Predominance of human bocavirus genotypes 1 and 2 in oysters in Thailand

Human bocavirus (HBoV) has been recognized as one of important pathogens of respiratory infection and acute gastroenteritis in young children worldwide. HBoV is most likely transmitted by the respiratory route and fecal-oral transmission. Recently, HBoV has been detected in several types of environmental water and bivalve shellfish. However, study of the existence of HBoV in oyster is still undocumented in Thailand. In this study, 144 oyster samples collected from different markets in Chiang Mai, Thailand during 2017 to 2018 were investigated for the presence of HBoV by nested-PCR and sequencing. HBoV was detected in 11 out of 144 samples (7.6%). Nine HBoV-positive samples (81.8%) were identified as genotype 1 (HBoV1) and two (18.2%) as HBoV2. A monthly investigation of HBoV in oyster samples from July 2017 to June 2018 showed that HBoV was sporadically detected in some particular months spanning the rainy and colder season with its peak in January. This study demonstrates the presence and genotype diversity of HBoV in oyster samples in Thailand. The finding contributes to evaluating the risk of foodborne transmission of HBoV and monitoring the outbreak of HBoV in Thailand and other countries. Importance Human bocavirus is recognized as an important cause of respiratory infection and of acute gastroenteritis in children worldwide. Human bocavirus is widely detected in many clinical specimens as well as in several environmental samples. Most of the studies describe the incidence of bocavirus infection in human while only few data are available for the occurrence of human bocavirus in food materials, particularly, in bivalve shellfish. Our findings provide the evidence for the existence and prevalence of human bocavirus in oysters suggesting that further monitoring the potential risk of food and waterborne transmission of this virus to human should be undertaken.

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Human bocavirus genotypes 1, 2, and 3 circulating in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand, 2012–2018

Journal of Infection and Public Health ◽

10.1016/j.jiph.2020.12.007 ◽

2021 ◽

Vol 14 (2) ◽

pp. 179-186

Author(s):

Nattika Nantachit ◽

Pakawat Kochjan ◽

Pattara Khamrin ◽

Kattareeya Kumthip ◽

Niwat Maneekarn

Keyword(s):

Acute Gastroenteritis ◽

Pediatric Patients ◽

Human Bocavirus ◽

Chiang Mai

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Identification of Human Bocavirus type 4 in a child asymptomatic for respiratory tract infection and acute gastroenteritis – Goiânia, Goiás, Brazil

The Brazilian Journal of Infectious Diseases ◽

10.1016/j.bjid.2017.03.012 ◽

2017 ◽

Vol 21 (4) ◽

pp. 472-476 ◽

Cited By ~ 2

Author(s):

Teresinha Teixeira de Sousa ◽

Tâmera Nunes Vieira Almeida ◽

Fabíola Souza Fiaccadori ◽

Menira Souza ◽

Kareem Rady Badr ◽

...

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Acute Gastroenteritis ◽

Human Bocavirus

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Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762012000600015 ◽

2012 ◽

Vol 107 (6) ◽

pp. 800-804 ◽

Cited By ~ 10

Author(s):

Teresinha Teixeira de Sousa ◽

Menira Souza ◽

Fabíola Souza Fiaccadori ◽

Ana Maria Tavares Borges ◽

Paulo Sucasas da Costa ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Human Bocavirus

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Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Applied and Environmental Microbiology ◽

10.1128/aem.02754-17 ◽

2018 ◽

Vol 84 (7) ◽

Cited By ~ 2

Author(s):

G. La Rosa ◽

G. Purpari ◽

A. Guercio ◽

S. Di Bella ◽

F. Gucciardi ◽

...

Keyword(s):

Respiratory Infections ◽

Pediatric Population ◽

Human Bocavirus ◽

Spatial Spread ◽

River Waters ◽

Italian Regions ◽

Significant Difference ◽

Tract Infections ◽

Production Areas ◽

Bivalve Shellfish

ABSTRACTHuman bocavirus (HBoV) has been shown to be a common cause of respiratory infections and gastroenteritis in children. Recently, HBoVs have been detected in sewage and river waters in Italy and worldwide. However, studies on their presence in other water environments and in bivalve mollusks are not yet available. In this study, 316 bivalve shellfish samples collected in three Italian regions over a 6-year period (2012 to 2017) were analyzed by nested PCR and sequencing using broad-range primer pairs targeting the capsid proteins VP1 and VP2 of HBoV. The virus was detected in 27 samples (8.5% of the total samples), and a statistically significant difference was found within the three regions. A further 13 samples, collected in geographic and temporal proximity to positive samples, were included in the study to assess the spread of HBoV in shellfish production areas at the time of contamination. Twelve of these additional samples were found to be positive for HBoV. All positive samples in this study were characterized as HBoV species 2 (17 samples; 8 different sequences) or species 3 (22 samples; 4 different sequences). This study reports the occurrence of HBoV in bivalve shellfish and shows evidence of considerable spatial spread of the virus throughout shellfish production areas. Further studies are needed to elucidate both the role of HBoV as an agent of gastroenteritis and the risk for foodborne transmission of this virus.IMPORTANCEHuman bocavirus is recognized as an important cause of acute respiratory tract infections and has recently been considered an etiological agent of gastroenteritis in the pediatric population. Our findings document that HBoVs are detected in bivalve shellfish with a relevant prevalence and suggest that an assessment of the risk for foodborne transmission of these viruses should be undertaken.

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Molecular epidemiology of human bocavirus infection in hospitalized children with acute gastroenteritis in South Africa, 2009‐2015

Journal of Medical Virology ◽

10.1002/jmv.25634 ◽

2019 ◽

Vol 92 (8) ◽

pp. 1124-1132 ◽

Cited By ~ 5

Author(s):

Rembuluwani Netshikweta ◽

Lizyben Chidamba ◽

Sandrama Nadan ◽

Maureen B. Taylor ◽

Nicola A. Page

Keyword(s):

South Africa ◽

Molecular Epidemiology ◽

Acute Gastroenteritis ◽

Human Bocavirus ◽

Hospitalized Children

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Phenotyping of Lewis and secretor HBGA from saliva and detection of new FUT2 gene SNPs from young children from the Amazon presenting acute gastroenteritis and respiratory infection

Infection Genetics and Evolution ◽

10.1016/j.meegid.2019.02.011 ◽

2019 ◽

Vol 70 ◽

pp. 61-66 ◽

Cited By ~ 5

Author(s):

Marcia Terezinha Baroni de Moraes ◽

Alberto Ignácio Olivares Olivares ◽

Alexandre Madi Fialho ◽

Fábio Correia Malta ◽

Sergio da Silva e Mouta Junior ◽

...

Keyword(s):

Young Children ◽

Respiratory Infection ◽

Acute Gastroenteritis ◽

Fut2 Gene

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Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis

Viruses ◽

10.3390/v10110639 ◽

2018 ◽

Vol 10 (11) ◽

pp. 639 ◽

Cited By ~ 3

Author(s):

Zaiga Nora-Krukle ◽

Anda Vilmane ◽

Man Xu ◽

Santa Rasa ◽

Inga Ziemele ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Human Bocavirus ◽

Plasma Samples ◽

Infectious Agents ◽

Viral Dna ◽

Widespread Distribution ◽

Gastrointestinal Pathogens ◽

Stool Samples ◽

First Time ◽

Infection Markers

Human bocaviruses (HBoVs) 1–4 belong to the Parvoviridae family, and they infect the respiratory or gastrointestinal tracts in children. We investigated the prevalence of HBoV1–4 DNAs in the blood and stool samples, and of HBoV1–4 IgG and IgM in the plasma samples, of children presenting with acute gastroenteritis (AGE). In addition, we identified HBoV co-infections with the five most frequent gastrointestinal pathogens. A total of 83 paired blood and stool samples were collected from children aged five years or less. Infection markers of HBoV1, 2, or 3 (viral DNA in blood and/or stool and/or antibodies) were detected in 61 out of 83 (73.5%) patients. HBoV1, 2, or 3 DNA as a monoinfection was revealed in 18.1%, 2.4%, and 1.2%, respectively, and 21.7% in total. In 56.1% of the HBoV DNA-positive patients, the presence in stool of another virus—most frequently norovirus or rotavirus—was observed. In conclusion, this study, for the first time, illustrates the prevalence and genetic diversity of HBoVs in Latvian children with gastroenteritis, and shows a widespread distribution of these viruses in the community. HBoV1 and 2 are commonly found as single infectious agents in children with AGE, suggesting that the viruses can be as pathogenic by themselves as other enteric agents are.

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Relative Severity of Common Human Coronaviruses and Influenza in Patients Hospitalized With Acute Respiratory Infection: Results From 8-Year Hospital-Based Surveillance in Quebec, Canada

The Journal of Infectious Diseases ◽

10.1093/infdis/jiaa477 ◽

2020 ◽

Cited By ~ 2

Author(s):

Rodica Gilca ◽

Sara Carazo ◽

Rachid Amini ◽

Hugues Charest ◽

Gaston De Serres

Keyword(s):

Respiratory Infection ◽

Acute Respiratory Infection ◽

Multivariate Analyses ◽

Case Fatality ◽

Viral Etiology ◽

Prospective Data ◽

Estimation Equations ◽

Human Coronaviruses ◽

Few Data

Abstract Background Few data exist concerning the role of common human coronaviruses (HCoVs) in patients hospitalized for acute respiratory infection (ARI) and the severity of these infections compared with influenza. Methods Prospective data on the viral etiology of ARI hospitalizations during the peaks of 8 influenza seasons (from 2011–2012 to 2018–2019) in Quebec, Canada, were used to compare patients with HCoV and those with influenza infections; generalized estimation equations models were used for multivariate analyses. Results We identified 340 HCoV infections, which affected 11.6% of children (n = 136) and 5.2% of adults (n = 204) hospitalized with ARI. The majority of children (75%) with HCoV infections were also coinfected with other respiratory viruses, compared with 24% of the adults (P < .001). No deaths were recorded in children; 5.8% of adults with HCoV monoinfection died, compared with 4.2% of those with influenza monoinfection (P = .23). The risk of pneumonia was nonsignificantly lower in children with HCoV than in those with influenza, but these risks were similarly high in adults. Markers of severity (length of stay, intensive care unit admissions, and case-fatality ratio) were comparable between these infections in multivariate analyses, in both children and adults. Conclusions In children and adults hospitalized with ARI, HCoV infections were less frequent than influenza infections, but were as severe as influenza monoinfections.

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