scholarly journals Gentamicin-blood agar for isolation of Streptococcus pneumoniae from respiratory secretions

1978 ◽  
Vol 7 (5) ◽  
pp. 426-427
Author(s):  
R E Schmid ◽  
J A Washington ◽  
J P Anhalt
Keyword(s):  
Streptococcus Pneumoniae ◽  
Primary Culture ◽  
Agar Plate ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Minimal Inhibitory Concentrations ◽  
Sheep Blood ◽  
Sheep Blood Agar Plate ◽  
Respiratory Specimens

Previous studies have suggested that the yield of Streptococcus pneumoniae from respiratory secretions can be increased by using a 5% sheep blood agar plate supplemented with 5 microgram of gentamicin (GBA) per ml. We report our experience with 245 lower respiratory specimens in which this method was compared with 5% sheep blood agar (SBA) alone. Of 35 specimens with growth of S. pneumoniae on either plate, 21 were detected exclusively on SBA, whereas only 3 were detected on GBA alone (P less than 0.01). By subculturing representative alpha-hemolytic colonies from the final 169 specimens, the yield of S. pneumoniae was increased by 27% compared with the number of identifications that could be made directly from the primary culture. Minimal inhibitory concentrations of gentamicin for the last 25 isolates were greater than or equal to 8 microgram/ml. Our results do not substantiate the previous observations that S. pneumoniae from respiratory secretions gives an increased yield in cultures on GBA.

scholarly journals Modified coagglutination procedure for the serological grouping of streptococci

1979 ◽  
Vol 9 (3) ◽  
pp. 329-332
Author(s):  
J R Carlson ◽  
L R McCarthy
Keyword(s):  
Agar Plate ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Reliable Technique ◽  
Cross Reactions ◽  
Sheep Blood ◽  
Plate Assay ◽  
Sheep Blood Agar Plate ◽  
Streptomyces Albus

Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the supernatant was tested by slide coagglutination. Positive coagglutination reactions occurred within 30 s. The cell pellets from overnight broth cultures and colonies taken directly from sheep blood agar plates were tested and compared with the results of the Lancefield capillary precipitin method. Of the 102 strains of broth-grown cells tested, 100 were grouped by the MCAP and the Lancefield capillary precipitin method. The remaining two isolates were serologically identified only by the MCAP. Of the original 102 strains, 97 were tested by MCAP after extraction of five well-isolated colonies from a sheep blood agar plate. When this latter method was used, 95.9% of the strains were correctly identified. Nonspecific reactions were observed only while testing the MCAP with the direct plate assay. These cross-reactions were remedied promptly by either absorption or dilution of the antisera involved. The MCAP was found to be a rapid and reliable technique for the serological grouping of streptococci.

scholarly journals Improved reliability of the primary plate bacitracin test on throat cultures with sulfamethoxazole-trimethoprim blood agar plates

1979 ◽  
Vol 9 (1) ◽  
pp. 144-146 ◽  
Author(s):  
T Kurzynski ◽  
C Meise ◽  
R Daggs ◽  
A Helstad
Keyword(s):  
Agar Plate ◽  
Susceptibility Test ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Group A Streptococci ◽  
Sheep Blood Agar Plate ◽  
Group A ◽  
Primary Plate ◽  
Disk Susceptibility

The primary plate bacitracin differentiation disk susceptibility test identified 85% of group A streptococci from throat cultures on SXT-BA(CO2) plates within 24 h, as compared to only 26% on a conventional sheep blood agar plate.

scholarly journals Sterility Duration of Preprimed Extracorporeal Membrane Oxygenation Circuits

2018 ◽  
Vol 23 (4) ◽  
pp. 311-314 ◽  
Author(s):  
Vi Ean Tan ◽  
Alan T. Evangelista ◽  
Dominick M. Carella ◽  
Daniel Marino ◽  
Wayne S. Moore ◽  
...  
Keyword(s):  
Extracorporeal Membrane Oxygenation ◽  
Room Temperature ◽  
Agar Plate ◽  
Fungal Growth ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Macconkey Agar ◽  
Pilot Data ◽  
Sheep Blood ◽  
Membrane Oxygenation

OBJECTIVES There is a lack of standardization and supporting data regarding the duration preassembled and preprimed extracorporeal membrane oxygenation (ECMO) circuits are expected to be sterile. Therefore, the purpose of this study was to prospectively evaluate whether preassembled and preprimed ECMO circuits could maintain sterility for a period up to 65 days. DESIGN Four ECMO circuits (2 neonatal/pediatric¼” and 2 adolescent/adult ⅜ ”) were assembled and primed under sterile conditions and maintained at room temperature. Culture samples were obtained from each circuit and plated within 1 hour. Culture samples were obtained on day 0 when assembled and primed then every 5 days up to day 65. Samples were plated on several different media including the following: blood agar plate: trypticase soy agar with 5% sheep blood, MacConkey agar, and thioglycollate broth then incubated at 35°C for 3 days. RESULTS All cultures obtained from the priming solution from of the¼” and ⅜ ” ECMO circuits produced no microbial or fungal growth for the 65-day study period. CONCLUSION These pilot data suggest preprimed ECMO circuits may maintain sterility for a period up to 65 days. Additional studies evaluating a larger number of ECMO circuits are needed to confirm these findings.

scholarly journals Epidemiological studies of Streptococcus pneumoniae in infants: methods of isolating pneumococci

1977 ◽  
Vol 5 (3) ◽  
pp. 293-296
Author(s):  
G M Converse ◽  
H C Dillon
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Infection ◽  
Epidemiological Studies ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Isolation And Identification ◽  
Sheep Blood ◽  
Healthy Infants ◽  
Solid Culture Medium ◽  
A Prospective Study

A prospective study of the natural history of pneumococcal infection, which involves serial culture studies in healthy infants from 6 weeks of age onward, is in progress in our laboratory. This report describes results of a comparison of several methods for the isolation and identification of Streptococcus pneumoniae from the nasopharynges and throats of these infants. Sheep blood agar, sheep blood agar with gentamicin sulfate (gentamicin agar), and mouse inoculation with 4-h broth cultures were used. Gentamicin agar proved superior to plain sheep blood agar as a solid culture medium, especially in enhancing the recovery of pneumococci from throat cultures. With gentamicin agar, similar carrier rates were found for both culture sites (nasopharynx and throat). In addition, gentamicin agar proved superior to mouse inoculation for the recovery of carrier strains from 131 nasopharyngeal culture samples processed by both methods. Sixty of 131 samples were positive for pneumococci, 25% of which would have been missed had mouse inoculation alone been used. In only three instances did we recover a strain by mouse inoculation that failed to grow on gentamicin agar; conversely, 15 strains were isolated on gentamicin agar but could not be recovered from mice. The latter observation might be explained by the fact that certain carrier strains may be relatively mouse avirulent. The use of blood agar containing gentamicin appears to offer a simple and inexpensive method for the recovery of S. pneumoniae and, in our opinion, provides an ideal method for the identification of pneumococcal carriers as well as for the recovery of these strains from clinical material such as sputum or ear exudates, where other and less fastidious organisms may also be present.

Variants of the bile: solubility test to differentiate Streptococcus pneumoniae from other viridans group streptococci

2019 ◽  
Vol 14 (11) ◽  
pp. 949-955
Author(s):  
María Ercibengoa ◽  
María Morales ◽  
Marta Alonso ◽  
Carmen Ardanuy ◽  
José M Marimón
Keyword(s):  
Streptococcus Pneumoniae ◽  
16S Rrna ◽  
Bile Salts ◽  
Agar Plate ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Solubility Test ◽  
Naked Eye ◽  
Direct Testing ◽  
Viridans Group Streptococci

Aim: Bile salts promote the specific autolysis of pneumococcal cells, allowing the differentiation between Streptococcus pneumoniae and other viridans group streptococci (VGS). Material & methods: One hundred clinical VGS isolates identified by amplification and sequencing of 16S rRNA, groEL and sodA genes were analyzed with different variants of bile-solubility tests: tube testing read by naked eye; tube testing where the lysis was measured as the decrease of turbidity with a densitometer; and direct testing on blood agar plate. Results: As expected, all S. pneumoniae isolates were fully lysed in the presence of bile salts except for one isolate that partially lysate in tube testing as well as on the blood agar plate. None of the VGS were lysed by bile salts. Conclusion: Bile-solubility testing is an accurate and technically nondemanding method to discriminate between S. pneumoniae and other VGS species.

scholarly journals Value of sputum culture in diagnosis of pneumococcal pneumonia

1977 ◽  
Vol 6 (1) ◽  
pp. 62-65
Author(s):  
W L Drew
Keyword(s):  
Streptococcus Pneumoniae ◽  
Pneumococcal Pneumonia ◽  
Agar Plate ◽  
Laboratory Culture ◽  
Sputum Culture ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Anaerobic Incubation ◽  
Bacteremic Pneumococcal Pneumonia

In our laboratory, culture of sputum was extremely useful in diagnosing the etiology of pneumococcal pneumonia. Of 31 consecutive patients with bacteremic pneumococcal pneumonia, 29 (94%) had Streptococcus pneumoniae cultured from sputum. Recovery of pneumococci in culture was enhanced by anaerobic incubation as well as by a plate bile test and an optochin disk on a primary blood agar plate.

Rapid Streptococcal Tests

PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 288-288
Author(s):  
S. DUBOSE RAVENEL ◽  
GREGORY CARL ELLIS ◽  
WILLIAM N. MICHAL
Keyword(s):  
Blood Culture ◽  
Sensitivity And Specificity ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Practice Setting ◽  
Throat Culture ◽  
Culture Techniques ◽  
Sheep Blood ◽  
Office Practice ◽  
Important Study

Roddey et al have reported an important study on the sensitivity and specificity of the Strep A OIA test compared with two culture techniques—5% sheep blood agar and Todd-Hewitt broth—in an office practice setting. They found the sensitivity and specificity of OIA as compared with sheep blood culture to be 91.4% and 95.6%, and compared with the broth method, 90.4% and 94.1%, respectively. They conclude that the OIA method is preferable for the majority of their patients, but recommend a throat culture be performed in cases with a negative OIA test.

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