scholarly journals Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis

2001 ◽  
Vol 8 (1) ◽  
pp. 166-169 ◽  
Author(s):  
Henk L. Smits ◽  
C. K. Eapen ◽  
Sheela Sugathan ◽  
Mariamma Kuriakose ◽  
M. Hussein Gasem ◽  
...  
Keyword(s):  
Positive Result ◽  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Test Line ◽  
Lateral Flow ◽  
Immunoglobulin M ◽  
Lateral Flow Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Immunoglobulin ◽  
Human Sera

ABSTRACT An assay device for the rapid detection ofLeptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked immunosorbent assay routinely used in the serodiagnosis of leptospirosis. The sensitivity of the assay varied with the stage of the disease. The assay uses stabilized components and is simply performed by the addition of serum and sample fluid to the sample well of the assay device. The assay is read after 10 min, and a positive result is obtained when staining of the test line is observed.

scholarly journals Sensitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies Against Typhus Rickettsiae, Rickettsia prowazekii and Rickettsia typhi

1977 ◽  
Vol 6 (2) ◽  
pp. 101-110
Author(s):  
Sidney Halle ◽  
Gregory A. Dasch ◽  
Emilio Weiss
Keyword(s):  
Immunosorbent Assay ◽  
Complement Fixation ◽  
Differential Susceptibility ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rickettsia Prowazekii ◽  
Rickettsia Typhi ◽  
Titration Curves ◽  
Ether Extraction ◽  
Human Sera

An enzyme-linked immunosorbent assay (ELISA) has been developed for the titration of rickettsial antibodies in human and animal sera. Two preparations of soluble typhus-group antigens were obtained from Rickettsia typhi and Rickettsia prowazekii by ether extraction: a standard antigen from infected yolk sacs (YS antigen) and one free of yolk sac contaminants from Renografin-purified rickettsiae (PR antigen). Rabbit, mouse, and guinea pig sera were obtained by immunization with viable purified R. typhi or R. prowazekii . Human sera were obtained from individuals who had recovered from laboratory infections with either typhus rickettsia months or years previously. Goat-derived anti-immunoglobulins were conjugated to alkaline phosphatase with glutaraldehyde. Although the PR and YS antigens gave equivalent antibody titers in the complement fixation test, the PR antigen was clearly superior in the ELISA. With this antigen, the titration curves of all antisera were linear over a wider range of serum concentrations and the titers were higher than with the YS antigen. With YS and PR antigens, ELISA titers were higher than those obtained by complement fixation by one and two orders of magnitude, respectively. In human sera, immunoglobulin G and immunoglobulin M antibodies were demonstrated by their respective anti-immunoglobulins and by differential susceptibility to ethanethiol. ELISA titers showed some type specificity, whereas none was observed in complement fixation tests. The ELISA is highly sensitive, reproducible, and easily adaptable to the various requirements of clinical and research laboratories.

scholarly journals Evaluation of Hemagglutinin Protein-Specific Immunoglobulin M for Diagnosis of Measles by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Protein Produced in a High-Efficiency Mammalian Expression System

1998 ◽  
Vol 36 (12) ◽  
pp. 3509-3513 ◽  
Author(s):  
Fabienne B. Bouche ◽  
Nicolaas H. C. Brons ◽  
Sophie Houard ◽  
Francois Schneider ◽  
Claude P. Muller
Keyword(s):  
Recombinant Protein ◽  
Immunosorbent Assay ◽  
Measles Virus ◽  
High Efficiency ◽  
Expression System ◽  
Immunoglobulin M ◽  
Initial Time ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mammalian Expression ◽  
Specific Immunoglobulin

Recombinant hemagglutinin (H) of the measles virus (MV) expressed in a mammalian high-expression system based on the Semliki Forest virus replicon was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG in patients with acute-phase measles. One hundred twelve serum specimens from 70 patients with measles were analyzed. Case definition was based on a commercial IgM ELISA that utilizes MV-infected cells (MV-ELISA) (Enzygnost; Behring Diagnostics); the clinical criteria of the Centers for Disease Control and Prevention (Atlanta, Ga.); and/or the increase in hemagglutinin test titers, neutralization test titers, and levels of MV-specific IgG whenever paired sera were available. The initial time courses of the IgM signal after the onset of rash are similar in the H- and MV-ELISAs. On days 0 to 19, both ELISAs detected IgM in 67 of 68 (98.5%) sera. Average maximal levels of IgM seem to persist, however, about 10 days longer in the MV-ELISA (up to day 25) than in the H-ELISA (day 15). From days 20 to 29 and 30 to 59, the H-ELISA detected only 64.3 (9 of 14) and 19.2% (5 of 26), respectively, of sera that were IgM positive by MV-ELISA. At least up to day 30, the performance of the H-ELISA seemed to be similar to that reported for commercial ELISAs based on whole MV. Our results demonstrate that MV H-specific IgM can be used to diagnose most measles cases from a single serum specimen collected within 19 days after the onset of rash and that the recombinant protein used in this study is suitable for this purpose.

Development of Enzyme linked Immunosorbent Assay and Lateral Flow Immunoassay for the Rapid Detection of Dapsone in Foods

2021 ◽  
Author(s):  
Chuanlai Xu ◽  
xin guo ◽  
lu lin ◽  
Shanshan Song ◽  
aihong wu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Health ◽  
Immunosorbent Assay ◽  
Food Chain ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Animals

The abuse of dapsone (DDS) in food animals could result in its accumulation in humans through the food chain thus endangering human health. A sensitive monoclonal antibody (mAb) against DDS...

scholarly journals Optimization of a Human Papillomavirus-Specific Enzyme-Linked Immunosorbent Assay

2002 ◽  
Vol 9 (3) ◽  
pp. 577-582 ◽  
Author(s):  
Kevin L. Karem ◽  
Alysia C. Poon ◽  
Cynthia Bierl ◽  
Rosane Nisenbaum ◽  
Elizabeth Unger
Keyword(s):  
Human Papillomavirus ◽  
Immunosorbent Assay ◽  
Critical Evaluation ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Optimal Cutoff ◽  
Optimal Method ◽  
Specific Immunoglobulin ◽  
Human Sera ◽  
Evaluation Techniques

ABSTRACT A strategy was developed for the control, standardization, and critical evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of human papillomavirus-specific immunoglobulin G in human sera. Control human sera, polyclonal animal sera, and monoclonal antibodies were used to establish optimal assay parameters, including antigen coating, serum dilutions, and criteria for daily reproducibility, monitoring, and rejection of assays. Three evaluation techniques were used in parallel to define an optimal cutoff absorbance value that yields greater than 93% sensitivity and 98.5% specificity in the assay's ability to discriminate positive and negative control sera. This strategy provides an optimal method by which to determine cutoff absorbance values for ELISA.

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