scholarly journals Advances in nucleic acid-based detection methods.

1992 ◽  
Vol 5 (4) ◽  
pp. 370-386 ◽  
Author(s):  
M J Wolcott
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Detection Methods ◽  
Background Information ◽  
Clinical Settings ◽  
Chain Reaction ◽  
Detection Techniques ◽  
Amplification Technique ◽  
Polymerase Chain

Laboratory techniques based on nucleic acid methods have increased in popularity over the last decade with clinical microbiologists and other laboratory scientists who are concerned with the diagnosis of infectious agents. This increase in popularity is a result primarily of advances made in nucleic acid amplification and detection techniques. Polymerase chain reaction, the original nucleic acid amplification technique, changed the way many people viewed and used nucleic acid techniques in clinical settings. After the potential of polymerase chain reaction became apparent, other methods of nucleic acid amplification and detection were developed. These alternative nucleic acid amplification methods may become serious contenders for application to routine laboratory analyses. This review presents some background information on nucleic acid analyses that might be used in clinical and anatomical laboratories and describes some recent advances in the amplification and detection of nucleic acids.

Ribonuclease H-cleavable and recombinase-quenching fluorescent probes for the real-time detection of strand invasion based amplification

2019 ◽  
Vol 11 (43) ◽  
pp. 5568-5576
Author(s):  
Sonja Elf ◽  
Kevin E. Eboigbodin
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Fluorescent Probes ◽  
Nucleic Acid Amplification ◽  
Ribonuclease H ◽  
Chain Reaction ◽  
Amplification Method ◽  
Polymerase Chain ◽  
Strand Invasion

SIBA is an established nucleic acid amplification method that is used as an alternative to polymerase chain reaction (PCR).

scholarly journals La PCR e le sue varianti

2008 ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Polymorphism ◽  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Nucleic Acid Amplification ◽  
Genetic Identification ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Reference Tool

The book "La PCR e le sue varianti" is designed as a reference tool for those whose laboratory activities deal with methods based on nucleic acid amplification. The text provides the theoretical bases of the polymerase chain reaction (PCR) and its variants (e.g. RT-PCR, quantitative PCR, isothermic PCR) in a rapid and concise manner and describes the principal applications used for genetic identification and the study of genetic polymorphism, in the form of a protocol that can be easily consulted by the users.

scholarly journals Nucleic Acid Testing of SARS-CoV-2

2021 ◽  
Vol 22 (11) ◽  
pp. 6150
Author(s):  
Hee-Min Yoo ◽  
Il-Hwan Kim ◽  
Seil Kim
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Reverse Transcription ◽  
Diagnostic Methods ◽  
Detection Methods ◽  
Molecular Diagnostic ◽  
Nucleic Acid Testing ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA) are considered to be rapid and simple nucleic acid detection methods and were reviewed in this paper. Non-conventional molecular diagnostic methods including next-generation sequencing (NGS), CRISPR-based assays and nanotechnology are improving the accuracy and sensitivity of COVID-19 diagnosis. In this review, we also focus on standardization of SARS-CoV-2 nucleic acid testing and the activity of the National Metrology Institutes (NMIs) and highlight resources such as reference materials (RM) that provide the values of specified properties. Finally, we summarize the useful resources for convenient COVID-19 molecular diagnostics.

scholarly journals The discovery of Bungowannah virus – an example of the need for conventional and new technologies

2009 ◽  
Vol 30 (4) ◽  
pp. 138
Author(s):  
Peter D Kirkland
Keyword(s):  
Monoclonal Antibody ◽  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
New Technologies ◽  
Nucleic Acid Amplification ◽  
Chain Reaction ◽  
Agar Gel ◽  
Human Illness ◽  
Polymerase Chain ◽  
Flying Fox

A novel disease in pigs and another new virus ? so where is the flying fox connection? This was one of the first questions that many observers asked from the sidelines. In this instance there was no known connection with flying foxes, no suggestion of human illness but, as the investigation unravelled, a probable cause was identified ? an apparently new virus that had close connections to an important pig pathogen that is exotic to Australia. This virus was, however, so genetically different from its relatives that pan reactive polymerase chain reaction-based assays would not detect it. It was so different antigenically that pan reactive monoclonal antibody panels would react with it. Nevertheless, a combination of simple agar gel immunodiffusion tests for antibodies and sophisticated nucleic acid amplification and sequencing methods proved to be the keys to the recognition of this virus.

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