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2022 ◽  
Vol 8 ◽  
Author(s):  
Shuzhen Xiao ◽  
Chenyue Tang ◽  
Qian Zeng ◽  
Yilun Xue ◽  
Qing Chen ◽  
...  

Background: Bloodstream infections are recognized as important nosocomial infections. Escherichia coli (E. coli) is the most prevalent Gram-negative bacillary pathogen causing bloodstream infections (BSIs). This retrospective study investigated drug susceptibility and molecular epidemiology of E. coli isolated from patients with BSI in Shanghai, China.Methods: We collected E. coli isolated from the blood cultures of patients with BSI between January 2016 and December 2019. We randomly selected 20 strains each year to investigate antimicrobial resistance, resistance genes, and molecular epidemiological characteristics. Antimicrobial susceptibility testing was performed by the disk diffusion method. PCR was performed to detect extended-spectrum β-lactamases (ESBLs), carbapenemase genes, and housekeeping genes, and phyloviz was applied to analyze multilocus sequence typing (MLST).Results: Penicillins, first- and second-generation cephalosporins and fluoroquinolones have high resistance rates (>60%). Among the 80 randomly selected strains, 47 (58.8%) produced ESBLs, and one produced carbapenemase. Sequencing of resistance genes identified blaCTX−M−14 (34%, 16/47), blaCTX−M−15 (23.4%, 11/47) and blaCTX−M−27 (14.8%, 7/47) as the most prevalent genotypes of ESBLs. ST131 (14/80) was the most prevalent sequence type (ST), followed by ST1193 (10/80), ST648 (7/80).Conclusions: Our findings suggest that amikacin, carbapenems, and piperacillin-tazobactam have relatively low resistance rates and may be the preferred antibiotic regimens for empiric therapy. ST131 and blaCTX−M−14 are still the main prevalent in Shanghai with a rapid increase in the occurrence of ST1193 is rapidly increasing and more diverse blaCTX genes.


Author(s):  
Victor H. Duque ◽  
Pedro Sáenz-López ◽  
Miguel Ángel Gómez-Ruano ◽  
Sergio J. Ibáñez-Godoy ◽  
Cristina Conde ◽  
...  

In spite of the negative effects of anger, coaches are often seen becoming angry during games. This is especially worrying in U18 categories. Thus, the objective of this study was to identify the influence that the coach’s anger has on the performance of a basketball team in competition. For this, an ad hoc observation tool was designed, in which 587 moments of anger from the coaching staff (64 coaches) were recorded in the 24 semi-final and final matches of the Spanish Autonomous Region Team Championships in 2019 and 2020 in the infantil (M = 14 years old) and cadete (M = 16 years old) categories. The results show that, in response to most incidents of coach anger, the performance of the team did not change. Significant differences were identified in some scenarios, with low- or medium-intensity anger targeted at the defence, where the team performance improved. However, anger towards the referee in the last quarter with scores level had a negative influence on the team’s performance.


2021 ◽  
Vol 78 (4) ◽  
pp. 384-390
Author(s):  
Evelin Bachmeier ◽  
María Elena Migueles Goitea ◽  
Jorge Alberto Linares ◽  
Fernando Martin Wietz ◽  
Sol Jarchum ◽  
...  

OBJETIVO: altas dosis de quimioterapia utilizadas previo al trasplante de médula ósea (TMO) pueden promover severos cambios en el sistema estomatognático. El objetivo consistió en evaluar algunos marcadores funcionales, inmunológicos y de estrés oxidativo en saliva de pacientes sometidos a dicho tratamiento. MÉTODOS: estudio observacional longitudinal en 22 pacientes de la Unidad de trasplante de Médula Ósea del Servicio de Oncohematología del Sanatorio Allende. Se efectuó recolección de saliva basal en etapa inicial (I) previa al aislamiento y etapa media (M) 14 días posteriores a la terapia de acondicionamiento y trasplante. Se analizó la concentración de ácido úrico (AU), superóxido dismutasa (SOD), malondialdehido (MDA), alfa amilasa salival, inmunoglobulina A secretora (Ig As), lactoferrina, ceruloplasmina y urea. RESULTADOS: en (M) los niveles de SOD y MAD aumentaron significativamente respecto de (I) (p< 0.01). La concentración de  alfa amilasa salival, Ig As, lactoferrina y ácido úrico fue significativamente menor en (M) respecto de ( I ) p < 0.0001, p < 0.01, p < 0.0001, p <0.02 respectivamente. Ceruloplasmina y Urea no mostraron variaciones. CONCLUSIÓN: se observó una disminución de la capacidad defensiva de la saliva como consecuencia de una reducción de la concentración de Ig As y lactoferrina. El incremento de SOD en (M) podría interpretarse como un mecanismo de defensa de la saliva contra el estrés oxidativo producido por la quimioterapia. La disminución de ácido úrico en la etapa (M) podría favorecer el agravamiento de mucositis.  La síntesis y liberación de amilasa fue afectada por el tratamiento con citostáticos.


2021 ◽  
Vol 34 ◽  
pp. 106-109
Author(s):  
G. Kokhirova ◽  
H. Relke ◽  
Q. Yuldoshev ◽  
Yu.I. Protsyuk ◽  
V.M. Andruk

In the Tycho-2 catalogue system the processing of 1529 photographic plates of the FON Dushanbe project from the collection of the Institute of Astrophysics of the National Academy of Sciences of Tajikistan was completed. The photographic plates with the size of 8º×8º (30x30 cm) were exposed in the zones from -8º to + 84º in the period of 1985-1992 years. In years 2017 – 2020 the plates were digitized using a Microtek ScanMaker 1000XL Plus scanner with the resolution of 1200 dpi, so the size of the digitized images is near 13000x13000 px. Based on the results of the processing of digitized images a catalogue of equatorial coordinates α, δ and B-magnitudes of stars for the northern hemisphere of the sky was created. The catalog contains about 30 million stars and galaxies for the epoch 1988.74. The average internal accuracy of the catalogue for all objects is σαδ = ±0.32" and σB = ± 0.11 m (for stars in the range of B = 8 m -14 m the errors are σαδ = ±0.19" and σB =±0.07 m ) for equatorial coordinates and B-magnitudes respectively. The convergence between calculated and reference positions from the Tycho-2 catalogue is σαδ = ±0.07" and the convergence with photoelectric B-magnitudes is σB = ±0.16 m . Five astronomical institutions took part in the processing of the photographic plates and in the creating of the FON-Dushanbe catalogue: Institute of Astrophysics of NAS of Tajikistan; Walter Hohmann Observatory, Essen, Germany; Ulugh Beg Astronomical Institute UAS, Uzbekistan; Research Institute “Mykolaiv Astronomical Observatory”, Ukraine and Main Astronomical Observatory NASU, Ukraine.


Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1463
Author(s):  
Toyotaka Sato ◽  
Shin-ichi Yokota ◽  
Tooru Tachibana ◽  
Satoshi Tamai ◽  
Shigeki Maetani ◽  
...  

An increase in human and veterinary fluoroquinolone-resistant Escherichia coli is a global concern. In this study, we isolated fluoroquinolone-resistant E. coli isolates from companion animals and characterized them using molecular epidemiological analysis, multiplex polymerase chain reaction to detect E. coli ST131 and CTX-M type extended-spectrum β-lactamases (ESBL), and multi-locus sequence typing analysis. Using plain-CHROMagar ECC, 101 E. coli isolates were isolated from 34 rectal swabs of dogs and cats. The prevalence of resistance to fluoroquinolone and cefotaxime was 27.7% and 24.8%, respectively. The prevalence of fluoroquinolone-resistant isolates (89.3%) was higher when CHROMagar ECC with CHROMagar ESBL supplement was used for E. coli isolation. The prevalence of cefotaxime resistance was also higher (76.1%) when 1 mg/L of ciprofloxacin-containing CHROMagar ECC was used for isolation. The cefotaxime-resistant isolates possessed CTX-M type β-lactamase genes (CTX-M-14, CTX-M-15, or CTX-M-27). Seventy-five percent of fluoroquinolone-resistant isolates were sequence types ST131, ST10, ST1193, ST38, or ST648, which are associated with extensive spread in human clinical settings. In addition, we isolated three common fluoroquinolone-resistant E. coli lineages (ST131 clade C1-M-27, C1-nM27 and ST2380) from dogs and their respective owners. These observations suggest that companion animals can harbor fluoroquinolone-resistant and/or ESBL-producing E. coli, in their rectums, and that transmission of these isolates to their owners can occur.


2021 ◽  
Vol 12 ◽  
Author(s):  
Qiucheng Shi ◽  
Yihua Ye ◽  
Peng Lan ◽  
Xinhong Han ◽  
Jingjing Quan ◽  
...  

The non-Typhi Salmonella (NTS) infection is critical to children’s health, and the ceftriaxone is the important empirical treatment choice. With the increase resistance rate of ceftriaxone in Salmonella, the molecular epidemiology and resistance mechanism of ceftriaxone-resistant Salmonella needs to be studied. From July 2019 to July 2020, a total of 205 NTS isolates were collected, 195 of which (95.1%) were cultured from stool, but 10 isolates were isolated from an extraintestinal site. Serogroup B accounted for the vast majority (137/205) among the isolates. Fifty-three isolates were resistant to ceftriaxone, and 50 were isolated from children younger than 4years of age. The resistance rates for ceftriaxone, ciprofloxacin, and levofloxacin were significantly higher in younger children than the older children. The resistance genes in the ceftriaxone-susceptible isolates were detected by PCR, and ceftriaxone-resistant Salmonella were selected for further whole-genome sequencing. Whole-genome analysis showed that serotype Typhimurium and its monophasic variant was the most prevalent in ceftriaxone-resistant isolates (37/53), which comprised ST34 (33/53), ST19 (2/53), and ST99 (2/53), and they were close related in the phylogenetic tree. However, the other isolates were diverse, which included one Enteritidis (ST11), one Indiana (ST17), one Derby (ST40), four Kentucky (ST198), two Goldcoast (ST2529, ST358), one Muenster (ST321), one Virchow (ST359), one Rissen (ST469), one Kedougou (ST1543), two Uganda (ST684), and one Kottbus (ST8839). Moreover, CTX-M-55 ESBLs production (33/53) was found to be mainly responsible for ceftriaxone resistance, followed by blaCTX-M-65 (12/53), blaCTX-M-14 (4/53), blaCTX-M-9 (2/53), blaCTX-M-64 (1/53), blaCTX-M-130 (1/53), and blaCMY-2 (1/53). ISEcp1, IS903B, IS Kpn26, IS1F, and IS26 were connected to antimicrobial resistance genes transfer. In conclusion, the dissemination of ESBL-producing Salmonella isolates resulted in an increased prevalence of ceftriaxone resistance in young children. The high rate of multidrug resistance should be given additional attention.


Circulation ◽  
2021 ◽  
Vol 144 (Suppl_2) ◽  
Author(s):  
Nanna B Christensen ◽  
Fredrik Folke ◽  
Louise Kollander Jakobsen ◽  
Anne J Jørgensen ◽  
Julie Kjoelbye ◽  
...  

Introduction: High density of citizen responders (CR) and automated external defibrillators (AEDs) may increase chances for early bystander defibrillation in out-of-hospital cardiac arrest (OHCA). We aimed to assess coverage using current CR and AED positions applied on historical OHCAs in Denmark. Methods: Non-EMS witnessed OHCAs from the Danish cardiac arrest registry with known location (2016-2019) and AEDs registered with the Danish AED network (November 2020) were included. Locations of all CRs registered with the national CR program were identified (Wed, December 2, 2020) at 12pm (noon) and 12am (midnight). Since pilot data showed 25% of alerted CRs accepted the alarm, we investigated OHCA CR coverage defined ≥4 CR within <1800m (1969 yd), <500m (547 yd) and <200m (219 yd), and OHCA CR + AED coverage as ≥4 CR and 1 AED <1800m, <500m and <200m. We compared OHCA coverage during daytime and nighttime. Results: A total of 18,128 OHCAs (median age 73 years, 63.4% male) were included. A total of 22,418 AEDs (386/100,000 inhabitants) were available at 12pm, 65% were accessible 24/7. A total of 34,033 CR (586 CR/100,000 inhabitants) were available at 12am and 33,938 were available at12pm. During daytime, a median of 29 AEDs and 37 CRs were <1800m of historical OHCAs. Most OHCAs were covered by CRs and AEDs <1800m decreasing with shorter distances with little difference according to time of day (Figure 1). Conclusion: Following the implementation of a nationwide AED network and a citizen responder program, most historical OHCAs (85%) were < 1800m of CRs and AEDs at midnight with a slight decrease during daytime (82%). A decrease in CR and AED coverage were observed for 500m (59%) and 200 m (14%), with little difference according to time of day. During daytime a median of 29 AEDs and 37 CRs were < 1800m of historical OHCAs. Our results indicate successful implementation of a national AED registry and CR program with great potential for improving bystander defibrillation.


2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S704-S705
Author(s):  
Alyssa K Whitney ◽  
Nancy D Hanson

Abstract Background K. pneumoniae can emerge resistant to β-lactam antibiotics through the production of β-lactamase enzymes and/or loss of the outer membrane porins, OmpK35, OmpK36, and/or PhoE. While both mechanisms are hypothesized to work synergistically, β-lactamases have been the focus of previous studies. As a result, the contribution of outer membrane porin loss to the β-lactam minimum inhibitory concentration (MIC) is unknown. The objective of this study was to evaluate the contribution of specific β-lactamases and porin production to β-lactam susceptibility. We hypothesize that production of a β-lactamase in a clinical isolate deficient in 3 major porins will result in higher β-lactam MICs but not always a resistant phenotype. Methods The structural gene and promoter of CTX-M-14, CTX-M-15, and CMY-2 were cloned into a low copy number vector and transformed into Kp 23, a wild-type clinical isolate, and KPM 20, a clinical isolate deficient in OmpK35/36 and PhoE. MICs to ceftolozane/tazobactam, cefotaxime, ceftazidime, cefepime, and meropenem were determined by E-test. Kp 23 and KPM 20 were characterized by Western blot and whole genome sequencing. Results Production of CMY-2 alone led to a resistant phenotype for ceftolozane/tazobactam, cefotaxime, and ceftazidime regardless of porin production (Figure 1). CMY-2 production in KPM 20 resulted in non-susceptibility to meropenem. Both clones were susceptible to cefepime. Production of CTX-M-14 and CTX-M-15 in Kp 23 resulted in only cefotaxime resistance. Production of CTX-M-14 and CTX-M-15 in KPM 20 resulted in isolates non-susceptible to all antibiotics tested. Figure 1. MICs of K. pneumoniae clones against panel of β-lactam antibiotics. Conclusion When evaluating clinical isolates, it is impossible to determine the contribution of individual resistance mechanisms in the susceptibility pattern. This study demonstrated that resistance is not solely dependent on the β-lactamase produced and that the impact of porin deficiency varies with the antibiotic being evaluated. These data suggest that antibiotic selection may be more nuanced and that a broader range of therapeutics may be available given the appropriate diagnostic tools. Understanding the contributions of all resistance mechanisms is necessary to inform selection of the most appropriate antibiotic therapy. Disclosures Nancy D. Hanson, PhD, Merck (Grant/Research Support)


Author(s):  
Max Supke ◽  
Wolfgang Schulz
Keyword(s):  

Zusammenfassung. Ziele: In der vorliegenden Studie werden sowohl frühkindliche als auch elterliche Risikofaktoren, die jugendliche Trinkmotivation und die Übereinstimmung zwischen den Müttern und Jugendlichen hinsichtlich des jugendlichen Alkoholkonsums zur Ableitung von präventiven Ideen ausgewertet. Methodik: Die Daten von 239 Familien aus dem längsschnittlichen deutschen Projekt Zukunft Familie wurden vom Kindergartenalter ( M = 4.5 Jahre) bis in das Jugendalter ( M = 14 Jahre) erhoben. Mittels binär logistischer Regressionsmodelle wurden die Zusammenhänge zwischen psychischen Auffälligkeiten im Kindesalter, der soziodemographischen Situation sowie Verhaltensweisen der Eltern und dem Alkoholkonsum der Kinder zehn Jahre später analysiert. Ergebnisse: Kinder von Müttern mit einer höheren Schulbildung und aus Familien mit einem höheren sozialen Status hatten ein erhöhtes Risiko, schon vor dem gesetzlich erlaubten Mindestalter, regelmäßig Alkohol zu trinken. Bei den im Durchschnitt 4.5-jährigen Kindern erwiesen sich internalisierende Auffälligkeiten als Schutzfaktor vor einem frühzeitigen jugendlichen Konsum. Alkohol wurde im Jugendalter vor allem aus sozialen und Verstärkungsmotiven getrunken. Mütter unterschätzten signifikant den Konsum ihrer Kinder um das Vier- bis Fünffache. Schlussfolgerung: Präventionsmaßnahmen könnten den Jugendlichen Alternativen aufzeigen, um die sozialen und verstärkenden Motivationen auf risikofreien Wegen zu erreichen. Zur verbesserten Einschätzung des kindlichen Konsums, könnten Mütter in Hinweisen geschult werden, die auf einen übermäßigen Konsum hindeuten. Das erhöhte Risiko von Kindern aus Familien mit einem höheren sozialen Status könnte dabei berücksichtigt werden.


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