Multi-modal microscopy platform including optical tweezers and the need to perform spherical wave vectors decompositions for optical force and signal intensity calculations (Conference Presentation)

2017 ◽  
Author(s):  
Carlos Lenz Cesar ◽  
Wendel L. Moreira ◽  
Antonio A. R. Neves ◽  
André A. de Thomaz ◽  
Diogo B. Almeida ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Signal Intensity ◽  
Spherical Wave ◽  
Optical Force ◽  
Intensity Calculations

scholarly journals Investigation of the polarization-dependent optical force in optical tweezers by using generalized Lorenz-Mie theory

2015 ◽  
Vol 67 (12) ◽  
pp. 2086-2091 ◽  
Author(s):  
Jai-Min Choi ◽  
Heeso Noh
Keyword(s):  
Optical Tweezers ◽  
Mie Theory ◽  
Optical Force

scholarly journals Micromanipulation of amyloplasts with optical tweezers in Arabidopsis stems

2020 ◽  
Author(s):  
Yoshinori Abe ◽  
Keisuke Meguriya ◽  
Takahisa Matsuzaki ◽  
Teruki Sugiyama ◽  
Hiroshi Y. Yoshikawa ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Near Infrared ◽  
Initial Step ◽  
Optical Force ◽  
Physical Interaction ◽  
Laser Focus ◽  
Close Proximity ◽  
Endodermal Cells ◽  
Vacuolar Membranes

AbstractIntracellular sedimentation of highly dense, starch-filled amyloplasts toward the gravity vector is likely a key initial step for gravity sensing in plants. However, recent live-cell imaging technology revealed that most amyloplasts continuously exhibit dynamic, saltatory movements in the endodermal cells of Arabidopsis stems. These complicated movements led to questions about what type of amyloplast movement triggers gravity sensing. Here we show that a confocal microscope equipped with optical tweezers can be a powerful tool to trap and manipulate amyloplasts noninvasively, while simultaneously observing cellular responses such as vacuolar dynamics in living cells. A near-infrared (λ = 1064 nm) laser that was focused into the endodermal cells at 1 mW of laser power attracted and captured amyloplasts at the laser focus. The optical force exerted on the amyloplasts was theoretically estimated to be up to 1 pN. Interestingly, endosomes and trans-Golgi networks were trapped at 30 mW but not at 1 mW, which is probably due to lower refractive indices of these organelles than that of the amyloplasts. Because amyloplasts are in close proximity to vacuolar membranes in endodermal cells, their physical interaction could be visualized in real time. The vacuolar membranes drastically stretched and deformed in response to the manipulated movements of amyloplasts by optical tweezers. Our new method provides deep insights into the biophysical properties of plant organelles in vivo and opens a new avenue for studying gravity-sensing mechanisms in plants.

TRAPPING OF GOLD NANOPARTICLE AND POLYSTYRENE BEADS BY DYNAMIC OPTICAL TWEEZERS

2015 ◽  
Vol 74 (8) ◽  
Author(s):  
M. S. Aziz ◽  
K. Tufail ◽  
N. E. Khamsan ◽  
S. Affandi ◽  
S. Daud ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Dark Soliton ◽  
Input Power ◽  
Optical Force ◽  
Gradient Force ◽  
Polystyrene Beads ◽  
Multiple Trapping ◽  
Different Diameters ◽  
20 Nm ◽  
Good Agreement

Gold nanoparticles and polystyrene beads are very important to use in advanced nanoscopic optical trapping techniques to probe any biological system of interest. Multiple trapping of these particles with different diameters can be performed by an optical tweezers system employing dark soliton controlled by Gaussian pulse within a particular configuration of microring resonators. By controlling some parameters and input power of the system, dynamics of the tweezers can be tuned. Radiation pressure acting on the particles including gradient and scattering forces were theoretically measured as a function of normalized position from the center of the laser beam. In this work, the highest output signal in the form of potential well is recorded at 112.80 W corresponding to 1.6 mm wavelength. Sizes of the tweezers are found within the range of 20 nm and the highest value of the optical force is recorded at 895.70 pN. We have demonstrated that the gradient force component is dominant over particle size within Rayleigh regime, thus a good agreement with theory is found.

scholarly journals Single-molecule mechanics of protein-labelled DNA handles

2016 ◽  
Vol 7 ◽  
pp. 138-148 ◽  
Author(s):  
Vivek S Jadhav ◽  
Dorothea Brüggemann ◽  
Florian Wruck ◽  
Martin Hegner
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Mechanical Characteristics ◽  
Molecular Motor ◽  
Optical Force ◽  
Depth Information ◽  
Physiological Conditions ◽  
Modified Dna ◽  
Applied Forces ◽  
Surface Modified

DNA handles are often used as spacers and linkers in single-molecule experiments to isolate and tether RNAs, proteins, enzymes and ribozymes, amongst other biomolecules, between surface-modified beads for nanomechanical investigations. Custom DNA handles with varying lengths and chemical end-modifications are readily and reliably synthesized en masse, enabling force spectroscopic measurements with well-defined and long-lasting mechanical characteristics under physiological conditions over a large range of applied forces. Although these chemically tagged DNA handles are widely used, their further individual modification with protein receptors is less common and would allow for additional flexibility in grabbing biomolecules for mechanical measurements. In-depth information on reliable protocols for the synthesis of these DNA–protein hybrids and on their mechanical characteristics under varying physiological conditions are lacking in literature. Here, optical tweezers are used to investigate different protein-labelled DNA handles in a microfluidic environment under different physiological conditions. Digoxigenin (DIG)-dsDNA-biotin handles of varying sizes (1000, 3034 and 4056 bp) were conjugated with streptavidin or neutravidin proteins. The DIG-modified ends of these hybrids were bound to surface-modified polystyrene (anti-DIG) beads. Using different physiological buffers, optical force measurements showed consistent mechanical characteristics with long dissociation times. These protein-modified DNA hybrids were also interconnected in situ with other tethered biotinylated DNA molecules. Electron-multiplying CCD (EMCCD) imaging control experiments revealed that quantum dot–streptavidin conjugates at the end of DNA handles remain freely accessible. The experiments presented here demonstrate that handles produced with our protein–DNA labelling procedure are excellent candidates for grasping single molecules exposing tags suitable for molecular recognition in time-critical molecular motor studies.

The Measurement of Displacement and Optical Force in Multi-Optical Tweezers

2012 ◽  
Vol 29 (1) ◽  
pp. 014214 ◽  
Author(s):  
Lin Ling ◽  
Hong-Lian Guo ◽  
Lu Huang ◽  
E Qu ◽  
Zhao-Lin Li ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Optical Force

scholarly journals Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

2019 ◽  
Vol 29 (3) ◽  
Author(s):  
Ho Quang Quy
Keyword(s):  
Optical Tweezers ◽  
Optical Method ◽  
Nonlinear Optical ◽  
Medical Science ◽  
Optical Force ◽  
Trapping Efficiency ◽  
Optical Efficiency ◽  
Trapping Region ◽  
Common Trend

Optical tweezers have seen as an essential tool for the manipulation dielectric microparticles and nanoparticles due to its non-contact action and high resolution of optical force. Up to now, there has been a lot of optical tweezers applications in the fields of biophysics, chemistry, medical science and nanoscience. Recently, optical tweezers have been theoretically and experimentally developing for the nanomechanical characterization of various kinds of biological cells. The configuration of optical tweezers has been day after day improving to enhance the trapping efficiency, spatial and temporal resolution and easy to control trapped objects. In common trend of optical tweezers improvements, we will discuss in detail of the several configurations of nonlinear optical tweezers using nonlinear materials as the added lens. We will also address the advantages of nonlinear optical tweezers, such as enhance optical efficiency, reduce trapping region, simplify controlling all-optical method. Finally, we present discussions about the specific properties of nonlinear optical tweezers used for stretch DNA molecule as example and an ideal to improve nonlinear  optical tweezers using thin layer of organic dye  proposed for going time.

Optical force sensor array in a microfluidic device based on holographic optical tweezers

Lab on a Chip ◽  
2009 ◽  
Vol 9 (5) ◽  
pp. 661 ◽  
Author(s):  
Kai Uhrig ◽  
Rainer Kurre ◽  
Christian Schmitz ◽  
Jennifer E. Curtis ◽  
Tamás Haraszti ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Microfluidic Device ◽  
Sensor Array ◽  
Force Sensor ◽  
Optical Force ◽  
Holographic Optical Tweezers

MR Signal Intensity Calculations Are Not Reliable for Differentiating Renal Cell Carcinoma from Lipid Poor Angiomyolipoma

Radiology ◽  
2010 ◽  
Vol 257 (1) ◽  
pp. 299-300 ◽  
Author(s):  
R. Christopher Dillon ◽  
Arnold C. Friedman ◽  
Frank H. Miller
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Signal Intensity ◽  
Intensity Calculations

scholarly journals Micromanipulation of Chromosomes in PTK2 Cells Using Laser Microsurgery (Optical Scalpel) in Combination with Laser-Induced Optical Force (Optical Tweezers)

1993 ◽  
Vol 204 (1) ◽  
pp. 110-120 ◽  
Author(s):  
Hong Liang ◽  
William H. Wright ◽  
Steve Cheng ◽  
Wei He ◽  
Michael W. Berns
Keyword(s):  
Optical Tweezers ◽  
Optical Force ◽  
Laser Microsurgery
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