Distribution of freshwater sponges in Serbia

2020 ◽  
Vol 193 (3) ◽  
pp. 195-204
Author(s):  
Stefan Andjus ◽  
Vladimir Lazović ◽  
Nadja Nikolić ◽  
Bojana Tubić ◽  
Vera Nikolić ◽  
...  

As data on the distribution of freshwater sponges (Porifera, Demospongiae, Spongillida) in Serbia are extremely scarce, we investigated the main Serbian rivers and lakes with respect to Porifera occurrence, for which 17 lotic and 11 lentic water bodies were selected. Sponges were found in 11 of 17 rivers (62 specimens in total) and in 3 of 11 lakes/reservoirs (seven specimens in total). Classical morphological spicule analysis was coupled with polymerase chain reaction (PCR) and gene sequencing for species identification. Among the 69 collected speci- mens, five sponge species of the family Spongillidae have been identified: Ephydatia fluviatilis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Ephydatia muelleri (Lieberkühn, 1856), Trochospongilla horrida Weltner, 1893, and Eunapius fragilis (Leidy, 1851). The most frequently found sponge in Serbian rivers was E. fluviatilis (45% of all specimens), while the least frequent was E. fragilis (6 % of all specimens). The Tisa river has the highest sponge diversity (four species). In lentic water bodies, only E. fluviatilis (four specimens) and S. lacustris (three specimens) were found. In general, sponges were infrequent and their abundance was low in Serbian fresh waters. While sponges seem to tolerate significant variations of physical and chemical parameters, some optimal values can be established.

2011 ◽  
Vol 140 (1) ◽  
pp. 74-77 ◽  
Author(s):  
S. K. DEY ◽  
O. PHATHAMMAVONG ◽  
T. D. NGUYEN ◽  
A. THONGPRACHUM ◽  
W. CHAN-IT ◽  
...  

SUMMARYSapovirus, a member of the family Caliciviridae, is one of the major causative agents of viral gastroenteritis affecting all age groups. A total of 3232 faecal specimens collected from infants and children with gastroenteritis in five different regions of Japan during 2003–2009 were examined for sapovirus by reverse transcription–polymerase chain reaction. Sapoviruses were detected in 131 (4·05%) patients with the peak observed mainly in the cold season (November–March) in Japan during 2003–2009. During the last 6 years, sapovirus GI/1 was the predominant strain in Japan followed by GIV, GII/3, GII/6, GII/2, GII/12 and GI, respectively.


Plant Disease ◽  
2013 ◽  
Vol 97 (2) ◽  
pp. 168-182 ◽  
Author(s):  
Robert R. Martin ◽  
Stuart MacFarlane ◽  
Sead Sabanadzovic ◽  
Diego Quito ◽  
Bindu Poudel ◽  
...  

Blackberry and raspberry are members of the family Rosaceae. They are classified in the genus Rubus, which comprises hundreds of species and has a center of origin in the Far East. Rubus is divided into 15 subgenera with blackberries classified in the Rubus (formerly Eubatus) and raspberries in the Idaeobatus subgenera. Rubus species are propagated vegetatively and are subject to infection by viruses during development, propagation, and fruit production stages. Reports of initial detection and symptoms of more than 30 viruses, virus-like diseases, and phytoplasmas affecting Rubus spp. were reviewed more than 20 years ago. Since the last review on Rubus viruses, significant progress has been made in the molecular characterization of many of the viruses that infect Rubus spp. Currently, reverse transcription–polymerase chain reaction detection methods are available for most of the viruses known to infect Rubus. The goals of this article are to update the knowledge on previously characterized viruses of Rubus, highlight recently described viruses, review the virus-induced symptoms, describe the advances made in their detection, and discuss our knowledge about several virus complexes that cause serious diseases in Rubus. Virus complexes have been identified recently as the major cause of diseases in blackberries and raspberries.


PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2933 ◽  
Author(s):  
Hoseong Choi ◽  
Yeonhwa Jo ◽  
Ju-Yeon Yoon ◽  
Seung-Kook Choi ◽  
Won Kyong Cho

Viroids are the smallest infectious agents, and their genomes consist of a short single strand of RNA that does not encode any protein.Chrysanthemum stunt viroid(CSVd), a member of the familyPospiviroidae, causes chrysanthemum stunt disease. Here, we report the genomic variations of CSVd to understand the sequence variability of CSVd in different chrysanthemum cultivars. We randomly sampled 36 different chrysanthemum cultivars and examined the infection of CSVd in each cultivar by reverse transcription polymerase chain reaction (RT-PCR). Eleven cultivars were infected by CSVd. Cloning followed by Sanger sequencing successfully identified a total of 271 CSVd genomes derived from 12 plants from 11 cultivars. They were further classified into 105 CSVd variants. Each single chrysanthemum plant had a different set of CSVd variants. Moreover, different single plants from the same cultivar had different sets of CSVd variants but identical consensus genome sequences. A phylogenetic tree using 12 consensus genome sequences revealed three groups of CSVd genomes, while six different groups were defined by the phylogenetic analysis using 105 variants. Based on the consensus CSVd genome, by combining all variant sequences, we identified 99 single-nucleotide variations (SNVs) as well as three nucleotide positions showing high mutation rates. Although 99 SNVs were identified, most CSVd genomes in this study were derived from variant 1, which is identical to known CSVd SK1 showing pathogenicity.


2011 ◽  
Vol 4 (1) ◽  
pp. 6-11
Author(s):  
Sanjibm Das ◽  
Dhrubes Biswas ◽  
Suman Roy

Author(s):  
B. Henderson-Sellers ◽  
P. B. R. Archer

Author(s):  
E.E. Ooi ◽  
L.R. Petersen ◽  
D.J. Gubler

There are 29 registered alphaviruses belonging to the family Togaviridae, 16 of which are known to cause human infection. They are RNA viruses with global geographical distribution and complex transmission cycles between wild or domestic animals or birds and one or more mosquito species; humans are infected by mosquito bites. They cause a spectrum of clinical manifestations ranging from nonspecific febrile illness to acute encephalitis and death. Diagnosis of infection is made serologically by detection of IgM and IgG antibodies, virus isolation, and polymerase chain reaction, or by immunohistochemistry on tissue samples....


Author(s):  
Agnieszka Szlauer-Łukaszewska ◽  
Vladimir Pešić

We analysed the occurrence of ostracods in a small river, taking into account all the types of water bodies in the floodplain − these included helocrenes, oxbow lakes, and ponds, as well as the main river channel. The objective of the study was to investigate the variation in ostracod communities and identify those factors determining species distribution. The environmental factors considered were the type of water body, responsible for 17% of the variance, the physical and chemical water properties (29%), and the biotic and abiotic factors associated with the substrate type (23%). Among the factors associated with the substrate, sediment sorting, plant coverage and insolation were the most important. The ostracod fauna of the helocrenes differed from that of the other water bodies in the floodplain. In the water bodies of the Krąpiel valley and in the main river channel, 33 ostracod species were recorded, of which 26 were found in the main river channel. Refugia in the floodplain were the main source of the diversity and abundance of ostracods in the main river channel. The mean density in the main river channel was very low, at 330 indiv. m−2, while in the water bodies of the floodplain it was the greatest, reaching up to 5568 indiv. m−2.


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