Growth orientations, rather than heterogeneous growth rates, dominate jaw joint morphogenesis in the larval zebrafish

In early limb embryogenesis, synovial joints acquire specific shapes which determine joint motion and function. The process by which the opposing cartilaginous joint surfaces are moulded into reciprocal and interlocking shapes, called joint morphogenesis, is one of the least understood aspect of joint formation and the cell-level dynamics underlying it are yet to be unravelled. In this research, we quantified key cellular dynamics involved in growth and morphogenesis of the zebrafish jaw joint and synthesised them in a predictive computational simulation of joint development. Cells in larval zebrafish jaw joints labelled with cartilage markers were tracked over a forty-eight hour time window using confocal imaging. Changes in distance and angle between adjacent cell centroids resulting from cell rearrangement, volume expansion and extracellular matrix (ECM) deposition were measured and used to calculate the rate and direction of local tissue deformations. We observed spatially and temporally heterogeneous growth patterns with marked anisotropy over the developmental period assessed. There was notably elevated growth at the level of the retroarticular process of the Meckel's cartilage, a feature known to undergo pronounced shape changes during zebrafish development. Analysis of cell dynamics indicated a dominant role for cell volume expansion in growth, with minor influences from ECM volume increases and cell intercalation. Cell proliferation in the joint was minimal over the timeframe of interest. Synthesising the dynamic cell data into a finite element model of jaw joint development resulted in accurate shape predictions. Our biofidelic computational simulation demonstrated that zebrafish jaw joint growth can be reasonably approximated based on cell positional information over time, where cell positional information derives mainly from cell orientation and cell volume expansion. By modifying the input parameters of the simulation, we were able to assess the relative contributions of heterogeneous growth rates and of growth orientation. The use of uniform rather than heterogeneous growth rates only minorly impacted the shape predictions whereas isotropic growth fields resulted in altered shape predictions. The simulation results suggest that growth anisotropy is the dominant influence on joint growth and morphogenesis. This study addresses the gap of the cellular processes underlying joint morphogenesis, with implications for understanding the aetiology of developmental joint disorders such as developmental dysplasia of the hip and arthrogryposis.

Interpreting the slope of a straight line in kinematics graphs with school students

The interdisciplinary nature of knowledge is one of the objectives promoted in Colombia, fostering in students the ability to recognize that the curriculum does not work in isolation; if knowledge is complementary, its joint development leads to better academic performance. This research is oriented towards showing an application of the mathematical concept of the slope of the straight line in physics applications, favoring the understanding of the relationships between variables, as occurs with the graphs of kinematics. A group of eleventh grade students from a private educational institution filled in a questionnaire for data collection, based on three situations proposed to answer three open questions in which a reasonably justified answer was required. A part of the group correctly interpreted the graphs by describing the characteristics of the movement in each time interval, but only a few were able to associate the slope of the line as the velocity or acceleration as a function of the variables mentioned in the graph, although their argumentation was based on the angle of elevation of the line.

THE EXPERIENCE OF DIGITALIZATION PROCESS IN RUSSIA AND KAZAKHSTAN IN THE CONTEXT OF BILATERAL COOPERATION

The article examines the global tendency of digitalization as a driving force for the development of the modern world economy. The introduction and development of advanced digital technologies in all spheres of public life in Kazakhstan is one of the main priority areas of state policy. The authors identified similarities and differences in digital transformation through comparative analysis by comprehending the bilateral experience of digitalization between Russia and Kazakhstan. For this purpose, an analysis of the results achieved, strategic programs and documents on the application and development of digital technologies between the two countries was carried out. Also, a review of the features of the formation of public opinion regarding the joint development of the digitalization sphere was carried out.

Research on the Joint Development of Cities of Guangdong-Hong Kong-Macau Greater Bay Area: A Case Study of Foshan City

Promoting the construction of Guangdong-Hong Kong-Macau Greater Bay Area is a major policy decision and national strategy made by the central government of China, which significantly supports the Belt and Road Initiative and maintains the long-term prosperity for the whole country. As an important part of the Guangdong-Hong Kong-Macau Greater Bay Area, Foshan needs to find its own position and direction in order to accelerate the joint development with other cities through five measures.

TEACHING COMMUNICATION SKILLS TO MEDICAL STUDENTS

In connection with the necessity of formation of communicative skills of the future doctor there is an actual question on methodology of formation of skills of the future doctor with patients, namely: what experts (teachers of what departments), when and how should form communicative skills of the future doctor. The purpose of this study is to substantiate the most effective forms and methods of the educational process in the formation of communication skills of the future doctor. 87 % of teachers of clinical departments who participated in the study believe that it is necessary to teach communication skills to medical students from the 1st year of medical school. The most effective they recognize the joint training within the modular discipline using the "standardized patient" method, which, in turn, requires the joint development of clinical scenarios, checklists and other methodological materials to create a database of "clinical cases" for each medical specialty.

Chasing the city that cannot stop: Exploring transportation and urban co-development in São Paulo’s history

We present a historical analysis of transportation and urban development in São Paulo (Brazil), attempting to discern Granger causal effects using historical land-use and transportation data from 1881 to 2013. Our results align with the hypothesis commonly stated in the literature about the relevance of road transportation in São Paulo’s peripheral urban expansion during the twentieth century. We find, however, more complex relationships, and changes in them, over time. Over the entire 130 years, we find that urban expansion and road development pushed and pulled each other, in a somewhat “orderly” way. On the other hand, while roads are not linked to densification, we find that mass transit infrastructure did lead to building densification. Distinguishing among distinct periods adds further insights. Examining São Paulo’s “streetcar era” we find joint development of streetcar lines and urban expansion – evidence of joint development consistent with “streetcar suburbs.” Streetcars also led to building densification during this early period. In subsequent decades, up until the mid-1970s, mass transit investments are virtually non-existent and road transportation essentially chases urban expansion, not vice versa. Finally, the last four decades reveal a return to “orderly” patterns of road expansion and urbanization but no evidence of mass transit infrastructure’s effects on urbanization or densification. The analysis illustrates how transportation investment choices have important consequences for urban growth, exerting long-lasting influences on its urban form.

Review of the monographic study «Corruption-related offenses: administrative legal grounds of counteraction» by Doctor of Legal Sciences Vasyl Franchuk and Doctor of Legal Sciences Dominika Daria Korecka-Szukiewicz (Warsaw: Publishing House «Difin»)

This monograph is determined that corruption as a phenomenon that is a natural result of the joint development and formation of both Ukraine and world community in general is one of the main criteria for distinguishing corruption offenses from corruption-related offenses, because signs of corruption-related offenses are as follows: no signs of corruption; actions violate requirements, prohibitions and restrictions established by the Law of Ukraine «On Prevention of Corruption»; committed by a person specified in Art. 3 of the Law of Ukraine «On Prevention of Corruption»; following legal liability is provided: criminal, administrative, disciplinary and/or civil. Specific proposals to address the most problematic issues of administrative liability for corruption-related offenses, which found their objectification in conclusions to the work and proposals to improve administrative and legal, as well as within certain criminal legal regulation of special confiscation in the legislation of Ukraine were formulated based on what was discovered during the study.