scholarly journals Antibody-Secreting Cell Responses after Vibrio cholerae O1 Infection and Oral Cholera Vaccination in Adults in Bangladesh

2013 ◽  
Vol 20 (10) ◽  
pp. 1592-1598 ◽  
Author(s):  
Atiqur Rahman ◽  
Rasheduzzaman Rashu ◽  
Taufiqur Rahman Bhuiyan ◽  
Fahima Chowdhury ◽  
Ashraful Islam Khan ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Cholera Toxin B Subunit ◽  
Secreting Cell ◽  
Toxin B ◽  
Content Type ◽  
Antibody Secreting Cell ◽  
B Subunit ◽  
Cell Responses ◽  
Vibrio Cholerae O1 ◽  
Cholera Vaccination

ABSTRACTInfection withVibrio choleraeand oral cholera vaccines (OCVs) induce transient circulating plasmablast responses that peak within approximately 7 days after infection or vaccination. We previously demonstrated that plasmablast responses strongly correlate with subsequent levels ofV. cholerae-specific duodenal antibodies up to 6 months afterV. choleraeinfection. Hence, plasmablast responses provide an early window into the immunologic memory at the mucosal surface. In this study, we characterized plasmablast responses followingV. choleraeinfection using a flow cytometrically defined population and comparedV. cholerae-specific responses in adult patients withV. choleraeO1 infection and vaccinees who received the OCV Dukoral (Crucell Vaccines Canada). Among flow cytometrically sorted populations of gut-homing plasmablasts, almost 50% of the cells recognized either cholera toxin B subunit (CtxB) orV. choleraeO1 lipopolysaccharide (LPS). Using a traditional enzyme-linked immunosorbent spot assay (ELISPOT), we found that infection withV. choleraeO1 and OCVs induce similar responses to the protein antigen CtxB, but responses to LPS were diminished after OCV compared to those after naturalV. choleraeinfection. A second dose of OCV on day 14 failed to boost circulatingV. cholerae-specific plasmablast responses in Bangladeshi adults. Our results differ from those in studies from areas where cholera is not endemic, in which a second vaccination on day 14 significantly boosts plasmablast responses. Given these results, it is likely that the optimal boosting strategies for OCVs differ significantly between areas whereV. choleraeinfection is endemic and those where it is not.

scholarly journals Antigen-Specific Memory B-Cell Responses in Bangladeshi Adults after One- or Two-Dose Oral Killed Cholera Vaccination and Comparison with Responses in Patients with Naturally Acquired Cholera

2011 ◽  
Vol 18 (5) ◽  
pp. 844-850 ◽  
Author(s):  
Mohammad Murshid Alam ◽  
M. Asrafuzzaman Riyadh ◽  
Kaniz Fatema ◽  
Mohammad Arif Rahman ◽  
Nayeema Akhtar ◽  
...  
Keyword(s):  
B Cell ◽  
Acute Stage ◽  
Cholera Toxin B Subunit ◽  
Memory B Cell ◽  
Content Type ◽  
Cell Responses ◽  
Specific Memory ◽  
Dose Oral ◽  
B Cell Responses ◽  
Cholera Vaccination

ABSTRACTThe mediators of protective immunity against cholera are currently unknown, but memory B-cell responses may play a central role in facilitating long-term and anamnestic responses againstVibrio cholerae, the cause of cholera. We compared memory B-cell responses in adults with natural cholera in Bangladesh (n= 70) to responses in Bangladeshi adults after one-dose (n= 30) or two-dose (n= 30) administration of an oral killed cholera vaccine, WC-rBS (Dukoral; Crucell), assessing the responses at the acute stage of disease or prevaccination and then on days 3, 30, 90, 180, 270, and 360. Individuals with natural cholera developed prominent vibriocidal and plasma anti-cholera toxin B subunit (CtxB) and lipopolysaccharide (LPS) IgG and IgA responses, but these responses returned to baseline by 1 year of follow-up. Vaccinees developed plasma anti-CtxB and anti-LPS IgG and IgA responses that were generally comparable to those in individuals recovering from natural disease, but vibriocidal responses were lower in vaccinees than in infected patients. Individuals recovering from natural disease developed memory B-cell IgG and IgA anti-CtxB and anti-LPS responses by day 30, and these responses were detectable through at least days 180 to 360. In contrast, we detected no IgA or IgG memory B-cell responses to LPS in vaccinees; anti-CtxB IgA responses were only detectable on day 30, and anti-CtxB IgG responses were detectable until days 90 to 180, compared to days 270 to 360 in patients. These findings may explain in part the relatively short-term protection afforded by oral cholera vaccination compared to natural disease.

scholarly journals Induction and Distribution of Intestinal Immune Responses after Administration of Recombinant Cholera Toxin B Subunit in the Ileal Pouches of Colectomized Patients

2001 ◽  
Vol 69 (5) ◽  
pp. 3466-3471 ◽  
Author(s):  
Jan Kilhamn ◽  
Hans Brevinge ◽  
Marianne Quiding-Järbrink ◽  
Ann-Mari Svennerholm ◽  
Marianne Jertborn
Keyword(s):  
Immune Responses ◽  
Cholera Toxin ◽  
Peripheral Blood ◽  
Ileal Pouch ◽  
Immunoglobulin A ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Antibody Secreting Cell ◽  
Cholera Toxin B ◽  
B Subunit

ABSTRACT The induction and dissemination of mucosal immune responses to recombinant cholera toxin B subunit (rCTB) administered into the ileal pouches of patients, who had been colectomized because of ulcerative colitis, was analyzed. Biopsies from the duodenum and ileal pouch were collected, along with peripheral blood and ileostomy fluids. Two immunizations induced strong CTB-specific immunoglobulin A (IgA) antibody-secreting cell (ASC) responses in the duodenum in five of five patients, whereas weaker and less-frequent ASC responses were noted in the ileal pouch. Intestine-derived CTB-specific IgA ASCs were found in peripheral blood in three of the five patients. The vaccination also induced significant IgA antitoxin titer rises in ileostomy fluid in all of the patients. Increased production of gamma interferon in cell cultures from the ileal pouch was found in four of five patients after the vaccination. These results clearly indicate that rCTB administered into the distal ileum is capable of inducing B-cell responses in the “entire” small intestine and that homing of immunocompetent cells occurs preferentially to the duodenum.

scholarly journals Cytotoxic T cell responses are enhanced by antigen design involving the presentation of MUC1 peptide on cholera toxin B subunit

Oncotarget ◽  
2015 ◽  
Vol 6 (33) ◽  
pp. 34537-34548 ◽  
Author(s):  
Wuguang Lu ◽  
Lingchong Qiu ◽  
Zhanpeng Yan ◽  
Zhibing Lin ◽  
Meng Cao ◽  
...  
Keyword(s):  
T Cell ◽  
Cholera Toxin ◽  
T Cell Responses ◽  
Cytotoxic T Cell ◽  
Cholera Toxin B Subunit ◽  
Toxin B ◽  
Cholera Toxin B ◽  
B Subunit ◽  
Cell Responses ◽  
Cytotoxic T Cell Responses
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