Effect of gene amplification on mercuric ion reduction activity of Escherichia coli.

Reconstruction and application of genome-scale metabolic models (GEMs) have facilitated metabolic engineering by providing a platform on which systematic computational analysis of metabolic networks can be performed.

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Escherichia coli as Biosensor for Chromogenic Mercuric Ion Detection

Biosensors '94 ◽

10.1016/b978-1-85617-242-4.50279-2 ◽

1994 ◽

pp. 335

Author(s):

Joachim Klein ◽

Josef Altenbuchner ◽

Ralf Mattes

Keyword(s):

Escherichia Coli ◽

Ion Detection ◽

Mercuric Ion

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Activation by Gene Amplification ofpitB, Encoding a Third Phosphate Transporter ofEscherichia coli K-12

Journal of Bacteriology ◽

10.1128/jb.183.15.4659-4663.2001 ◽

2001 ◽

Vol 183 (15) ◽

pp. 4659-4663 ◽

Cited By ~ 20

Author(s):

Sally M. Hoffer ◽

Paul Schoondermark ◽

Hendrik W. van Veen ◽

Jan Tommassen

Keyword(s):

Escherichia Coli ◽

Gene Amplification ◽

Inorganic Phosphate ◽

Double Mutant ◽

Phosphate Transporter ◽

Ofescherichia Coli ◽

Two Systems ◽

K 12

ABSTRACT Two systems for the uptake of inorganic phosphate (Pi) in Escherichia coli, PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on Pi was isolated. We demonstrate that the expression of a new Pi transporter, PitB, is activated in this strain by a gene amplification event.

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Analysis of Spontaneous Deletions and Gene Amplification in the lac Region of Escherichia coli

Cold Spring Harbor Symposia on Quantitative Biology ◽

10.1101/sqb.1983.047.01.096 ◽

1983 ◽

Vol 47 (0) ◽

pp. 841-850 ◽

Cited By ~ 11

Author(s):

A.M. Albertini ◽

M. Hofer ◽

M.P. Calos ◽

T.D. Tlsty ◽

J.H. Miller

Keyword(s):

Escherichia Coli ◽

Gene Amplification

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Resistance to piperacillin/tazobactam in Escherichia coli resulting from extensive IS26-associated gene amplification of blaTEM-1

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkz349 ◽

2019 ◽

Vol 74 (11) ◽

pp. 3179-3183 ◽

Cited By ~ 8

Author(s):

Katrine Hartung Hansen ◽

Minna Rud Andreasen ◽

Martin Schou Pedersen ◽

Henrik Westh ◽

Lotte Jelsbak ◽

...

Keyword(s):

Escherichia Coli ◽

Gene Amplification ◽

Copy Number ◽

Chromosomal Location ◽

Sequencing Data ◽

Pcr Screening ◽

E Coli ◽

Oxford Nanopore ◽

Additional Strain ◽

Resistance To Penicillin

Abstract Background bla TEM-1 encodes a narrow-spectrum β-lactamase that is inhibited by β-lactamase inhibitors and commonly present in Escherichia coli. Hyperproduction of blaTEM-1 may cause resistance to penicillin/β-lactamase inhibitor (P/BLI) combinations. Objectives To characterize EC78, an E. coli bloodstream isolate, resistant to P/BLI combinations, which contains extensive amplification of blaTEM-1 within the chromosome. Methods EC78 was sequenced using Illumina and Oxford Nanopore Technology (ONT) methodology. Configuration of blaTEM-1 amplification was probed using PCR. Expression of blaTEM-1 mRNA was determined using quantitative PCR and β-lactamase activity was determined spectrophotometrically in a nitrocefin conversion assay. Growth rate was assessed to determine fitness and stability of the gene amplification was assessed by passage in the absence of antibiotics. Results Illumina sequencing of EC78 identified blaTEM-1B as the only acquired β-lactamase preceded by the WT P3 promoter and present at a copy number of 182.6 with blaTEM-1B bracketed by IS26 elements. The chromosomal location of the IS26-blaTEM-1B amplification was confirmed by ONT sequencing. Hyperproduction of blaTEM-1 was confirmed by increased transcription of blaTEM-1 and β-lactamase activity and associated with a significant fitness cost; however, the array was maintained at a relatively high copy number for 150 generations. PCR screening for blaTEM amplification of isolates resistant to P/BLI combinations identified an additional strain containing an IS26-associated amplification of a blaTEM gene. Conclusions IS26-associated amplification of blaTEM can cause resistance to P/BLI combinations. This adaptive mechanism of resistance may be overlooked if simple methods of genotypic prediction (e.g. gene presence/absence) are used to predict antimicrobial susceptibility from sequencing data.

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