Effect of the Gradient Magnetic Field Stimulation on Extracellular Matrix Synthesis of Chondrocyte

2016 ◽  
Author(s):  
T. Saito ◽  
Y. Morita ◽  
E. Nakamachi
Keyword(s):  
Magnetic Field ◽  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Second Harmonic ◽  
Control Group ◽  
Field Stimulation ◽  
Matrix Structure ◽  
Matrix Synthesis ◽  
Gradient Magnetic Field ◽  
Physical Stimulation

Many researchers tried to improve mechanical properties of the cultured cartilage by physical stimulation. However, its mechanical properties were insufficient. Extracellular matrix structure similar to natural articular cartilage is necessary to form mechanical properties of cultured cartilage. Gradient magnetic field was expected to change pericellular microenvironment associated with local chondrocyte activity. We investigated the effects of gradient magnetic field on chondrocyte activity and extracellular matrix structure. Chondrocytes were cultured under the gradient magnetic field for 7 days, and stimulation time was 12 hours in a day. Intensity of magnetic field was 1.5 mT in the center of a dish. Intensity of gradient magnetic field was 0.03 mT/mm. Chondrocytes were cultured under the uniform magnetic field for control group. The synthesized extracellular matrix in a dish was observed with second harmonic generation, and GAG amount was measured after cultivation. GAG amount in the gradient group was almost same with that in the uniform group. According to SHG image, the area and the thickness of extracellular matrix increased with increasing intensity of magnetic field in the gradient group. This study showed that the gradient magnetic field stimulation formed difference in extracellular matrix structure which depends on local chondrocyte activity.

scholarly journals Reactivating the extracellular matrix synthesis of sulfated glycosaminoglycans and proteoglycans to improve the human skin aspect and its mechanical properties

2016 ◽  
Vol Volume 9 ◽  
pp. 461-472 ◽  
Author(s):  
Hanane Chajra ◽  
Daniel Auriol ◽  
Francine Joly ◽  
Aurélie Pagnon ◽  
Magda Rodrigues ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Human Skin ◽  
Matrix Synthesis

Abstract 1604: Improved Cardiac Function after Expression of the Human Elastin Gene in the Infarcted Myocardium

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Shu-Hong Li ◽  
Zhuo Sun ◽  
Lily Guo ◽  
Mihan Han ◽  
Richard D Weisel ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cardiac Function ◽  
Scar Tissue ◽  
Control Group ◽  
Coronary Artery Ligation ◽  
Stroke Work ◽  
Matrix Structure ◽  
Over Expression ◽  
Infarct Area ◽  
Cardiac Dilatation

Rationale: After a myocardial infarction, necrosed cardiomyocytes are replaced by fibrotic tissue. Subsequent thinning and expansion of this non-elastic scar contributes to cardiac dilatation and congestive heart failure. We attempted to restore the elastic properties of the scar by implanting the infarct area with cells expressing elastin. Methods: Full-length elastin cDNA was generated by PCR using the human lung cDNA library, and then transfected (in adenoviral vector) into rat bone marrow stromal cells (BMSCs). Western blot and immuno-staining confirmed expression of elastin protein. Seven days after coronary artery ligation, BMSCs transfected with genes for either adeno-elastin (BMSC+Elastin) or adeno-green fluorescence protein (BMSC), or media (control group) were injected into the infarct area of adult rats (n=8/group). Results: Over 8 weeks following cell or media implantation, cardiac function (fractional shortening by echocardiography), ventricular volumes, and load independent indices of cardiac function (end systolic and diastolic volumes, preload recruitable stroke work and end systolic elastance by pressure-volume catheter), were all significantly better preserved in both BMSC groups (p<0.05 vs. media), with further improvements in the BMSC+Elastin group (p<0.05 vs. BMSC group). Biochemical analysis detected over-expression of recombinant elastin, and histological examination revealed that collagen fiber length and diameter were preserved in the scar tissue. Myocardial birefringence determined by polarized light (linear retardance of the tissue structure) was significantly highest in the infarct area of the BMSC+Elastin group (p<0.05 compared to BMSC and media groups). Elastin over-expression by cell-based gene therapy restored the elastic matrix structure in the scar tissue, stabilized the infarct and prevented ventricular dilatation, producing the smallest (p<0.05) scar size and surface area in the BMSC+Elastin group. Conclusions: Over-expression of elastin in transplanted BMSCs contributed to the alignment and organization of an elastic extracellular matrix structure, which prevented cardiac dilatation and dysfunction. Elastin plays a key role in the remodeling of the extracellular matrix.

Electric field stimulation through a substrate influences Schwann cell and extracellular matrix structure

2013 ◽  
Vol 10 (4) ◽  
pp. 046011 ◽  
Author(s):  
Hieu T Nguyen ◽  
Claudia Wei ◽  
Jacqueline K Chow ◽  
Lindsey Nguy ◽  
Hieu K Nguyen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Schwann Cell ◽  
Electric Field ◽  
Field Stimulation ◽  
Matrix Structure ◽  
Electric Field Stimulation

Giant Magnetic Field Induced Effects in the Second-Harmonic Generation in a Planar Anisotropic Ta/Co/Pt Structure

JETP Letters ◽  
2020 ◽  
Vol 111 (6) ◽  
pp. 333-337
Author(s):  
T. V. Murzina ◽  
I. A. Kolmychek ◽  
N. S. Gusev ◽  
A. I. Maidykovskii
Keyword(s):  
Magnetic Field ◽  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic

scholarly journals Influence of magnetic field taper on second harmonic 394GHz gyrotron oscillator

AIP Advances ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 015126
Author(s):  
Xuewei Wang ◽  
Qianzhong Xue ◽  
Shan Zhang ◽  
Guohui Zhao ◽  
Gaofeng Liu ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Second Harmonic ◽  
Gyrotron Oscillator

scholarly journals Preparation of Electric- and Magnetic-Activated Water and Its Influence on the Workability and Mechanical Properties of Cement Mortar

2021 ◽  
Vol 13 (8) ◽  
pp. 4546
Author(s):  
Kaiyue Zhao ◽  
Peng Zhang ◽  
Bing Wang ◽  
Yupeng Tian ◽  
Shanbin Xue ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Mechanical Properties ◽  
Compressive Strength ◽  
Flexural Strength ◽  
Cement Paste ◽  
Cement Mortar ◽  
Environmental Issues ◽  
Chemical Admixtures ◽  
Untreated Water ◽  
Alternating Voltage

Cement-based materials prepared with activated water induced by a magnetic field or electric field represent a possible solution to environmental issues caused by the worldwide utilization of chemical admixtures. In this contribution, electric- and magnetic-activated water have been produced. The workability and mechanical properties of cement mortar prepared with this activated water have been investigated. The results indicate that the pH and absorbance (Abs) values of the water varied as the electric and magnetic field changed, and their values increased significantly, exhibiting improved activity compared with that of the untreated water. In addition, activated water still retains activity within 30 min of the resting time. The fluidity of the cement paste prepared with electric-activated water was significantly larger than that of the untreated paste. However, the level of improvement differed with the worst performance resulting from cement paste prepared with alternating voltage activated water. In terms of mechanical properties, both compressive strength and flexural strength obtained its maximum values at 280 mT with two processing cycles. The compressive strength increased 26% as the curing time increased from 7 days to 28 days and flexural strength increased by 31%. In addition, through the introduction of magnetic-activated water into cement mortar, the mechanical strength can be maintained without losing its workability when the amount of cement is reduced.

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