scholarly journals Sulfonates as Terminal Electron Acceptors for Growth of Sulfite-Reducing Bacteria (Desulfitobacterium spp.) and Sulfate-Reducing Bacteria: Effects of Inhibitors of Sulfidogenesis

1999 ◽  
Vol 65 (10) ◽  
pp. 4611-4617 ◽  
Author(s):  
Thomas J. Lie ◽  
Walter Godchaux ◽  
Edward R. Leadbetter
Keyword(s):  
Sulfate Reduction ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Electron Acceptors ◽  
Sulfate Reducing ◽  
Desulfitobacterium Hafniense ◽  
Reducing Bacteria

ABSTRACT This study demonstrates the ability ofDesulfitobacterium spp. to utilize aliphatic sulfonates as terminal electron acceptors (TEA) for growth. Isethionate (2-hydroxyethanesulfonate) reduction by Desulfitobacterium hafniense resulted in acetate as well as sulfide accumulation in accordance with the expectation that the carbon portion of isethionate was oxidized to acetate and the sulfur was reduced to sulfide. The presence of a polypeptide, approximately 97 kDa, was evident in isethionate-grown cells of Desulfitobacterium hafniense, Desulfitobacterium sp. strain PCE 1, and the two sulfate-reducing bacteria (SRB)—Desulfovibrio desulfuricans IC1 (T. J. Lie, J. R. Leadbetter, and E. R. Leadbetter, Geomicrobiol. J. 15:135–149, 1998) andDesulfomicrobium norvegicum; this polypeptide was not detected when these bacteria were grown on TEA other than isethionate, suggesting involvement in its metabolism. The sulfate analogs molybdate and tungstate, effective in inhibiting sulfate reduction by SRB, were examined for their effects on sulfonate reduction. Molybdate effectively inhibited sulfonate reduction by strain IC1 and selectively inhibited isethionate (but not cysteate) reduction byDesulfitobacterium dehalogenans andDesulfitobacterium sp. strain PCE 1.Desulfitobacterium hafniense, however, grew with both isethionate and cysteate in the presence of molybdate. In contrast, tungstate only partially inhibited sulfonate reduction by both SRB and Desulfitobacterium spp. Similarly, another inhibitor of sulfate reduction, 1,8-dihydroxyanthraquinone, effectively inhibited sulfate reduction by SRB but only partially inhibited sulfonate reduction by both SRB and Desulfitobacterium hafniense.

scholarly journals Reduction of perchlorate ions by the sulfate-reducing bacteria Desulfotomaculum sp. and Desulfovibrio desulfuricans

2020 ◽  
Vol 11 (2) ◽  
pp. 278-282
Author(s):  
N. S. Verkholiak ◽  
T. B. Peretyatko ◽  
A. A. Halushka
Keyword(s):  
Sewage Treatment ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Inhibitory Effect ◽  
Electron Acceptors ◽  
Wood Chips ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Perchlorate Ion ◽  
Reducing Bacteria

The usage of microorganisms to clean the environment from xenobiotics, in particular chlorine-containing ones, is a promising method of detoxifying the contaminated environment. Sulfate-reducing bacteria Desulfovibrio desulfuricans Ya-11, isolated from Yavoriv Lake, and Desulfotomaculum AR1, isolated from the Lviv sewage treatment system, are able to grow under conditions of environmental contamination by aromatic compounds and chlorine-containing substances. Due to their high redox potential, chlorate and perchlorate ions can be ideal electron acceptors for the metabolism of microorganisms. To test the growth of the tested microorganisms under the influence of perchlorate ions, bacteria were cultured in modified Postgate C medium with ClO4–. Biomass was determined turbidimetrically, the content of sulfate ions and hydrogen sulfide – photoelectrocolorimetrically, the content of perchlorate ions – permanganatometrically. The study of the ability of sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 to grow in a medium with perchlorate ions as electron acceptors showed the inhibitory effect of ClO4– on sulfate ion reduction by bacteria. Bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to grow in environments with aromatic hydrocarbons, in particular toluene. The possibility of the growth of sulfate-reducing bacteria in the presence of toluene as an electron donor and perchlorate ions as an electron acceptor was investigated. The efficiency of perchlorate ion utilization by sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was about 90 %. The effect of molybdenum on the reduction of perchlorate ions by Desulfotomaculum AR1 is shown in the paper. Immobilization of bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was carried out in 3% agar and on wood chips. The ability of bacteria, immobilized on these media, to purify the aqueous medium from perchlorate ions was investigated. Reduction of perchlorate ions is more efficiently performed by cells of Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria immobilized in agar than on wood chips. Sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to use perchlorate ions as electron acceptors, purifying the polluted aquatic environment from these pollutants.

scholarly journals Dissimilatory sulfate reduction in bacteria Desulfovibrio desulfuricans ІМV К-6 upon influence of Uragan and Raundup herbicides

2015 ◽  
Vol 6 (1) ◽  
pp. 40-44
Author(s):  
G. І. Zvir ◽  
O. М. Moroz ◽  
S. O. Hnatush
Keyword(s):  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Colorimetric Method ◽  
Reduction Process ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Objects of the study were sulfate-reducing bacteria Desulfovibrio desulfuricans ІМV К-6, isolated from Yavorivske lakе. This strain is kept in the collection of microorganisms at the Department of Microbiology of Ivan Franko National University. Bacteria were grown in the Kravtsov-Sorokin’s liquid medium with the following composition (g/l): Na2SO4 × 10H2O – 0.5, NaH2PO4 – 0.3, K2HPO4 – 0.5, (NH4)2SO4 – 0.2, MgSO4 × 7H2O – 0.1, C3H5O3Na – 2.0. The bacteria were grown for 10 days at 30 °C under anaerobic conditions. In order to study the sensitivity of the sulfate reducing bacteria to action of Uragan and Raundup herbicides, the cells of D. desulfuricans ІМV К-6 were grown at the concentrations of herbicides as follows: 0,28 mМ, 2,8 mМ (concentration recommended for use) and 5,6 mM. Biomass was determined by photometric method. Concentration of hydrogen sulfide in the culture medium was determined by photo-colorimetric method. Concentration of sulfate-ions in the medium was determined by turbidimetric method. Capacity of sulfate reducing bacteria D. desulfuricans ІМV K-6 to grow, reducing sulfates to hydrogen sulfide upon influence of Uragan and Raundup herbicides was studied. Accumulation of bacterial biomass in the control and upon influence of herbicides was the highest on the fourth-sixth day of cultivation, and after that the stationary growth phase began. It was shown that sulfate reducing bacteria upon influence of herbicides grew more intensively compared with the control. It was discovered that the level of biomass changed depending on the increasing concentration of Uragan or Raundup herbicides in the medium. Sulfate reducing bacteria D. desulfuricans ІМV K-6 could reduce sulfates to hydrogen sulfide in the presence of sulfates and organic compounds in the medium (dissimilatory sulfate reduction). Stimulatory influence of Uragan and Raundup on the dissimilatory sulfate reduction process of D. desulfuricans ІМВ К-6 has been discovered. The formation of hydrogen sulfide correlates with the usage of sulfatе ions. The capacity of sulfate reducing bacteria D. desulfuricans ІМV K-6 to grow, reducing sulfate ions to hydrogen sulfide upon influence of Uragan and Raundup may be caused by presence of inert components (sulfates) in these herbicides that can be used by microorganisms as electron acceptors during sulfate respiration. 

The influence of potassium dichromate on dissimilatory reduction of sulfate and nitrate ions by bacteria Desulfovibrio sp.

2017 ◽  
Vol 28 (1-2) ◽  
pp. 84-95
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
Ch. I. Bohoslavets ◽  
T. M. Hrytsun’ ◽  
B. M. Borsukevych
Keyword(s):  
Electron Acceptor ◽  
Potassium Dichromate ◽  
Anaerobic Respiration ◽  
Sulfate Reducing Bacteria ◽  
Negative Influence ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Nitrate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate reducing bacteria, capable to reductive transformation of different nature pollutants, used in biotechnologies of purification of sewage, contaminated by carbon, sulfur, nitrogen and metal compounds. H2S formed by them sediment metals to form of insoluble sulfides. Number of metals can be used by these microorganisms as electron acceptors during anaerobic respiration. Because under the influence of metal compounds observed slowing of bacteria metabolism, selection isolated from technologically modified ecotops resistant to pollutions strains is important task to create a new biotechnologies of purification. That’s why the purpose of this work was to study the influence of potassium dichromate, present in medium, on reduction of sulfate and nitrate ions by sulfate reducing bacteria Desulfovibrio desulfuricans IMV K-6, Desulfovibrio sp. Yav-6 and Desulfovibrio sp. Yav-8, isolated from Yavorivske Lake, to estimate the efficiency of possible usage of these bacteria in technologies of complex purification of environment from dangerous pollutants. Bacteria were cultivated in modified Kravtsov-Sorokin medium without SO42- and FeCl2×4H2O for 10 days. To study the influence of K2Cr2O7 on usage by bacteria SO42- or NO3- cells were seeded to media with Na2SO4×10H2O or NaNO3 and K2Cr2O7 at concentrations of 1.74 mM for total content of electron acceptors in medium 3.47 mM (concentration of SO42- in medium of standard composition). Cells were also seeded to media with 3.47 mM Na2SO4×10H2O, NaNO3 or K2Cr2O7 to investigate their growth in media with SO42-, NO3- or Cr2O72- as sole electron acceptor (control). Biomass was determined by turbidymetric method, content of sulfate, nitrate, dichromate, chromium (III) ions, hydrogen sulfide or ammonia ions in cultural liquid – by spectrophotometric method. It was found that K2Cr2O7 inhibits growth (2.2 and 1.3 times) and level of reduction by bacteria sulfate or nitrate ions (4.2 and 3.0 times, respectively) at simultaneous addition into cultivation medium of 1.74 mM SO42- or NO3- and 1.74 mM Cr2O72-, compared with growth and level of reduction of sulfate or nitrate ions in medium only with SO42- or NO3- as sole electron acceptor. Revealed that during cultivation of bacteria in presence of equimolar amount of SO42- or NO3- and Cr2O72-, last used by bacteria faster, content of Cr3+ during whole period of bacteria cultivation exceeded content H2S or NH4+. K2Cr2O7 in medium has most negative influence on dissimilatory reduction by bacteria SO42- than NO3-, since level of nitrate ions reduction by cells in medium with NO3- and Cr2O72- was a half times higher than level of sulfate ions reduction by it in medium with SO42- and Cr2O72-. The ability of bacteria Desulfovibrio sp. to priority reduction of Cr2O72- and after their exhaustion − NO3- and SO42- in the processes of anaerobic respiration can be used in technologies of complex purification of environment from toxic compounds.

Microbial ecology of sulfate-reducing bacteria in wastewater biofilms analyzed by microelectrodes and fish (fluorescent in situ hybridization) technique

1999 ◽  
Vol 39 (7) ◽  
pp. 41-47 ◽  
Author(s):  
Satoshi Okabe ◽  
Hisashi Satoh ◽  
Tsukasa Itoh ◽  
Yoshimasa Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Hybridization Technique ◽  
Concentration Profiles ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Culture Dependent

The vertical distribution of sulfate-reducing bacteria (SRB) in microaerophilic wastewater biofilms grown on fully submerged rotating disk reactors (RDR) was determined by the conventional culture-dependent MPN method and in situ hybridization of fluorescently-labelled 16S rRNA-targeted oligonucleotide probes for SRB in parallel. Chemical concentration profiles within the biofilm were also measured using microelectrodes for O2, S2-, NO3- and pH. In situ hybridization revealed that the SRB probe-stained cells were distributed throughout the biofilm even in the oxic surface zone in all states from single scattered cells to clustered cells. The higher fluorescence intensity and abundance of SRB probe-stained cells were found in the middle part of the biofilm. This result corresponded well with O2 and H2S concentration profiles measured by microelectrodes, showing sulfate reduction was restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. Results of the MPN and potential sulfate reducing activity (culture-dependent approaches) indicated a similar distribution of cultivable SRB in the biofilm. The majority of the general SRB probe-stained cells were hybridized with SRB 660 probe, suggesting that one important member of the SRB in the wastewater biofilm could be the genus Desulfobulbus. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from O2 and NO3- respiration zones. Anaerobic H2S oxidation with NO3- was also induced by addition of nitrate to the medium.

Experimental Investigation on the Active Range of Sulfate-Reducing Bacteria for Geological Disposal

1994 ◽  
Vol 353 ◽  
Author(s):  
S. Fukunaga ◽  
H. Yoshikawa ◽  
K. Fujiki ◽  
H. Asano
Keyword(s):  
Experimental Investigation ◽  
Environmental Conditions ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Geological Disposal ◽  
Sulfate Reducing ◽  
Buffer Material ◽  
Ph Range ◽  
High Level ◽  
Reducing Bacteria

AbstractThe active range ofDesulfovibrio desulfuricans. a species of sulfate-reducing bacteria, was examined in terms of pH and Eh using a fermenter at controlled pH and Eh. Such research is important because sulfate-reducing bacteria (SRB) are thought to exist underground at depths equal to those of supposed repositories for high-level radioactive wastes and to be capable of inducing corrosion of the metals used in containment vessels.SRB activity was estimated at 35°C, with lactate as an electron donor, at a pH range from 7 to 11 and Eh range from 0 to -380 mV. Activity increased as pH approached neutral and Eh declined. The upper pH limit for activity was between 9.9 and 10.3, at Eh of -360 to -384 mV. The upper Eh limit for activity was between -68 and -3 mV, at pH 7.1. These results show that SRB can be made active at higher pH by decreasing Eh, and that the higher pH levels of 8 to 10 produced by use of the buffer material bentonite does not suppress SRB completely.A chart was obtained showing the active range ofDesulfovibrio desulfuricansin terms of pH and Eh. Such charts can be used to estimate the viability of SRB and other microorganisms when the environmental conditions of a repository are specified.

scholarly journals Usage of metals as the terminal electron acceptors by the sulfate-reducing bacteria

2009 ◽  
Vol 3 (3) ◽  
pp. 141-158 ◽  
Author(s):  
T. B. Peretyatko ◽  
◽  
A. A. Halushka ◽  
S. P. Gudz ◽  
◽  
...  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Electron Acceptors ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Interactions between filamentous sulfur bacteria, sulfate reducing bacteria and poly-P accumulating bacteria in anaerobic-oxic activated sludge from a municipal plant

1998 ◽  
Vol 37 (4-5) ◽  
pp. 599-603 ◽  
Author(s):  
Ryoko Yamamoto-Ikemoto ◽  
Saburo Matsui ◽  
Tomoaki Komori ◽  
Edja. Kofi. Bosque-Hamilton
Keyword(s):  
Activated Sludge ◽  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reduction Rate ◽  
Filamentous Bulking ◽  
Sulfate Reducing ◽  
Sulfur Bacteria ◽  
Sulfate Reduction Rates ◽  
Reducing Bacteria

The interactions between filamentous sulfur bacteria (FSB), sulfate reducing bacteria (SRB) and poly-P accumulating bacteria (PAB) in the activated sludge of a municipal plant operated under anaerobic-oxic conditions were examined in batch experiments using return sludge (RAS) and settled sewage. Phosphate release and sulfate reduction occurred simultaneously under anaerobic conditions. SRB were more sensitive to temperature changes than PAB. SRB played an important role in the decomposition of propionate to acetate. When the sulfate reduction rates were high, there was a tendency for the maximum release of phosphate also to be high. This was explained by the fact that PAB utilized the acetate produced by SRB. Sulfur oxidizing bacteria were sensitive to temperature change. When the sulfate reduction rate was high, the sulfide oxidizing rate was also high and filamentous bulking occurred. The results showed that sulfate reduction was a cause of filamentous bulking due to Type 021N that could utilize reduced sulfur.

scholarly journals Evaluation of Physiological Parameters of Intestinal Sulfate-Reducing Bacteria Isolated from Patients Suffering from IBD and Healthy People

2020 ◽  
Vol 9 (6) ◽  
pp. 1920 ◽  
Author(s):  
Ivan Kushkevych ◽  
Jorge Castro Sangrador ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Martin Černý ◽  
...  
Keyword(s):  
Sulfate Reduction ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Healthy Individuals ◽  
Fecal Samples ◽  
Sulfate Reducing ◽  
Cell Extracts ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

Background: Inflammatory bowel diseases (IBDs) are multifactorial illnesses of the intestine, to which microorganisms are contributing. Among the contributing microorganisms, sulfate-reducing bacteria (SRB) are suggested to be involved in the process of bowel inflammation due to the production of hydrogen sulfide (H2S) by dissimilatory sulfate reduction. The aims of our research were to physiologically examine SRB in fecal samples of patients with IBD and a control group, their identification, the study of the process of dissimilatory sulfate reduction (sulfate consumption and H2S production) and biomass accumulation. Determination of biogenic elements of the SRB and evaluation of obtained parameters by using statistical methods were also included in the research. The material for the research consisted of 14 fecal samples, which was obtained from patients and control subjects. Methods: Microscopic techniques, microbiological, biochemical, biophysical methods and statistical analysis were included. Results: Colonies of SRB were isolated from all the fecal samples, and subsequently, 35 strains were obtained. Vibrio-shaped cells stained Gram-negative were dominant in all purified studied strains. All strains had a high percentage of similarity by the 16S rRNA gene with deposited sequences in GenBank of Desulfovibrio vulgaris. Cluster analysis of sulfate reduction parameters allowed the grouping of SRB strains. Significant (p < 0.05) differences were not observed between healthy individuals and patients with IBD with regard to sulfate reduction parameters (sulfate consumption, H2S and biomass accumulation). Moreover, we found that manganese and iron contents in the cell extracts are higher among healthy individuals in comparison to unhealthy individuals that have an intestinal bowel disease, especially ulcerative colitis. Conclusions: The observations obtained from studying SRB emphasize differences in the intestinal microbial processes of healthy and unhealthy people.

Reduction of azo dyes by desulfovibrio desulfuricans

2000 ◽  
Vol 41 (12) ◽  
pp. 15-22 ◽  
Author(s):  
E. S. Yoo ◽  
J. Libra ◽  
U. Wiesmann
Keyword(s):  
Azo Dyes ◽  
Desulfovibrio Desulfuricans ◽  
Chemical Processes ◽  
Electron Acceptors ◽  
Potential Toxicity ◽  
Sulfate Reducing ◽  
Textile Finishing ◽  
Reactive Orange ◽  
Reducing Bacteria ◽  
Electron Carriers

Azo dyes are widely used in textile finishing, and have become of concern in wastewater treatment because of their color, bio-recalcitrance, and potential toxicity to animals and humans. Thus, wastewater with azo dyes must be decolorized and furthermore mineralized in appropriate systems combining biological and chemical processes. In this study, the potential for sulfate reducing bacteria (SRB) to decolorize azo dyes was studied, employing the pure culture of Desulfovibrio desulfuricans (D. desulfuricans) with varying sulfate levels. Under sulfate-rich conditions, the sulfide produced from sulfate respiration with pyruvate (electron donor) by D. desulfuricans chemically decolorized the azo dyes C. I. Reactive Orange 96 (RO 96) and C. I. Reactive Red 120 (RR 120). Under sulfate-depleted conditions (≤0.1 mmol/L), the decolorization of RO 96 and RR 120 occurred in correlation with the fermentation of pyruvate by D. desulfuricans. It is suggested that the electrons liberated from the pyruvate oxidation were transferred via enzymes and/or coenzymes (electron carriers) to the dyes as alternative terminal electron acceptors, giving rise to decolorization, instead of to the protons (H+), resulting in the production of H2. Both decolorization pathways were compared in light of bioenergetics and engineering aspects.

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