scholarly journals Direct fermentation of potato starch to ethanol by cocultures of Aspergillus niger and Saccharomyces cerevisiae.

1986 ◽  
Vol 52 (5) ◽  
pp. 1055-1059 ◽  
Author(s):  
M M Abouzied ◽  
C A Reddy
2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Jorg C de Ruijter ◽  
Kiyohiko Igarashi ◽  
Merja Penttilä

ABSTRACT Processed lignocellulosic biomass is a source of mixed sugars that can be used for microbial fermentation into fuels or higher value products, like chemicals. Previously, the yeast Saccharomyces cerevisiae was engineered to utilize its cellodextrins through the heterologous expression of sugar transporters together with an intracellular expressed β-glucosidase. In this study, we screened a selection of eight (putative) cellodextrin transporters from different yeast and fungal hosts in order to extend the catalogue of available cellobiose transporters for cellobiose fermentation in S. cerevisiae. We confirmed that several in silico predicted cellodextrin transporters from Aspergillus niger were capable of transporting cellobiose with low affinity. In addition, we found a novel cellobiose transporter from the yeast Lipomyces starkeyi, encoded by the gene Ls120451. This transporter allowed efficient growth on cellobiose, while it also grew on glucose and lactose, but not cellotriose nor cellotetraose. We characterized the transporter more in-depth together with the transporter CdtG from Penicillium oxalicum. CdtG showed to be slightly more efficient in cellobiose consumption than Ls120451 at concentrations below 1.0 g/L. Ls120451 was more efficient in cellobiose consumption at higher concentrations and strains expressing this transporter grew slightly slower, but produced up to 30% more ethanol than CdtG.


Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3689 ◽  
Author(s):  
Federica Moccia ◽  
Adriana C. Flores-Gallegos ◽  
Mónica L. Chávez-González ◽  
Leonardo Sepúlveda ◽  
Stefania Marzorati ◽  
...  

Fermentation in solid state culture (SSC) has been the focus of increasing interest because of its potential for industrial applications. In previous studies SSC of pomegranate wastes by Aspergillus niger has been extensively developed and optimized for the recovery of ellagic acid (EA), a high value bioactive. In this study we comparatively investigated the SSC of powdered pomegranate husks by A. niger and Saccharomyces cerevisiae and evaluated the recovery yields of EA by an ultrasound and microwave-assisted 7:3 water/ethanol extraction. Surprisingly enough, the yields obtained by S. cerevisiae fermentation (4% w/w) were found 5-fold higher than those of the A. niger fermented material, with a 10-fold increase with respect to the unfermented material. The EA origin was traced by HPLC analysis that showed a significant decrease in the levels of punicalagin isomers and granatin B and formation of punicalin following fermentation. Other extraction conditions that could warrant a complete solubilization of EA were evaluated. Using a 1:100 solid to solvent ratio and DMSO as the solvent, EA was obtained in 4% yields from S. cerevisiae fermented husks at a high purity degree. Hydrolytic treatment of S. cerevisiae fermented pomegranate husks afforded a material freed of the polysaccharides components that gave recovery yields of EA up to 12% w/w.


2011 ◽  
Vol 332-334 ◽  
pp. 164-167
Author(s):  
Zheng Li ◽  
Zhen Feng Dong ◽  
Jian Fei Zhang ◽  
Ji Xian Gong ◽  
Fan Jie Meng

In order to determined the antibacterial property of Apocynum venetum. Three kinds of extractants (distilled water, 75% ethanol and absolute ethanol) were used to extract the leaf, stick and skin of Apocynum venetum, respectively. Flask and plate methods were used to evaluate the antibacterial property of these extracts on four kinds of classical microbiologies, Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae and Aspergillus niger, respectively. The results showed that the antibacterial rate of extract by 75 % ethanol was better than extracts by water or absolute ethanol on Escherichia coli and skin contained more antibacterial substance (on Escherichia coli) than others. The antibacterial rate of extract by distill water was better than extracts by 75 % ethanol or absolute ethanol on Staphylococcus aureus and stick contained more antibacterial substance (on Staphylococcus aureus) than others. Some of extracts had very weak antibacterial rate on Saccharomyces cerevisiae and all extracts had not antibacterial effect on Aspergillus niger.


Author(s):  
Sabarta Sembiring ◽  
Pratiwi Trisunuwati ◽  
Osfar Sjofjan ◽  
Irfan Djunaidi

This study evaluated the nutritional and tannin contents of cooked Kepok banana corm, fermented with Saccharomyces cerevisiae and Aspergillus niger. Corm meal was pretreated by steam for 1 hour at 102 0C before fermenting. The pretreated corms were inoculated with 10% (w/v) inoculum dose with additional nutrient mono-culture or co-culture. A completely randomized design with a 3 x 3 factorial arrangement was used to investigate two main factors: microbial strains and incubation time, with three subfactors each, resulting in nine treatment combinations and three replications. The microbial strain and incubation time significantly (P>0.01) affected the nutrient content of fermented Kepok banana corms. The highest dry matter content (91.84%) was achieved by incubation with S. cerevisiae for 48 h, while 89.61% organic matter was obtained by fermentation with both S. cerevisiae + A. niger for 48 h. The highest crude protein content was 5.81%, which resulted from treatment with S. cerevisiae for 96 h, increasing the crude protein by 61% compared to the control (3.58% crude protein). Fermentation by the microbe consortium (S. cerevisiae + A. niger) for 72 h, produced maximum starch (35.54 g/100 g) and energy (3511 Kcal/kg) values. Thus, the fermented products are a potential source of energy, to be used as feed ingredients.


1990 ◽  
Vol 18 (6) ◽  
pp. 531-536 ◽  
Author(s):  
H. Whittington ◽  
S. Kerry-Williams ◽  
K. Bidgood ◽  
N. Dodsworth ◽  
J. Peberdy ◽  
...  

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