scholarly journals Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis

2013 ◽  
Vol 73 (5) ◽  
pp. 761-775 ◽  
Author(s):  
Yusuke Nakai ◽  
Yoichi Nakahira ◽  
Hiroki Sumida ◽  
Kosuke Takebayashi ◽  
Yumiko Nagasawa ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Stress Responses ◽  
Biotic Stress ◽  
Zinc Finger Protein ◽  
Vascular Plant ◽  
Abiotic And Biotic Stress ◽  
Finger Protein ◽  
Biotic Stress Responses

scholarly journals Vascular Plant One-Zinc-Finger (VOZ) Transcription Factors Are Positive Regulators of Salt Tolerance in Arabidopsis

2018 ◽  
Vol 19 (12) ◽  
pp. 3731 ◽  
Author(s):  
Kasavajhala Prasad ◽  
Denghui Xing ◽  
Anireddy Reddy
Keyword(s):  
Transcription Factors ◽  
Salt Stress ◽  
Zinc Finger ◽  
Stress Responses ◽  
Double Mutant ◽  
Zinc Finger Protein ◽  
Vascular Plant ◽  
Enrichment Analysis ◽  
Salt Stress Response ◽  
Positive Regulators

Soil salinity, a significant problem in agriculture, severely limits the productivity of crop plants. Plants respond to and cope with salt stress by reprogramming gene expression via multiple signaling pathways that converge on transcription factors. To develop strategies to generate salt-tolerant crops, it is necessary to identify transcription factors that modulate salt stress responses in plants. In this study, we investigated the role of VOZ (VASCULAR PLANT ONE-ZINC FINGER PROTEIN) transcription factors (VOZs) in salt stress response. Transcriptome analysis in WT (wild-type), voz1-1, voz2-1 double mutant and a VOZ2 complemented line revealed that many stress-responsive genes are regulated by VOZs. Enrichment analysis for gene ontology terms in misregulated genes in voz double mutant confirmed previously identified roles of VOZs and suggested a new role for them in salt stress. To confirm VOZs role in salt stress, we analyzed seed germination and seedling growth of WT, voz1, voz2-1, voz2-2 single mutants, voz1-1 voz2-1 double mutant and a complemented line under different concentrations of NaCl. Only the double mutant exhibited hypersensitivity to salt stress as compared to WT, single mutants, and a complemented line. Expression analysis showed that hypersensitivity of the double mutant was accompanied by reduced expression of salt-inducible genes. These results suggest that VOZ transcription factors act as positive regulators of several salt-responsive genes and that the two VOZs are functionally redundant in salt stress.

Single zinc-finger extension: enhancing transcriptional activity and specificity of three-zinc-finger proteins

2005 ◽  
Vol 386 (2) ◽  
pp. 95-99 ◽  
Author(s):  
Alexander E.F. Smith ◽  
Farzin Farzaneh ◽  
Kevin G. Ford
Keyword(s):  
Transcription Factors ◽  
Fusion Protein ◽  
Zinc Finger ◽  
Protein Interactions ◽  
Transcriptional Activation ◽  
Zinc Finger Protein ◽  
Zinc Finger Proteins ◽  
Finger Protein ◽  
Finger Extension ◽  
Vp16 Fusion

AbstractIn order to demonstrate that an existing zinc-finger protein can be simply modified to enhance DNA binding and sequence discrimination in both episomal and chromatin contexts using existing zinc-finger DNA recognition code data, and without recourse to phage display and selection strategies, we have examined the consequences of a single zinc-finger extension to a synthetic three-zinc-finger VP16 fusion protein, on transcriptional activation from model target promoters harbouring the zinc-finger binding sequences. We report a nearly 10-fold enhanced transcriptional activation by the four-zinc-finger VP16 fusion protein relative to the progenitor three-finger VP16 protein in transient assays and a greater than five-fold enhancement in stable reporter-gene expression assays. A marked decrease in transcriptional activation was evident for the four-zinc-finger derivative from mutated regulatory regions compared to the progenitor protein, as a result of recognition site-size extension. This discriminatory effect was shown to be protein concentration-dependent. These observations suggest that four-zinc-finger proteins are stable functional motifs that can be a significant improvement over the progenitor three-zinc-finger protein, both in terms of specificity and the ability to target transcriptional function to promoters, and that single zinc-finger extension can therefore have a significant impact on DNA zinc-finger protein interactions. This is a simple route for modifying or enhancing the binding properties of existing synthetic zinc-finger-based transcription factors and may be particularly suited for the modification of endogenous zinc-finger transcription factors for promoter biasing applications.

scholarly journals Grapevine Biotechnology: Molecular Approaches Underlying Abiotic and Biotic Stress Responses

10.5772/64872 ◽  
2016 ◽  
Author(s):  
Grace Armijo ◽  
Carmen Espinoza ◽  
Rodrigo Loyola ◽  
Franko Restovic ◽  
Claudia Santibáñez ◽  
...  
Keyword(s):  
Stress Responses ◽  
Biotic Stress ◽  
Abiotic And Biotic Stress ◽  
Molecular Approaches ◽  
Biotic Stress Responses

scholarly journals 1000. Down-Regulating the Epithelial Glycoprotein-2 Promoter Utilizing Engineered Zinc Finger Protein Transcription Factors

2006 ◽  
Vol 13 ◽  
pp. S385
Author(s):  
Willemijn M. Gommans ◽  
Pamela M.J. McLaughlin ◽  
Robbert Cool ◽  
Beatrice I. Lindhout ◽  
Bert van der Zaal ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Protein Transcription

scholarly journals Cloning the cDNA for a New Human Zinc Finger Protein Defines a Group of Closely Related Krüppel-like Transcription Factors

1998 ◽  
Vol 273 (43) ◽  
pp. 28229-28237 ◽  
Author(s):  
Nobukyuki Matsumoto ◽  
Friedrich Laub ◽  
Rafael Aldabe ◽  
Wen Zhang ◽  
Francesco Ramirez ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

2017 ◽  
Vol 12 (3) ◽  
pp. e1295907 ◽  
Author(s):  
Misaki Koguchi ◽  
Kanako Yamasaki ◽  
Tomoko Hirano ◽  
Masa H. Sato
Keyword(s):  
Heat Stress ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Vascular Plant ◽  
Stress Granules ◽  
Finger Protein
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