scholarly journals Biochemical Characteristics of the Novel Haloalkane Dehalogenase DatA, Isolated from the Plant PathogenAgrobacterium tumefaciensC58

2010 ◽  
Vol 77 (5) ◽  
pp. 1881-1884 ◽  
Author(s):  
Khomaini Hasan ◽  
Andrea Fortova ◽  
Tana Koudelakova ◽  
Radka Chaloupkova ◽  
Mayuko Ishitsuka ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Pathogen ◽  
Biochemical Characterization ◽  
The Novel ◽  
Haloalkane Dehalogenase ◽  
Biochemical Characteristics ◽  
Dimeric Structure ◽  
Racemic Mixtures ◽  
Cooperative Kinetics

ABSTRACTWe report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogenAgrobacterium tumefaciensC58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.

The novel purification and biochemical characterization of a reversible CYP24A1:adrenodoxin complex

2013 ◽  
Vol 136 ◽  
pp. 47-53 ◽  
Author(s):  
Kimberly A. Hartfield ◽  
C. David Stout ◽  
Andrew J. Annalora
Keyword(s):  
Biochemical Characterization ◽  
The Novel

scholarly journals Evidence for an Elongated Dimeric Structure of Heparin-Binding Hemagglutinin from Mycobacterium tuberculosis

2008 ◽  
Vol 190 (13) ◽  
pp. 4749-4753 ◽  
Author(s):  
Carla Esposito ◽  
Maxim V. Pethoukov ◽  
Dmitri I. Svergun ◽  
Alessia Ruggiero ◽  
Carlo Pedone ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Virulence Factor ◽  
Biochemical Characterization ◽  
Coiled Coil ◽  
Heparin Binding ◽  
Truncated Form ◽  
X Ray ◽  
Dimeric Structure ◽  
X Ray Scattering

ABSTRACT Heparin-binding hemagglutinin (HBHA) is a virulence factor of tuberculosis which is responsible for extrapulmonary dissemination of this disease. A thorough biochemical characterization of HBHA has provided experimental evidence of a coiled-coil nature of HBHA. These data, together with the low-resolution structures of a full-length form and a truncated form of HBHA obtained by small-angle X-ray scattering, have unambiguously indicated that HBHA has a dimeric structure with an elongated shape.

scholarly journals Biochemical Characterization of the Flagellar Rod Components of Rhodobacter sphaeroides: Properties and Interactions

2015 ◽  
Vol 198 (3) ◽  
pp. 544-552 ◽  
Author(s):  
Manuel Osorio-Valeriano ◽  
Javier de la Mora ◽  
Laura Camarena ◽  
Georges Dreyfus
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Basal Body ◽  
Biochemical Characterization ◽  
High Molecular Mass ◽  
Content Type ◽  
Biochemical Characteristics ◽  
Outer Membranes ◽  
Architectural Features ◽  
Rotary Motor

ABSTRACTThe flagellar basal body is a rotary motor that spans the cytoplasmic and outer membranes. The rod is a drive shaft that transmits torque generated by the motor through the hook to the filament that propels the bacterial cell. The assembly and structure of the rod are poorly understood. In a first attempt to characterize this structure in the alphaproteobacteriumRhodobacter sphaeroides, we overexpressed and purified FliE and the four related rod proteins (FlgB, FlgC, FlgF, and FlgG), and we analyzed their ability to form homo-oligomers. We found that highly purified preparations of these proteins formed high-molecular-mass oligomers that tended to dissociate in the presence of NaCl. As predicted byin silicomodeling, the four rod proteins share architectural features. Using affinity blotting, we detected the heteromeric interactions between these proteins. In addition, we observed that deletion of the N- and C-terminal regions of FlgF and FlgG severely affected heteromeric but not homomeric interactions. On the basis of our findings, we propose a model of rod assembly in this bacterium.IMPORTANCEDespite the considerable amount of research on the structure and assembly of other flagellar axial structures that has been conducted, the rod has been barely studied. An analysis of the biochemical characteristics of the flagellar rod components of the Fla1 system ofR. sphaeroidesis presented in this work. We also analyze the interactions of these proteins with each other and with their neighbors, and we propose a model for the order in which they are assembled.

scholarly journals Biochemical characterization of the novel endo -β-mannanase At Man5-2 from Arabidopsis thaliana

Plant Science ◽  
2015 ◽  
Vol 241 ◽  
pp. 151-163 ◽  
Author(s):  
Yang Wang ◽  
Shoaib Azhar ◽  
Rosaria Gandini ◽  
Christina Divne ◽  
Ines Ezcurra ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Biochemical Characterization ◽  
The Novel

scholarly journals Physical and Biochemical Characterization of Sweet Corn Ears of Four Varieties Grown in Senegal

2017 ◽  
Vol 13 (33) ◽  
pp. 232
Author(s):  
Nafissatou Diop Ndiaye ◽  
Mady Cisse ◽  
Fatou Diop Mbacke ◽  
Abou Diop ◽  
Saliou Ndiaye ◽  
...  
Keyword(s):  
Reducing Sugars ◽  
Biochemical Characterization ◽  
Sweet Corn ◽  
Fertilization Rate ◽  
Human Consumption ◽  
Corn Production ◽  
Biochemical Characteristics ◽  
Underutilized Crop

Sweet corn is an underutilized crop for human consumption in Senegal. In this study, physical and biochemical characterization were performed on four sweet corn ear varieties grown at three different fertilization rate of 15N-15-P-15K. Treatment F1 was 40 tons/Ha horse-dung + 200 kg/Ha of 15N-15P-15K, treatment F2 was 30 tons/Ha horse-dung + 150 kg/Ha of 15N-15P-15K) and treatment F3 was 50 tons/Ha horse-dung + 250 kg/Ha of 15N-15P-15K. Results showed that Yosemite cultivar gave the highest number of kernels/ear (672.3), followed respectively by Legacy (642), Excellis Garrison (585.6) and Infinity (573). Furthermore, treatments using fertilizer 3 (F3: 50 tons/Ha horse-dung + 250 kg/Ha of 15N-15P-15K) led to the highest number of kernels/ear in all cultivars used. Interestingly, Legacy cultivar gave more reducing sugars (5.1 ± 0.8 g.100g -1 ) with treatment F1; treatment F3 provided less reducing sugars (2.1 ± 0.6 g.100g -1 ) and more starch (14.4 ± 0.5 g.100g -1 ). Overall, physical and biochemical characteristics were influenced to at least some extent by fertilization treatments related to the cultivar used. Yosemite and Legacy cultivars would be more suitable for canned sweet corn production in the conditions studied. Sangalkam seems to be more suitable for sweet corn production.

scholarly journals Biochemical characterization of avirulent exoC mutants of Agrobacterium tumefaciens.

1990 ◽  
Vol 172 (3) ◽  
pp. 1640-1646 ◽  
Author(s):  
A D Uttaro ◽  
G A Cangelosi ◽  
R A Geremia ◽  
E W Nester ◽  
R A Ugalde
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Biochemical Characterization

Biochemical characterization of the novel α-1, 3-galactosyltransferase WclR from Escherichia coli O3

2016 ◽  
Vol 430 ◽  
pp. 36-43 ◽  
Author(s):  
Chao Chen ◽  
Bin Liu ◽  
Yongchang Xu ◽  
Natalia Utkina ◽  
Dawei Zhou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biochemical Characterization ◽  
The Novel

Structural and biochemical characterization of the novel serpin Iripin-5 from Ixodes ricinus

2021 ◽  
Vol 77 (9) ◽  
pp. 1183-1196 ◽  
Author(s):  
Barbora Kascakova ◽  
Jan Kotal ◽  
Larissa Almeida Martins ◽  
Zuzana Berankova ◽  
Helena Langhansova ◽  
...  
Keyword(s):  
Ixodes Ricinus ◽  
Biochemical Characterization ◽  
Neutrophil Migration ◽  
Stable State ◽  
Host Immunity ◽  
The Novel ◽  
Defence Mechanisms ◽  
Reactive Centre Loop ◽  
Main Substrate

Iripin-5 is the main Ixodes ricinus salivary serpin, which acts as a modulator of host defence mechanisms by impairing neutrophil migration, suppressing nitric oxide production by macrophages and altering complement functions. Iripin-5 influences host immunity and shows high expression in the salivary glands. Here, the crystal structure of Iripin-5 in the most thermodynamically stable state of serpins is described. In the reactive-centre loop, the main substrate-recognition site of Iripin-5 is likely to be represented by Arg342, which implies the targeting of trypsin-like proteases. Furthermore, a computational structural analysis of selected Iripin-5–protease complexes together with interface analysis revealed the most probable residues of Iripin-5 involved in complex formation.

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