scholarly journals Delta glutamate receptors are functional glycine- and ᴅ-serine–gated cation channels in situ

2021 ◽  
Vol 7 (52) ◽  
Author(s):  
Elisa Carrillo ◽  
Cuauhtemoc U. Gonzalez ◽  
Vladimir Berka ◽  
Vasanthi Jayaraman
Keyword(s):  
Glutamate Receptors ◽  
Cation Channels

cGMP stimulates sodium and chloride currents in rat tracheal airway epithelia

1997 ◽  
Vol 272 (3) ◽  
pp. C911-C922 ◽  
Author(s):  
E. M. Schwiebert ◽  
E. D. Potter ◽  
T. H. Hwang ◽  
J. S. Woo ◽  
C. Ding ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Epithelial Cells ◽  
Ion Transport ◽  
Cation Channels ◽  
Enzyme Analysis ◽  
Airway Epithelial ◽  
Cyclic Monophosphate ◽  
Tracheal Epithelial Cells ◽  
Tracheal Epithelial

To test the hypothesis that guanosine 3',5'-cyclic monophosphate (cGMP) regulates ion transport in airway epithelial cells, we measured short-circuit current (I(sc)) and (22)Na+ fluxes in primary cultured rat tracheal epithelial cells. In Cl- -containing Ringer solution, I(sc) was increased by approximately 17 microA/cm2 after application of 1 mM 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), whereas, in Cl- -free solutions, the Na+ -mediated component was approximately 5 microA/cm2, suggesting a cGMP stimulation of Cl-secretory current and a smaller Na+ absorptive current. Inward and net mucosal-to-serosal (22)Na+ flux was doubled in the presence of 2 mM 8-BrcGMP. To determine whether nucleotide-gated channels play a role in this transepithelial Na+ absorption, blockers of nucleotide-gated cation channels were used to inhibit I(sc). The cGMP-stimulated Na+-mediated I(sc) was blocked by as little as 500 nM dichlorobenzamil or 50 microM L-cis-diltiazem, which are known blockers for cyclic nucleotide-gated cation channels. These agents also blocked the basal (non-cGMP-stimulated) current when measured in the presence of 10 microM amiloride, which blocks current through 5-pS amiloride-sensitive Na+ channels. To document whether the distribution of nucleotide-gated nonselective cation channels was consistent with a role in airway epithelial transport, in situ hybridization was performed. In situ hybridization of mRNA encoding for nucleotide-gated cation channels was found in epithelial cell layers of rat trachea, bronchi, bronchioles, and alveolar cells but not in smooth muscle layers or tracheal cartilage. Reverse transcriptase-polymerase chain reaction, restriction enzyme analysis, and sequencing of the cDNA transcribed from mRNA of whole lung and tracheal epithelial cells indicate that a channel highly homologous to the retinal nucleotide-gated nonselective cation channel (CNG1) is present. Thus these data, along with evidence supporting the existence of signal transduction pathways elevating intracellular levels of cGMP, indicate that cGMP regulates transepithelial ion transport in lung epithelial tissues.

The mRNA expression of AMPA type glutamate receptors in the primary motor cortex of patients with amyotrophic lateral sclerosis: an in situ hybridization study

2004 ◽  
Vol 360 (3) ◽  
pp. 170-174 ◽  
Author(s):  
Susanne Petri ◽  
Klaus Krampfl ◽  
Fariba Hashemi ◽  
Sonja Schmalbach ◽  
Claudia Grothe ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
In Situ Hybridization ◽  
Motor Cortex ◽  
Mrna Expression ◽  
Glutamate Receptors ◽  
Primary Motor Cortex ◽  
Hybridization Study ◽  
Lateral Sclerosis

Extracellular alkaline transients mediated by glutamate receptors in the rat hippocampal slice are not due to a proton conductance

1994 ◽  
Vol 72 (4) ◽  
pp. 2031-2033 ◽  
Author(s):  
P. Paalasmaa ◽  
T. Taira ◽  
J. Voipio ◽  
K. Kaila
Keyword(s):  
Glutamate Receptors ◽  
Ampa Receptors ◽  
Hippocampal Slice ◽  
Aminobutyric Acid ◽  
Cation Channels ◽  
Ionic Mechanism ◽  
Synaptic Activation ◽  
Gamma Aminobutyric Acid ◽  
Proton Conductance ◽  
Ampa Receptor Channels

1. The ionic mechanism of extracellular alkaline transients mediated by alpha-amino-3-hydroxy-5-methylisoxazolate-4-propionic acid (AMPA) receptor channels was studied in the rat hippocampal slice (area CA1) by means of H(+)-, Ca(2+)- and Na(+)-selective microelectrodes. 2. Alkaline transients mediated by selective synaptic activation of AMPA receptors were coupled to a transient fall in extracellular Ca2+ ([Ca2+]o). 3. Removal of [Ca2+]o blocked the alkaline transient evoked by microinjection of AMPA, but had little effect on the simultaneous tetrodotoxin-resistant fall in extracellular Na+ ([Na+]o). 4. Alkaline transients evoked by microinjection of gamma-aminobutyric acid (GABA) were not affected by removal of [Ca2+]o. GABA had no effect on [Na+]o. 5. The present results indicate that activity-induced glutamatergic alkaline transients in the hippocampal slice are due to an influx of Ca2+, and not to a conductive movement of H+ ions across glutamate-gated cation channels.

Glutamate receptors in dysplasic cortex: an in situ hybridization and immunohistochemistry study in rats with prenatal treatment with methylazoxymethanol

1998 ◽  
Vol 782 (1-2) ◽  
pp. 142-152 ◽  
Author(s):  
A Rafiki ◽  
N Chevassus-au-Louis ◽  
Y Ben-Ari ◽  
M Khrestchatisky ◽  
A Represa
Keyword(s):  
In Situ Hybridization ◽  
Glutamate Receptors ◽  
Prenatal Treatment
Sign in / Sign up

Export Citation Format

Share Document