scholarly journals Enhancer‐mediated reporter gene expression in Arabidopsis thaliana : a forward genetic screen

2021 ◽  
Author(s):  
Yuan Lin ◽  
Hainan Zhao ◽  
Magdalena Kotlarz ◽  
Jiming Jiang
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Reporter Gene ◽  
Genetic Screen ◽  
Reporter Gene Expression ◽  
Forward Genetic Screen

scholarly journals A Collection of Pre-mRNA Splicing Mutants in Arabidopsis thaliana

2020 ◽  
Vol 10 (6) ◽  
pp. 1983-1996 ◽  
Author(s):  
Tatsuo Kanno ◽  
Peter Venhuizen ◽  
Ming-Tsung Wu ◽  
Phebe Chiou ◽  
Chia-Liang Chang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Alternative Splicing ◽  
Reporter Gene ◽  
Rna Binding ◽  
Binding Protein ◽  
Genetic Screen ◽  
Mrna Splicing ◽  
Splicing Factors ◽  
Sequencing Data

To investigate factors influencing pre-mRNA splicing in plants, we conducted a forward genetic screen using an alternatively-spliced GFP reporter gene in Arabidopsis thaliana. This effort generated a collection of sixteen mutants impaired in various splicing-related proteins, many of which had not been recovered in any prior genetic screen or implicated in splicing in plants. The factors are predicted to act at different steps of the spliceosomal cycle, snRNP biogenesis pathway, transcription, and mRNA transport. We have described eleven of the mutants in recent publications. Here we present the final five mutants, which are defective, respectively, in RNA-BINDING PROTEIN 45D (rbp45d), DIGEORGE SYNDROME CRITICAL REGION 14 (dgcr14), CYCLIN-DEPENDENT KINASE G2 (cdkg2), INTERACTS WITH SPT6 (iws1) and CAP BINDING PROTEIN 80 (cbp80). We provide RNA-sequencing data and analyses of differential gene expression and alternative splicing patterns for the cbp80 mutant and for several previously published mutants, including smfa and new alleles of cwc16a, for which such information was not yet available. Sequencing of small RNAs from the cbp80 mutant highlighted the necessity of wild-type CBP80 for processing of microRNA (miRNA) precursors into mature miRNAs. Redundancy tests of paralogs encoding several of the splicing factors revealed their functional non-equivalence in the GFP reporter gene system. We discuss the cumulative findings and their implications for the regulation of pre-mRNA splicing efficiency and alternative splicing in plants. The mutant collection provides a unique resource for further studies on a coherent set of splicing factors and their roles in gene expression, alternative splicing and plant development.

scholarly journals Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nathan J. VanDusen ◽  
Julianna Y. Lee ◽  
Weiliang Gu ◽  
Catalina E. Butler ◽  
Isha Sethi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genetic Screen ◽  
Forward Genetics ◽  
Epigenetic Mark ◽  
Biological Processes ◽  
Dynamic Changes ◽  
Forward Genetic Screen ◽  
High Resource ◽  
Organ Systems

AbstractThe forward genetic screen is a powerful, unbiased method to gain insights into biological processes, yet this approach has infrequently been used in vivo in mammals because of high resource demands. Here, we use in vivo somatic Cas9 mutagenesis to perform an in vivo forward genetic screen in mice to identify regulators of cardiomyocyte (CM) maturation, the coordinated changes in phenotype and gene expression that occur in neonatal CMs. We discover and validate a number of transcriptional regulators of this process. Among these are RNF20 and RNF40, which form a complex that monoubiquitinates H2B on lysine 120. Mechanistic studies indicate that this epigenetic mark controls dynamic changes in gene expression required for CM maturation. These insights into CM maturation will inform efforts in cardiac regenerative medicine. More broadly, our approach will enable unbiased forward genetics across mammalian organ systems.

Reporter Gene Expression in G2 of the 1-Cell Mouse Embryo

1993 ◽  
Vol 156 (2) ◽  
pp. 552-556 ◽  
Author(s):  
Prahlad T. Ram ◽  
Richard M. Schultz
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Mouse Embryo ◽  
Reporter Gene Expression ◽  
Cell Mouse Embryo

Tetracycline-regulated reporter gene expression in the moss Physcomitrella patens

1996 ◽  
Vol 30 (1) ◽  
pp. 199-205 ◽  
Author(s):  
Mathias Zeidler ◽  
Christiane Gatz ◽  
Elmar Hartmann ◽  
Jon Hughes
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Physcomitrella Patens ◽  
Reporter Gene Expression

scholarly journals A 4kb Fragment of the Desmocollin 3 Promoter Directs Reporter Gene Expression to Parakeratotic Epidermis and Primary Hair Follicles

2007 ◽  
Vol 127 (1) ◽  
pp. 245-247 ◽  
Author(s):  
Anita J. Merritt ◽  
Kuichun Zhu ◽  
David R. Garrod ◽  
Martyn A.J. Chidgey
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Hair Follicles ◽  
Reporter Gene Expression
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