scholarly journals Rickettsia felis Infection in a Common Household Insect Pest, Liposcelis bostrychophila (Psocoptera: Liposcelidae)

2010 ◽  
Vol 76 (7) ◽  
pp. 2280-2285 ◽  
Author(s):  
Adi Behar ◽  
Laurie J. McCormick ◽  
Steve J. Perlman
Keyword(s):  
Molecular Characterization ◽  
Insect Pest ◽  
Blood Feeding ◽  
Human Pathogen ◽  
Rickettsia Felis ◽  
Liposcelis Bostrychophila ◽  
The One

ABSTRACT Many species of Rickettsia are well-known mammalian pathogens transmitted by blood-feeding arthropods. However, molecular surveys are continually uncovering novel Rickettsia species, often in unexpected hosts, including many arthropods that do not feed on blood. This study reports a systematic molecular characterization of a Rickettsia infecting the psocid Liposcelis bostrychophila (Psocoptera: Liposcelidae), a common and cosmopolitan household pest. Surprisingly, the psocid Rickettsia is shown to be Rickettsia felis, a human pathogen transmitted by fleas that causes serious morbidity and occasional mortality. The plasmid from the psocid R. felis was sequenced and was found to be virtually identical to the one in R. felis from fleas. As Liposcelis insects are often intimately associated with humans and other vertebrates, it is speculated that they acquired R. felis from fleas. Whether the R. felis in psocids causes disease in vertebrates is not known and warrants further study.

scholarly journals Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis

2009 ◽  
Vol 10 (1) ◽  
pp. 97 ◽  
Author(s):  
Hualiang He ◽  
Mei Cheng ◽  
Xiao Yang ◽  
Jinxiu Meng ◽  
Ai He ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Angiostrongylus Cantonensis ◽  
Human Pathogen

scholarly journals Molecular Analysis of Aquaglyceroporin 1 Gene in Non-Healing Clinical Iso¬lates Obtained from Patients with Cutaneous Leishmaniasis from Central of Iran

2019 ◽  
Author(s):  
Yasaman Alijani ◽  
Saeedeh Sadat Hosseini ◽  
Salman Ahmadian ◽  
Sonia Boughattas ◽  
Gilda Eslami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cutaneous Leishmaniasis ◽  
Molecular Characterization ◽  
Significance Level ◽  
Detection And Identification ◽  
Pcr Rflp ◽  
Leishmania Spp ◽  
Sensitive Isolate ◽  
The One

Background: Regarding the antimonial-resistant of Leishmania spp., understanding of related mechanism is neces­sary. One of the most important involved molecules is aquaglyceropin1 (AQP1). The aim of this study was molecu­lar analysis of AQP1 gene from antimonial-resistant clinical isolates and its expression. Methods: Overall, 150 patients with cutaneous leishmaniasis referring to the reference laboratories of Yazd and Varzaneh,, located 105km southeast of Isfahan and 240km away from Yazd, were assessed from Jun 2015 to Dec 2017. After sampling, staining was done and evaluated for Leishman by microscope. Samples were collected in RNAlater solution for gene expression analysis in non-healing isolates. DNA extraction was performed from each slide with Leishman body. All patients with L. major isolates detected by ITS1-PCR-RFLP were followed for find­ing the resistant isolates, consequence of molecular characterization of AQP1 using PCR-RFLP. Gene expression of AQP1 from all resistant isolates was assessed in comparison with the one in a sensitive isolate. Statistical analysis was done using SPSS. The significance level was considered ≤0.05. Results: Five isolates were detected as antimonial resistant. Molecular detection and identification were appeared that all were L. major. The molecular characterization of AQP1 showed G562A mutation. Gene expression of AQP1 in resistant isolates showed 1.67 fold higher than the sensitive isolate. Conclusion: We reported a new point mutation of G562A in AQP1 gene involved in molecular mechanism in re­sistant isolates.

scholarly journals Morphometric characterization of Rastrococcus invadens on four varieties of mango trees (Pout, Thies)

2021 ◽  
Vol 9 (3) ◽  
pp. 116-124
Author(s):  
Amadou Fall ◽  
Adiouma G.R. J. Sarr ◽  
Fawrou Seye ◽  
Mamour Toure ◽  
Raymond D. Ndione ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Insect Pest ◽  
Fruit Trees ◽  
The Other ◽  
Local Varieties ◽  
Improved Varieties ◽  
Rastrococcus Invadens ◽  
The One ◽  
Morphometric Characterization

Rastrococcus invadens (Homoptera, Pseudococcidae) or mealybug is an insect pest of mango and several other fruit trees including citrus. The cochineal is native to Southeast Asia and was first identified in Senegal in Dakar in 1995. Since then, it has been widely distributed throughout the country and more particularly in the two most fruit-producing areas: Casamance and Thies. To properly conduct our study, we chose the four most infested mango varieties namely Kent, Keitt, Sewe and Boukodiekhal. All these populations are from the locality of Pout (Thies region). On each plant we collected 10 people. This allowed us to have a total of 40 individuals. The individuals harvested are coded according to the zone and the type of plant in which they were harvested. This study revealed almost two more or less distinct morphometric groups made up on the one hand by populations from improved varieties (Kent and Keitt) and on the other by those from local varieties (Sewe and Boukodiekhal).

Molecular characterization of diverse defensins (γ-thionins) from Capsicum annuum flowers and their effects on the insect pest Helicoverpa armigera

Plant Gene ◽  
2021 ◽  
pp. 100284
Author(s):  
Javed A. Mulla ◽  
Anuja N. Kibe ◽  
Dhanashree D. Deore ◽  
Abhilash R. Jadhav ◽  
Vaijayanti A. Tamhane
Keyword(s):  
Molecular Characterization ◽  
Capsicum Annuum ◽  
Helicoverpa Armigera ◽  
Insect Pest ◽  
Helicoverpa Armígera

scholarly journals Expression Patterns, Molecular Characterization, and Response to Host Stress of CYP Genes from Phenacoccus solenopsis (Hemiptera: Pseudococcidae)

Insects ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 264
Author(s):  
Lingyu Xi ◽  
Dan Liu ◽  
Lei Ma ◽  
Ying Zhang ◽  
Ruirui Sheng ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Host Plants ◽  
Adult Stage ◽  
Insect Pest ◽  
Expression Patterns ◽  
Pcr Analysis ◽  
Broad Host Range ◽  
Phenacoccus Solenopsis ◽  
P450 Monooxygenase

The quarantine insect pest Phenacoccus solenopsis (Hemiptera: Pseudococcidae) has a broad host range and is distributed worldwide. Each year, P. solenopsis causes significant crop losses. The detoxification of various xenobiotic compounds involves the cytochrome P450 monooxygenase (CYP) superfamily of enzymes. However, the functions of CYPs in P. solenopsis are poorly understood. In the present study, P. solenopsis was reared from the egg to the adult stage on three host plants: Tomato, cotton, and hibiscus. Thirty-seven P. solenopsis CYP genes were identified and their phylogenetic relationships were analyzed. Eleven CYP genes (PsCYP4NT1, PsCYP4G219, PsCYP6PZ1, PsCYP6PZ5, PsCYP301B1, PsCYP302A1, PsCYP305A22, PsCYP315A1, PsCYP353F1, PsCYP3634A1, and PsCYP3635A2) were selected for quantitative real-time PCR analysis. The results demonstrated marked differences in CYP expression levels in P. solenopsis grown on different host plants. The results will aid the molecular characterization of CYPs and will increase our understanding of CYP expression patterns in P. solenopsis during development and growth on different hosts.

scholarly journals Rickettsia felis: molecular characterization of a new member of the spotted fever group.

2001 ◽  
Vol 51 (2) ◽  
pp. 339-347 ◽  
Author(s):  
D H Bouyer ◽  
J Stenos ◽  
P Crocquet-Valdes ◽  
C G Moron ◽  
V L Popov ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Spotted Fever ◽  
Spotted Fever Group ◽  
Rickettsia Felis

Molecular characterization of Staphylococcus aureus in Lebanon

2010 ◽  
Vol 138 (5) ◽  
pp. 707-712 ◽  
Author(s):  
S. T. TOKAJIAN ◽  
P. ABOU KHALIL ◽  
D. JABBOUR ◽  
M. RIZK ◽  
M. J. FARAH ◽  
...  
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Protein A ◽  
Multi Locus Sequence Typing ◽  
Health Concern ◽  
Human Pathogen ◽  
Public Health Concern ◽  
Sequence Types

SUMMARYStaphylococcus aureus is an important human pathogen and is a growing public health concern. In this study, 130 S. aureus, 93 methicillin-resistant S. aureus (MRSA) and 37 methicillin-sensitive S. aureus (MSSA), clinical isolates recovered from Lebanon were typed by protein A gene (spa) sequencing and multi-locus sequence typing (MLST). Forty-eight different spa types were identified and clustered into 30 different groups. MLST revealed 10 sequence types (STs) among the isolates. There were eight major MRSA clones defined as isolates with the same ST and the same SCCmec type. The majority of the PVL-positive isolates (53%) were ST80-MRSA-IVc. Systematic surveillance of both hospital and community isolates in Lebanon together with measures designed to limit the spread are required.

scholarly journals Molecular characterization of two novel deltamethrin-inducible P450 genes from Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae)

2010 ◽  
Vol 74 (1) ◽  
pp. 17-37 ◽  
Author(s):  
Hong-Bo Jiang ◽  
Pei-An Tang ◽  
Yong-Qiang Xu ◽  
Feng-Ming An ◽  
Jin-Jun Wang
Keyword(s):  
Molecular Characterization ◽  
Liposcelis Bostrychophila

scholarly journals Molecular Characterization of Equine Isolates ofStreptococcus pneumoniae: Natural Disruption of Genes Encoding the Virulence Factors Pneumolysin and Autolysin

1999 ◽  
Vol 67 (6) ◽  
pp. 2776-2782 ◽  
Author(s):  
Adrian M. Whatmore ◽  
Samantha J. King ◽  
Neil C. Doherty ◽  
Daniel Sturgeon ◽  
Neil Chanter ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Virulence Factor ◽  
Virulence Determinants ◽  
Human Pathogen ◽  
Phenotypic Data ◽  
Major Virulence Factor ◽  
Genes Encoding ◽  
Important Virulence Factor ◽  
Human Infections

ABSTRACT Although often considered a strict human pathogen,Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from horses in England and Ireland. Despite being obtained from geographically distinct locations, the isolates were found to represent a tight clonal group, virtually identical to each other but genetically distinguishable from more than 120 divergent isolates of human S. pneumoniae. A comprehensive analysis of known pneumococcal virulence determinants was undertaken in an attempt to understand the pathogenicity of equine pneumococci. Surprisingly, equine isolates appear to lack activities associated with both the hemolytic cytotoxin pneumolysin, often considered a major virulence factor of pneumococci, and the major autolysin gene lytA, also considered an important virulence factor. In support of phenotypic data, molecular studies demonstrated a deletion of parts of the coding sequences of both lytA and ply genes in equine pneumococci. The implications of these findings for the evolution and pathogenicity of equine S. pneumoniae are discussed.

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