Factors Influencing the Design and Construction of LNG Storage Tanks

Author(s):  
Pascinthe Saad

A number of LNG storage tank designs have been implemented on past and current projects. Such designs include full containment tanks with a 9%Ni inner liner for primary containment and storage of LNG at cryogenic temperature, a carbon steel liner for secondary liquid containment, thermal corner protection and vapor barriers on the inner tank and a concrete outer tank for resistance to external loads. LNG storage tanks are a key component of an LNG liquefaction or regasification facility and there is only a handful of specialized contractors that possess the tank technology and track record of the materials involved and the construction know how required for successful execution. This paper will focus on factors influencing LNG storage tanks design selection and construction execution strategies which can be a function of functional requirements as well as project location.

2008 ◽  
Vol 8 (4) ◽  
pp. 421-426
Author(s):  
J. Menaia ◽  
M. Benoliel ◽  
A. Lopes ◽  
C. Neto ◽  
E. Ferreira ◽  
...  

Concerns arise from the possible occurrence of pathogens in drinking water pipe biofilms and storage tank sediments. In these studies, biofilm samples from pipes and sediments from storage tanks of the Lisbon drinking water distribution system were analyzed. Protein determinations and heterotrophic counts on pipe biofilm samples were used to assess the Lisbon network sessile colonization intensity and distribution. Indicator and pathogenic microorganisms were analyzed in pipe biofilm samples, as well as in storage tanks biofilm and sediments, by using cultural methods and PCR, to assess risks. Results have shown that the Lisbon network sessile colonization is relatively weak in intensity. In addition, no meaningful hazards were apparent for both the network biofilm and the storage tanks biofilm and sediments.


1998 ◽  
Vol 44 (3) ◽  
pp. 571-577 ◽  
Author(s):  
Lynley K Lewis ◽  
Mary W Smith ◽  
Timothy G Yandle ◽  
A Mark Richards ◽  
M Gary Nicholls

Abstract We describe a specific and sensitive RIA for human adrenomedullin (AM)(1–52). The detection limit and the concentration required for 50% inhibition of binding were 0.1 and 1.2 fmol/tube, respectively. Cross-reactivities with AM(1–12), AM(13–52), calcitonin gene-related peptide, amylin, and other vasoactive hormones were negligible. AM immunoreactivity in normal subjects ranged from 2.7 to 10.1 pmol/L (n = 44). We investigated factors influencing the recovery and measurement of AM in the assay. Recovery of labeled AM (>80%) was markedly higher than that of unlabeled AM (56%). Immunoreactivity of exogenous AM added to plasma decreased up to 70% over four freeze–thaw cycles, whereas endogenous AM was stable. Alkali-treated casein (1 g/L) reduced adsorption of AM to surfaces and significantly increased assay precision compared with bovine serum albumin (P <0.0001). HPLC separation of extracted plasma verified the presence of AM(1–52). We suggest that considerable care is needed to ensure that accurate and reproducible results are obtained from studies quantifying this peptide.


Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2091 ◽  
Author(s):  
Yue Hu ◽  
Zhou Chen ◽  
Zheng Hou ◽  
Mingkai Li ◽  
Bo Ma ◽  
...  

The DNA tetrahedron (Td), as one of the novel DNA-based nanoscale biomaterials, has been extensively studied because of its excellent biocompatibility and increased possibilities for decorating precisely. Although the use of Td in laboratories is well established, knowledge surrounding the factors influencing its preparation and storage is lacking. In this research, we investigated the role of the magnesium ions, which greatly affect the structure and stability of DNA. We assembled 1, 2, 5, 10 and 20 μM Td in buffers containing different Mg2+ concentrations, demonstrating that 2 and 5 mM Mg2+ is optimal in these conditions, and that yields decrease dramatically once the DNA concentration reaches 20 μM or the Mg2+ concentration is lower than 0.5 mM. We also verified that the Td structure is retained better through freeze-thawing than lyophilization. Furthermore, a lower initial Mg2+ (≤2 mM) benefited the maintenance of Td structure in the process of lyophilization. Hence, our research sheds light on the influence of Mg2+ in the process of preparing and storing Td, and also provides some enlightenment on improving yields of other DNA nanostructures.


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