RESPON PERTUMBUHAN TANAMAN JATI PLUS PERHUTANI UMUR 11 TAHUN TERHADAP INTENSITAS PENJARANGAN DAN TUMPANG SARI (STUDI KASUS DI BKPH BEGAL KPH NGAWI, PERHUTANI JAWA TIMUR)

Thinning is one of silvicultural management actions in maintaining and increasing growth and quality of standing stands. This study aims to determine growth response of selected teak clone to several intensities of thinning and intercropping. The method was a factorial completely randomized block design (RCBD) with thinning intensity (4 levels): 0% (control = A1), 25% (A2), 50% (A3) and 75% (A4) as the main plot, whileintercropping (2 levels), as the sub plot. The results showed that the Current Annual Increment (CAI) of diameter at breast height (CAI DBH), volume/tree (CAI vol/tree) and volume/hectare (CAI vol/ha) of stands gave a significant positive response to thinning intensity treatment. CAI DBH one year after thinning were 0.79 cm, 1.47 cm, and 2.46 cm; increased by 32%, 145% and 310% at the thinning intensity of 25%, 50% and 75% when they compared with the control (0.59 cm). CAI vol/ha of stand in the first year, namely 11.88 m3/ha, 14.04 m3/ha, and 16.78 m3/ha or with an increase of 9%, 29% and 54% respectively at an intensity of 25 %, 50% and 75% compared to the control.

PERTUMBUHAN BIBIT MALAPARIDARI PROVENAN UJUNG KULON PADA UMUR 5 BULAN DIPERSEMAIAN

Malapari (Pongamia pinnata) is one of tree species belonging to Family of Leguminosae. Malapari seed oil were known as potential source for biofuel. The previous study showed that Provenance from Taman Nasional Ujung Kulon Banten had highest oil content among provenances in Java. Seed exploration was carried out to determine variations among families on oil content and growth performance. This study was conducted to determine the variation among families on growth performance at nursery level. The seedlings were used as planting stocks for Progeny Test establishment. This study was arranged in randomized completely block design with 50 families, 10 seedlings per plot and repeated in 4 blocks resulting the total number of observation units were 2000 seedlings. Seedling survival rate, growth performance (height, diameter, leave number), and sturdiness ratio was measured monthly up to 5 months after sowing. Analyses of variance was used to find out differences among families. Correlation among characters/parameters was analyzed by Pearson Correlation Analyses. The results showed that variations among families were found on seedling growthperformance. The seedling survival rate at the age of 5 months was 84.60% (26,70-100%) with an average growth of 47.10 cm (31,2-59,7 cm) in height, 5.49 mm (4,7-6,5 mm) in diameter, 8.56 for seedlings sturdiness and 15.4 (10,9-18,8) for leave number

MULTIPLIKASI Aquilaria malaccensisDENGAN NAA DAN RAGI PADA KULTUR IN VITRO

Multiplication of Aquilaria malaccensis with naa and yeast growth regulators on in vitro culture. This study aims to obtain the best concentration of NAA and yeast extract for multiplication of agarwood on in vitro culture. This research was conducted from January to October 2020 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas SebelasMaret, Surakarta. The experimental design used was a factorial completely randomized design (CRD) with twofactors, namely NAA (0 ppm; 0.1 ppm; 0.2 ppm; and 0.3 ppm) and yeast extract (0 mg/l, 700 mg/l, 800 mg/l, and 900 mg/l).(+)The results showed that the combination of 0 ppm NAA and 900 mg/lyeast increasedthe number of shoots of A.malaccensis explants with the highest average number of 3.67 shoots. A single NAA concentration of 0 ppm was able to increase the number of leaves of explants of A. malaccensis with the highest average leaf rate of 24.5 leaves. A single yeast concentration of 0 mg/lwas able to increase the number of leaves of A. malaccensis explants with an average of 22 leaves.

ISOLASI NON-DESTRUKTIF DAN DESTRUKTIF GEN COI PADA SERANGGA JENIS COLEOPTERA PEMBAWA PATOGEN CERATOCYSTIS

Identification of insect species using molecular approach is one of the first steps in managing Ceratocystis. Proper species identification of the vectorsof Ceratocystiswill provide an effective way in limiting the distribution of this pathogenic fungi. The isolation of insect DNA is a crucial step in species identification using molecular characters. The aim of this research was to obtain the most effective method for isolating the COI gene and to confirm the insect DNA using amplification of the DNA through polymerase chain reaction (PCR). Twelve unidentified Coleoptera specimen collected from Acacia spp. plantation in Pelalawan Riau were randomly selected for this study. The insect DNA were isolated using the CTAB buffer with four different pre-incubation treatments, involved a non destructive method, i.e. soaking (R), destructive methods, i.e. crushing (H), soaking-crushing (RH) and freezing-crushing (FH). Two sets of primer, LCO1490/HCO2196 and LCO1490/HCO2198 were used to amplify the DNA of COI gene. The results shows, the COI gene was isolated from all pre-incubation treatments, except in the non-destructive treatment. The isolated DNA of COI gene was successfully amplified using both primer sets used in this study

INVENTARISASI, KARAKTERISASIMORFOLOGI, DAN UJI VIABILITAS BENIHPACAR TERE (Impatiens platypetala Lindl.)HASIL EKSPLORASI DI KAWASAN TAMAN NASIONAL BROMO TENGGER SEMERU

BromoTenggerSemeruNationalPark(BTSNP)isoneofnaturalconservationareasinEastJavawitharound1,025plantspecies.Impatiensplatypetala,locallyknownaspacartere,isaplantspeciesmostlybefoundinmountainousareasofJavaandwithhighpotentialasmedicinalandornamentalplant.However,researchonthisspeciesisstilllimited.Thisstudywasconductedtoinventory,describethemorphologicalcharacteristicsandtesttheseedviabilityofpacartereinfourresortsofBTSNP,namelyRPTNGunungPenanjakan,CobanTrisula,Senduro,andRanuDarungan.Surveymethodwasusedtoinventorytheplantpopulations.Directfieldobservationwasusedtocharacterizetheplantmorphology.Seedsviabilitytestwasconductedthroughgerminationonstrawpapermedia.TheresultsshowedthatpacarterepopulationswerefoundinthosefourBTSNPresorts,onshadeandhumidhabitats,alsoinopenareaswithwetsoilconditions.Theplantischaracterizedasanerectparennialherb,spursedpinkflower,capsulafruitwhichdehiscentwhenripe,withflattenedcone-shapedandsmoothhairyseeds.Seedgerminationtestshowedthattheseedsbegantogerminateonday5aftersowing(around20sprouts/day),epigealtype,withpercentageofgerminationandviabilitywas56%and90%respectively.ResultsofthisstudyareexpectedtoprovidebasicinformationtosupporttheconservationeffortsofI.platypetalabothin-situandex-situ(seedandlivingplant),alsoitspropagationeffortsforfurtherbioprospectingresearches

PERTUMBUHAN AWAL KAYU MERAH (Pterocarpus indicus Willd) PADA PLOT KONSERVASI EX-SITU DI GUNUNGKIDUL UMUR 2 TAHUN

Ex-situ conservation plot of kayu merah (Pterocarpus indicus Willd) has been established in Gunungkidul in2016. The purpose of this study was to observe the early growth of kayu merah in the plot at 2 years old. The plot was laid-out in randomized complete block design consisting of 68 families, 4 tree-plot and 5 blocks with spacing of 3 m × 3 m. Genetic materials of kayu merah were collected from 3 populations namely Timor, Flores and Seram. Measurement was conducted in survival rate, tree diameter, tree height and number of branches. The result showed that the average survival rate of family was high (≥80%). Based on the populations, the survival rate of Timor, Flores and Seram were around 95%, 96% and 96.5% respectively. The average height, diameter and number of branches were 275 cm, 2.79 cm and 1.66, respectively. There were significant differences for height, diameter and number of branches among the tested families. In general, family ranking varied among the measured traits. Correlation between the traits varied among the populations. The Seram population showed the top family ranking for all the traits. It indicated that characterization in ex-situ conservation plot using genetic materials collected from the three populations was important for further conservation program of kayu merah.

DETEKSI DAN IDENTIFIKASI Austropuccinia psidii PADA MYRTACEAE DI YOGYAKARTAINDONESIA

Austropuccinia psidii is a pathogenic fungus that causes rust in the Myrtaceae plant. The extensive plantation of the host of this fungus has increased the attack of fungal pathogen, therefore, will increase the threat tothe presence of Myrtaceae species around the globe including in Indonesia. This present study was aiming to detect and identify the presence of this pathogen by morphologicaland molecular observation. Morphological observationrevealed the presence of A. psidii urediniospores onsalam (Syzygium polyanthum) andkayuputih (Melaleuca cajuputi)leaves collected from the arboretum of the Indonesian Center for Forest Biotechnology and Tree Improvement (CFBTI), and the presence of teliospores on young Syzygium leaves. PCR amplification using specific primers of Ppsi1 / Ppsi6 succeeded in detecting the presence of A. psidii fungi Melaleuca and Syzygium showed by DNA amplicon length around 500bp. Efforts to obtain ITS DNA sequences to compare the molecular characteristics of fungi from two different hosts have been carried out, however, the sequencing electropherogram was unreadable, so the comparison can not be performed. This study reported that A. psidii is currently present in Myrtaceae species in Yogyakarta, therefore precaution efforts should be conducted to avoid economic and ecological impact from this pathogen.

OPTIMASI DETEKSI GEN PADA Stelechocarpus burahol (Bl.) Hook.f. & Th. MENGGUNAKAN DIRECT KIR PCR

DNA isolation and purification in the conventional Polymerase Chain Reaction (PCR) process require reagents that are toxic, more costly and time consuming, and contamination. S. burahol leaves contain phenolics, flavonoids, and terpenoids which can interfere with DNA isolation. The use of direct PCR kits can detect genes without DNA extraction.The objective of study was to determinethe method of gene detection ofStelechocarpus buraholusing directPCRkit.In each location, one tree was taken as a source of leaf samples from Garut, Purwodadi Botanical Gardens, Kyai Langgeng Gardens, Yogyakarta Palace, Turi Sleman, Wanagama, Karanganyar, and South Kalimantan, except Bogor Botanical Gardens, two trees were taken. The primers used for the trials were ITS 1F primers and 4R primers. In the sequencing stage, PCR product samples of 40 -50 μl that showed positive results were detected by electrophoresis. The PCR product was measuredat ± 750 bp from ten samples. Direct PCR kits can be used for S. buraholgene detection, time and energy efficient, only requires a small amount of tissue, and reduces contamination due to DNA extraction. Direct PCR kits can be an effective method that can be utilized to detect target genesfor large populations.

PERTUMBUHAN STEK BATANG GMELINA ARBOREA HASIL KOLEKSI DARI LIMA POPULASI SEBARAN DI INDONESIA

his studywas conducted to determine the effect of plant material origin and size of cuttings on the growth ability of Gmelina. arborea stem cuttings in the nursery. The study was arranged in a randomized factorial pattern design, consisting of two factors, namely the length of the cuttings (L1 = 20 cm and L2 = 30 cm) and the cuttings size factor (small diameter class/D1 = <1.25 cm, medium/D2 = 1.25 –2.50 cm and large/D3 => 2.50 cm). Each treatment used 5 stem cuttings and repeated in 3 times, which are grouped based on their origin population namely Bantul/P1, Bogor/P2, Lampung/P3,Bondowoso/P4 and Lombok/P5. The observations showed the survival percentage was 83.12% and the rooting percentage was 78.23% which was influenced by the length and diameter of the stem cuttings. The growth of shoots (number, length and diameter) and the number of shoot nodes and leaf were affected by the interaction of origin of the population and the length and diameter of the stem cuttings.Applying stem cutting method is very potential to produce planting stock of Gmelina arbore. Stem cuttings which has a biger size showed better grow of stem cuttings.

INDUKSI KALUS EMBRIOGENIK DAN EMBRIO SOMATIK DARI EKSPLAN DAUN KULIM (Scorodacarpus borneensis Becc.)

Kulim is one of woody plant that have multifunction as wood source and for spice and medicinal. Generative propagation of this plant have trouble because seed use limited. The use of leaf segment through somatic embryogenesis to solve the problem. The objective of this study is to obtain the best treatment to embryogenic callus induction. The modification of basal medium of Murashige and Skoog was used as growth medium. The experiment was conducted in three stages are callus induction, embryogenic callus and somatic embryo induction. The treatment of 2,4-D (3,0 – 12 mg/l) used for callus induction. For embriogenic callus induction used 2,4-D (3,0 – 12,0 mg/l) combined with NAA 0,5 mg/l. The treatment of thidiazuron (0,1 – 0,7 mg/l) used for somatic embryo induction. The result showed that the treatment of 2,4-D 6,0 mg/l is the best for callus induction with compact of texture, green, dry and non embryogenic. The treatment of combination 2,4-D 12.0 mg/l with NAA 0.5 mg/l is the best for friable callus induction. The treatment of 2,4-D 6.0 mg/l combined with NAA 0,5 mg/l is the best for embryogenic callus induction with very friable of texture, easy to separate, dry, smooth and glossy. Thidiazuron of 0,1 mg/l treatment is the best for somatic embryos induction with the average number of 7,8 somatic embryos.