Plasma B-6 Vitamer Changes Following a 50-km Ultramarathon

2000 ◽  
Vol 10 (3) ◽  
pp. 302-314 ◽  
Author(s):  
Scott W. Leonard ◽  
James E. Leklem
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Plasma Glucose ◽  
Plasma Concentrations ◽  
Blood Samples ◽  
Liquid Intake ◽  
Physical Conditions ◽  
The Mean

The purpose of this study was to measure plasma B-6 vitamers, and other factors which may affect the plasma concentrations of these vitamers under extreme physical conditions. Blood samples were drawn from 8 men and 3 women (43.7 ± 8.6 years) 30 min prior to the start of a 50-km ultramarathon race (pre), and at 5 (PST) and 60 (PST60) min post race. HPLC was used to measure plasma pyridoxal 5’-phosphate (PLP), pyridoxal (PL), pyridoxine (PN), and 4-pyridoxic acid (4-PA). Plasma glucose, albumin, lactate, and alkaline phosphatase activity, as well as hematocrit, and hemoglobin levels were measured. Food and liquid intake was assessed during the run. There was a significant (p < .001) decrease in the plasma PLP concentration between pre and PST, with a mean decrease of 12.9 ± 8.8 nmol/L (31% decrease). At PST60, there was a further decrease in plasma PLP concentration bringing the total decrease to 17.9 nmol/L (44%). The plasma TB6 concentration also decreased after the run, but the mean decrease was only 13.5 nmol/L (pre to PST60). PL increased 25% after the run, and did not change further at PST60. The mean plasma 4-PA concentration increased 21% post run and decreased to just below the pre-run value 1 hr post race. The plasma PLP decrease measured in the current study is not consistent with what has previously been reported during shorter length endurance studies.

Decreased Abundance of Alkaline Phosphatase and Calbindin-D9K mRNAs in the Intestine of the Spontaneously Hypertensive Rat

1992 ◽  
Vol 38 (12) ◽  
pp. 2526-2531 ◽  
Author(s):  
D T Leetun ◽  
M E Bruns ◽  
D E Bruns
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Specific Activity ◽  
Spontaneously Hypertensive Rat ◽  
Similar Extent ◽  
Spontaneously Hypertensive ◽  
Wky Rats ◽  
Calbindin D9k ◽  
The Mean

Abstract Alkaline phosphatase activity and calbindin-D9K immunoreactivity are decreased in the intestines of spontaneously hypertensive rats (SHRs). To investigate the potential role of altered gene expression in these decreases, we measured, by Northern blot analyses, the abundances of alkaline phosphatase and calbindin-D9K mRNAs in the proximal regions of the small intestines of 14-week-old SHR and control Wistar-Kyoto (WKY) rats. Alternate 4-cm segments of intestine were used for measurements of the proteins (0-4 cm, 8-12 cm, and 16-20 cm from pylorus, segments A1, B1, and C1, respectively) and mRNAs (4-8 cm, 12-16 cm, and 20-24 cm, segments A2, B2, and C2). Calbindin-D9K (immunoassay) was decreased in SHR vs WKY rats by 27%, 64%, and 67% in segments A1, B1, and C1, respectively (P &lt; 0.01); its mRNA was decreased to a similar extent (69%, 82%, and 80%, respectively; P &lt; 0.002 by analysis of variance). Alkaline phosphatase activity was decreased in SHRs by 58%, 54%, and 51% in segments A1, B1, and C1, respectively (P &lt; 0.01); the abundance of its 3.0-kb mRNA was decreased to a similar extent: 57%, 80%, and 69% in segments A2, B2, and C2, respectively (P &lt; 0.02). The mean decreases of the 2.7-kb mRNA of alkaline phosphatase were statistically significant (P &lt; 0.02) but smaller (38%, 40%, and 35%). The mean abundance of vitamin D receptor mRNA in the same animals was decreased slightly in SHR vs WKY rats (3%, 36%, and 20% in segments A2, B2, and C2, respectively), but the difference in the values was not statistically significant. Decreases in alkaline phosphatase activity and calbindin-D9K immunoreactivity may reflect decreased mRNA abundance and not decreased enzyme-specific activity or increased protein degradation.

scholarly journals Reproductive Toxicity to Male Mice of Nose Only Exposure to Water- Pipe Smoke

2015 ◽  
Vol 35 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Badreldin H. Ali ◽  
Sirin A. Adham ◽  
Khalid A. Al Balushi ◽  
Asem Shalaby ◽  
Mostafa I. Waly ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Plasma Concentrations ◽  
Reproductive Toxicity ◽  
Growth Factor Receptor ◽  
The Body ◽  
Water Pipe ◽  
Endothelial Growth Factor

Background/Aims: Water-pipe smoking (WPS) is popular in the Middle East and is starting to gain popularity in several Western countries as well. It is widely and erroneously perceived to be less harmful than other forms of tobacco use. The reproductive adverse effects of cigarette smoking have been studied before with conflicting results, but data on the possible adverse reproductive effects of WPS are lacking. Here, we assessed the effects of nose-only exposure to mainstream WPS generated by commercially available honey-flavored "moasel" tobacco in mice. Methods: The duration of the session was 30 min/day for one month. Control mice were exposed to air. Twenty- four h after the last exposure, mice were killed and the testes and plasma removed for analysis. In testicular homogenates total protein, alkaline phosphatase activity, several indices of oxidative damage and Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) were quantified. The plasma concentrations of leptin, testosterone, estrogen and luteinizing hormone (LH) were also measured. Histological analysis of testes and lungs was also conducted. Results: WPS caused statistically significant decreases in the plasma concentrations of leptin, testosterone, and LH, and in the concentrations of total protein and the antioxidant indices measured. A statistically non - significant decrease in VEGFR2 protein in the WPS - exposed mice compared to the control mice was also found. The body and testicular weights of mice exposed to WPS, as well as their testicular alkaline phosphatase activity and light microscopic histology, and plasma estrogen concentration were all not significantly affected by WPS. Conclusion: Further studies on the functional implications of these findings in mice exposed to WPS for longer durations are warranted.

scholarly journals Alkaline Phosphatase and Disaccharide Activity in Barrett's Columnar Lined Esophagus

1988 ◽  
Vol 2 (1) ◽  
pp. 15-17
Author(s):  
S.N. Sullivan ◽  
R.J. Darwish ◽  
M. Troster
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Biochemical Markers ◽  
Squamous Epithelium ◽  
Disaccharidase Activity ◽  
Columnar Lined Esophagus ◽  
The Mean ◽  
Small Intestinal ◽  
Standard Deviations

Esophageal mucosal biopsies of 15 patients with normal squamous epithelium, 11 with inflamed squamous epithelium and 23 with Barrete's columnar lined esophagus were assayed for alkaline phosphatase and disaccharidase activity. Seven of the patients with Barrett's esophagus had alkaline phosphatase activity greater than three standard deviations above the mean of the 15 patients with normal squamous epithelium. Five of these seven also had disaccharidase activity three standard deviations or greater above normal. One patient with esophagitis, but without columnar epithelium, had a small increase in alkaline phosphatase and disaccharidase activity. The production of biochemical markers of small intestinal structure may he another indicator of the pluripotential nature of Barrett's epithelium.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

Sign in / Sign up

Export Citation Format

Share Document