Super-resolution ultrasound imaging beyond the acoustic diffraction limit

2019 ◽  
Vol 145 (3) ◽  
pp. 1703-1703
Author(s):  
Kang Kim ◽  
Qiyang Chen ◽  
Jaesok Yu
Keyword(s):  
Ultrasound Imaging ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Acoustic Diffraction

scholarly journals Current Development and Applications of Super-Resolution Ultrasound Imaging

Sensors ◽  
2021 ◽  
Vol 21 (7) ◽  
pp. 2417
Author(s):  
Qiyang Chen ◽  
Hyeju Song ◽  
Jaesok Yu ◽  
Kang Kim
Keyword(s):  
Kidney Disease ◽  
Ultrasound Imaging ◽  
Atherosclerotic Plaque ◽  
Super Resolution ◽  
Emerging Technology ◽  
Diffraction Limit ◽  
Disease Models ◽  
Current Development ◽  
Future Directions ◽  
Acoustic Diffraction

Abnormal changes of the microvasculature are reported to be key evidence of the development of several critical diseases, including cancer, progressive kidney disease, and atherosclerotic plaque. Super-resolution ultrasound imaging is an emerging technology that can identify the microvasculature noninvasively, with unprecedented spatial resolution beyond the acoustic diffraction limit. Therefore, it is a promising approach for diagnosing and monitoring the development of diseases. In this review, we introduce current super-resolution ultrasound imaging approaches and their preclinical applications on different animals and disease models. Future directions and challenges to overcome for clinical translations are also discussed.

scholarly journals Acousto optic imaging beyond the acoustic diffraction limit using speckle decorrelation

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Daniel Doktofsky ◽  
Moriya Rosenfeld ◽  
Ori Katz
Keyword(s):  
Scattered Light ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Resolution Limit ◽  
Contrast Imaging ◽  
Optical Contrast ◽  
Ultrasound Modulation ◽  
Wavefront Shaping ◽  
Acoustic Diffraction ◽  
Natural Dynamics

AbstractAcousto-optic imaging (AOI) enables optical-contrast imaging deep inside scattering samples via localized ultrasound modulation of scattered light. However, the resolution in AOI is inherently limited by the ultrasound focus size, prohibiting microscopic investigations. In recent years advances in the field of digital wavefront-shaping allowed the development of novel approaches for overcoming AOI’s acoustic resolution limit. However, these approaches require thousands of wavefront measurements within the sample speckle decorrelation time, limiting their application to static samples. Here, we show that it is possible to surpass the acoustic resolution-limit with a conventional AOI system by exploiting the natural dynamics of speckle decorrelations rather than trying to overcome them. We achieve this by adapting the principles of super-resolution optical fluctuations imaging (SOFI) to AOI. We show that naturally fluctuating optical speckle grains can serve in AOI as the analogues of blinking fluorophores in SOFI, enabling super-resolution by statistical analysis of fluctuating acousto-optic signals.

scholarly journals Super-resolution Ultrasound Imaging of the Renal Microvasculature in Rats with Metabolic syndrome

2020 ◽  
Author(s):  
Stinne Byrholdt Sogaard ◽  
Sofie Bech Andersen ◽  
Iman Taghavi ◽  
Carlos Armando Villagomez Hoyos ◽  
Kristoffer Lindskov Hansen ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Ultrasound Imaging ◽  
Super Resolution ◽  
Renal Microvasculature

scholarly journals MINSTED fluorescence localization and nanoscopy

2021 ◽  
Author(s):  
Michael Weber ◽  
Marcel Leutenegger ◽  
Stefan Stoldt ◽  
Stefan Jakobs ◽  
Tiberiu S. Mihaila ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Stimulated Emission ◽  
Far Field ◽  
Inner Membrane ◽  
Mitochondrial Inner Membrane ◽  
Molecular Scale ◽  
Localization Precision ◽  
Super Resolution Microscopy

AbstractWe introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point of minimal STED, serves as a movable reference coordinate for fluorophore localization. As the STED rate, the background and the required number of fluorescence detections are low compared with most other STED microscopy and localization methods, MINSTED entails substantially less fluorophore bleaching. In our implementation, 200–1,000 detections per fluorophore provide a localization precision of 1–3 nm in standard deviation, which in conjunction with independent single fluorophore switching translates to a ~100-fold improvement in far-field microscopy resolution over the diffraction limit. The performance of MINSTED nanoscopy is demonstrated by imaging the distribution of Mic60 proteins in the mitochondrial inner membrane of human cells.

Adaptive Multifocus Beamforming for Contrast-Enhanced-Super-Resolution Ultrasound Imaging in Deep Tissue

2018 ◽  
Vol 65 (12) ◽  
pp. 2255-2263 ◽  
Author(s):  
David Espindola ◽  
Fanglue Lin ◽  
Danai E. Soulioti ◽  
Paul A. Dayton ◽  
Gianmarco F. Pinton
Keyword(s):  
Ultrasound Imaging ◽  
Super Resolution ◽  
Deep Tissue ◽  
Contrast Enhanced

An overview of structured illumination microscopy: recent advances and perspectives

2021 ◽  
Author(s):  
Krishnendu Samanta ◽  
Joby Joseph
Keyword(s):  
Spatial Frequency ◽  
Basic Principle ◽  
Imaging Techniques ◽  
Resolution Enhancement ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Structured Illumination ◽  
Post Processing ◽  
Structured Illumination Microscopy ◽  
Near Future

Abstract Structured illumination microscopy (SIM) is one of the most significant widefield super-resolution optical imaging techniques. The conventional SIM utilizes a sinusoidal structured pattern to excite the fluorescent sample; which eventually down-modulates higher spatial frequency sample information within the diffraction-limited passband of the microscopy system and provides around two-fold resolution enhancement over diffraction limit after suitable computational post-processing. Here we provide an overview of the basic principle, image reconstruction, technical development of the SIM technique. Nonetheless, in order to push the SIM resolution further towards the extreme nanoscale dimensions, several different approaches are launched apart from the conventional SIM. Among the various SIM methods, some of the important techniques e.g. TIRF, non-linear, plasmonic, speckle SIM etc. are discussed elaborately. Moreover, we highlight different implementations of SIM in various other imaging modalities to enhance their imaging performances with augmented capabilities. Finally, some future outlooks are mentioned which might develop fruitfully and pave the way for new discoveries in near future.

scholarly journals Super-resolution microscopy: successful applications in centrosome study and beyond

2019 ◽  
Vol 5 (5-6) ◽  
pp. 235-243 ◽  
Author(s):  
Jingyan Fu ◽  
Chuanmao Zhang
Keyword(s):  
Molecular Basis ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Animal Cells ◽  
Microtubule Organizing Center ◽  
The Past ◽  
Eukaryotic Species ◽  
Organizing Center ◽  
Cilia And Flagella ◽  
Super Resolution Microscopy

AbstractCentrosome is the main microtubule-organizing center in most animal cells. Its core structure, centriole, also assembles cilia and flagella that have important sensing and motility functions. Centrosome has long been recognized as a highly conserved organelle in eukaryotic species. Through electron microscopy, its ultrastructure was revealed to contain a beautiful nine-symmetrical core 60 years ago, yet its molecular basis has only been unraveled in the past two decades. The emergence of super-resolution microscopy allows us to explore the insides of a centrosome, which is smaller than the diffraction limit of light. Super-resolution microscopy also enables the compartmentation of centrosome proteins into different zones and the identification of their molecular interactions and functions. This paper compiles the centrosome architecture knowledge that has been revealed in recent years and highlights the power of several super-resolution techniques.

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