Electrospinning of Recombinant Protein Polymer Nanofibers

2009 ◽  
Author(s):  
Weiguo Qiu ◽  
Arjun Stokes ◽  
Joseph Cappello ◽  
Xiaoyi Wu
Keyword(s):  
Thin Films ◽  
Recombinant Proteins ◽  
Molecular Design ◽  
New Technology ◽  
Building Blocks ◽  
Structure And Function ◽  
Structural Proteins ◽  
Protein Polymer ◽  
Nanofibrous Scaffolds ◽  
And Function

Structural proteins often in the form of micro and nanofibers, constituting most of intra- and extracellular matrix (ECM), are the fundamental building blocks of life [1]. Recent efforts to replace diseased or damaged tissues and organs have resulted in the molecular design and genetic engineering of recombinant proteins, and the advent of new technology for fabricating structural proteins into micro-/nanofibrous scaffolds, hoping to resemble some or all the characteristics of ECM structure and function. The fabrication of such an ECM mimic may be an important step in engineering a functional tissue. To this end, we have produced a series of silk-elastin-like proteins (SELPs) [2]. Revealed by our subsequent studies, SELPs in the form of hydrogels, thin films, and microfibers, have displayed a set of outstanding biological and physical properties. In this study, electrospinning will be pursued as a mechanism for the formation of SELP nanofibers.

scholarly journals Engineering meniscus structure and function via multi-layered mesenchymal stem cell-seeded nanofibrous scaffolds

2015 ◽  
Vol 48 (8) ◽  
pp. 1412-1419 ◽  
Author(s):  
Matthew B. Fisher ◽  
Elizabeth A. Henning ◽  
Nicole Söegaard ◽  
Marc Bostrom ◽  
John L. Esterhai ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Structure And Function ◽  
Nanofibrous Scaffolds ◽  
And Function

scholarly journals Structure and Function of Multimeric G-Quadruplexes

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3074 ◽  
Author(s):  
Sofia Kolesnikova ◽  
Edward A. Curtis
Keyword(s):  
Nucleic Acid ◽  
Building Blocks ◽  
Structure And Function ◽  
Limited Information ◽  
Functional Elements ◽  
Future Studies ◽  
And Function ◽  
Nucleic Acid Structures ◽  
Sequence Requirements ◽  
Order Structures

G-quadruplexes are noncanonical nucleic acid structures formed from stacked guanine tetrads. They are frequently used as building blocks and functional elements in fields such as synthetic biology and also thought to play widespread biological roles. G-quadruplexes are often studied as monomers, but can also form a variety of higher-order structures. This increases the structural and functional diversity of G-quadruplexes, and recent evidence suggests that it could also be biologically important. In this review, we describe the types of multimeric topologies adopted by G-quadruplexes and highlight what is known about their sequence requirements. We also summarize the limited information available about potential biological roles of multimeric G-quadruplexes and suggest new approaches that could facilitate future studies of these structures.

scholarly journals Rotavirus non-structural proteins: structure and function

2012 ◽  
Vol 2 (4) ◽  
pp. 380-388 ◽  
Author(s):  
Liya Hu ◽  
Sue E Crawford ◽  
Joseph M Hyser ◽  
Mary K Estes ◽  
BV Venkataram Prasad
Keyword(s):  
Structure And Function ◽  
Structural Proteins ◽  
And Function

scholarly journals Changed reactivity of secondary hydroxyl groups in C8-modified adenosine – lessons learned from silylation

2020 ◽  
Author(s):  
Jennifer Frommer ◽  
Sabine Müller
Keyword(s):  
Building Block ◽  
Rna Structure ◽  
Building Blocks ◽  
Structure And Function ◽  
Lessons Learned ◽  
Hydroxyl Groups ◽  
Modified Oligonucleotides ◽  
Secondary Hydroxyl ◽  
Protection Scheme ◽  
And Function

Synthesis of site-specifically modified oligonucleotides has become a major tool for RNA structure and function studies. Reporter groups or specific functional entities are required to be attached at a pre-defined site of the oligomer.  An attractive strategy is the incorporation of suitably functionalized building blocks that allow post-synthetic conjugation of the desired moiety. A C8-alkynyl modified adenosine derivative was synthesized, reviving an old synthetic pathway for iodination of purine nucleobases. Silylation of the C8-alkynyl modified adenosine revealed unexpected selectivity of the two secondary sugar hydroxyl groups, with the 3'-O-isomer being preferentially formed. Optimization of the protection scheme lead to a new and economic route to the desired C8-alkynylated building block and its incorporation in RNA.

scholarly journals Isolation of the Buchnera aphidicola flagellum basal body complexes from the Buchnera membrane

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0245710
Author(s):  
Matthew J. Schepers ◽  
James N. Yelland ◽  
Nancy A. Moran ◽  
David W. Taylor
Keyword(s):  
Basal Body ◽  
Gene Clusters ◽  
Cellular Membrane ◽  
Structure And Function ◽  
Bacterial Symbiont ◽  
Structural Proteins ◽  
Buchnera Aphidicola ◽  
Large Numbers ◽  
And Function ◽  
Aphid Host

Buchnera aphidicola is an intracellular bacterial symbiont of aphids and maintains a small genome of only 600 kbps. Buchnera is thought to maintain only genes relevant to the symbiosis with its aphid host. Curiously, the Buchnera genome contains gene clusters coding for flagellum basal body structural proteins and for flagellum type III export machinery. These structures have been shown to be highly expressed and present in large numbers on Buchnera cells. No recognizable pathogenicity factors or secreted proteins have been identified in the Buchnera genome, and the relevance of this protein complex to the symbiosis is unknown. Here, we show isolation of Buchnera flagellum basal body proteins from the cellular membrane of Buchnera, confirming the enrichment of flagellum basal body proteins relative to other proteins in the Buchnera proteome. This will facilitate studies of the structure and function of the Buchnera flagellum structure, and its role in this model symbiosis.

scholarly journals Changed reactivity of secondary hydroxy groups in C8-modified adenosine – lessons learned from silylation

2020 ◽  
Vol 16 ◽  
pp. 2854-2861
Author(s):  
Jennifer Frommer ◽  
Sabine Müller
Keyword(s):  
Building Block ◽  
Rna Structure ◽  
Building Blocks ◽  
Structure And Function ◽  
Lessons Learned ◽  
Modified Oligonucleotides ◽  
Synthetic Pathway ◽  
Protection Scheme ◽  
And Function ◽  
Hydroxy Groups

Synthesis of site-specifically modified oligonucleotides has become a major tool for RNA structure and function studies. Reporter groups or specific functional entities are required to be attached at a pre-defined site of the oligomer. An attractive strategy is the incorporation of suitably functionalized building blocks that allow post-synthetic conjugation of the desired moiety. A C8-alkynyl-modified adenosine derivative was synthesized, reviving an old synthetic pathway for iodination of purine nucleobases. Silylation of the C8-alkynyl-modified adenosine revealed unexpected selectivity of the two secondary sugar hydroxy groups, with the 3'-O-isomer being preferentially formed. Optimization of the protection scheme lead to a new and economic route to the desired C8-alkynylated building block and its incorporation in RNA.

scholarly journals Beyond Expectation: Advanced Materials Design, Synthesis, and Processing to Enable Novel Ferroelectric Properties and Applications

MRS Advances ◽  
2020 ◽  
Vol 5 (64) ◽  
pp. 3453-3472
Author(s):  
Jieun Kim ◽  
Eduardo Lupi ◽  
David Pesquera ◽  
Megha Acharya ◽  
Wenbo Zhao ◽  
...  
Keyword(s):  
Thin Films ◽  
Computational Modeling ◽  
Ferroelectric Properties ◽  
Structure And Function ◽  
Advanced Materials ◽  
Design Synthesis ◽  
Fundamental Understanding ◽  
Synthesis And Processing ◽  
Emergent Structure ◽  
And Function

AbstractFerroelectrics and related materials (e.g., non-traditional ferroelectrics such as relaxors) have long been used in a range of applications, but with the advent of new ways of modeling, synthesizing, and characterizing these materials, continued access to astonishing breakthroughs in our fundamental understanding come each year. While we still rely on these materials in a range of applications, we continue to re-write what is possible to be done with them. In turn, assumptions that have underpinned the use and design of certain materials are progressively being revisited. This perspective aims to provide an overview of the field of ferroelectric/relaxor/polar-oxide thin films in recent years, with an emphasis on emergent structure and function enabled by advanced synthesis, processing, and computational modeling.

Building ZOOB Models to Help Students Learn about Nucleic Acid Structure and Function

2017 ◽  
Vol 79 (4) ◽  
pp. 294-300
Author(s):  
Elizabeth A. Mulligan
Keyword(s):  
Nucleic Acid ◽  
Deductive Reasoning ◽  
Building Blocks ◽  
Structure And Function ◽  
Nucleic Acid Structure ◽  
Simplified Models ◽  
Collaborative Activity ◽  
Dna And Rna ◽  
And Function ◽  
Acid Structure

This paper describes a collaborative activity for students, which allows them to build simplified models of individual nucleotides, DNA, and RNA using ZOOB building blocks. These models help students learn about nucleic acid structure and the process of transcription. In addition, students learn how to work in groups as well as practice critical thinking and deductive reasoning while building these models.

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