A molecular approach to maggot debridement therapy with Lucilia sericata and its excretions/secretions in wound healing

2021 ◽  
Author(s):  
Fatma Kubra Tombulturk ◽  
Gonul Kanigur‐Sultuybek
Keyword(s):  
Wound Healing ◽  
Lucilia Sericata ◽  
Molecular Approach ◽  
Maggot Debridement Therapy

The Physiology of Wound Healing by the Medicinal Maggot, Lucilia sericata

2010 ◽  
pp. 39-81 ◽  
Author(s):  
Yamni Nigam ◽  
Edward Dudley ◽  
Alyson Bexfield ◽  
A.Elizabeth Bond ◽  
Julie Evans ◽  
...  
Keyword(s):  
Wound Healing ◽  
Lucilia Sericata

scholarly journals A novel mechanism in maggot debridement therapy: protease in excretion/secretion promotes hepatocyte growth factor production

2011 ◽  
Vol 301 (6) ◽  
pp. C1423-C1430 ◽  
Author(s):  
Kenjiro Honda ◽  
Koji Okamoto ◽  
Yasuhiro Mochida ◽  
Kunihiro Ishioka ◽  
Machiko Oka ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Transforming Growth Factor ◽  
Femoral Vein ◽  
Transforming Growth Factor Β1 ◽  
Control Group ◽  
Drug Target Discovery ◽  
Maggot Debridement Therapy ◽  
Hepatocyte Growth

Maggot debridement therapy (MDT) is effective for treating intractable wounds, but its precise molecular mechanism, including the association between MDT and growth factors, remains unknown. We administered MDT to nine patients (66.3 ± 11.8 yr, 5 male and 4 female) with intractable wounds of lower extremities because they did not respond to conventional therapies. Significant increases of hepatocyte growth factor (HGF) levels were observed in femoral vein blood during 48 h of MDT ( P < 0.05), but no significant change was found for vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), transforming growth factor-β1 (TGF-β1), or tumor necrosis factor-α (TNF-α). We conducted NIH-3T3 cell stimulation assay to evaluate the relation between HGF and protease activity in excretion/secretion (ES) derived from maggots. Compared with the control group, HGF was significantly higher in the 0.05 μg/ml ES group ( P < 0.01). Furthermore, protease inhibitors suppressed the increase of HGF ( P < 0.05). The HGF expression was increased in proportion to the ES protein concentration of 0.025 to 0.5 μg/ml. In fact, ES showed stronger capability of promoting HGF production and less cytotoxicity than chymotrypsin or bromelain. HGF is an important factor involved in cutaneous wound healing. Therefore, these results suggest that formation of healthy granulation tissue observed during MDT results from the increased HGF. Further investigation to identify molecules enhancing HGF expression by MDT will contribute greatly to drug target discovery for intractable wound healing therapy.

Regulation of MMP 2 and MMP 9 expressions modulated by AP-1 (c-jun) in wound healing: improving role of Lucilia sericata in diabetic rats

2018 ◽  
Vol 56 (2) ◽  
pp. 177-186 ◽  
Author(s):  
Fatma Kübra Tombulturk ◽  
Tugba Soydas ◽  
Elif Yaprak Sarac ◽  
Matem Tuncdemir ◽  
Ender Coskunpinar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Rats ◽  
Lucilia Sericata

Health professionals' perceptions of maggot debridement therapy

2021 ◽  
Vol 30 (Sup9a) ◽  
pp. VIIi-VIIxi
Author(s):  
Carmen Pajarillo ◽  
Ronald A Sherman ◽  
Robert Sheridan ◽  
Lewis E Kazis
Keyword(s):  
Wound Healing ◽  
Qualitative Analysis ◽  
Health Professionals ◽  
Insurance Coverage ◽  
Maggot Debridement Therapy ◽  
Insurance Reimbursement ◽  
Tissue Removal ◽  
The Us ◽  
Dead Tissue ◽  
Theory Framework

Background: Maggot debridement therapy (MDT), or the use of maggots in dead tissue removal, has been shown to be beneficial in wound healing. Yet MDT in the US is often only used once conventional debridement methods have failed. Method: In this study, nine health professionals, experienced in MDT, were interviewed in order to identify and analyse the perceived societal barriers to MDT acceptance and usage in the US. Results: Through qualitative analysis, using the grounded theory framework, this study found that among those interviewed, insurance reimbursement restrictions and stigmatisation of medicinal maggots were the factors driving resistance to MDT use. Conclusion: Specifically, the ‘yuck’ factor and the perception of MDT as an ‘ancient’ modality contributed towards MDT stigma; in addition, lack of outpatient insurance coverage deterred MDT use. These findings provide useful information regarding the perceptual and systemic barriers that prevent greater acceptance of MDT. Ultimately, these barriers must be understood if we are to facilitate MDT implementation and improve MDT usage in the future.

Efficacy of Maggot Debridement Therapy on Refractory Atypical Diabetic Foot Ulcers: An Open-Label Study

2020 ◽  
pp. 153473462092040
Author(s):  
Mansour Siavash ◽  
Ali Najjarnezhad ◽  
Nader Mohseni ◽  
Seyed Mohammad Abtahi ◽  
Azadeh Karimy ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Foot ◽  
Bacterial Infections ◽  
Healing Time ◽  
Foot Ulcers ◽  
Diabetic Foot Ulcers ◽  
Open Label ◽  
Major Health Problem ◽  
Maggot Debridement Therapy ◽  
Complete Wound Healing

Atypical or refractory diabetic foot ulcers (DFUs) are still a major health problem. Maggot debridement therapy (MDT) by larva of Lucilia sericata is an ancient and a modern option for wound healing. It works by debridement, stimulation of wound healing, and disinfection. In this study, we aimed to evaluate the efficacy of MDT for healing atypical and refractory DFUs. Patients with atypical DFUs were selected and further evaluated for some predefined differential diagnoses like atypical fungal, parasitic, or bacterial infections, malignancy, trauma, and so on. Multiple MDT sessions were carried out. Ulcer size was measured before every MDT session. Complete wound healing, time to heal, and adverse effects were recorded as well. Forty-two DFU patients (26 men, 16 women) with 42 nonhealing atypical ulcers participated in this study. Complete wound healing was achieved in 35 patients (83.3%) by MDT. Complete debridement and then healing of the wounds happened in less than 1.79 ± 0.8 months. Four ulcers persisted, and 3 (7.1%) were eventually amputated. MDT may be considered as an effective treatment for atypical DFUs, which are unresponsive to conventional therapies.

Further Data on Wound Healing Rates After Application of Lucilia sericata

2019 ◽  
pp. 153473461987684
Author(s):  
Zbigniew Szczepanowski ◽  
Andrzej Tukiendorf ◽  
Grzegorz Krasowski
Keyword(s):  
Wound Healing ◽  
Healing Rate ◽  
Wound Care ◽  
Nonparametric Tests ◽  
Lucilia Sericata ◽  
Clinical Aspects ◽  
Wound Surface ◽  
Public Attention ◽  
Advanced Analysis ◽  
Maggot Therapy

Maggot therapy has recently received a special medical and public attention, and according to the specialists’ opinion, it takes us to the future of a wound care. Simultaneously, as new biomechanisms were discovered, statistical analyses of wound healing rates were conducted usually adopting simple parametric and nonparametric tests. In this study, based on a set of statistical methods, we performed an advanced analysis of wound surface reduction using Lucilia sericata larvae in different clinical aspects: status of diabetes mellitus, maggots’ density, and pain intensity. Particularly, we employed these factors because, in our statistical analysis, they are easy to obtain and they proved to be the possible risk factors of wound regeneration. Furthermore, these factors represent different clinical, biological, and neurological spectra of knowledge. In our study, we have found further and statistically significant correlations between the analyzed variables and skin regeneration together with different time periods of the healing rate using maggot therapy in patients with lower limb ulceration.

scholarly journals Wound healing potential: Evaluation of molecular profiling and amplification of Lucilia sericata angiopoietin-1 mRNA mid-part

2020 ◽  
Author(s):  
Hamzeh Alipour ◽  
Marziae Shahriari-Namadi ◽  
Saeedeh Ebrahimi ◽  
Mohammad Djaefar Moemenbellah-Fard
Keyword(s):  
Wound Healing ◽  
Conventional Polymerase Chain Reaction ◽  
Structural Features ◽  
Current Data ◽  
Lucilia Sericata ◽  
High Identity ◽  
High Prevalence ◽  
Chronic Ulcers ◽  
Maggot Therapy ◽  
Angiopoietin 1

Abstract Objective: High prevalence of chronic ulcers and burden of disease necessitate the increasingly significant production of new recombinant proteins in the world. The angiopoietin-1 enzyme is a part of growth factors group which is secreted by Lucilia sericata (Diptera: Calliphoridae) larvae when they are exposed to wounds to ensure maggot therapy. It is one of the most potent proteins in wound healing. Given its essential role, angiopoietin-1 gene of L. sericata was characterized which provided some basic information on its identity. Results: The mid-part of angiopoietin-1 mRNA sequence was thus characterized based on the design of disparate primers such as exon-exon junction, conserved regions, and specific region primers via conventional polymerase chain reaction (PCR). Its structural features was configured by in silico method. The sequence of mid-part (390 bp) of angiopoietin-1 was determined empirically, and BLAST analysis unravelled its high identity (85%) with the sequence of angiopoietin-1 mRNA of the larval housefly, Musca domestica . The homology of this enzyme also exhibited that its nucleic acid sequence was very similar to the domains of angiopoietin-1 in Lucilia cuprina . The current data are critical to evaluate the action of this enzyme in recombinant protein production in future molecular studies on wound healing.

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