scholarly journals A Lytic Providencia rettgeri Virus of Potential Therapeutic Value Is a Deep-Branching Member of the T5virus Genus

2017 ◽  
Vol 83 (23) ◽  
Author(s):  
Hugo Oliveira ◽  
Graça Pinto ◽  
Hanne Hendrix ◽  
Jean-Paul Noben ◽  
Jan Gawor ◽  
...  
Keyword(s):  
Alternative Methods ◽  
Resistant Bacteria ◽  
Potential Candidate ◽  
Drug Resistant ◽  
Antibiotic Resistant ◽  
Phage Group ◽  
Providencia Rettgeri ◽  
Content Type ◽  
Isolation And Characterization

ABSTRACT Providencia rettgeri is emerging as a new opportunistic pathogen with high antibiotic resistance. The need to find alternative methods to control antibiotic-resistant bacteria and the recent advances in phage therapy motivate the search for new phages able to infect Providencia spp. This study describes the isolation and characterization of an obligatory lytic phage, vB_PreS_PR1 (PR1), with therapeutic potential against drug-resistant P. rettgeri. PR1 is a siphovirus. Its virion DNA size (118,537 bp), transcriptional organization, terminal repeats (10,461 bp), and nicks in the 3′-to-5′ strand are similar to those of phage T5. However, sequence similarities of PR1 to phages of the T5virus genus at the DNA and protein levels are limited, suggesting that it belongs to a new species within the Siphoviridae family. PR1 exhibits the ability to kill P. rettgeri antibiotic-resistant strains, is highly specific to the species, and did not present known genomic markers indicating a temperate lifestyle. The lack of homologies between its proteins and proteins of the only other sequenced Providencia prophage, Redjac, suggests that these two phages evolved separately and may target different host proteins. IMPORTANCE The alarming increase in the number of bacteria resistant to antibiotics has been observed worldwide. This is particularly true for Gram-negative bacteria. For certain of their strains, no effective antibiotics are available. Providencia sp. has been a neglected pathogen but is emerging as a multidrug-resistant bacterium. This has revived interest in bacteriophages as alternative therapeutic agents against this bacterium. We describe the morphological, physiological, and genomic characterization of a novel lytic virus, PR1, which is able to kill drug-resistant P. rettgeri clinical isolates. Genomic and phylogenetic analyses indicate that PR1 is a distant relative of T5virus genus representatives. The lack of known virulence- or temperate lifestyle-associated genes in the genome of PR1 makes this phage a potential candidate for therapeutic use. Analysis of its genome also improves our knowledge of the ecology and diversity of T5-like siphoviruses, providing a new link for evolutionary studies of this phage group.

Isolation and characterization of antibiotic-resistant bacteria from pharmaceutical industrial wastewaters

2015 ◽  
Vol 89 ◽  
pp. 54-61 ◽  
Author(s):  
Leyla Tahrani ◽  
Leila Soufi ◽  
Ines Mehri ◽  
Afef Najjari ◽  
Abdenaceur Hassan ◽  
...  
Keyword(s):  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Industrial Wastewaters ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Antibiotic Resistant Bacteria from Swiftlet Feces in Swiftlet Farm Houses in Sarawak, Malaysia

2013 ◽  
Vol 7 (4) ◽  
pp. 137-143 ◽  
Author(s):  
LEONG SUI SIEN ◽  
SAMUEL LIHAN ◽  
LING TECK YEE ◽  
CHIA HWA CHUAN ◽  
LIM CHAN KOON
Keyword(s):  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of lytic phages TSE1-3 against Enterobacter cloacae

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Komal Ameer Khawaja ◽  
Zaigham Abbas ◽  
Shafiq ur Rehman
Keyword(s):  
Medical Practice ◽  
Enterobacter Cloacae ◽  
Therapeutic Agents ◽  
Alternative Therapies ◽  
Resistant Bacteria ◽  
Gene Products ◽  
Antibiotic Resistant ◽  
Isolation And Characterization ◽  
Alternative Approaches

AbstractThe emergence of antibiotic resistant bacterial pathogens is becoming a major challenge for patient care. The utilization of alternative therapies for infectious diseases other than antibiotics is an urgent need of today medical practice. The utilization of lytic bacteriophages and their gene products as therapeutic agents against antibiotic resistant bacteria is one of the convincing alternative approaches. Here we present the isolation and characterization of three lytic bacteriophages TSE1-3 against

scholarly journals Isolation and Characterization of Antibiotic-Resistant Bacteria from Urinary Tract Infection on Diabetic Mellitus Patients

2020 ◽  
Vol 12 (3) ◽  
pp. 391-396
Author(s):  
M. Kumar ◽  
L. Prince
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Resistant Bacteria ◽  
Klebsiella Aerogenes ◽  
Diabetic Mellitus ◽  
Antibiotic Resistant ◽  
Isolation And Characterization

Diabetes mellitus is a chronic disease-which occurs when the pancreas does not produce enough insulin, or when the body cannot effectively use the insulin it produces. This leads to an increased concentration of glucose in the blood (hyperglycemia). Diabetes mellitus is one of the most challenging health problems in India. The present study was investigated for isolation and characterization of antibiotic-resistant bacteria from urinary tract infection on diabetic mellitus patients. The 118 diabetic urine samples were collected and UTI bacteria using HiChrome UTI Agar was isolated. The diabetic UTI isolates were confirmed as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus saprophyticus, Proteus mirabilis and Klebsiella aerogenes based on morphological and biochemical characteristics. Our study identified that almost all the bacteria were highly sensitive to Cefoperazone/Sulbactam (75/30 mcg), Gentamicin (10 mcg), Meropenem (30 mcg), Piperacillin/Tazobactam (100/10 mcg) and Nitrofurantoin (100 mcg) antibiotics. Interestingly Klebsiella aerogenes alone was found to be more resistant to the entire antibiotics used in this study. The antibiotic resistant Klebsiella aerogenes is one of the biggest treats to human health, antibiotic resistance occurs naturally, but misuse of antibiotics in humans is accelerating the process.

Isolation and Characterization of Antibiotic Resistant Bacteria from the Lane College Soil

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Mariah McCullough ◽  
Aliayah Baker ◽  
Quindarius Payne ◽  
Melanie Van Stry
Keyword(s):  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Isolation And Characterization

scholarly journals Battacin (Octapeptin B5), a New Cyclic Lipopeptide Antibiotic from Paenibacillus tianmuensis Active against Multidrug-Resistant Gram-Negative Bacteria

2011 ◽  
Vol 56 (3) ◽  
pp. 1458-1465 ◽  
Author(s):  
Chao-Dong Qian ◽  
Xue-Chang Wu ◽  
Yi Teng ◽  
Wen-Peng Zhao ◽  
Ou Li ◽  
...  
Keyword(s):  
Polymyxin B ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Resistant Bacteria ◽  
Gram Negative Bacteria ◽  
Drug Resistant ◽  
Antibiotic Resistant ◽  
Gram Negative ◽  
Content Type ◽  
Lipopeptide Antibiotic

ABSTRACTHospital-acquired infections caused by drug-resistant bacteria are a significant challenge to patient safety. Numerous clinical isolates resistant to almost all commercially available antibiotics have emerged. Thus, novel antimicrobial agents, specifically those for multidrug-resistant Gram-negative bacteria, are urgently needed. In the current study, we report the isolation, structure elucidation, and preliminary biological characterization of a new cationic lipopeptide antibiotic, battacin or octapeptin B5, produced from aPaenibacillus tianmuensissoil isolate. Battacin kills bacteriain vitroand has potent activity against Gram-negative bacteria, including multidrug-resistant and extremely drug-resistant clinical isolates. Hospital strains ofEscherichia coliandPseudomonas aeruginosaare the pathogens most sensitive to battacin, with MICs of 2 to 4 μg/ml. The ability of battacin to disrupt the outer membrane of Gram-negative bacteria is comparable to that of polymyxin B, the last-line therapy for infections caused by antibiotic-resistant Gram-negative bacteria. However, the capacity of battacin to permeate bacterial plasma membranes is less extensive than that of polymyxin B. The bactericidal kinetics of battacin correlate with the depolarization of the cell membrane, suggesting that battacin kills bacteria by disrupting the cytoplasmic membrane. Other studies indicate that battacin is less acutely toxic than polymyxin B and has potentin vivobiological activity againstE. coli. Based on the findings of the current study, battacin may be considered a potential therapeutic agent for the treatment of infections caused by antibiotic-resistant Gram-negative bacteria.

scholarly journals Agreement Assessment of Tigecycline Susceptibilities Determined by the Disk Diffusion and Broth Microdilution Methods among Commonly Encountered Resistant Bacterial Isolates: Results from the TigecyclineIn VitroSurveillance in Taiwan (TIST) Study, 2008 to 2010

2011 ◽  
Vol 56 (3) ◽  
pp. 1414-1417 ◽  
Author(s):  
Jien-Wei Liu ◽  
Wen-Chien Ko ◽  
Cheng-Hua Huang ◽  
Chun-Hsing Liao ◽  
Chin-Te Lu ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Total Error ◽  
Error Rates ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Broth Microdilution ◽  
Content Type ◽  
E Coli ◽  
Drug Resistant Bacteria

ABSTRACTThe TigecyclineIn VitroSurveillance in Taiwan (TIST) study, initiated in 2006, is a nationwide surveillance program designed to longitudinally monitor thein vitroactivity of tigecycline against commonly encountered drug-resistant bacteria. This study compared thein vitroactivity of tigecycline against 3,014 isolates of clinically important drug-resistant bacteria using the standard broth microdilution and disk diffusion methods. Species studied included methicillin-resistantStaphylococcus aureus(MRSA;n= 759), vancomycin-resistantEnterococcus faecium(VRE;n= 191), extended-spectrum β-lactamase (ESBL)-producingEscherichia coli(n= 602), ESBL-producingKlebsiella pneumoniae(n= 736), andAcinetobacter baumannii(n= 726) that had been collected from patients treated between 2008 and 2010 at 20 hospitals in Taiwan. MICs and inhibition zone diameters were interpreted according to the currently recommended U.S. Food and Drug Administration (FDA) criteria and the European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria. The MIC90values of tigecycline against MRSA, VRE, ESBL-producingE. coli, ESBL-producingK. pneumoniae, andA. baumanniiwere 0.5, 0.125, 0.5, 2, and 8 μg/ml, respectively. The total error rates between the two methods using the FDA criteria were high: 38.4% for ESBL-producingK. pneumoniaeand 33.8% forA. baumannii. Using the EUCAST criteria, the total error rate was also high (54.6%) forA. baumanniiisolates. The total error rates between these two methods were <5% for MRSA, VRE, and ESBL-producingE. coli. For routine susceptibility testing of ESBL-producingK. pneumoniaeandA. baumanniiagainst tigecycline, the broth microdilution method should be used because of the poor correlation of results between these two methods.

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