A Platform for Cross-Disciplinary Microchannel Research

2003 ◽  
Author(s):  
Scott Spearing ◽  
Sang Young Son ◽  
Jeffrey Allen ◽  
Lisa A. Monaco
Keyword(s):  
Structural Biology ◽  
Pressure Control ◽  
Protein Crystal ◽  
Biological Research ◽  
Development Effort ◽  
Original Objective ◽  
Gas Liquid Flow ◽  
Biology Group ◽  
Configuration Control ◽  
Biology Research

A team from the structural biology group located at the Marshall Space Flight Center in Huntsville Alabama is developing a platform suitable for cross-disciplinary microchannel research. The original objective of this engineering development effort was to deliver a multi-user flight-certified facility for iterative investigations of protein crystal growth; that is, Iterative Biological Crystallization. However, the unique capabilities of this facility are not limited to the low-gravity structural biology research community. Microchannel-based research in a number of other areas may be greatly accelerated through use of this facility. In particular, the potential for gas-liquid flow investigations and cellular biological research utilizing the exceptional pressure control and simplified coupling to macroscale diagnostics inherent with the facility will be discussed. Also noted will be the opportunities for research-specific modifications to the microchannel configuration, control and diagnostics.

BIOBOARD

2012 ◽  
Vol 16 (06) ◽  
pp. 5-13
Keyword(s):  
Structural Biology ◽  
H5n1 Virus ◽  
Gold Medal ◽  
Zinc Treatment ◽  
Dual Chamber ◽  
Heart Attacks ◽  
Joint Development ◽  
Living Lab ◽  
Biology Research ◽  
Made In

INDIA – Zinc Treatment for Infections in Children. JAPAN – Japan Pharma Portfolio Finds Renewed Strength through Record Growth in 2012. JAPAN – Joint Development of a Novel Lyophilized Dual Chamber Prefillable Syringe System. JAPAN – A Genetic Alternative to Fertilizer. SINGAPORE – Made-in-Singapore H5N1 Bird Flu Diagnostic Kit - Detects All Known Strains of H5N1 Virus with a Single Test. THAILAND – KEEEN, Thai Bioremediation Product, Awarded Gold Medal for "Bioremediation Agent developed to Greenovation Product". EUROPE – MorphoSys Antibody Reaches Major Milestone in Collaboration with Roche. EUROPE – New Technique to Predict Heart Attacks. USA – FEI Launches 'Living Lab' for Structural Biology Research at NIH. USA – Synthetic Platelets Built to Treat Bleeding. USA – Software Scans Tongue for Signs of Disease. USA – US Legislation Will Ensure Tighter Checks on Foreign Drug Factories.

scholarly journals NE-CAT: crystallography beamlines for challenging structural biology research

2019 ◽  
Vol 75 (a1) ◽  
pp. a120-a120
Author(s):  
Surajit Banerjee ◽  
Malcolm Capel ◽  
Igor Kourinov ◽  
Anthony Lynch ◽  
Frank Murphy ◽  
...  
Keyword(s):  
Structural Biology ◽  
Biology Research

scholarly journals NE-CAT: Crystallography Beamlines for Challenging Structural Biology Research

2018 ◽  
Vol 114 (3) ◽  
pp. 524a
Author(s):  
Surajit Banerjee ◽  
Malcolm Capel ◽  
Igor Kourinov ◽  
Anthony Lynch ◽  
Frank Murphy ◽  
...  
Keyword(s):  
Structural Biology ◽  
Biology Research

scholarly journals Structural investigation of cyclic nucleotide binding proteins from Trypanosoma cruzi

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Gabriel Ferri ◽  
Martin Edreira ◽  
Ivan Campeotto
Keyword(s):  
Life Cycle ◽  
Structural Biology ◽  
Structural Investigation ◽  
Buenos Aires ◽  
Therapeutic Agents ◽  
Parasite Life Cycle ◽  
Structure Based Drug Design ◽  
Antiparasitic Drugs ◽  
Nucleotide Binding Proteins ◽  
Biology Group

Fora targeted therapy of Trypanosomiasis, new antiparasitic drugs should be specifically directed against essential pathways in the parasite life cycle. Among these potential targets are signal transduction pathways, which have remained largely unexplored in Trypanosoma species. Of special interest is cAMP-mediated signaling, since cAMP has been shown to play critical roles in the life cycle of T. cruzi and in host cell during invasion. The presented research focuses on the identification and characterisation of novel cAMP response proteins (CARPs) in T. cruzi by using a multi disciplinary approach involving the parasitology group of Dr Martin Edreira (University of Buenos Aires, Argentina) and the structural biology group of Dr Ivan Campeotto (University of Leicester, UK). The aim of the project is not only to increase our knowledge about T. cruzi biology but also to target CARPs for the design and development of novel therapeutic agents against Chagas disease. To date, protein crystals of one of the members of the CARP family have been obtained, paving the way for structure determination and for a structure-based drug design approach.

scholarly journals NE-CAT crystallography beamlines for challenging structural biology research

2011 ◽  
Vol 67 (a1) ◽  
pp. C482-C483 ◽  
Author(s):  
I. Kourinov ◽  
S. E. Ealick ◽  
M. Capel ◽  
S. Banerjee ◽  
F. Murphy ◽  
...  
Keyword(s):  
Structural Biology ◽  
Biology Research

Size Based Particle Separation Method by Zero Diffusivity

2011 ◽  
Author(s):  
Chungmin Han ◽  
Jaesung Park
Keyword(s):  
Narrow Range ◽  
Dna Separation ◽  
Biological Research ◽  
Total System ◽  
Micro Scale ◽  
Nano Fabrication ◽  
Charge Dependence ◽  
Laminar Flow Control ◽  
Separation Devices ◽  
Biology Research

Separation is one of the most basic and frequently using techniques for biological research. Researchers have been using gel-electrophoresis for DNA separation and also using various chromatography techniques for protein and bio-molecule separations. Recently, as micro and nano fabrication techniques have developed, interest in miniaturized micro scale biology research tools has also increased. According to this trend, micro scale devices for separating various sized of particles such as cells, organelles, proteins, lipids and vesicles play an important role in a total system. Therefore, separation devices based on various methods are suggested. Widely used separation methods for micro devices are electro-kinetics with special channel geometries and laminar flow control. In electro-kinetic methods, micro channel electrophoresis and DEP (dielectrophoresis) are commonly used.[1] These separation methods, however, can only be used in very narrow range because their working conditions, high voltage and charge dependence, are not compatible with many biomaterials.

scholarly journals In vivo crystallography at X-ray free-electron lasers: the next generation of structural biology?

2014 ◽  
Vol 369 (1647) ◽  
pp. 20130497 ◽  
Author(s):  
François-Xavier Gallat ◽  
Naohiro Matsugaki ◽  
Nathan P. Coussens ◽  
Koichiro J. Yagi ◽  
Marion Boudes ◽  
...  
Keyword(s):  
Structural Biology ◽  
Free Electron ◽  
Free Electron Laser ◽  
Protein Crystal ◽  
X Ray ◽  
Sample Characterization ◽  
The One ◽  
Serial Femtosecond Crystallography

The serendipitous discovery of the spontaneous growth of protein crystals inside cells has opened the field of crystallography to chemically unmodified samples directly available from their natural environment. On the one hand, through in vivo crystallography, protocols for protein crystal preparation can be highly simplified, although the technique suffers from difficulties in sampling, particularly in the extraction of the crystals from the cells partly due to their small sizes. On the other hand, the extremely intense X-ray pulses emerging from X-ray free-electron laser (XFEL) sources, along with the appearance of serial femtosecond crystallography (SFX) is a milestone for radiation damage-free protein structural studies but requires micrometre-size crystals. The combination of SFX with in vivo crystallography has the potential to boost the applicability of these techniques, eventually bringing the field to the point where in vitro sample manipulations will no longer be required, and direct imaging of the crystals from within the cells will be achievable. To fully appreciate the diverse aspects of sample characterization, handling and analysis, SFX experiments at the Japanese SPring-8 angstrom compact free-electron laser were scheduled on various types of in vivo grown crystals. The first experiments have demonstrated the feasibility of the approach and suggest that future in vivo crystallography applications at XFELs will be another alternative to nano-crystallography.

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