scholarly journals Seagulls of the Berlengas Natural Reserve of Portugal as Carriers of Fecal Escherichia coli Harboring CTX-M and TEM Extended-Spectrum Beta-Lactamases

2008 ◽  
Vol 74 (23) ◽  
pp. 7439-7441 ◽  
Author(s):  
Patricia Poeta ◽  
Hajer Radhouani ◽  
Gilberto Igrejas ◽  
Alexandre Gonçalves ◽  
Carlos Carvalho ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antibiotic Resistance Gene ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Natural Reserve ◽  
Class 1 ◽  
Extended Spectrum Beta Lactamases

ABSTRACT Escherichia coli isolates containing the following extended-spectrum beta-lactamases have been detected in 11 of 57 fecal samples (19.3%) in Berlengas Island seagulls: TEM-52 (eight isolates), CTX-M-1 (one isolate), CTX-M-14a (one isolate), and CTX-M-32 (one isolate). Most of the extended-spectrum beta-lactamase-positive isolates harbored class 1 or class 2 integrons, which included different antibiotic resistance gene cassettes.

scholarly journals Survey of Klebsiella pneumoniae producing extended-spectrum beta-lactamases: prevalence of TEM-3 and first identification of TEM-26 in France.

1996 ◽  
Vol 40 (4) ◽  
pp. 1027-1029 ◽  
Author(s):  
M J Soilleux ◽  
A M Morand ◽  
G J Arlet ◽  
M R Scavizzi ◽  
R Labia
Keyword(s):  
Klebsiella Pneumoniae ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Crude Extracts ◽  
Extended Spectrum Beta Lactamases

Crude extracts from 115 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates were analyzed biochemically. The TEM-3 type was encountered 108 times, SHV types were encountered 7 times, and the TEM-26 type was encountered only once. For the last one, the gene was identified; an adenine was detected at position 925, as in blaTEM-26B not in blaTEM-26.

scholarly journals Molecular characterization of extended-spectrum beta-lactamases in Enterobacteriaceae from patients of two hospitals in Saxony, Germany

2007 ◽  
Vol 56 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Joachim Schmitt ◽  
Enno Jacobs ◽  
Herbert Schmidt
Keyword(s):  
Plasmid Dna ◽  
Klebsiella Oxytoca ◽  
Rflp Analysis ◽  
Beta Lactamase ◽  
M Gene ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Genes Encoding ◽  
Extended Spectrum Beta Lactamases

Between January and September 2003, 39 isolates of the family Enterobacteriaceae with phenotypically positive Vitek 1 extended-spectrum beta-lactamase (ESBL) test results were collected, originating from patients of two hospitals in Saxony, Germany. Plasmid DNA was isolated and screened by PCR for the presence of genes encoding beta-lactamases of SHV, TEM and CTX-M types. To differentiate ESBL and non-ESBL among SHV and TEM genes, detailed analysis of PCR products was performed. Twenty-four strains carried SHV-2, SHV-5 or SHV-12 genes. In a further 11 strains a CTX-M gene was detected. The CTX-M genes could be affiliated to the CTX-M-1 and CTX-M-9 cluster by RFLP analysis. In the case of four Klebsiella oxytoca isolates, hyperproduction of the chromosomal beta-lactamase K1 was inferred, because genes of the above-mentioned types were not detected. The strains contained plasmid DNA between 45 and 160 kb in size. Common plasmid restriction patterns among SHV-5 producers provided evidence of horizontal spread. Twenty strains had a MIC for cefotaxime of ⩽4 mg l−1, 18 strains had the same MIC for ceftazidime, and nine strains had this MIC of >4 mg l−1 for both antibiotics. The ESBL phenotypes often coincided with ciprofloxacin or gentamicin resistance.

scholarly journals The Frequency of Extended-spectrum Beta-lactamases and In-vitro Antibiotic Resistance in Escherichia coli and Klebsiella pneumoniae Strains Isolated from Blood Cultures

ANKEM Dergisi ◽  
2012 ◽  
Vol 26 (4) ◽  
pp. 181-186 ◽  
Author(s):  
Berrin Karaayak Uzun ◽  
Serdar Gungor ◽  
Mujde Serifhan İlgun ◽  
Rahim Ozdemir ◽  
Nurten Baran ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Blood Cultures ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases

scholarly journals The Frequency of Extended-spectrum Beta-lactamases and Antibiotic Resistance Patterns in Escherichia coli Strains Isolated from Blood Cultures

ANKEM Dergisi ◽  
2012 ◽  
Vol 25 (4) ◽  
pp. 250-255
Author(s):  
Derya Saglam ◽  
Suleyman Durmaz ◽  
Huseyin Kilic ◽  
Mustafa Altay Atalay ◽  
Baris Derya Ercal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Blood Cultures ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Resistance Patterns ◽  
Extended Spectrum Beta Lactamases ◽  
Antibiotic Resistance Patterns

scholarly journals Biofilm production in relation to extended spectrum beta-lactamase production and antibiotic resistance among uropathogenic Escherichia coli

2017 ◽  
Vol 5 (1) ◽  
pp. 61-63
Author(s):  
Samip Khatri ◽  
Narayan Dutt Pant ◽  
Sanjeev Neupane ◽  
Suman Bhandari ◽  
Megha Raj Banjara
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Uropathogenic Escherichia Coli ◽  
Beta Lactamase ◽  
Medical Sciences ◽  
Extended Spectrum Beta Lactamase ◽  
Medical College ◽  
Biofilm Production ◽  
Extended Spectrum

Not available. Janaki Medical College Journal of Medical Sciences (2017) Vol. 5(1): 61-63

scholarly journals Relationship between isolation of extended spectrum beta-lactamase-producing Klebsiella pneumoniae and course of hospital-acquired pneumonia

Medicina ◽  
2007 ◽  
Vol 43 (10) ◽  
pp. 778
Author(s):  
Astra Vitkauskienė ◽  
Agnė Giedraitienė ◽  
Vytis Dudzevičius ◽  
Raimundas Sakalauskas
Keyword(s):  
Klebsiella Pneumoniae ◽  
Late Onset ◽  
Lavage Fluid ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Extended Spectrum Beta Lactamases ◽  
Hospital Acquired Pneumonia ◽  
Hospital Acquired

Aim of the study. To evaluate relationship between isolation of extended spectrum beta-lactamaseproducing Klebsiella pneumoniae strains and course of hospital-acquired pneumonia. Materials and methods. K. pneumoniae strains isolated from bronchial secretions or bronchoalveolar lavage fluid samples of patients hospitalized at an intensive care unit of Kaunas University of Medicine Hospital were analyzed. By means of synergistic two-antibiotics disc method, K. pneumoniae strains producing extended spectrum beta-lactamases were selected for further analysis using E-test (AB Biodisk, Solna, Sweden). Hospitalacquired pneumonia was diagnosed based on standard criteria for the diagnosis of pneumonia if signs of pneumonia occurred after 48 hours following admission. Late-onset hospital-acquired pneumonia was considered if these signs of pneumonia occurred on fifth day of hospitalization or later. Results. Total of 45 strains of K. pneumoniae were isolated during the study period; 18 isolated strains produced ESBL. Thirty-two patients investigated have developed hospital-acquired pneumonia, 20 of which were cases of late-onset hospital-acquired pneumonia. Thirteen cases of K. pneumoniae isolation were classified as airway colonization. Extended spectrum beta-lactamase-producing K. pneumoniae strains were more frequently isolated from patients with hospital-acquired pneumonia (88.9%, n=16 and 11.1%, n=2, P<0.05) in comparison with non-producing strains. Extended spectrum beta-lactamase-producing strains were more prevalent in late-onset pneumonia group (93.8%, n=15) than in early-onset group (6.2%, n=1, P<0.001). Conclusions. Extended spectrum beta-lactamase-producing K. pneumoniae strains were more frequently isolated from patients with hospital-acquired pneumonia as compared to colonized patients. Extended spectrum beta-lactamase-producing K. pneumoniae strains were more frequently isolated from patients with late-onset hospital-acquired pneumonia.

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