Human Bocavirus genotypes 1 and 2 detected in younger Amazonian children with acute gastroenteritis or respiratory infections, respectively

Introduction: The Human bocavirus (HBoV) was first identified from the nasopharyngeal aspirate specimen in 2005, which includes four subtypes (HBoV1-4). The HBoV-1 is a major subtype in acute respiratory infections of children, and others (HBoV2-4) present in the stool specimens. The pathogenic role of HBoV2-4 in acute gastroenteritis has not confirmed yet, therefore, it has been considered widely. Case Presentation: In this report, we presented a 2-month-old boy with acute gastroenteritis admitted to the Shahid Beheshti Hospital of Kashan, Iran. The stool sample of the patient was tested for HBoV by polymerase chain reaction (PCR) of the NP-1 gene. The other major gastrointestinal pathogens of Salmonella spp., Shigella spp., Giardia lamblia, and Entamoeba histolytica were confirmed by specialized microbiological procedures and viral pathogen of Rotavirus by the enzyme-linked immunosorbent assay. This case was confirmed by NP-1 plasmid cloned as a positive control. All clinical manifestations were analyzed by a pediatric nurse through hospital admission. Conclusions: This case was found HBoV-positive for the NP-1 gene of 354 bp by PCR. The major signs were diarrhea, fever, dehydration, and abdominal pain. This case was charged after supportive therapies for dehydration. We showed that HBoV could be a gastrointestinal pathogen in pediatric patients and causing diarrhea in young children. However, more studies are needed to confirm.

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Human bocavirus genotypes 1, 2, and 3 circulating in pediatric patients with acute gastroenteritis in Chiang Mai, Thailand, 2012–2018

Journal of Infection and Public Health ◽

10.1016/j.jiph.2020.12.007 ◽

2021 ◽

Vol 14 (2) ◽

pp. 179-186

Author(s):

Nattika Nantachit ◽

Pakawat Kochjan ◽

Pattara Khamrin ◽

Kattareeya Kumthip ◽

Niwat Maneekarn

Keyword(s):

Acute Gastroenteritis ◽

Pediatric Patients ◽

Human Bocavirus ◽

Chiang Mai

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Identification of Human Bocavirus type 4 in a child asymptomatic for respiratory tract infection and acute gastroenteritis – Goiânia, Goiás, Brazil

The Brazilian Journal of Infectious Diseases ◽

10.1016/j.bjid.2017.03.012 ◽

2017 ◽

Vol 21 (4) ◽

pp. 472-476 ◽

Cited By ~ 2

Author(s):

Teresinha Teixeira de Sousa ◽

Tâmera Nunes Vieira Almeida ◽

Fabíola Souza Fiaccadori ◽

Menira Souza ◽

Kareem Rady Badr ◽

...

Keyword(s):

Tract Infection ◽

Respiratory Tract ◽

Respiratory Tract Infection ◽

Acute Gastroenteritis ◽

Human Bocavirus

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Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762012000600015 ◽

2012 ◽

Vol 107 (6) ◽

pp. 800-804 ◽

Cited By ~ 10

Author(s):

Teresinha Teixeira de Sousa ◽

Menira Souza ◽

Fabíola Souza Fiaccadori ◽

Ana Maria Tavares Borges ◽

Paulo Sucasas da Costa ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Human Bocavirus

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Frequency of human bocavirus respiratory infections among at-risk patients in São Paulo, Brazil

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652012000600003 ◽

2012 ◽

Vol 54 (6) ◽

pp. 307-310 ◽

Cited By ~ 3

Author(s):

Elaine Regina Baptista Caccia ◽

Aripuana Sakurada Aranha Watanabe ◽

Emerson Carraro ◽

Elcio Leal ◽

Celso Granato ◽

...

Keyword(s):

At Risk ◽

Respiratory Infections ◽

Tertiary Hospital ◽

Marrow Transplant ◽

Human Bocavirus ◽

Virus Infections ◽

Risk Patients ◽

Adults And Children ◽

Respiratory Virus Infections

BACKGROUND AND OBJECTIVES: Human Bocavirus (HBoV) has been described since 2005 as an etiological agent of respiratory virus infections. From 2001 to 2008 we investigated the etiology of HBoV among adults and children in different groups at risk of presenting complications arising from acute respiratory infection, the investigation was carried out in a tertiary hospital health care system in Brazil. METHODS: HBoV DNA was assayed in 598 respiratory samples from community and hospitalized patients by PCR. RESULTS: Of the 598 tested samples, 2.44% (8/328) of children, including five children with heart disease, and 0.4% (1/270) of adult bone-marrow-transplant were HBoV positive. CONCLUSIONS: These data suggested lower HBoV frequency among different at-risk patients and highlights the need to better understand the real role of HBoV among acute respiratory symptomatic patients.

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Detection of Human Bocavirus Species 2 and 3 in Bivalve Shellfish in Italy

Applied and Environmental Microbiology ◽

10.1128/aem.02754-17 ◽

2018 ◽

Vol 84 (7) ◽

Cited By ~ 2

Author(s):

G. La Rosa ◽

G. Purpari ◽

A. Guercio ◽

S. Di Bella ◽

F. Gucciardi ◽

...

Keyword(s):

Respiratory Infections ◽

Pediatric Population ◽

Human Bocavirus ◽

Spatial Spread ◽

River Waters ◽

Italian Regions ◽

Significant Difference ◽

Tract Infections ◽

Production Areas ◽

Bivalve Shellfish

ABSTRACTHuman bocavirus (HBoV) has been shown to be a common cause of respiratory infections and gastroenteritis in children. Recently, HBoVs have been detected in sewage and river waters in Italy and worldwide. However, studies on their presence in other water environments and in bivalve mollusks are not yet available. In this study, 316 bivalve shellfish samples collected in three Italian regions over a 6-year period (2012 to 2017) were analyzed by nested PCR and sequencing using broad-range primer pairs targeting the capsid proteins VP1 and VP2 of HBoV. The virus was detected in 27 samples (8.5% of the total samples), and a statistically significant difference was found within the three regions. A further 13 samples, collected in geographic and temporal proximity to positive samples, were included in the study to assess the spread of HBoV in shellfish production areas at the time of contamination. Twelve of these additional samples were found to be positive for HBoV. All positive samples in this study were characterized as HBoV species 2 (17 samples; 8 different sequences) or species 3 (22 samples; 4 different sequences). This study reports the occurrence of HBoV in bivalve shellfish and shows evidence of considerable spatial spread of the virus throughout shellfish production areas. Further studies are needed to elucidate both the role of HBoV as an agent of gastroenteritis and the risk for foodborne transmission of this virus.IMPORTANCEHuman bocavirus is recognized as an important cause of acute respiratory tract infections and has recently been considered an etiological agent of gastroenteritis in the pediatric population. Our findings document that HBoVs are detected in bivalve shellfish with a relevant prevalence and suggest that an assessment of the risk for foodborne transmission of these viruses should be undertaken.

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Molecular epidemiology of human bocavirus infection in hospitalized children with acute gastroenteritis in South Africa, 2009‐2015

Journal of Medical Virology ◽

10.1002/jmv.25634 ◽

2019 ◽

Vol 92 (8) ◽

pp. 1124-1132 ◽

Cited By ~ 5

Author(s):

Rembuluwani Netshikweta ◽

Lizyben Chidamba ◽

Sandrama Nadan ◽

Maureen B. Taylor ◽

Nicola A. Page

Keyword(s):

South Africa ◽

Molecular Epidemiology ◽

Acute Gastroenteritis ◽

Human Bocavirus ◽

Hospitalized Children

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Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis

Viruses ◽

10.3390/v10110639 ◽

2018 ◽

Vol 10 (11) ◽

pp. 639 ◽

Cited By ~ 3

Author(s):

Zaiga Nora-Krukle ◽

Anda Vilmane ◽

Man Xu ◽

Santa Rasa ◽

Inga Ziemele ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Human Bocavirus ◽

Plasma Samples ◽

Infectious Agents ◽

Viral Dna ◽

Widespread Distribution ◽

Gastrointestinal Pathogens ◽

Stool Samples ◽

First Time ◽

Infection Markers

Human bocaviruses (HBoVs) 1–4 belong to the Parvoviridae family, and they infect the respiratory or gastrointestinal tracts in children. We investigated the prevalence of HBoV1–4 DNAs in the blood and stool samples, and of HBoV1–4 IgG and IgM in the plasma samples, of children presenting with acute gastroenteritis (AGE). In addition, we identified HBoV co-infections with the five most frequent gastrointestinal pathogens. A total of 83 paired blood and stool samples were collected from children aged five years or less. Infection markers of HBoV1, 2, or 3 (viral DNA in blood and/or stool and/or antibodies) were detected in 61 out of 83 (73.5%) patients. HBoV1, 2, or 3 DNA as a monoinfection was revealed in 18.1%, 2.4%, and 1.2%, respectively, and 21.7% in total. In 56.1% of the HBoV DNA-positive patients, the presence in stool of another virus—most frequently norovirus or rotavirus—was observed. In conclusion, this study, for the first time, illustrates the prevalence and genetic diversity of HBoVs in Latvian children with gastroenteritis, and shows a widespread distribution of these viruses in the community. HBoV1 and 2 are commonly found as single infectious agents in children with AGE, suggesting that the viruses can be as pathogenic by themselves as other enteric agents are.

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