scholarly journals Vaccine-mediated protection against Campylobacter-associated enteric disease

2020 ◽  
Vol 6 (26) ◽  
pp. eaba4511
Author(s):  
Benjamin K. Quintel ◽  
Kamm Prongay ◽  
Anne D. Lewis ◽  
Hans-Peter Raué ◽  
Sara Hendrickson ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Capsular Polysaccharide ◽  
Vaccine Development ◽  
Rhesus Macaques ◽  
Campylobacter Coli ◽  
Enteric Disease ◽  
Important Advance ◽  
New Approach ◽  
Naturally Occurring ◽  
Bacterial Gastroenteritis

Campylobacter coli and Campylobacter jejuni are responsible for 400 million to 500 million cases of enteric disease each year and represent the most common cause of bacterial gastroenteritis worldwide. Despite its global importance, Campylobacter vaccine development has been hampered by the lack of animal models that recapitulate human disease pathogenesis. Here, we describe a naturally occurring Campylobacter-associated diarrhea model in outdoor-housed rhesus macaques. Using this model, we developed novel next-generation H2O2-based Campylobacter vaccines that induced strong antibacterial antibodies to multiple Campylobacter proteins including flagellin and provided up to 83% protection against severe C. coli–associated diarrhea. Whole-genome sequencing of circulating Campylobacter strains revealed little to no homology within lipooligosaccharide or capsular polysaccharide loci with the Campylobacter vaccine strains used in these studies, indicating that vaccine-mediated immunity was not restricted to a single homologous serotype. Together, these results demonstrate an important advance in vaccine development and a new approach to reducing Campylobacter-associated enteric disease.

scholarly journals Enumeration and Diversity of Campylobacters and Bacteriophages Isolated during the Rearing Cycles of Free-Range and Organic Chickens

2005 ◽  
Vol 71 (3) ◽  
pp. 1259-1266 ◽  
Author(s):  
A. El-Shibiny ◽  
P. L. Connerton ◽  
I. F. Connerton
Keyword(s):  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Pulsed Field Gel Electrophoresis ◽  
Campylobacter Coli ◽  
Potential Influence ◽  
Group Iii ◽  
Free Range ◽  
Naturally Occurring

ABSTRACT Campylobacters and Campylobacter-specific bacteriophages were isolated and enumerated during the rearing cycle of free-range (56 days) and organic chickens (73 days) at 3-day intervals from hatching until slaughter. In both flocks Campylobacter jejuni was the initial colonizer but Campylobacter coli was detected more frequently from 5 weeks of age. The diversity of the Campylobacter isolates was examined by pulsed-field gel electrophoresis of SmaI-digested genomic DNA and antimicrobial resistance typing. Bacteriophages were isolated from 51% (19 of 37 birds) of Campylobacter-positive organic birds (log10 2.5 to log10 5.7 PFU/g of cecal contents). The bacteriophages were all typical group III Campylobacter bacteriophages in terms of genomic size but could be characterized in terms of their host range and placed into five different groups. In contrast to the organic birds, anti-Campylobacter activity (bacteriocin-like) was observed in 26% (10 of 38 birds) of Campylobacter-positive free-range birds, and only one bacteriophage was isolated. Appearance of either bacteriophages or anti-Campylobacter activity was associated with changes in the levels of colonization and the predominant genotypes and species isolated. The frequency and potential influence of naturally occurring bacteriophages and/or inhibitory substances on the diversity and fluctuations of populations of campylobacters have not previously been reported in either free-range or organic chickens.

Reduction of Campylobacter jejuni and Campylobacter coli in Poultry Skin by Fruit Extracts

2010 ◽  
Vol 73 (3) ◽  
pp. 477-482 ◽  
Author(s):  
DIANA VALTIERRA-RODRÍGUEZ ◽  
NORMA L. HEREDIA ◽  
SANTOS GARCÍA ◽  
EDUARDO SÁNCHEZ
Keyword(s):  
Antimicrobial Activity ◽  
Campylobacter Jejuni ◽  
Antimicrobial Agents ◽  
Orange Peel ◽  
Campylobacter Coli ◽  
Sour Orange ◽  
Bacterial Gastroenteritis ◽  
Natural Foods ◽  
Promising Source

Campylobacter spp. are a major cause of foodborne bacterial gastroenteritis in humans, and current methods to control Campylobacter contamination in foods are not completely successful. Plants are a promising source of antimicrobial agents, particularly given the growing interest in “all natural” foods. In this study, the antimicrobial activity of extracts from 28 edible plants against Campylobacter jejuni and Campylobacter coli was evaluated in vitro and in a poultry skin model. Nine of 28 extracts exhibited antimicrobial activity in a diffusion assay, and MBCs were determined for the three most active extracts, i.e., lime, plum, and sour orange peel (MBCs of 2 to 3 mg/ml). Mixtures of the lime, plum, and sour orange peel extracts were applied to chicken skin inoculated with 105 CFU of Campylobacter to test for synergistic or antagonist effects. After incubation (48 h at 4°C) with any extract mixture, no Campylobacter CFUs were detectable. A panel of tasters determined that the mixture of lime and plum gave the best flavor to chicken wings. These active extracts from edible fruits are simple to prepare and are alternatives to reduce or eliminate Campylobacter contamination of chicken products.

scholarly journals Genetics behind the biosynthesis of nonulosonic acid containing lipooligosaccharides inCampylobacter coli

2018 ◽  
Author(s):  
Alejandra Kolehmainen ◽  
Mirko Rossi ◽  
Jacek Stupak ◽  
Jianjun Li ◽  
Michel Gilbert ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Campylobacter Coli ◽  
Strong Impact ◽  
Large Set ◽  
Donor And Acceptor ◽  
Nonulosonic Acid ◽  
Bacterial Gastroenteritis ◽  
Acute Polyneuropathy

ABSTRACTCampylobacter jejuniandCampylobacter coliare the most common cause of bacterial gastroenteritis in the world. Ganglioside mimicry byC. jejunilipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy. Sialyltransferases from the glycosyltransferase (GT) family 42 are essential for the expression of ganglioside mimics inC. jejuni. Recently, two novel GT-42 genes,cstIVandcstV, have been identified inC. coli.Despite being present in ∼11% of currently availableC. coligenomes, the biological role ofcstIVandcstVis unknown. In the present study, mutation studies in two strains expressing eithercstIVorcstVwere performed and mass spectrometry was used to investigate differences in the chemical composition of LOS. Attempts were made to identify donor and acceptor molecules usingin vitroactivity tests with recombinant GT-42 enzymes. Here, we show that CstIV and CstV are involved inC. coliLOS biosynthesis. In particular,cstVis associated with LOS sialylation, whilecstIVis linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCEDespite being a major foodborne pathogen,Campylobacter coliglycobiology has been largely neglected. The genetic makeup of theC. colilipooligosaccharide biosynthesis locus was largely unknown until recently.C. coliharbour a large set of genes associated to lipooligosaccharide biosynthesis, including several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide inCampylobacter jejuni. In the present study,C. coliwas found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids. These findings have a strong impact in understandingC. coliecology, host-pathogen interaction, and pathogenesis.

scholarly journals Origin, evolution, and distribution of the molecular machinery for biosynthesis of sialylated lipooligosaccharide structures in Campylobacter coli

2017 ◽  
Author(s):  
Alejandra Culebro ◽  
Miguel P. Machado ◽  
João André Carriço ◽  
Mirko Rossi
Keyword(s):  
Sialic Acid ◽  
Small Group ◽  
Campylobacter Jejuni ◽  
Causative Agent ◽  
Etiological Agent ◽  
Campylobacter Coli ◽  
Common Cause ◽  
Molecular Machinery ◽  
Bacterial Gastroenteritis ◽  
Triggering Factor

ABSTRACTCampylobacter jejuni and Campylobacter coli are the most common cause of bacterial gastroenteritis worldwide. Additionally, C. jejuni is the most common bacterial etiological agent in the autoimmune Guillain-Barré syndrome (GBS). Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of the disease. LOS-associated genes involved in the synthesis (neuABC) and transfer of sialic acid (sialyltranferases) are essential in C. jejuni to synthesize ganglioside-like LOS. Therefore these genes have been identified as GBS markers. So far, scarce genetic evidence supports C. coli as a GBS causative agent despite being isolated from GBS patients. Here we show that genes putatively involved in sialic acid transfer are widely distributed in the C. coli population. Evidence found herein suggests that a small group of C. coli strains are very likely to express ganglioside mimics, implying that C. coli can potentially trigger GBS. C. coli also presents a larger repertoire of sialyltransferases than C. jejuni and loss of functions of some those LOS-associated genes has happened during adaptation to agriculture niche. Nevertheless, the activity of these sialyltransferases and their role in shaping C. coli population is yet to be explored.

New approach for quantification of signal molecules AI-2 of Campylobacter jejuni

2020 ◽  
Author(s):  
Anja Klančnik ◽  
Iztok Dogša ◽  
Sonja Smole Možina ◽  
Dina Ramić
Keyword(s):  
Campylobacter Jejuni ◽  
Signal Molecules ◽  
New Approach
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