Red and near-infrared fluorophores inspired by chlorophylls: consideration of practical brightness in multicolor flow cytometry and biomedical sciences

2018 ◽  
Author(s):  
Rui Liu ◽  
Gongfang Hu ◽  
Masahiko Taniguchi ◽  
Hai Du ◽  
Jonathan S. Lindsey
Keyword(s):  
Flow Cytometry ◽  
Near Infrared ◽  
Biomedical Sciences ◽  
Multicolor Flow Cytometry

scholarly journals Assessment of chilling injury in hypothermic stored boar spermatozoa by multicolor flow cytometry

2021 ◽  
Author(s):  
Helen Jäkel ◽  
Heiko Henning ◽  
Anne‐Marie Luther ◽  
Karl Rohn ◽  
Dagmar Waberski
Keyword(s):  
Flow Cytometry ◽  
Chilling Injury ◽  
Multicolor Flow Cytometry ◽  
Boar Spermatozoa

scholarly journals Comparison of Lethal and Nonlethal Mouse Models of Orientia tsutsugamushi Infection Reveals T-Cell Population-Associated Cytokine Signatures Correlated with Lethality and Protection

2021 ◽  
Vol 6 (3) ◽  
pp. 121
Author(s):  
Alison Luce-Fedrow ◽  
Suchismita Chattopadhyay ◽  
Teik-Chye Chan ◽  
Gregory Pearson ◽  
John B. Patton ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Host Response ◽  
Cd8 T Cell ◽  
Cd4 T Cell ◽  
Cell Populations ◽  
Murine Models ◽  
Orientia Tsutsugamushi ◽  
Multicolor Flow Cytometry ◽  
Interstrain Difference

The antigenic diversity of Orientia tsutsugamushi as well as the interstrain difference(s) associated with virulence in mice impose the necessity to dissect the host immune response. In this study we compared the host response in lethal and non-lethal murine models of O. tsutsugamushi infection using the two strains, Karp (New Guinea) and Woods (Australia). The models included the lethal model: Karp intraperitoneal (IP) challenge; and the nonlethal models: Karp intradermal (ID), Woods IP, and Woods ID challenges. We monitored bacterial trafficking to the liver, lung, spleen, kidney, heart, and blood, and seroconversion during the 21-day challenge. Bacterial trafficking to all organs was observed in both the lethal and nonlethal models of infection, with significant increases in average bacterial loads observed in the livers and hearts of the lethal model. Multicolor flow cytometry was utilized to analyze the CD4+ and CD8+ T cell populations and their intracellular production of the cytokines IFNγ, TNF, and IL2 (single, double, and triple combinations) associated with both the lethal and nonlethal murine models of infection. The lethal model was defined by a cytokine signature of double- (IFNγ-IL2) and triple-producing (IL2-TNF-IFNγ) CD4+ T-cell populations; no multifunctional signature was identified in the CD8+ T-cell populations associated with the lethal model. In the nonlethal model, the cytokine signature was predominated by CD4+ and CD8+ T-cell populations associated with single (IL2) and/or double (IL2-TNF) populations of producers. The cytokine signatures associated with our lethal model will become depletion targets in future experiments; those signatures associated with our nonlethal model are hypothesized to be related to the protective nature of the nonlethal challenges.

scholarly journals Enhanced red and near infrared detection in flow cytometry using avalanche photodiodes

2008 ◽  
Vol 73A (8) ◽  
pp. 767-776 ◽  
Author(s):  
William G. Lawrence ◽  
Gyula Varadi ◽  
Gerald Entine ◽  
Edward Podniesinski ◽  
Paul K. Wallace
Keyword(s):  
Flow Cytometry ◽  
Near Infrared ◽  
Avalanche Photodiodes ◽  
Infrared Detection

A 16-channel avalanche photodiode detector array for visible and near-infrared flow cytometry

2006 ◽  
Author(s):  
William G. Lawrence ◽  
Christopher Stapels ◽  
Richard Farrell ◽  
Joseph D. Tario, Jr. ◽  
Edward Podniesinski ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Near Infrared ◽  
Avalanche Photodiode ◽  
Detector Array

scholarly journals Multicolor Flow Cytometry and Cytokine Analysis Provides Enhanced Information on Kidney Transplant Biopsies

2018 ◽  
Vol 3 (4) ◽  
pp. 956-969 ◽  
Author(s):  
Kimberly A. Muczynski ◽  
Nicolae Leca ◽  
Arthur E. Anderson ◽  
Niamh Kieran ◽  
Susan K. Anderson
Keyword(s):  
Flow Cytometry ◽  
Kidney Transplant ◽  
Multicolor Flow Cytometry ◽  
Cytokine Analysis

scholarly journals A novel co-culture assay to assess anti-tumor CD8+ T cell cytotoxicity via luminescence and multicolor flow cytometry

2020 ◽  
Vol 487 ◽  
pp. 112899
Author(s):  
Verónica Olivo Pimentel ◽  
Ala Yaromina ◽  
Damiënne Marcus ◽  
Ludwig J. Dubois ◽  
Philippe Lambin
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Cd8 T Cell ◽  
Cell Cytotoxicity ◽  
Multicolor Flow Cytometry ◽  
T Cell Cytotoxicity

scholarly journals Imaging Cardiovascular and Lung Macrophages With the Positron Emission Tomography Sensor 64 Cu-Macrin in Mice, Rabbits, and Pigs

2020 ◽  
Vol 13 (10) ◽  
Author(s):  
Matthias Nahrendorf ◽  
Friedrich Felix Hoyer ◽  
Anu E. Meerwaldt ◽  
Mandy M.T. van Leent ◽  
Max L. Senders ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Flow Cytometry ◽  
Confocal Microscopy ◽  
Pet Imaging ◽  
Near Infrared ◽  
Emission Tomography ◽  
Imaging Biomarker ◽  
Positron Emission ◽  
Pulmonary Macrophages

Background: Macrophages, innate immune cells that reside in all organs, defend the host against infection and injury. In the heart and vasculature, inflammatory macrophages also enhance tissue damage and propel cardiovascular diseases. Methods: We here use in vivo positron emission tomography (PET) imaging, flow cytometry, and confocal microscopy to evaluate quantitative noninvasive assessment of cardiac, arterial, and pulmonary macrophages using the nanotracer 64 Cu-Macrin—a 20-nm spherical dextran nanoparticle assembled from nontoxic polyglucose. Results: PET imaging using 64 Cu-Macrin faithfully reported accumulation of macrophages in the heart and lung of mice with myocardial infarction, sepsis, or pneumonia. Flow cytometry and confocal microscopy detected the near-infrared fluorescent version of the nanoparticle ( VT680 Macrin) primarily in tissue macrophages. In 5-day-old mice, 64 Cu-Macrin PET imaging quantified physiologically more numerous cardiac macrophages. Upon intravenous administration of 64 Cu-Macrin in rabbits and pigs, we detected heightened macrophage numbers in the infarcted myocardium, inflamed lung regions, and atherosclerotic plaques using a clinical PET/magnetic resonance imaging scanner. Toxicity studies in rats and human dosimetry estimates suggest that 64 Cu-Macrin is safe for use in humans. Conclusions: Taken together, these results indicate 64 Cu-Macrin could serve as a facile PET nanotracer to survey spatiotemporal macrophage dynamics during various physiological and pathological conditions. 64 Cu-Macrin PET imaging could stage inflammatory cardiovascular disease activity, assist disease management, and serve as an imaging biomarker for emerging macrophage-targeted therapeutics.

Determination of Circulating Endothelial Cells and Endothelial Progenitor Cells Using Multicolor Flow Cytometry in Patients with Thrombophilia

2019 ◽  
Vol 142 (2) ◽  
pp. 113-119
Author(s):  
Martin Řádek ◽  
Eva Babuňková ◽  
Martin Špaček ◽  
Tomáš Kvasnička ◽  
Jan Kvasnička
Keyword(s):  
Flow Cytometry ◽  
Endothelial Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Damage ◽  
Circulating Endothelial Cells ◽  
High Dose ◽  
Multicolor Flow Cytometry ◽  
Endothelial Progenitor ◽  
Identification And Quantification

Background/Aims: Endothelial progenitor cells (EPCs) and circulating endothelial cells (CECs) have been described as markers of endothelial damage and dysfunction in several diseases, including deep venous thrombosis. Their role in patients with known thrombophilia has not yet been evaluated. Both EPCs and CECs represent extremely rare cell populations. Therefore, it is essential to use standardized methods for their identification and quantification. Methods: In this study, we used multicolor flow cytometry to analyze the number of EPCs and CECs in patients with thrombophilia with or without a history of thrombosis. Patients with hematological malignancies after high-dose chemotherapy and patients with acute myocardial infarction were used as positive controls. Results: EPC and CEC immunophenotypes were determined as CD45dim/–CD34+CD146+CD133+ and CD45dim/–CD34+CD146+CD133–, respectively. Increased levels of endothelial cells were observed in positive control groups. No significant changes in the number of EPCs or CECs were detected in patients with thrombophilia compared to healthy controls. Conclusion: Our optimized multicolor flow cytometry method allows unambiguous identification and quantification of endothelial cells in the peripheral blood. Our results support previous studies showing that elevated levels of CECs could serve as an indicator of endothelial injury or dysfunction. Normal levels of CECs or EPCs were found in patients with thrombophilia.

Sign in / Sign up

Export Citation Format

Share Document