scholarly journals Pulsed-Field Gel Electrophoresis Analysis of Vibrio vulnificus Strains Isolated from Taiwan and the United States

2004 ◽  
Vol 70 (9) ◽  
pp. 5153-5158 ◽  
Author(s):  
Hin-chung Wong ◽  
Shau-Yan Chen ◽  
Meng-Yi Chen ◽  
James D. Oliver ◽  
Lien-I Hor ◽  
...  
Keyword(s):  
United States ◽  
Gel Electrophoresis ◽  
Vibrio Vulnificus ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Intraspecific Diversity ◽  
Pulsed Field ◽  
Clinical Strains ◽  
Discriminative Method ◽  
Geographic Regions

ABSTRACT Vibrio vulnificus is a marine bacterium that causes human wound infections and septicemia with a high mortality rate. V. vulnificus strains from different clinical and environmental sources or geographic regions have been successfully characterized by ribotyping and several other methods. Pulsed-field gel electrophoresis (PFGE) is a highly discriminative method, but previous studies suggested that it was not suitable for examining the correlation of V. vulnificus strains from different origins. We employed PFGE to determine its efficacy for characterizing V. vulnificus strains from different geographic regions, characterizing a total of 153 strains from clinical and environmental origins from the United States and Taiwan after SfiI or NotI digestion. V. vulnificus strains showed a high intraspecific diversity by PFGE after SfiI or NotI digestion, and about 12% of the strains could not be typed by the use of either of these enzymes. For PFGE with SfiI digestion, most of the clinical and environmental strains from the United States were grouped into cluster A, while the strains from Taiwan were grouped into other clusters. Clinical strains from the United States showed a higher level of genetic homogeneity than clinical strains from Taiwan, and environmental strains from both regions showed a similarly high level of heterogeneity. PFGE with NotI digestion was useful for studying the correlation of clinical strains from the United States and Taiwan, but it was not suitable for analyzing environmental strains. The results showed that PFGE with SfiI digestion may be used to characterize V. vulnificus strains from distant geographic regions, with NotI being a recommended alternative enzyme.

scholarly journals Comparison of Virulence Gene Identification, Ribosomal Spacer PCR, and Pulsed-Field Gel Electrophoresis for Typing of Staphylococcus aureus Strains Isolated from Cases of Subclinical Bovine Mastitis in the United States

2016 ◽  
Vol 54 (7) ◽  
pp. 1871-1876 ◽  
Author(s):  
Pamela R. F. Adkins ◽  
John R. Middleton ◽  
Lawrence K. Fox
Keyword(s):  
United States ◽  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Virulence Gene ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Gene Identification ◽  
Content Type ◽  
Pulsed Field

Staphylococcus aureusis one of the most important pathogens causing contagious mastitis in dairy cattle worldwide. The objectives of this study were to determine if recently describedS. aureusgenotype B was present among previously characterized isolates from cases of bovine intramammary infection in the United States and to compare pulsed-field gel electrophoresis (PFGE) to the combination of ribosomal spacer PCR (RS-PCR) and virulence gene identification for typing ofS. aureusstrains. The hypothesis was that isolates that were previously characterized as contagious would be identified as genotype B and that the results of the two strain-typing methods would be comparable. Isolates were selected from a collection ofS. aureusisolates from eight dairy farms. Mammary quarter milk somatic cell count (SCC) andN-acetyl-β-d-gluconaminidase (NAGase) activity data were known and used to evaluate strain pathogenicity. RS-PCR was performed with conventional gel electrophoresis, and PCR was used for toxin gene identification. RS-PCR patterns were associated with a specific virulence gene pattern, as previously reported. Five RS-PCR banding patterns were identified. None of the isolates were characterized as genotype B. No association between RS-PCR types and milk SCC was found; however, NAGase activity was significantly higher in milk from mammary glands infected with RS-PCR banding type 1 (RSP type 1) than in milk from those infected with RSP type 2. The discriminatory power values were 1.0 and 0.46 for PFGE and RS-PCR, respectively. These data suggest that genotype B may have a limited geographic distribution and that PFGE is more discriminatory than RS-PCR performed with conventional gel electrophoresis for typing ofS. aureusisolates of bovine origin.

Pulsed-field gel electrophoresis applied for comparing Listeria monocytogenes strains involved in outbreaks

1993 ◽  
Vol 39 (4) ◽  
pp. 395-401 ◽  
Author(s):  
C. Buchrieser ◽  
R. Brosch ◽  
B. Catimel ◽  
J. Rocourt
Keyword(s):  
United States ◽  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Chromosomal Dna ◽  
Restriction Patterns ◽  
Pulsed Field ◽  
Dna Restriction Fragments ◽  
Dna Restriction

Recent food-borne outbreaks of human listeriosis as well as numerous sporadic cases have been mainly caused by Listeria monocytogenes serovar 4b strains. Thus, it was of interest to find out whether a certain clone or a certain few clones were responsible for these cases and especially for outbreaks., We used pulsed-field gel electrophoresis of large chromosomal DNA restriction fragments generated by ApaI, SmaI, or NotI to analyse 75 L. monocytogenes strains isolated during six major and eight smaller recent listeriosis outbreaks. These strains could be divided into 20 different genomic varieties. Thirteen of 14 strains isolated during major epidemics in Switzerland (1983–1987), the United States (California, 1985) and Denmark (1985–1987) demonstrated indistinguishable DNA restriction patterns. In contrast, strains responsible for the outbreaks in Canada (Nova Scotia, 1981), the United States (Massachusetts, 1983), France (Anjou, 1975–1976), New Zealand (1969), and Austria (1986) and some smaller outbreaks in France (1987, 1988, 1989) were each characterized by particular combinations of DNA restriction patterns. Seventy-seven percent of the tested strains could be classified into the previously described ApaI group A (Brosch et al. 1991), demonstrating a very close genomic relatedness. Because 49% of the epidemic strains selected for this study belonged to phagovar 2389/2425/3274/2671/47/108/340 or 2389/47/108/340, fifty-six additional strains of these phagovars, isolated from various origins, were also typed to determine whether differences in DNA restriction profiles between epidemic and randomly selected strains of the same phagovars could be pointed out. Variations in DNA patterns appeared more frequently within randomly selected strains than within epidemic strains.Key words: Listeria monocytogenes, listeriosis, typing, pulsed-field gel electrophoresis, epidemic.

Characteristics of Salmonella Bareilly isolated from commercial layer farms and raw shell eggs in Korea

2019 ◽  
Vol 99 (2) ◽  
pp. 425-427
Author(s):  
Kwang Won Seo ◽  
Min Chan Im ◽  
Yeong Bin Kim ◽  
Haan Woo Sung ◽  
Young Ju Lee
Keyword(s):  
United States ◽  
Antimicrobial Resistance ◽  
Gel Electrophoresis ◽  
Geographical Variation ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Retail Markets ◽  
Pulsed Field ◽  
Shell Eggs ◽  
Epidemiological Characteristics

Salmonella enterica serovar Bareilly (S. Bareilly) has been among the top 20 most frequently isolated serovars in the United States and has been observed recently in layer flocks in Korea. Between 2013 and 2014, 45 S. Bareilly isolates were obtained from five commercial layer farms and nine retail markets in Korea. Among the 45 isolates, four pulsed-field gel electrophoresis patterns were observed, with pattern B being the predominant and comprising 67% of the 45 isolates. The most common antimicrobial resistance was streptomycin (24.4%) and cephalothin (6.7%). This is the first report describing epidemiological characteristics of S. Bareilly, including geographical variation, in Korea.

scholarly journals Use of Antibiotic Susceptibility Patterns and Pulsed-Field Gel Electrophoresis To Compare Historic and Contemporary Isolates of Multi-Drug-Resistant Salmonella enterica subsp. enterica Serovar Newport

2004 ◽  
Vol 70 (1) ◽  
pp. 318-323 ◽  
Author(s):  
Anna Catharina B. Berge ◽  
John M. Adaska ◽  
William M. Sischo
Keyword(s):  
United States ◽  
Antibiotic Resistance ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Samples ◽  
Genetic Lineage ◽  
Drug Resistant ◽  
Pulsed Field

ABSTRACT Recently, multi-drug-resistant (MDR) Salmonella enterica subspecies enterica serovar Newport reemerged as a public and animal health problem. The antibiotic resistance of 198 isolates and the pulsed-field gel electrophoresis patterns (PFGE) of 139 isolates were determined. Serovar Newport isolates collected between 1988 and 2001 were included in the study. One hundred seventy-eight isolates were collected from the San Joaquin valley in California and came from dairy cattle clinical samples, human clinical samples, bulk tank milk samples, fecal samples from preweaned calves, and waterways. Twenty clinical isolates from humans from various regions of the United States were also included in the study. Resistance to 18 antibiotics was determined using a disk diffusion assay. PFGE patterns were determined using a single enzyme (XbaI). The PFGE and antibiogram patterns were described using cluster analysis. Although the antibiotic resistance patterns of historic (1988 to 1995) and contemporary (1999 to 2001) isolates were similar, the contemporary isolates differed from the historic isolates by being resistant to cephalosporins and florfenicol and in their general sensitivity to kanamycin and neomycin. With few exceptions, the contemporary isolates clustered together and were clearly separated from the historic isolates. One PFGE-antibiogram cluster combination was predominant for the recent isolates, which were taken from human samples from all parts of the United States, as well as in the isolates from California, indicating a rapid dissemination of this phenotypic strain. The data are consistent with the hypothesis that the reemergence of MDR serovar Newport is not simply an acquisition of further antibiotic resistance genes by the historic isolates but reflects a different genetic lineage.

scholarly journals Relatedness of Listeria monocytogenes Isolates Recovered from Selected Ready-To-Eat Foods and Listeriosis Patients in the United States

2005 ◽  
Vol 71 (12) ◽  
pp. 8115-8122 ◽  
Author(s):  
Stefanie Evans Gilbreth ◽  
Jeff E. Call ◽  
F. Morgan Wallace ◽  
Virginia N. Scott ◽  
Yuhuan Chen ◽  
...  
Keyword(s):  
United States ◽  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Food Products ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Pulsed Field ◽  
Serotype 1 ◽  
Serotype 4B

ABSTRACT Pulsed-field gel electrophoresis and serotyping were performed for 544 isolates of Listeria monocytogenes, including 502 isolates recovered from contaminated samples from 31,705 retail ready-to-eat (RTE) food products and 42 isolates recovered from human cases of listeriosis. The isolates were from Maryland (294 isolates) and California (250 isolates) and were collected in 2000 and 2001. The isolates were placed into 16 AscI pulsogroups (level of relatedness within each group, ≥66%), 139 AscI pulsotypes (levels of relatedness, ≥25% to 100%), and eight serotypes (serotypes 1/2a, 1/2b, 1/2c, 3a, 3b, 4b, 4c, and 4d). The most frequently found pulsotypes belonged to either pulsogroup A (150 food isolates plus 4 clinical isolates) or pulsogroup B (104 food isolates plus 5 clinical isolates). The majority of the 502 food isolates were either serotype 1/2a (298 isolates) or serotype 1/2b (133 isolates), whereas the majority of the 42 clinical isolates were either serotype 1/2a (19 isolates) or serotype 4b (15 isolates). Additionally, 13 clinical isolates displayed pulsotypes also found in food isolates, whereas the remaining 29 clinical isolates displayed 24 unique pulsotypes. These data indicate that most (86%) of the L. monocytogenes subtypes found in the RTE foods sampled belonged to only two serotypes and that 90% of the isolates displayed 73 pulsotypes, with 107 isolates displaying pulsotype 1. These data should help define the distribution and relatedness of isolates found in RTE foods in comparison with isolates that cause listeriosis.

scholarly journals International Clone of Neisseriameningitidis Serogroup A with Tetracycline Resistance Due to tet(B)

2005 ◽  
Vol 49 (3) ◽  
pp. 1198-1200 ◽  
Author(s):  
Sharon A. Crawford ◽  
Kristin R. Fiebelkorn ◽  
Jan E. Patterson ◽  
James H. Jorgensen
Keyword(s):  
United States ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Tetracycline Resistance ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Drug Efflux ◽  
Pulsed Field ◽  
Efflux Mechanism

ABSTRACT Thirteen Neisseria meningitidis clinical isolates from Africa, Asia, and the United States for which the tetracycline MICs were elevated (≥8 μg/ml) were examined for 14 recognized resistance genes. Only the drug efflux mechanism encoded by tet(B) was detected. All isolates were in serogroup A, belonged to complex ST-5, and were closely related by pulsed-field gel electrophoresis analysis.

scholarly journals Outbreak of S. Enteritidis PT9C associated with consumption of raw almonds in the US: situation in countries participating in the Enter-net dedicated surveillance network

2004 ◽  
Vol 8 (23) ◽  
Author(s):  
I. S.T. Fisher
Keyword(s):  
United States ◽  
Gel Electrophoresis ◽  
Salmonella Enteritidis ◽  
The United States ◽  
Pulsed Field Gel Electrophoresis ◽  
Surveillance Network ◽  
Pulsed Field ◽  
The Us ◽  
Electrophoresis Pattern

An outbreak of Salmonella Enteritidis PT9C in the United States with a unique pulsed field gel electrophoresis pattern was notified to the Enter-net dedicated surveillance network

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