Optical properties of chlorin e6 in living melanoma cells

Chlorin-[Formula: see text] dimethyl esters possessing a single carboxy group at the 13-, 151-, or 172-position were prepared by chemically modifying chlorophyll-[Formula: see text]. These three synthetic regioisomers were fully characterized by their mass, NMR, and visible absorption spectra. Their molecular structures were unambiguously identified by the specific 1H–[Formula: see text]C correlation at the 13-, 15-, and/or 17-substituents in their respective HMBC spectra. Methyl esterification of 13/151-COOH and hydrolysis of 13/151-COOMe affected small shifts of the Qy absorption and fluorescence emission maxima in a diluted CH2Cl2 solution, while no substitution effect of 172-COOH/Me was observed.

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SONODYNAMIC EFFECT IN A375 MELANOMA CELLS WITH CHLORIN E6 INDUCED BY 20 kHz ULTRASOUND

Journal of Physics D Applied Physics ◽

10.1088/1361-6463/ac2f16 ◽

2021 ◽

Author(s):

Antonina Dadadzhanova ◽

Ekaterina Kolesova ◽

Vladimir Maslov ◽

Eliz Amar-Lewis ◽

Riki Goldbart ◽

...

Keyword(s):

Melanoma Cells ◽

Chlorin E6 ◽

A375 Melanoma

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Photophysics of chlorin e6: from one- and two-photon absorption to fluorescence and phosphorescence

RSC Advances ◽

10.1039/c6ra28616j ◽

2017 ◽

Vol 7 (18) ◽

pp. 10992-10999 ◽

Cited By ~ 18

Author(s):

Hugo Gattuso ◽

Antonio Monari ◽

Marco Marazzi

Keyword(s):

Optical Properties ◽

Singlet Oxygen ◽

Photon Absorption ◽

Chlorin E6 ◽

Two Photon Absorption ◽

Two Photon ◽

Non Linear ◽

Linear Optical Properties ◽

Linear Optical ◽

Fluorescence And Phosphorescence

Linear and non-linear optical properties of a known photosensitizer producing singlet oxygen, chlorin e6, have been studied, including dynamics effects.

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Optical properties of conjugates of CdSe/ZnS quantum dots and chlorin e6 in aqueous solution

Journal of Optical Technology ◽

10.1364/jot.82.000738 ◽

2015 ◽

Vol 82 (11) ◽

pp. 738 ◽

Cited By ~ 1

Author(s):

A. K. Visheratina ◽

A. O. Orlova ◽

V. G. Maslov ◽

A. V. Fedorov ◽

A. V. Baranov

Keyword(s):

Aqueous Solution ◽

Quantum Dots ◽

Optical Properties ◽

Chlorin E6

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Design of objective lens for 200-kV high-resolution electron microscopes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075361 ◽

1983 ◽

Vol 41 ◽

pp. 314-315

Author(s):

K. Tsuno ◽

T. Honda ◽

Y. Harada ◽

M. Naruse

Keyword(s):

Optical Properties ◽

Computer Technology ◽

Axial Magnetic Field ◽

Chromatic Aberration ◽

Objective Lens ◽

Resolution Electron ◽

Correction Of Astigmatism ◽

Three Stages ◽

Electron Microscopes ◽

Stage 1

Developement of computer technology provides much improvements on electron microscopy, such as simulation of images, reconstruction of images and automatic controll of microscopes (auto-focussing and auto-correction of astigmatism) and design of electron microscope lenses by using a finite element method (FEM). In this investigation, procedures for simulating the optical properties of objective lenses of HREM and the characteristics of the new lens for HREM at 200 kV are described.The process for designing the objective lens is divided into three stages. Stage 1 is the process for estimating the optical properties of the lens. Firstly, calculation by FEM is made for simulating the axial magnetic field distributions Bzc of the lens. Secondly, electron ray trajectory is numerically calculated by using Bzc. And lastly, using Bzc and ray trajectory, spherical and chromatic aberration coefficients Cs and Cc are numerically calculated. Above calculations are repeated by changing the shape of lens until! to find an optimum aberration coefficients.

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Optical Properties of HVEM Accelerators

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100114529 ◽

1975 ◽

Vol 33 ◽

pp. 180-181

Author(s):

A. Strojnik ◽

J.W. Scholl ◽

V. Bevc

Keyword(s):

Optical Properties ◽

Electron Accelerator ◽

Systematic Investigation ◽

Electron Source ◽

High Brightness ◽

The Past ◽

Wide Range ◽

Optical Behavior

The electron accelerator, as inserted between the electron source (injector) and the imaging column of the HVEM, is usually a strong lens and should be optimized in order to ensure high brightness over a wide range of accelerating voltages and illuminating conditions. This is especially true in the case of the STEM where the brightness directly determines the highest resolution attainable. In the past, the optical behavior of accelerators was usually determined for a particular configuration. During the development of the accelerator for the Arizona 1 MEV STEM, systematic investigation was made of the major optical properties for a variety of electrode configurations, number of stages N, accelerating voltages, 1 and 10 MEV, and a range of injection voltages ϕ0 = 1, 3, 10, 30, 100, 300 kV).

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Differential polarization imaging of sickled cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102390 ◽

1988 ◽

Vol 46 ◽

pp. 62-63

Author(s):

Marcos F. Maestre

Keyword(s):

Optical Properties ◽

Theoretical Approach ◽

Polarized Light ◽

Polarization Microscopy ◽

Spectroscopic Techniques ◽

Polarization Imaging ◽

Circularly Polarized Light ◽

Circularly Polarized ◽

Microscopic Scale ◽

Chiral Structures

Recently we have developed a form of polarization microscopy that forms images using optical properties that have previously been limited to macroscopic samples. This has given us a new window into the distribution of structure on a microscopic scale. We have coined the name differential polarization microscopy to identify the images obtained that are due to certain polarization dependent effects. Differential polarization microscopy has its origins in various spectroscopic techniques that have been used to study longer range structures in solution as well as solids. The differential scattering of circularly polarized light has been shown to be dependent on the long range chiral order, both theoretically and experimentally. The same theoretical approach was used to show that images due to differential scattering of circularly polarized light will give images dependent on chiral structures. With large helices (greater than the wavelength of light) the pitch and radius of the helix could be measured directly from these images.

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Sequential detection of mRNA for the chemokine IL-8 and macrophages (F4/80) in human melanoma

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010014141x ◽

1995 ◽

Vol 53 ◽

pp. 1008-1009

Author(s):

C.D. Bucana ◽

R. Sanchez ◽

R. Singh ◽

I.J. Fidler

Keyword(s):

Tumor Cells ◽

Nude Mice ◽

Constitutive Expression ◽

Metastatic Potential ◽

Melanoma Cells ◽

Human Melanoma ◽

Angiogenic Factor ◽

Infiltrating Cells ◽

Immunoperoxidase Assay ◽

Multifunctional Cytokine

The purpose of this study was to demonstrate by ISH the presence of IL-8 mRNA, and by immunohistochemistry (IHC) the presence of the chemokine IL-8 and the distribution of infiltrating macrophages in subcutaneous melanomas in the same tumor. IL-8 is a multifunctional cytokine produced by melanoma cells, activated macrophages and monocytes and it has been shown to be a growth and angiogenic factor for tumor cells. More recently it was shown that constitutive expression of IL-8 correlated directly with metastatic potential of human melanoma cells in nude mice. IL-8 content of a solid tumor as determined by Western blot analysis does not take into account the contribution of macrophages. Previous studies showed that murine tumors contain many infiltrating cells interspersed among tumor cells whereas human tumors growing in nude mice exhibit macrophages at the periphery or between tumor islands. In this study we demonstrate the expression of IL-8 and the distribution of macrophages by immunoperoxidase assay and IL-8 mRNA by ISH.

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A transmission electron microscopic study of structural transitions in fast ionic conductors

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100125713 ◽

1987 ◽

Vol 45 ◽

pp. 156-157

Author(s):

R. B. Queenan ◽

P. K. Davies

Keyword(s):

Optical Properties ◽

Ionic Conduction ◽

Recent Observation ◽

Ionic Conductivities ◽

Sodium Aluminate ◽

Two Dimensions ◽

Ionic Conductors ◽

Electron Microscopic ◽

Transmission Electron ◽

Trivalent Cations

Na ß“-alumina (Na1.67Mg67Al10.33O17) is a non-stoichiometric sodium aluminate which exhibits fast ionic conduction of the Na+ ions in two dimensions. The Na+ ions can be exchanged with a variety of mono-, di-, and trivalent cations. The resulting exchanged materials also show high ionic conductivities.Considerable interest in the Na+-Nd3+-ß“-aluminas has been generated as a result of the recent observation of lasing in the pulsed and cw modes. A recent TEM investigation on a 100% exchanged Nd ß“-alumina sample found evidence for the intergrowth of two different structure types. Microdiffraction revealed an ordered phase coexisting with an apparently disordered phase, in which the cations are completely randomized in two dimensions. If an order-disorder transition is present then the cooling rates would be expected to affect the microstructures of these materials which may in turn affect the optical properties. The purpose of this work was to investigate the affect of thermal treatments upon the micro-structural and optical properties of these materials.

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