scholarly journals Association of the Extended-Spectrum β-Lactamase Gene blaTLA-1 with a Novel ISCR Element, ISCR20

2010 ◽  
Vol 54 (9) ◽  
pp. 4026-4028 ◽  
Author(s):  
Beatrice Berçot ◽  
Laurent Poirel ◽  
Jesus Silva-Sanchez ◽  
Patrice Nordmann
Keyword(s):  
Amino Acid ◽  
Mexico City ◽  
Insertion Sequence ◽  
Amino Acid Identity ◽  
Gene Encoding ◽  
Acid Identity ◽  
Promoter Sequences ◽  
Extended Spectrum ◽  
Lactamase Gene

ABSTRACT The bla TLA-1 gene encoding an extended-spectrum β-lactamase was identified in 11 enterobacterial isolates from Mexico City, Mexico. This gene was located on different plasmids and plasmid types with different sizes and incompatibility groups. It was associated with a novel insertion sequence, ISCR20, encoding a putative transposase that shared only 20% amino acid identity with the most closely related transposase of ISCR1. The ISCR20 element provided specific promoter sequences for expression of the bla TLA-1 gene.

scholarly journals Staphylococcus caprae Strains Carry Determinants Known To Be Involved in Pathogenicity: a Gene Encoding an Autolysin-Binding Fibronectin and the ica Operon Involved in Biofilm Formation

2001 ◽  
Vol 69 (2) ◽  
pp. 712-718 ◽  
Author(s):  
Jeanine Allignet ◽  
Sylvie Aubert ◽  
Keith G. H. Dyke ◽  
Nevine El Solh
Keyword(s):  
Amino Acid ◽  
Biofilm Formation ◽  
Insertion Sequence ◽  
Mobile Element ◽  
Amino Acid Identity ◽  
Gene Encoding ◽  
Surface Anchoring ◽  
Acid Identity ◽  
Putative Signal Peptide ◽  
Fibronectin Binding

ABSTRACT The atlC gene (1,485 bp), encoding an autolysin which binds fibronectin, and the ica operon, involved in biofilm formation, were isolated from the chromosome of an infectious isolate of Staphylococcus caprae and sequenced. AtlC (155 kDa) is similar to the staphylococcal autolysins Atl, AtlE, Aas (48 to 72% amino acid identity) and contains a putative signal peptide of 29 amino acids and two enzymatic centers (N-acetylmuramoyl-l-alanine amidase and endo-β-N-acetylglucosaminidase) interconnected by three imperfect fibronectin-binding repeats. The glycine-tryptophan (GW) motif found in the central and end part of each repeat may serve for cell surface anchoring of AtlC as they do in Listeria monocytogenes. The S. caprae ica operon contains four genes closely related to S. epidermidis and S. aureus icaA, icaB, icaC, and icaDgenes (≥ 68% similarity) and is preceded by a gene similar toicaR (≥70% similarity). The polypeptides deduced from theS. caprae ica genes exhibit 67 to 88% amino acid identity to those of S. epidermidis and S. aureus icagenes. The ica operon and icaR gene were analyzed in 14 S. caprae strains from human specimens or goats' milk. Some of the strains produced biofilm, and others did not. All strains carry the ica operon and icaR of the same sizes and in the same relative positions, suggesting that the absence of biofilm formation is not related to the insertion of a mobile element such as an insertion sequence or a transposon.

scholarly journals Chromosome-Encoded Ambler Class A β-Lactamase of Kluyvera georgiana, a Probable Progenitor of a Subgroup of CTX-M Extended-Spectrum β-Lactamases

2002 ◽  
Vol 46 (12) ◽  
pp. 4038-4040 ◽  
Author(s):  
Laurent Poirel ◽  
Peter Kämpfer ◽  
Patrice Nordmann
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Reference Strain ◽  
Amino Acid Identity ◽  
Acid Identity ◽  
Class A ◽  
Extended Spectrum ◽  
Lactamase Gene

ABSTRACT A chromosome-encoded β-lactamase gene, cloned and expressed in Escherichia coli from Kluyvera georgiana reference strain CUETM 4246-74 (DSM 9408), encoded the extended-spectrum β-lactamase KLUG-1, which shared 99% amino acid identity with the plasmid-mediated β-lactamase CTX-M-8. This work provides further evidence that Kluyvera spp. may be the progenitor(s) of CTX-M-type β-lactamases.

scholarly journals Characterization of a Chromosomally Encoded Extended-Spectrum Class A β-Lactamase from Kluyvera cryocrescens

2001 ◽  
Vol 45 (12) ◽  
pp. 3595-3598 ◽  
Author(s):  
Jean W. Decousser ◽  
Laurent Poirel ◽  
Patrice Nordmann
Keyword(s):  
Amino Acid ◽  
Reference Strain ◽  
Amino Acid Identity ◽  
Acid Identity ◽  
Class A ◽  
Extended Spectrum ◽  
Kluyvera Ascorbata ◽  
Lactamase Gene ◽  
Kluyvera Cryocrescens

ABSTRACT A chromosomally located β-lactamase gene, cloned and expressed inEscherichia coli from a reference strain of the enterobacterial species Kluyvera cryocrescens, encoded a clavulanic acid-inhibited Ambler class A enzyme, KLUC-1, with a pI value of 7.4. KLUC-1 shared 86% amino acid identity with a subgroup of plasmid-mediated CTX-M-type extended-spectrum β-lactamases (CTX-M-1, -3, -10, -11, and -12), the most closely related enzymes, and 77% amino acid identity with KLUA-1 from Kluyvera ascorbata.The substrate profile of KLUC-1 corresponded to that of CTX-M-type enzymes.

scholarly journals PER-6, an Extended-Spectrum β-Lactamase from Aeromonas allosaccharophila

2010 ◽  
Vol 54 (4) ◽  
pp. 1619-1622 ◽  
Author(s):  
Delphine Girlich ◽  
Laurent Poirel ◽  
Patrice Nordmann
Keyword(s):  
Amino Acid ◽  
Amino Acid Identity ◽  
Environmental Isolate ◽  
Kinetic Properties ◽  
Seine River ◽  
Acid Identity ◽  
Extended Spectrum

ABSTRACT An Aeromonas allosaccharophila environmental isolate recovered from the Seine River (Paris, France) produced a novel extended-spectrum β-lactamase, PER-6, that shared 92% amino acid identity with the closest ß-lactamase, PER-2. The kinetic properties of PER-6 showed a slightly increased affinity for carbapenems. The bla PER-6 gene was chromosomally located and bracketed by non-transposon-related structures.

scholarly journals Biochemical and Genetic Characterization of the vanC-2 Vancomycin Resistance Gene Cluster of Enterococcus casseliflavus ATCC 25788

2002 ◽  
Vol 46 (10) ◽  
pp. 3125-3132 ◽  
Author(s):  
Ireena Dutta ◽  
Peter E. Reynolds
Keyword(s):  
Amino Acid ◽  
Gene Cluster ◽  
Response Regulator ◽  
Extended Period ◽  
Amino Acid Identity ◽  
Serine Racemase ◽  
Gene Encoding ◽  
Acid Identity ◽  
Induction Process ◽  
Enterococcus Casseliflavus

ABSTRACT The vanC-2 cluster of Enterococcus casseliflavus ATCC 25788 consisted of five genes (vanC-2, vanXYC-2 , vanTC-2 , vanRC-2 , and vanSC-2 ) and shared the same organization as the vanC cluster of E. gallinarum BM4174. The proteins encoded by these genes displayed a high degree of amino acid identity to the proteins encoded within the vanC gene cluster. The putative d,d-dipeptidase-d,d-carboxypeptidase, VanXYC-2, exhibited 81% amino acid identity to VanXYC, and VanTC-2 displayed 65% amino acid identity to the serine racemase, VanT. VanRC-2 and VanSC-2 displayed high degrees of identity to VanRC and VanSC, respectively, and contained the conserved residues identified as important to their function as a response regulator and histidine kinase, respectively. Resistance to vancomycin was expressed inducibly in E. casseliflavus ATCC 25788 and required an extended period of induction. Analysis of peptidoglycan precursors revealed that UDP-N-acetylmuramyl-l-Ala-δ-d-Glu-l-Lys-d-Ala-d-Ser could not be detected until several hours after the addition of vancomycin, and its appearance coincided with the resumption of growth. The introduction of additional copies of the vanTC-2 gene, encoding a putative serine racemase, and the presence of supplementary d-serine in the growth medium both significantly reduced the period before growth resumed after addition of vancomycin. This suggested that the availability of d-serine plays an important role in the induction process.

scholarly journals Chromosome-Encoded Ambler Class D β-Lactamase of Shewanella oneidensis as a Progenitor of Carbapenem-Hydrolyzing Oxacillinase

2004 ◽  
Vol 48 (1) ◽  
pp. 348-351 ◽  
Author(s):  
Laurent Poirel ◽  
Claire Héritier ◽  
Patrice Nordmann
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Klebsiella Pneumoniae ◽  
Reference Strain ◽  
Shewanella Oneidensis ◽  
Amino Acid Identity ◽  
Gram Negative ◽  
Acid Identity ◽  
Class D ◽  
Lactamase Gene

ABSTRACT A chromosome-encoded β-lactamase gene from a Shewanella oneidensis reference strain was cloned and expressed in Escherichia coli. It encoded a carbapenem-hydrolyzing Ambler class D β-lactamase, OXA-54, that shared 92% amino acid identity with the plasmid-encoded carbapenem-hydrolyzing oxacillinase OXA-48 from Klebsiella pneumoniae. This work suggests that Shewanella spp. may produce the progenitor of oxacillinases compromising the efficacy of imipenem in clinically relevant gram-negative pathogens.

scholarly journals CTX-M-5, a Novel Cefotaxime-Hydrolyzing β-Lactamase from an Outbreak of Salmonella typhimuriumin Latvia

1998 ◽  
Vol 42 (8) ◽  
pp. 1980-1984 ◽  
Author(s):  
Patricia A. Bradford ◽  
Youjun Yang ◽  
Daniel Sahm ◽  
Ilze Grope ◽  
Dace Gardovska ◽  
...  
Keyword(s):  
Amino Acid ◽  
Salmonella Typhimurium ◽  
Nucleotide Sequencing ◽  
Amino Acid Substitutions ◽  
Gene Encoding ◽  
Extended Spectrum ◽  
Cephalosporin Resistance ◽  
Resistant Strains ◽  
Lactamase Gene ◽  
Gene Expressing

ABSTRACT At a children’s hospital in Riga, Latvia, isolates identified asSalmonella typhimurium were found to be resistant to expanded-spectrum cephalosporins. Two of the resistant strains were analyzed for the mechanism of cephalosporin resistance. Isoelectric focusing revealed a common β-lactamase with a pI of 8.8. In addition, one of the strains produced a pI 7.6 β-lactamase. A transconjugant producing only the pI 7.6 enzyme was susceptible to expanded-spectrum cephalosporins; therefore, this enzyme was most likely SHV-1. Transformants producing only the pI 8.8 β-lactamase were resistant to cefotaxime and aztreonam but were susceptible or intermediate to ceftazidime. A substrate profile determined spectrophotometrically with purified enzyme revealed potent activity against cefotaxime, with a relative k cat value of 95 (benzylpenicillin equal to 100). The enzyme showed lower relativek cat values for ceftazidime (3.3) and aztreonam (9.3). In addition, the enzyme was inhibited by clavulanate, sulbactam and tazobactam, with 50% inhibitory concentrations of 19, 100, and 3.4 nM, respectively. These results indicated the presence of an unusual extended-spectrum β-lactamase. The gene expressing the pI 8.8 β-lactamase was cloned. Nucleotide sequencing revealed a β-lactamase gene that differs from the gene encoding CTX-M-2, which also originated from S. typhimurium, by 11 nucleotides, 4 of which result in amino acid substitutions: Ala27Thr, Val230Gly, Glu254Ala, and Ile278Val. These results indicated the presence of a novel extended-spectrum β-lactamase, designated CTX-M-5, that specifically confers resistance to cefotaxime.

scholarly journals Coproduction of 16S rRNA Methyltransferase RmtD or RmtG with KPC-2 and CTX-M Group Extended-Spectrum β-Lactamases in Klebsiella pneumoniae

2013 ◽  
Vol 57 (5) ◽  
pp. 2397-2400 ◽  
Author(s):  
Maria Fernanda C. Bueno ◽  
Gabriela R. Francisco ◽  
Jessica A. O'Hara ◽  
Doroti de Oliveira Garcia ◽  
Yohei Doi
Keyword(s):  
Amino Acid ◽  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Amino Acid Identity ◽  
Aminoglycoside Resistance ◽  
Content Type ◽  
Acid Identity ◽  
Paulo State ◽  
Extended Spectrum ◽  
High Level

ABSTRACTEightKlebsiella pneumoniaeclinical strains with high-level aminoglycoside resistance were collected from eight hospitals in São Paulo State, Brazil, in 2010 and 2011. Three of them produced an RmtD group 16S rRNA methyltransferase, RmtD1 or RmtD2. Five strains were found to produce a novel 16S rRNA methyltransferase, designated RmtG, which shared 57 to 58% amino acid identity with RmtD1 and RmtD2. Seven strains coproduced KPC-2 with or without various CTX-M group extended-spectrum β-lactamases, while the remaining strain coproduced CTX-M-2.

scholarly journals Identification of a Progenitor of the CTX-M-9 Group of Extended-Spectrum β-Lactamases from Kluyvera georgiana Isolated in Guyana

2005 ◽  
Vol 49 (5) ◽  
pp. 2112-2115 ◽  
Author(s):  
Adam B. Olson ◽  
M. Silverman ◽  
David A. Boyd ◽  
Allison McGeer ◽  
Barbara M. Willey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Chromosomal Region ◽  
Amino Acid Identity ◽  
Nucleotide Identity ◽  
Acid Identity ◽  
Extended Spectrum ◽  
M 14

ABSTRACT Chromosomal β-lactamase genes (bla KLUY) from six Kluyvera georgiana strains isolated in Guyana were cloned and expressed in Escherichia coli. KLUY-1 exhibited 100% amino acid identity with the extended-spectrum β-lactamase CTX-M-14. We also show that a 2.7-kb Kluyvera chromosomal region exhibits 99% nucleotide identity to a portion of In60 that includes bla CTX-M-9.

scholarly journals Biochemical-Genetic Characterization and Distribution of OXA-22, a Chromosomal and Inducible Class D β-Lactamase fromRalstonia (Pseudomonas)pickettii

2000 ◽  
Vol 44 (8) ◽  
pp. 2201-2204 ◽  
Author(s):  
Patrice Nordmann ◽  
Laurent Poirel ◽  
Maryline Kubina ◽  
Anne Casetta ◽  
Thierry Naas
Keyword(s):  
Amino Acid ◽  
Genomic Dna ◽  
Genetic Characterization ◽  
Amino Acid Identity ◽  
Clinical Isolates ◽  
Acid Identity ◽  
Ralstonia Pickettii ◽  
Class D ◽  
Biochemical Genetic ◽  
Lactamase Gene

ABSTRACT From genomic DNA of Ralstonia pickettii isolate PIC-1, a β-lactamase gene was cloned that encodes the oxacillinase OXA-22. It differs from known oxacillinases, being most closely related to OXA-9 (38% amino acid identity). The hydrolytic spectrum of OXA-22 is limited mostly to benzylpenicillin, cloxacillin, and restricted-spectrum cephalosporins. OXA-22-like genes were identified as single chromosomal copies in five other R. pickettii clinical isolates. The expression of OXA-22-like β-lactamases was inducible in R. pickettii.

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