scholarly journals Development of a Method To Produce Hemoglobin in a Bioreactor Culture of Escherichia coli BL21(DE3) Transformed with a Plasmid Containing Plesiomonas shigelloides Heme Transport Genes and Modified Human Hemoglobin Genes

2012 ◽  
Vol 78 (15) ◽  
pp. 5471-5471
Author(s):  
B. J. Z. Smith ◽  
P. Gutierrez ◽  
E. Guerrero ◽  
C. J. Brewer ◽  
D. P. Henderson
Keyword(s):  
Escherichia Coli ◽  
Bioreactor Culture ◽  
Human Hemoglobin ◽  
Plesiomonas Shigelloides ◽  
Escherichia Coli Bl21 ◽  
Heme Transport

scholarly journals Development of a Method To Produce Hemoglobin in a Bioreactor Culture of Escherichia coli BL21(DE3) Transformed with a Plasmid Containing Plesiomonas shigelloides Heme Transport Genes and Modified Human Hemoglobin Genes

2011 ◽  
Vol 77 (18) ◽  
pp. 6703-6705 ◽  
Author(s):  
B. J. Z. Smith ◽  
P. Gutierrez ◽  
E. Guerrero ◽  
C. J. Brewer ◽  
D. P. Henderson
Keyword(s):  
Escherichia Coli ◽  
Dry Weight ◽  
Bioreactor Culture ◽  
Human Hemoglobin ◽  
Plesiomonas Shigelloides ◽  
Content Type ◽  
E Coli ◽  
Escherichia Coli Bl21 ◽  
Heme Transport ◽  
A Cell

ABSTRACTWe describe a method for production of recombinant human hemoglobin byEscherichia coligrown in a bioreactor.E. coliBL21(DE3) transformed with a plasmid containing hemoglobin genes andPlesiomonas shigelloidesheme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.

scholarly journals Enhancement of Recombinant Hemoglobin Production in Escherichia coli BL21(DE3) Containing the Plesiomonas shigelloides Heme Transport System

2008 ◽  
Vol 74 (18) ◽  
pp. 5854-5856 ◽  
Author(s):  
D. M. Villarreal ◽  
C. L. Phillips ◽  
A. M. Kelley ◽  
S. Villarreal ◽  
A. Villaloboz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Transport System ◽  
Human Hemoglobin ◽  
Plesiomonas Shigelloides ◽  
E Coli ◽  
Escherichia Coli Bl21 ◽  
Heme Transport ◽  
Protein Folds ◽  
Hemoglobin Production

ABSTRACT To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.

Enzyme replacement therapy for Morquio A: an active recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in Escherichia coli BL21

2010 ◽  
Vol 37 (11) ◽  
pp. 1193-1201 ◽  
Author(s):  
Alexander Rodríguez ◽  
Ángela J. Espejo ◽  
Alejandra Hernández ◽  
Olga L. Velásquez ◽  
Lina M. Lizaraso ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enzyme Replacement Therapy ◽  
Replacement Therapy ◽  
Escherichia Coli Bl21 ◽  
Enzyme Replacement ◽  
Morquio A

Ethanol effects on the overexpression of heterologous catalase in Escherichia coli BL21 (DE3)

2018 ◽  
Vol 103 (3) ◽  
pp. 1441-1453 ◽  
Author(s):  
Hongchen Zheng ◽  
Zhenxiao Yu ◽  
Wenju Shu ◽  
Xiaoping Fu ◽  
Xingya Zhao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli Bl21

scholarly journals Artificial Biosynthetic Pathway for an Unnatural Terpenoid with an Iridiumcontaining P450

2020 ◽  
Author(s):  
Jing Huang ◽  
Zhennan Liu ◽  
brandon bloomer ◽  
Douglas Clark ◽  
Aindrila Mukhopadhyay ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Synthetic Biology ◽  
Chemical Reactions ◽  
Biosynthetic Pathway ◽  
Metabolic Networks ◽  
Complex Molecules ◽  
Whole Cells ◽  
Porphyrin Complex ◽  
Heme Transport ◽  
Artificial Metalloenzymes

<div>Synthetic biology enables microbial hosts to produce complex molecules that are</div><div>otherwise produced by organisms that are rare or difficult to cultivate, but the structures of these</div><div>molecules are limited to chemical reactions catalyzed by natural enzymes. The integration of</div><div>artificial metalloenzymes (ArMs) that catalyze abiotic reactions into metabolic networks could</div><div>broaden the cache of molecules produced biosynthetically by microorgansms. We report the</div><div>assembly of an ArM containing an iridium-porphyrin complex in the cytoplasm of a terpene</div><div>producing Escherichia coli by a heterologous heme transport machinery, and insertion of this ArM</div><div>into a natural biosynthetic pathway to produce an unnatural terpenoid. This work shows that</div><div>synthetic biology and synthetic chemistry, incorporated together in whole cells, can produce</div><div>molecules previously inaccessible to nature.</div>

Engineered production of pyridoxal 5′-phosphate in Escherichia coli BL21

2021 ◽  
pp. 1-10
Author(s):  
Min He ◽  
Jian Ma ◽  
Qingwei Chen ◽  
Qili Zhang ◽  
Ping Yu
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli Bl21
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