scholarly journals The chimeric mitochondrial gene orf182 causes non-pollen-type abortion in Dongxiang cytoplasmic male-sterile rice

2018 ◽  
Vol 95 (4) ◽  
pp. 715-726 ◽  
Author(s):  
Hongwei Xie ◽  
Xiaojue Peng ◽  
Mingjuan Qian ◽  
Yicong Cai ◽  
Xia Ding ◽  
...  
Keyword(s):  
Mitochondrial Gene ◽  
Male Sterile ◽  
Pollen Type ◽  
Cytoplasmic Male Sterile

scholarly journals Disruption of the mitochondrial gene orf352 partially restores pollen development in cytoplasmic male sterile rice

2021 ◽  
Author(s):  
Shiho Omukai ◽  
Shin-ich Arimura ◽  
Kinya Toriyama ◽  
Tomohiko Kazama
Keyword(s):  
Amino Acids ◽  
Mitochondrial Genome ◽  
Pollen Development ◽  
Mitochondrial Gene ◽  
Pollen Viability ◽  
Target Site ◽  
Transcription Activator ◽  
Male Sterile ◽  
Gene Encoding ◽  
Cytoplasmic Male Sterile

AbstractPlant mitochondrial genomes sometimes carry cytoplasmic male sterility (CMS)-associated genes. These genes have been harnessed in agriculture to produce high-yielding F1 hybrid seeds in various crops. The gene orf352 was reported to be an RT102-type CMS gene in rice (Oryza sativa), although a causal demonstration of its role in CMS is lacking. Here, we employed mitochondrion-targeted transcription activator-like effector nucleases (mitoTALENs), to knock out orf352 from the mitochondrial genome in the cytoplasmic male sterile rice RT102A. We isolated 18 independent transformation events in RT102A that resulted in genome editing of orf352, including its complete removal from the mitochondrial genome in several plants. Sequence analysis around the mitoTALEN target sites revealed the repair of their induced double-strand breaks via homologous recombination. Near the 5ʹ target site, repair involved sequences identical to orf284, while repair of the 3ʹ target site yielded various new sequences that generated new chimeric genes consisting orf352 fragments. Plants with a new mitochondrial gene encoding amino acids 179 to 352 of ORF352 exhibited the same shrunken pollen grain phenotype as RT102A, whereas plants either lacking orf352 or harboring a new gene encoding amino acids 211 to 352 of ORF352 showed partial rescue of pollen viability and germination, although they failed to set seed. These results demonstrated that disruption of orf352 partially restored pollen development, indicating that amino acids 179 to 210 from ORF352 may contribute to the establishment of pollen abortion.

Rearrangements in maize mitochondrial genes

Genome ◽  
1989 ◽  
Vol 31 (1) ◽  
pp. 160-168 ◽  
Author(s):  
Lino L. Fragoso ◽  
Scott E. Nichols ◽  
Charles S. Levings III
Keyword(s):  
Chromosomal Location ◽  
Mitochondrial Gene ◽  
Gene Mutations ◽  
Mitochondrial Genes ◽  
Male Sterile ◽  
Chimeric Genes ◽  
Cytoplasmic Male Sterile ◽  
Coding Regions

Recombination is a significant cause of maize mitochondrial gene mutations. Chimeric genes are often found in the mitochondria of cytoplasmic male-sterile maize. They are created by rearrangements involving intramolecular and intermolecular recombination. Herein we describe the effect of rearrangements on the chromosomal location, flanking and coding regions of several maize mitochondrial genes.Key words: recombination, organelle, chimeric genes.

Cloning and Expression Analysis of cMDH Gene Related to Cytoplasmic Male Sterile Wheat with Aegilops kotschyi Cytoplasm

2009 ◽  
Vol 35 (9) ◽  
pp. 1620-1627 ◽  
Author(s):  
Long-Yu ZHANG ◽  
Hong-Xia LI ◽  
Gai-Sheng ZHANG ◽  
Jun-Sheng WANG ◽  
Yan-Fen HAN ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Cloning And Expression ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

Fertility and Cytomorphology of Anthers in New Cytoplasmic Male-Sterile Lines of Chinese Cabbage(Brassica campestris L. ssp. pekinensis)

2012 ◽  
Vol 30 (1) ◽  
pp. 49
Author(s):  
Zhan SHI ◽  
Zheng-Jie WAN ◽  
Yue-Jin XU ◽  
Rui-Chang ZOU ◽  
Tao HUANG ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

scholarly journals Cosegregation of Single Genes Associated with Fertility Restoration and Transcript Processing of Sorghum Mitochondrial orf107 and urf209

Genetics ◽  
1998 ◽  
Vol 150 (1) ◽  
pp. 383-391 ◽  
Author(s):  
Hoang V Tang ◽  
Ruying Chang ◽  
Daryl R Pring
Keyword(s):  
Fertility Restoration ◽  
Mitochondrial Gene ◽  
Pollen Viability ◽  
Single Gene ◽  
Dominant Gene ◽  
Nuclear Gene ◽  
Male Sterile ◽  
Reading Frame ◽  
Transcript Processing ◽  
Backcross Populations

Abstract Defective nuclear-cytoplasmic interactions leading to aberrant microgametogenesis in sorghum carrying the IS1112C male-sterile cytoplasm occur very late in pollen maturation. Amelioration of this condition, the restoration of pollen viability, involves a novel two-gene gametophytic system, wherein genes designated Rf3 and Rf4 are required for viability of individual gametes. Rf3 is tightly linked to, or represents, a single gene that regulates a transcript processing activity that cleaves transcriptsof orf107, a chimeric mitochondrial open reading frame specific to IS1112C. The mitochondrial gene urf 209 is also subject to nucleus-specific enhanced transcript processing, 5′ to the gene, conferred by a single dominant gene designated Mmt1. Examinations of transcript patterns in F2 and two backcross populations indicated cosegregation of the augmented orf107 and urf209 processing activities in IS1112C. Several sorghum lines that do not restore fertility or confer orf107 transcript processing do exhibit urf209 transcript processing, indicating that the activities are distinguishable. We conclude that the nuclear gene(s) conferring enhanced orf107 and urf209 processing activities are tightly linked in IS1112C. Alternatively, the similarity in apparent regulatory action of the genes may indicate allelic differences wherein the IS1112C Rf3 allele may differ from alleles of maintainer lines by the capability to regulate both orf107 and urf209 processing activities.

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