scholarly journals Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus Strains from Louisiana Retail Meats

2008 ◽  
Vol 75 (1) ◽  
pp. 265-267 ◽  
Author(s):  
Shuaihua Pu ◽  
Feifei Han ◽  
Beilei Ge
Keyword(s):  
Staphylococcus Aureus ◽  
Grocery Stores ◽  
Methicillin Resistant ◽  
Isolation And Characterization ◽  
Retail Meats ◽  
Retail Meat ◽  
Mrsa Strains ◽  
Meat Samples ◽  
Pork Samples

ABSTRACT We investigated the prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in 120 retail meat samples from 30 grocery stores in Baton Rouge, LA. S. aureus strains were recovered from 45.6% of pork samples and 20% of beef samples, whereas MRSA strains were isolated from six meat samples (five pork samples and one beef sample). The MRSA isolates were of two strain types (clones), one harboring Panton-Valentine leucocidin and belonging to pulsed-field gel electrophoresis type USA300 and the other one belonging to USA100.

Genetic characterization of two methicillin-resistant Staphylococcus aureus spa type t127 strains isolated from workers in the dairy production chain in Greece

2021 ◽  
Author(s):  
Theodoros Karampatakis ◽  
Panagiotis Papadopoulos ◽  
Katerina Tsergouli ◽  
Apostolos S. Angelidis ◽  
Daniel Sergelidis ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Characterization ◽  
Food Handler ◽  
Dairy Production ◽  
Whole Genome ◽  
Genome Sequences ◽  
Production Chain ◽  
Methicillin Resistant ◽  
Mrsa Strains

AbstractMethicillin-resistant Staphylococcus aureus (MRSA) constitutes a constant threat for the public health. Aim of the present study was to analyse the whole genome sequences of two MRSA strains belonging to Staphylococcus protein A (spa) type t127 isolated from humans working in two distantly located dairy production farms in Greece.MRSA strains were isolated from the nasal cavity of a food handler in a milk industry in Epirus, northwestern Greece (E-MRSA), and a person working in a cattle farm in Thrace, northeastern Greece (T-MRSA). Whole genome sequences taken using next generation sequencing were analysed for resistance and virulence genes applying various bioinformatic tools.Both isolates were assigned to ST1-IVa-t127 type, and they were transferring genes conferring resistance to tetracycline, β-lactams, and aminoglycosides; T-MRSA was carrying additional genes leading to macrolide, lincosamide and streptogramin B (MLSB) resistance. Both isolates were carrying three plasmid replicon types, rep5, rep7 and rep16, while T-MRSA harboured also rep10 and rep15. E-MRSA carried scn and sak genes which were absent from T-MRSA.In conclusion, the genetic characterization of two unrelated ST1-IVa-t127 MRSA strains isolated from humans in close contact with livestock in Greece can be used as basis for further epidemiological and evolutionary studies.

scholarly journals Characterization of Gentamicin-Susceptible Strains of Methicillin-Resistant Staphylococcus aureus Involved in Nosocomial Spread

1998 ◽  
Vol 36 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Nadine Lemaître ◽  
Wladimir Sougakoff ◽  
Afef Masmoudi ◽  
Marie-Hélène Fievet ◽  
Roland Bismuth ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dna Hybridization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Pulsed Field Gel Electrophoresis ◽  
Cross Infection ◽  
Methicillin Resistant ◽  
Resistance Patterns ◽  
Mrsa Strains ◽  
Selection Of

We report an outbreak of epidemic Staphylococcus aureusstrains characterized by an unusual heterogeneous resistance to methicillin and resistance to tobramycin but susceptibility to gentamicin (gentamicin-susceptible methicillin-resistant S. aureus [GS-MRSA]), contrasting with gentamicin-resistant homogeneous MRSA (GR-MRSA) that have been endemic in our hospital since the 1970s. A total of 97 GS-MRSA strains, which were shown by DNA hybridization to carry the mecA and ant(4′)-Iagenes, were studied. The 40 GS-MRSA strains isolated at the beginning of the outbreak (January 1992 to June 1993) were typed by using resistance patterns, phage typing, serotyping, and pulsed-field gel electrophoresis and were compared with GR-MRSA and methicillin-susceptible S. aureus (MSSA) strains isolated during the same period. Two dominant clones, A::1 and B::3, and one minor clone, C::5, were identified among the 40 GS-MRSA strains, according to pulsotypes (A to C) and their resistance patterns (1, 3, and 5), which were distinguishable from those of GR-MRSA and MSSA strains. A selection of 57 GS-MRSA strains, isolated from 1994 to 1996, were clustered in the same three clones. However, their distribution had changed in comparison with that in the 1992 to 1993 period: clone A::1 remained dominant (47 versus 42.5%), whereas clone B::3 progressively declined (5 versus 35%) and clone C::5, the most susceptible to antibiotics, spread (44 versus 2.5%). Epidemiological investigations revealed that some clones had been introduced via patients transferred from other hospitals and that cross-infection occurred within and between wards. Major changes in the use of antibiotics, especially aminoglycosides, cyclines, and macrolides, likely played a role in the emergence and spread of GS-MRSA strains.

scholarly journals Ability of biofilm production and molecular analysis of spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Mitra Omidi ◽  
Farzaneh Firoozeh ◽  
Mahmood Saffari ◽  
Hossein Sedaghat ◽  
Mohammad Zibaei ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cross Sectional Study ◽  
Clinical Isolates ◽  
Cross Sectional ◽  
Disk Diffusion Test ◽  
Methicillin Resistant ◽  
Isfahan Province ◽  
Biofilm Production ◽  
Mrsa Strains

Abstract Objective This study aimed to evaluate the phenotypic and genotypic characterization of biofilm formation and spa and ica genes among clinical isolates of methicillin-resistant Staphylococcus aureus. Result This cross-sectional study was performed on 146 Staphylococcus aureus isolates from hospitalized patients in Isfahan Province Hospitals. MRSA isolates were confirmed using disk diffusion test with oxacillin disk and amplification of mecA gene by PCR assays. Ability of biofilm production was evaluated targeting the icaA and icaD genes. Of 146 Staphylococcus aureus isolates, 24 (16.4%) carried mecA genes and identified as MRSA strains. Strong ability of biofilm production was seen among 76.02% (111/146) S. aureus isolates and 87.5% (21/24) MRSA strains, respectively. Also, 75.0% (18/24) MRSA isolates carried icaA and icaD was not detected in these strains. Analysis of spa gene showed 70.83% (17/24) MRSA strains were spa positive. From which 14 and 3 strains identified with one band (150, 270, 300, 360, 400 bp) and two bands (150–300 bp), respectively. According to data obtained, the prevalence of MRSA isolates from Isfahan Province Hospitals is relatively high and a remarkable percentage of them show strong power in biofilm production. Also analysis of spa gene showed a fairly large diversity among MRSA strains.

scholarly journals Commercially Distributed Meat as a Potential Vehicle for Community-Acquired Methicillin-Resistant Staphylococcus aureus

2012 ◽  
Vol 78 (8) ◽  
pp. 2797-2802 ◽  
Author(s):  
Kikuyo Ogata ◽  
Hiroshi Narimatsu ◽  
Masahiro Suzuki ◽  
Wataru Higuchi ◽  
Tatsuo Yamamoto ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Susceptibility ◽  
Methicillin Resistant ◽  
Content Type ◽  
Reading Frame ◽  
Type Iv ◽  
Mrsa Infection ◽  
Stool Samples ◽  
Mrsa Strains ◽  
Meat Samples

ABSTRACTThe incidence of community-acquired methicillin-resistantStaphylococcus aureus(CA-MRSA) infection has been increasing; however, the sources of infection remain unclear. Therefore, we investigated the involvement of meat as a possible mediator of CA-MRSA infection. We examined the distribution of MRSA strains in commercially distributed raw meat samples (n= 197) and diarrheal stool samples of outpatients (n= 1,287) that were collected in Oita Prefecture, Japan, between 2003 and 2009 for routine legal inspections. Fourteen MRSA strains were isolated from three meat and 11 stool samples. Among these, seven isolates from three meat and four stool samples exhibited the same epidemiological marker profiles [coagulase type III, staphylococcal enterotoxin C, staphylococcal chromosomal cassettemec(SCCmec) type IV, ST8,spatype 606 (t1767), and toxic shock syndrome toxin-1 (TSST-1) producing type]. Furthermore, of the seven strains, three isolates from two meat samples and one stool sample collected in 2007 exhibited completely identical characteristics with respect to phage open reading frame (ORF) typing, pulsed-field gel electrophoresis, and drug susceptibility profiles. The results suggest that commercially distributed meat could play a role in the prevalence of CA-MRSA in the community.

scholarly journals Molecular Characterization of Community-Associated Methicillin-Resistant Staphylococcus aureus in Iranian Burn Patients

2019 ◽  
Vol 14 (4) ◽  
pp. 284-289 ◽  
Author(s):  
Samira Tajik ◽  
Shahin Najar Peerayeh ◽  
Bita Bakhshi ◽  
Reza Golmohammadi
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Significant Proportion ◽  
Burn Patients ◽  
Burn Wound ◽  
Methicillin Resistant ◽  
Pfge Typing ◽  
Mrsa Strains

Background & Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is reported as one of the important bacterial causes of burn wound infections. This study was carried out to investigate molecular characterization of community-associated MRSA (CA-MRSA) isolated from Iranian burn patients. Methods: A total of 31 isolates of S. aureus were collected fromthe Motahari Burns Hospital (Tehran, Iran) in 2016. All isolates were collected from outpatients and inpatients within 48 hours of admission. The mecA, pvl, tsst-1, hla-α, and psmα genes detecting, SCCmec, agr and PFGE typing were done. Results: A total of 13 (41.9%) isolates were cefoxitin-resistant and mecA-positive, which were considered as MRSA. The SCCmec typing MRSA strains revealed type II in 1 (7.7%), type III in 9 (69.2%), and other types in 3 isolates (23.7%) cases. The agrtyping of all 31 isolates showed that 14 (45.2%), 1 (3.2%), 6 (19.4%), and 10 (32.3%) strains belonged to agr groups 1, 3, 4, and unknown type, respectively. The pvl, tsst-1, hla-α, and psmα genes were positive in 3 (9.7%), 4 (12.9%), 21 (67.7%), and 31 (100%) isolates, respectively. Considering the cut-off values of ≥50%, 3 groups of related isolates (cluster A1, B1, and C1) in PFGE study were observed. Conclusion: The MRSA strains of this study were initially isolated as Community-associated S. aureus (CA-MRSA); however molecular characterization showed that a significant proportion of them had hospital-associated MRSA (HA-MRSA) features. Therefore, it is likely that the HA-MRSA strains are spread among the community.

scholarly journals Phenotypic and Genotypic Characterization of Nosocomial Staphylococcus aureus Isolates from Trauma Patients

1998 ◽  
Vol 36 (2) ◽  
pp. 414-420 ◽  
Author(s):  
T. Na’was ◽  
A. Hawwari ◽  
E. Hendrix ◽  
J. Hebden ◽  
R. Edelman ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Trauma Patients ◽  
Resistant Genotype ◽  
Methicillin Resistant ◽  
University Of Maryland ◽  
Single Clone ◽  
Resistant Strains ◽  
Mrsa Strains ◽  
The University

Staphylococcus aureus is a major cause of nosocomial infections. During the period from March 1992 to March 1994, the patients admitted to the intensive care unit of the University of Maryland Shock Trauma Center were monitored for the development ofS. aureus infections. Among the 776 patients eligible for the study, 60 (7.7%) patients developed 65 incidents of nosocomialS. aureus infections. Of the clinical isolates, 43.1% possessed a polysaccharide type 5 capsule, 44.6% possessed a type 8 capsule, and the remaining 12.3% had capsules that were not typed by the type 5 or type 8 antibodies. Six antibiogram types were noted among the infection-related isolates, with the majority of the types being resistant only to penicillin and ampicillin. It was noted that the majority of cases of pneumonia were caused by relatively susceptible strains, while resistant strains were isolated from patients with bacteremia and other infections. Only 16 (6.3%) of the isolates were found to be methicillin-resistant S. aureus (MRSA). DNA fingerprinting by pulsed-field gel electrophoresis showed 36 different patterns, with characteristic patterns being found for MRSA strains and the strains with different capsular types. Clonal relationships were established, and the origins of the infection-related isolates in each patient were determined. We conclude that (i) nosocomial infection-related isolates from the shock trauma patients did not belong to a single clone, although the predominance of a methicillin-resistant genotype was noted, (ii) most infection-relatedS. aureus isolates were relatively susceptible to antibiotics, but a MRSA strain was endemic, and (iii) for practical purposes, the combination of the results of capsular and antibiogram typing can be used as a useful epidemiological marker.

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