Analysis of ITS DNA Sequences of Korean Oxalis Species (Oxalidaceae)

2007 ◽  
Vol 37 (4) ◽  
pp. 419-430 ◽  
Author(s):  
Ja Choon Koo ◽  
Mi Suk Chae ◽  
Jeoung Ki Lee ◽  
Sung Soo Whang
Phytotaxa ◽  
2019 ◽  
Vol 401 (3) ◽  
pp. 190
Author(s):  
ZHENYAN YANG ◽  
CHENGJIN YANG ◽  
YUNHENG JI

Paris variabilis, a new species from the Wumengshan Mountains, southwestern China, is described and illustrated. The new species is placed in Paris section Euthyra. The new taxon was determined to be most morphologically similar to P. vietnamensis but differs in its oblong leaf blades with an acute apex, stamens 2–4 × petal number, greenish yellow filaments and an enlarged, purplish red style base. The phylogenetic placement of this species was assessed based on nuclear ribosomal ITS DNA sequences data. The results of morphological and phylogenetic analyses support the status of the taxon as a new species.


Phytotaxa ◽  
2015 ◽  
Vol 227 (1) ◽  
pp. 83 ◽  
Author(s):  
Wai-Chao Leong ◽  
Tao Deng ◽  
Hang Sun ◽  
Ching-I Peng ◽  
Kuo-Fang Chung

Begonia palmata D. Don is one of the most widely distributed and morphologically variable species of Asian Begoniaceae. Examinations of its morphological variation indicate that two of its seven varieties, B. palmata var. difformis and B. palmata var. crassisetulosa, both distributed in the Gaoligong Mountain areas of Yunnan, China are indistinguishable and yet distinct from other varieties. Phylogenetic analyses using ITS DNA sequences further reveals that samples identifiable to these two varieties are also distantly related to samples of typical B. palmata. Based on these observations, we combine and elevate these two varieties to the status of species, Begonia difformis (Irmsch.) W.C. Leong, C.I Peng & K.F Chung, comb. & stat. nov..


Phytotaxa ◽  
2016 ◽  
Vol 275 (2) ◽  
pp. 112 ◽  
Author(s):  
GERARDO A. SALAZAR ◽  
CÉSAR CHÁVEZ-RENDÓN ◽  
ALEJANDRO DE ÁVILA B. ◽  
ROLANDO JIMÉNEZ-MACHORRO

Bletia mixtecana, a new species from Oaxaca, Mexico is described and illustrated. This species is florally similar to B. parkinsonii but differs in its aerial thickened stems (‘pseudobulbs’) and several floral attributes. A phylogenetic analysis of nuclear ribosomal ITS DNA sequences indicates that B. mixtecana and B. parkinsonii are not closely related, suggesting that floral similarity represents either parallelism or shared ancestral (symplesiomorphic) traits. The new species is a strict gypsophile known only from two populations and it qualifies as endangered based on the small number of known populations and individuals, high habitat specificity and the observed loss of plants at one of the two known locations.


2000 ◽  
Vol 104 (10) ◽  
pp. 1155-1163 ◽  
Author(s):  
Barbara Paulus ◽  
Nils Hallenberg ◽  
Peter K. Buchanan ◽  
Geoffrey K. Chambers

2020 ◽  
Vol 14 (2) ◽  
pp. 101-110
Author(s):  
Istiana Prihatini ◽  
◽  
I.L.G. Nurtjahjaningsih ◽  
Farah Aulya Faradilla ◽  
Suranto Suranto ◽  
...  

Austropuccinia psidii is a pathogenic fungus that causes rust in the Myrtaceae plant. The extensive plantation of the host of this fungus has increased the attack of fungal pathogen, therefore, will increase the threat tothe presence of Myrtaceae species around the globe including in Indonesia. This present study was aiming to detect and identify the presence of this pathogen by morphologicaland molecular observation. Morphological observationrevealed the presence of A. psidii urediniospores onsalam (Syzygium polyanthum) andkayuputih (Melaleuca cajuputi)leaves collected from the arboretum of the Indonesian Center for Forest Biotechnology and Tree Improvement (CFBTI), and the presence of teliospores on young Syzygium leaves. PCR amplification using specific primers of Ppsi1 / Ppsi6 succeeded in detecting the presence of A. psidii fungi Melaleuca and Syzygium showed by DNA amplicon length around 500bp. Efforts to obtain ITS DNA sequences to compare the molecular characteristics of fungi from two different hosts have been carried out, however, the sequencing electropherogram was unreadable, so the comparison can not be performed. This study reported that A. psidii is currently present in Myrtaceae species in Yogyakarta, therefore precaution efforts should be conducted to avoid economic and ecological impact from this pathogen.


Author(s):  
Monika Singh O. P. Sharma ◽  
Someshwar Bhagat

Fusarium phythopathogenic fungi is responsible for high economic loss of cereal food crop. The objective of this study was aimed at isolation, morphological and molecular identification of Fusarium species. 13 different Fusarium spp. i.e. F. solani, F. chlamydosporum, F. tabacinum, F. fujikuroi, F. oxysporum, F. verticillioides, F. brachygibbosum, Fusarium sp. and F. incarnatum were isolated and identified from diseased samples of chickpea, pigeonpea, rice, lentil and garden pea crop. Colony characteristics like colony color, colony growth diameters, mycelium type, sporulation, pigmentation, odour were obtained after culture purification. Shape, size and septation of microconidia and macroconidia, position, shape, occurrence and size of chlamydospores, conidiophore branching were examined microscopically. MS10, BI01 and KA14 isolates were slow growing, BI02 and UP07 were moderate growing and BI03, HA04, MS06, MS09, MS11 and KA(Gul)13 were fast to very fast growing on PDA after 7-10 days. Chlamydospores were found in most of the isolates. Colonies were abundant, loosely tufted, fluffy, pannose, vinaceous floccose, powdery and some were flat appressed, arachnoid. Pigmentation of most of the isolates was pinkish white to dark pink, carmine to violet in colour. Phylogenetic analysis was done by maximum likelihood method using the ITS-rDNA region of Fusarium isolates and multiple sequence alignment of ITS DNA sequences was done using Clustal_W program and all identified sequences were submitted in NCBI GenBank database.


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