scholarly journals Basic Study of Beutin's Washed Sheep Blood Agar Plate Used for Selective Screening of Verocytotoxin-producing/Enterohemorrhagic Escherichia coli (VTEC/EHEC)

1999 ◽  
Vol 73 (4) ◽  
pp. 318-327 ◽  
Author(s):  
Nobuaki KIMURA ◽  
Akiko KOZAKI ◽  
Tomiko SASAKI ◽  
Akira KOMATSUBARA
Keyword(s):  
Escherichia Coli ◽  
Agar Plate ◽  
Enterohemorrhagic Escherichia Coli ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Selective Screening ◽  
Basic Study ◽  
Sheep Blood ◽  
Sheep Blood Agar Plate

scholarly journals Gentamicin-blood agar for isolation of Streptococcus pneumoniae from respiratory secretions

1978 ◽  
Vol 7 (5) ◽  
pp. 426-427
Author(s):  
R E Schmid ◽  
J A Washington ◽  
J P Anhalt
Keyword(s):  
Streptococcus Pneumoniae ◽  
Primary Culture ◽  
Agar Plate ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Minimal Inhibitory Concentrations ◽  
Sheep Blood ◽  
Sheep Blood Agar Plate ◽  
Respiratory Specimens

Previous studies have suggested that the yield of Streptococcus pneumoniae from respiratory secretions can be increased by using a 5% sheep blood agar plate supplemented with 5 microgram of gentamicin (GBA) per ml. We report our experience with 245 lower respiratory specimens in which this method was compared with 5% sheep blood agar (SBA) alone. Of 35 specimens with growth of S. pneumoniae on either plate, 21 were detected exclusively on SBA, whereas only 3 were detected on GBA alone (P less than 0.01). By subculturing representative alpha-hemolytic colonies from the final 169 specimens, the yield of S. pneumoniae was increased by 27% compared with the number of identifications that could be made directly from the primary culture. Minimal inhibitory concentrations of gentamicin for the last 25 isolates were greater than or equal to 8 microgram/ml. Our results do not substantiate the previous observations that S. pneumoniae from respiratory secretions gives an increased yield in cultures on GBA.

scholarly journals Modified coagglutination procedure for the serological grouping of streptococci

1979 ◽  
Vol 9 (3) ◽  
pp. 329-332
Author(s):  
J R Carlson ◽  
L R McCarthy
Keyword(s):  
Agar Plate ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Reliable Technique ◽  
Cross Reactions ◽  
Sheep Blood ◽  
Plate Assay ◽  
Sheep Blood Agar Plate ◽  
Streptomyces Albus

Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the supernatant was tested by slide coagglutination. Positive coagglutination reactions occurred within 30 s. The cell pellets from overnight broth cultures and colonies taken directly from sheep blood agar plates were tested and compared with the results of the Lancefield capillary precipitin method. Of the 102 strains of broth-grown cells tested, 100 were grouped by the MCAP and the Lancefield capillary precipitin method. The remaining two isolates were serologically identified only by the MCAP. Of the original 102 strains, 97 were tested by MCAP after extraction of five well-isolated colonies from a sheep blood agar plate. When this latter method was used, 95.9% of the strains were correctly identified. Nonspecific reactions were observed only while testing the MCAP with the direct plate assay. These cross-reactions were remedied promptly by either absorption or dilution of the antisera involved. The MCAP was found to be a rapid and reliable technique for the serological grouping of streptococci.

scholarly journals Improved reliability of the primary plate bacitracin test on throat cultures with sulfamethoxazole-trimethoprim blood agar plates

1979 ◽  
Vol 9 (1) ◽  
pp. 144-146 ◽  
Author(s):  
T Kurzynski ◽  
C Meise ◽  
R Daggs ◽  
A Helstad
Keyword(s):  
Agar Plate ◽  
Susceptibility Test ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Group A Streptococci ◽  
Sheep Blood Agar Plate ◽  
Group A ◽  
Primary Plate ◽  
Disk Susceptibility

The primary plate bacitracin differentiation disk susceptibility test identified 85% of group A streptococci from throat cultures on SXT-BA(CO2) plates within 24 h, as compared to only 26% on a conventional sheep blood agar plate.

Characterization and antibiogram of enteropathogenic Escherichia coli isolated from diarrhoeic and non-diarrhoeic dogs in South Bengal, India

2015 ◽  
Author(s):  
A. Banik ◽  
D. P. Isore ◽  
S. N. Joardar ◽  
K. Batabyal ◽  
S. Dey
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
West Bengal ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Enteropathogenic Escherichia Coli ◽  
Highly Pathogenic ◽  
E Coli ◽  
Sheep Blood ◽  
Common Serotypes

Diarrhoea in canines is mainly caused by Escherichia coli which can be fatal also. To understand the depth of this infection, a study was undertaken to detect E. coli isolates from diarrhoeic and non-diarrhoeic dogs in Southern part of West Bengal. A total of 112 canine samples were tested during May to September 2012 revealing approx 63.4% (71) samples positive for Escherichia coli. The most common serotypes were O8 (23.9%) followed by O157 (19.7%), O101 (16.9%), O26 (15.5%), O153 (12.7%) and O6 (11.3%). Among these O6, O8, O26 and O157 were highly pathogenic to mice causing almost 100% mortality within 24hrs of inoculation and were also detected to be haemolytic on sheep blood agar plates except serotype O8. These isolates were mostly sensitive to nalidixic acid (80.29%), cotrimoxazole (78.88%), ciprofloxacin (74.65%), colistin and ceftriaxone (both 71.83%) but were resistant to amikacin (97.18%), kanamycin (95.78%), cephalexin (92.96%) and enrofloxacin (84.51%).

scholarly journals Sterility Duration of Preprimed Extracorporeal Membrane Oxygenation Circuits

2018 ◽  
Vol 23 (4) ◽  
pp. 311-314 ◽  
Author(s):  
Vi Ean Tan ◽  
Alan T. Evangelista ◽  
Dominick M. Carella ◽  
Daniel Marino ◽  
Wayne S. Moore ◽  
...  
Keyword(s):  
Extracorporeal Membrane Oxygenation ◽  
Room Temperature ◽  
Agar Plate ◽  
Fungal Growth ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Macconkey Agar ◽  
Pilot Data ◽  
Sheep Blood ◽  
Membrane Oxygenation

OBJECTIVES There is a lack of standardization and supporting data regarding the duration preassembled and preprimed extracorporeal membrane oxygenation (ECMO) circuits are expected to be sterile. Therefore, the purpose of this study was to prospectively evaluate whether preassembled and preprimed ECMO circuits could maintain sterility for a period up to 65 days. DESIGN Four ECMO circuits (2 neonatal/pediatric¼” and 2 adolescent/adult ⅜ ”) were assembled and primed under sterile conditions and maintained at room temperature. Culture samples were obtained from each circuit and plated within 1 hour. Culture samples were obtained on day 0 when assembled and primed then every 5 days up to day 65. Samples were plated on several different media including the following: blood agar plate: trypticase soy agar with 5% sheep blood, MacConkey agar, and thioglycollate broth then incubated at 35°C for 3 days. RESULTS All cultures obtained from the priming solution from of the¼” and ⅜ ” ECMO circuits produced no microbial or fungal growth for the 65-day study period. CONCLUSION These pilot data suggest preprimed ECMO circuits may maintain sterility for a period up to 65 days. Additional studies evaluating a larger number of ECMO circuits are needed to confirm these findings.

Rapid Streptococcal Tests

PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 288-288
Author(s):  
S. DUBOSE RAVENEL ◽  
GREGORY CARL ELLIS ◽  
WILLIAM N. MICHAL
Keyword(s):  
Blood Culture ◽  
Sensitivity And Specificity ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Practice Setting ◽  
Throat Culture ◽  
Culture Techniques ◽  
Sheep Blood ◽  
Office Practice ◽  
Important Study

Roddey et al have reported an important study on the sensitivity and specificity of the Strep A OIA test compared with two culture techniques—5% sheep blood agar and Todd-Hewitt broth—in an office practice setting. They found the sensitivity and specificity of OIA as compared with sheep blood culture to be 91.4% and 95.6%, and compared with the broth method, 90.4% and 94.1%, respectively. They conclude that the OIA method is preferable for the majority of their patients, but recommend a throat culture be performed in cases with a negative OIA test.

Sign in / Sign up

Export Citation Format

Share Document