scholarly journals Use of the Brucella melitensis Native Hapten To Diagnose Brucellosis in Goats by a Rapid, Simple, and Specific Fluorescence Polarization Assay

2008 ◽  
Vol 15 (6) ◽  
pp. 911-915 ◽  
Author(s):  
Carlos Ramírez-Pfeiffer ◽  
Efrén Díaz-Aparicio ◽  
Ricardo Gomez-Flores ◽  
Cristina Rodríguez-Padilla ◽  
Alberto Morales-Loredo ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Fluorescence Polarization ◽  
Cell Concentration ◽  
Brucella Melitensis ◽  
Animal Health ◽  
Screening Tests ◽  
Confirmatory Test ◽  
Rose Bengal Test ◽  
Fluorescence Polarization Assay ◽  
The Rose

ABSTRACT The performance of the fluorescence polarization assay (FPA) using the recently described Brucella melitensis native hapten and the Brucella abortus O-polysaccharide tracer was evaluated and compared with those of The World Organization for Animal Health tests related to indirect and competitive enzyme-linked immunosorbent assays as classification variables for goat sera obtained from a high-prevalence area where vaccination was performed; test series were also evaluated to increase the final specificity of the tests. Our results showed that the respective relative sensitivity and specificity were 99.7% and 32.5% for the rose Bengal test with a 3% cell concentration (RBT3), 92.8% and 68.8% for the rose Bengal test with 8% cell concentration (RBT8), 98.4% and 84.9% for the Canadian complement fixation test (CFT), 83.7% and 65.5% for the Mexican CFT, 98.4% and 81.0% for the buffered plate agglutination test (BPAT), and 78.1% and 89.3% for the fluorescence polarization assay (FPA). The use of the FPA as the secondary test significantly increased the final specificities of test combinations; the screening tests BPAT, RBT3, and RBT8 plus FPA resulted in 90%, 91.2%, and 91.3% final specificities, respectively, whereas for the combinations RBT3 plus Mexican CFT, RBT8 plus Mexican CFT, and BPAT plus Canadian CFT, the specificities were 65.5%, 63.2%, and 91.7%, respectively. The results suggested that the FPA may be routinely applied as an adaptable screening test for diagnosis of goat brucellosis, since its cutoff can be adjusted to improve its sensitivity or specificity, it is a rapid and simple test, it can be the test of choice when specificity is relevant or when an alternative confirmatory test is not available, and it is not affected by vaccination, thus reducing the number of goats wrongly slaughtered due to misdiagnosis.

scholarly journals Application of the Fluorescence Polarization Assay for Detection of Caprine Antibodies to Brucella melitensis in Areas of High Prevalence and Widespread Vaccination

2007 ◽  
Vol 14 (3) ◽  
pp. 299-303 ◽  
Author(s):  
C. Ramírez-Pfeiffer ◽  
K. Nielsen ◽  
P. Smith ◽  
F. Marín-Ricalde ◽  
C. Rodríguez-Padilla ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Screening Test ◽  
Brucella Melitensis ◽  
Low Cost ◽  
Animal Health ◽  
Screening Tests ◽  
Confirmatory Test ◽  
High Prevalence ◽  
Fluorescence Polarization Assay ◽  
World Organization

ABSTRACT The screening Rose Bengal test (RBT), the buffered plate agglutination test (BPAT), and the confirmatory complement fixation test (CFT) are currently approved by the World Organization for Animal Health (OIE) for diagnosis of goat brucellosis. However, RBT (at 3% or 8% cell concentration) is known to be affected by vaccinal antibodies. In the present study, Mexican and Canadian OIE tests were compared with the fluorescence polarization assay (FPA), alone or in combination, using indirect and competitive enzyme-linked immunosorbent assays as classification variables for goat sera obtained from an area of high prevalence and widespread vaccination. The relative sensitivities and specificities were, respectively, 99.7% and 32.5% for RBT3, 92.8% and 68.8% for RBT8, 98.4% and 84.8% for Canadian CFT, 83.7% and 65.5% for Mexican CFT, and 78.1% and 89.3% for FPA. The use of FPA as the confirmatory test in combination with other tests significantly increased the final specificities of the screening tests alone; BPAT, RBT3, and RBT8 plus FPA resulted in final specificities of 90%, 91.2%, and 91.3%, respectively, whereas for the combinations RBT3 plus Mexican CFT, RBT8 plus Mexican CFT, and BPAT plus Canadian CFT, specificities were 65.5%, 63.2%, and 91.7%, respectively. We suggest that FPA may be routinely applied as an adaptable screening test for diagnosis of goat brucellosis and as a confirmatory test for screening test series. Some advantages of FPA are that its cutoff can be adjusted to improve its sensitivity or specificity, it is a low-cost and easy-to-perform test of choice when specificity is relevant or when an alternative confirmatory test is not available, and it is not affected by vaccination, thus reducing the number of misdiagnosed and killed goats.

scholarly journals The Rose Bengal Test in Human Brucellosis: A Neglected Test for the Diagnosis of a Neglected Disease

2011 ◽  
Vol 5 (4) ◽  
pp. e950 ◽  
Author(s):  
Ramón Díaz ◽  
Aurora Casanova ◽  
Javier Ariza ◽  
Ignacio Moriyón
Keyword(s):  
Rose Bengal ◽  
Human Brucellosis ◽  
Neglected Disease ◽  
Rose Bengal Test ◽  
The Rose

The Rose Bengal Test of Liver Function

1933 ◽  
Vol 3 (1) ◽  
pp. 55-60 ◽  
Author(s):  
W. Parker Stowe ◽  
G. D. Delprat ◽  
Alanson Weeks
Keyword(s):  
Liver Function ◽  
Rose Bengal ◽  
Rose Bengal Test ◽  
The Rose

scholarly journals The use of the milk ring test and rose bengal test in brucellosis control and eradication in Nigeria

2008 ◽  
Vol 79 (3) ◽  
Author(s):  
S.I.B. Cadmus ◽  
H.K. Adesokan ◽  
J. Stack
Keyword(s):  
Rose Bengal ◽  
Screening Tests ◽  
Ring Test ◽  
Serum Samples ◽  
Chi Square ◽  
Southwestern Nigeria ◽  
Rose Bengal Test ◽  
The Difference ◽  
Control And Eradication ◽  
Milk Ring Test

In this study, milk and blood samples collected simultaneously from 532 trade cows to be slaughtered at Bodija abattoir, Ibadan (southwestern, Nigeria) were examined for antibodies to Brucella using the milk ring test (MRT) and the rose bengal test (RBT). Overall, 18.61%of the milk samples were positive according to the MRT, while 9.77 % of the serum samples were positive according to the RBT. The difference was highly significant (Chi-square value 16.33; P<0.05); only 32 (6.02 %) of the samples were positive for both tests. The Red Bororo breed of cattle and the White Fulani had the highest positive rates, namely 20.93 % and 11.69%for the MRT and RBT respectively.No conclusion can be drawn about sensitivity because we do not know the true status of the animals tested. It is, however, obvious that although the MRT and RBT are 1st-line screening tests for brucellosis in cows in some countries, their lack of specificity is of concern. Therefore, the requirement for other confirmatory tests that are more specific should be considered for control and eradication of the disease, especially in Nigeria.

scholarly journals Serology for brucellosis in free-ranging crab-eating foxes (Cerdocyon thous) and brown-nosed coatis (Nasua nasua) from Brazilian Pantanal

2014 ◽  
Vol 44 (12) ◽  
pp. 2193-2196 ◽  
Author(s):  
Elaine Maria Seles Dorneles ◽  
Aiesca Oliveira Pellegrin ◽  
Igor Alexandre Hany Fuzeta Schabib Péres ◽  
Luis Antônio Mathias ◽  
Guilherme Mourão ◽  
...  
Keyword(s):  
Confirmatory Test ◽  
Serological Survey ◽  
Wild Populations ◽  
Nasua Nasua ◽  
Cerdocyon Thous ◽  
And Gender ◽  
Fluorescence Polarization Assay ◽  
Brazilian Pantanal ◽  
Free Ranging ◽  
The Rose

A serological survey in free-ranging crab-eating foxes (Canidae: Cerdocyon thous) and brown-nosed coatis (Procyonidae: Nasua nasua) was performed in the Nhecolândia sub-region of the Brazilian Pantanal to evaluate the presence of anti-smooth Brucella antibodies on those wild populations. The detection of anti-smooth Brucella antibodies was performed by the Rose Bengal Test (RBT) as screening test and the Fluorescence Polarization Assay (FPA) as a confirmatory test. The frequency of smooth Brucella seropositive animals were 13.2% (5/38, 95% CI: 4.4% - 28.1%) for crab-eating foxes and 8.8% (3/34, 95% CI: 1.9% -23.7%) for brown-nosed coatis. No association was found between seropositivity for brucellosis and gender or age. The results of this study suggest exposure to or infection of crab-eating fox and brown-nosed coati from the Brazilian Pantanal by Brucella spp

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