Sodium-dependent magnesium uptake by ferret red cells.

We have studied the cation content of rabbit erythrocytes; for a group of 40 animals red cell sodium was 9.2 ± 2.7 (S.D.) mmol/1 of cells, while potassium was 112 ± 8.6 mmol/1. Cell sodium content rose as the animals aged, but there was always a wide concentration gradient across the cell wall. This gradient was maintained by an active sodium pump, inhibited by ouabain (10−4 M) and comparable to pump I in the human red cell. The rate constant for this process in 16 rabbits was 0.313 ± 0.07 (S.D.) h−1, a value similar to that seen in man. Ethacrynic acid (10−3 M) inhibited a further component of sodium efflux, the rate constant being 0.259 ± 0.015 (S.D.) h−1. This was superficially comparable to pump II as previously described in the human; on further study, however, it was found to be sodium dependent, but able to function in the absence of adenosine triphosphate and incapable of net up-hill transport. These findings indicate that there is only one active transport mechanism in the red cells of the rabbit, which is a useful model for study in comparison to the red cells of man.

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Contamination of salvaged maternal blood by amniotic fluid and fetal red cells during elective Caesarean section

Yearbook of Anesthesiology and Pain Management ◽

10.1016/s1073-5437(08)79057-0 ◽

2009 ◽

Vol 2009 ◽

pp. 250-251

Author(s):

D.H. Chestnut

Keyword(s):

Caesarean Section ◽

Amniotic Fluid ◽

Elective Caesarean Section ◽

Maternal Blood ◽

Red Cells

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Differential Outcomes for Long Term ex vivo Lung Perfusion in a Porcine Model - With or without Red Cells?

The Thoracic and Cardiovascular Surgeon ◽

10.1055/s-0035-1544536 ◽

2015 ◽

Vol 63 (S 01) ◽

Author(s):

W. Sommer ◽

M. Avsar ◽

J. Salman ◽

C. Kühn ◽

I. Tudorache ◽

...

Keyword(s):

Porcine Model ◽

Ex Vivo ◽

Lung Perfusion ◽

Red Cells ◽

Ex Vivo Lung Perfusion ◽

Differential Outcomes

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Liquoid Induced Stasis and the Generalized Shwartzman Reaction

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646840 ◽

1979 ◽

Vol 41 (04) ◽

pp. 804-810 ◽

Cited By ~ 1

Author(s):

Knut Nordstoga

Keyword(s):

Red Cells ◽

Renal Lesions ◽

Intravenous Injections ◽

Shwartzman Reaction ◽

Glomerular Capillaries

SummaryThe composition of the occlusive material within dilated glomerular capillaries, following intravenous injections of Liquoid in blue foxes, was studied electron microscopically; it was found that it mainly consisted of a debris in which disintegrated red cells constituted the major component. Damaged platelets and necrotic endothelial remnants were other components. These observations were interpreted as a result of glomerular stasis, and it was concluded that stasis in glomerular capillaries is a basic event in the development of the renal lesions accompanying the generalized Shwartzman reaction.

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Receptor Mediated Binding of the Fibrinolytic Components, Plasminogen and Urokinase, to Peripheral Blood Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646019 ◽

1987 ◽

Vol 58 (03) ◽

pp. 936-942 ◽

Cited By ~ 96

Author(s):

Lindsey A Miles ◽

Edward F Plow

Keyword(s):

T Cells ◽

B Cell ◽

Peripheral Blood ◽

Binding Sites ◽

Blood Cells ◽

High Capacity ◽

Red Cells ◽

Peripheral Blood Cells ◽

Cellular Functions ◽

Fibrinolytic Proteins

SummaryGlu-plasminogen binds to platelets; the monocytoid line, U937, and the human fetal fibroblast line, GM1380 bind both plasminogen and its activator, urokinase. This study assesses the interaction of these fibrinolytic proteins with circulating human blood cells. Plasminogen bound minimally to red cells but bound saturably and reversibly to monocytes, granulocytes and lymphocytes with apparent Kd values of 0.9-1.4 μM. The interactions were of high capacity with 1.6 to 49 × 105 sites/cell and involved the lysine binding sites of plasminogen. Both T cells and non-rosetting lymphocytes and two B cell lines saturably bound plasminogen. Urokinase bound saturably to gianulocytes, monocytes, non-rosetting lymphocytes and a B cell line, but minimally to T cells, platelets and red cells. Therefore, plasminogen binding sites of high capacity, of similar affinities, and with common recognition specificities are expressed by many peripheral blood cells. Urokinase receptors are also widely distributed, but less so than plasminogen binding sites. The binding ol plasminogen and/ or urokinase to these cells may lead to generation of cell- associated proteolytic activity which contributes to a variety of cellular functions.

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Antithrombin Activity of Intact Human Platelets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651361 ◽

1975 ◽

Vol 34 (01) ◽

pp. 115-126 ◽

Cited By ~ 3

Author(s):

Kiyoake Watanabe ◽

Francis C Chao ◽

James L Tullis

Keyword(s):

Antithrombin Iii ◽

Human Platelets ◽

Significant Loss ◽

Red Cells ◽

Antithrombin Activity ◽

Α2 Macroglobulin ◽

Rapid Reaction ◽

Pre Treatment ◽

Different Characteristics

SummaryAntithrombin activity has been identified in intact washed human platelets. An apparent activity was demonstrated at platelet concentrations above 0.31 × 109/ml, when platelet suspensions were incubated with 2.0 NIH units/ml of thrombin. Neither red cells nor white cells revealed antithrombin activity. No significant loss of the platelet antithrombin activity was observed after ten successive washings or after treatment of platelets with antibodies to antithrombin III or α2-macroglobulin. Almost the same amount of antithrombin activity as normal platelets was demonstrated in the platelets from an afibrinogenemic patient. Pre-treatment of platelets with trypsin, papain, and neuroaminidase reduced the activity significantly, whereas lipase was without effect. The platelet antithrombin reacted with thrombin in less than 3 seconds, and this rapid reaction of platelet antithrombin was different from that of plasma antithrombin III or fibrinogen. The thrombin-like clotting activity of ancrod was inhibited by fibrinogen but not platelets. Also, unlike plasma antithrombin III or fibrinogen, brief exposure to heat (56° C or 60° C) reduced considerable amounts of platelet antithrombin activity. These results suggest that platelets possess a specific antithrombin with different characteristics from other known antithrombins.

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In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651281 ◽

1968 ◽

Vol 20 (03/04) ◽

pp. 384-396 ◽

Cited By ~ 1

Author(s):

G Zbinden ◽

S Tomlin

Keyword(s):

Platelet Adhesion ◽

Sodium Citrate ◽

Blood Platelets ◽

In Vitro Assay ◽

Red Cells ◽

Platelet Adhesiveness ◽

Vitro Assay ◽

In Vitro System ◽

Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

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