and usually appear in the featherless areas, especially the head. Moreover, the virus could lead to blindness and mortality in some cases. This study diagnosed the suspected fowlpox cases, identified and classified the causative agent. We also analyzed the differences and similarities of closely related viruses at the neighboring and regional countries. Fifty samples were collected from three locations of Tikrit city from the domesticated chickens, which showed cutaneous lesions. Virus DNA was extracted directly from tissue samples before the nested PCR technique was performed. The virion core protein (P4b) gene is partially sequenced and analyzed with routine histological sectioning. Results showed that the virus causes pock lesions of dermal hyperplasia and hyperkeratosis. Hyperplasia and congestion of the chorioallantoic membrane were also recorded. The study also showed that the DNA of FPV could be extracted directly from animal tissue without further purification. The sequence analysis showed that the FPV was confirmed in all samples clustered in clade A identical with Iranian and Egyptian isolates. In conclusion, this study approved that the virus belongs to the classical dermal type of poxviruses and the short genetic distances between viruses related to closely neighboring countries. We also concluded that the conservative P4b gene included mutation sites that make this gene practical for diagnosing the virus and phylogenetic analysis.

scholarly journals Detection of Fowlpox virus carrying distinct genome segments of Reticuloendotheliosis virus

2019 ◽  
Vol 260 ◽  
pp. 53-59 ◽  
Author(s):  
Lok R. Joshi ◽  
Fernando V. Bauermann ◽  
Kyle S. Hain ◽  
Gerald F. Kutish ◽  
Anibal G. Armién ◽  
...  
Keyword(s):  
Reticuloendotheliosis Virus ◽  
Fowlpox Virus

Characterisation of an Australian fowlpox virus carrying a near-full-length provirus of reticuloendotheliosis virus

2021 ◽  
Author(s):  
Subir Sarker ◽  
Ajani Athukorala ◽  
Timothy R. Bowden ◽  
David B. Boyle
Keyword(s):  
Full Length ◽  
Reticuloendotheliosis Virus ◽  
Fowlpox Virus

Molecular characterization and phylogenetic analysis of the reticuloendotheliosis virus isolated from wild birds in Northeast China

2013 ◽  
Vol 166 (1-2) ◽  
pp. 68-75 ◽  
Author(s):  
Lili Jiang ◽  
Xiaole Qi ◽  
Yulong Gao ◽  
Yuping Hua ◽  
Kai Li ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Northeast China ◽  
Wild Birds ◽  
Reticuloendotheliosis Virus

First report on molecular characterization and phylogenetic analysis of Reticuloendotheliosis virus in Sudan

2020 ◽  
Vol 52 (4) ◽  
pp. 2073-2078 ◽  
Author(s):  
Shima H. Alfaki ◽  
Mohammed O. Hussien ◽  
Nussieba A. Osman ◽  
Khalid A. Enan ◽  
Abdel Rahim M. El Hussein
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Reticuloendotheliosis Virus ◽  
First Report

An atypical clinicopathological manifestation of fowlpox virus associated with reticuloendotheliosis virus in commercial laying hen flocks in Brazil

2020 ◽  
Vol 67 (6) ◽  
pp. 2923-2935
Author(s):  
Ruy D. Chacón ◽  
Claudete S. Astolfi‐Ferreira ◽  
David I. De la Torre ◽  
Lilian R. M. Sá ◽  
Antonio J. Piantino Ferreira
Keyword(s):  
Laying Hen ◽  
Reticuloendotheliosis Virus ◽  
Fowlpox Virus
Sign in / Sign up

Export Citation Format

Share Document