scholarly journals Virus-Like Particle Vaccine Containing Hemagglutinin Confers Protection against 2009 H1N1 Pandemic Influenza

2011 ◽  
Vol 18 (12) ◽  
pp. 2010-2017 ◽  
Author(s):  
M. Jaber Hossain ◽  
Melissa Bourgeois ◽  
Fu-Shi Quan ◽  
Aleksandr S. Lipatov ◽  
Jae-Min Song ◽  
...  
Keyword(s):  
H1n1 Virus ◽  
Protective Efficacy ◽  
Influenza Pandemic ◽  
H1n1 Influenza ◽  
World Population ◽  
Promising Alternative ◽  
Virus Challenge ◽  
Virus Like Particle ◽  
Current Vaccine ◽  
2009 H1n1

ABSTRACTImmunization of the world population before an influenza pandemic such as the 2009 H1N1 virus spreads globally is not possible with current vaccine production platforms. New influenza vaccine technologies, such as virus-like-particles (VLPs), offer a promising alternative. Here, we tested the immunogenicity and protective efficacy of a VLP vaccine containing hemagglutinin (HA) and M1 from the 2009 pandemic H1N1 influenza virus (H1N1pdm) in ferrets and compared intramuscular (i.m.) and intranasal (i.n.) routes of immunization. Vaccination of ferrets with VLPs containing the M1 and HA proteins from A/California/04/2009 (H1N1pdm) induced high antibody titers and conferred significant protection against virus challenge. VLP-vaccinated animals lost less weight, shed less virus in nasal washes, and had markedly lower virus titers in all organs tested than naïve controls. A single dose of VLPs, either i.m. or i.n., induced higher levels of antibody than did two doses of commercial split vaccine. Ferrets vaccinated with split vaccine were incompletely protected against challenge; these animals had lower virus titers in olfactory bulbs, tonsils, and intestines, but lost weight and shed virus in nasal washes to a similar extent as naïve controls. Challenge with heterologous A/Brisbane/59/07 (H1N1) virus revealed that the VLPs conferred minimal cross-protection to heterologous infection, as revealed by the lack of reduction in nasal wash and lung virus titers and slightly higher weight loss relative to controls. In summary, these experiments demonstrate the strong immunogenicity and protective efficacy of VLPs compared to the split vaccine and show that i.n. vaccination with VLPs has the potential for highly efficacious vaccination against influenza.

scholarly journals The PB2-E627K Mutation Attenuates Viruses Containing the 2009 H1N1 Influenza Pandemic Polymerase

mBio ◽  
2010 ◽  
Vol 1 (1) ◽  
Author(s):  
Brett W. Jagger ◽  
Matthew J. Memoli ◽  
Zong-Mei Sheng ◽  
Li Qi ◽  
Rachel J. Hrabal ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
H1n1 Virus ◽  
Influenza Pandemic ◽  
Influenza Infection ◽  
H1n1 Influenza ◽  
Influenza Viruses ◽  
Pandemic Virus ◽  
2009 H1n1 ◽  
Animal Reservoirs

ABSTRACTThe swine-origin H1N1 influenza A virus emerged in early 2009 and caused the first influenza pandemic in 41 years. The virus has spread efficiently to both the Northern and the Southern Hemispheres and has been associated with over 16,000 deaths. Given the virus’s recent zoonotic origin, there is concern that the virus could acquire signature mutations associated with the enhanced pathogenicity of previous pandemic viruses or H5N1 viruses with pandemic potential. We tested the hypothesis that mutations in the polymerase PB2 gene at residues 627 and 701 would enhance virulence but found that influenza viruses containing these mutations in the context of the pandemic virus polymerase complex are attenuated in cell culture and mice.IMPORTANCEInfluenza A virus (IAV) evolution is characterized by host-specific lineages, and IAVs derived in whole or in part from animal reservoirs have caused pandemics in humans. Because IAVs are known to acquire host-adaptive genome mutations, and since the PB2 gene of the 2009 H1N1 virus is of recent avian derivation, there exists concern that the pathogenicity of the 2009 H1N1 influenza A pandemic virus could be potentiated by acquisition of the host-adaptive PB2-E627K or -D701N mutations, which have been shown to enhance the virulence of other influenza viruses. We present data from a mouse model of influenza infection showing that such mutations do not increase the virulence of viruses containing the 2009 H1N1 viral polymerase.

scholarly journals Subcutaneous Immunization with Baculovirus Surface-Displayed Hemagglutinin of Pandemic H1N1 Influenza A Virus Induces Protective Immunity in Mice

2011 ◽  
Vol 18 (9) ◽  
pp. 1582-1585 ◽  
Author(s):  
Mookkan Prabakaran ◽  
Tao Meng ◽  
Fang He ◽  
Tan YunRui ◽  
Jia Qiang ◽  
...  
Keyword(s):  
Virus Infection ◽  
Protective Immunity ◽  
Influenza A ◽  
H1n1 Virus ◽  
H1n1 Influenza ◽  
Influenza Virus Hemagglutinin ◽  
Inactive Form ◽  
2009 H1n1 ◽  
Subcutaneous Immunization ◽  
H1n1 Virus Infection

ABSTRACTThe protective immunity of baculovirus displaying influenza virus hemagglutinin (BacHA) against influenza 2009 H1N1 virus infection in a murine model was investigated. The results showed that mice vaccinated with live BacHA or an inactive form of adjuvanted BacHA had enhanced specific antibody responses and induced protective immunity against 2009 H1N1 virus infection, suggesting the potential of baculovirus as a live or inactivated vaccine.

scholarly journals Clinical and economic analysis of the 2009 H1N1 influenza pandemic among pregnant Korean women

2019 ◽  
Vol 34 (5) ◽  
pp. 1136-1144
Author(s):  
Won Suk Choi ◽  
Min Joo Choi ◽  
Ji Yoon Noh ◽  
Joon Young Song ◽  
Woo Joo Kim ◽  
...  
Keyword(s):  
Economic Analysis ◽  
Influenza Pandemic ◽  
H1n1 Influenza ◽  
Korean Women ◽  
2009 H1n1 ◽  
H1n1 Influenza Pandemic

scholarly journals The Real Impact of the Coronavirus Disease 2019 (covid-19) on the Pregnancy Outcome

2020 ◽  
Vol 42 (05) ◽  
pp. 303-304
Author(s):  
Ana Katherine Gonçalves
Keyword(s):  
Pregnant Women ◽  
Health Care Workers ◽  
Influenza Pandemic ◽  
The Elderly ◽  
H1n1 Influenza ◽  
Clinical Impact ◽  
Additional Information ◽  
Intrauterine Transmission ◽  
Care Workers ◽  
2009 H1n1

AbstractThe COVID-19 outbreak is increasing around the world in the number of cases, deaths, and affected countries. Currently, the knowledge regarding the clinical impact of COVID-19 on maternal, fetal, and placental aspects of pregnancy is minimal. Although the elderly and men were the most affected population, in previous situations, such as the 2009 H1N1 influenza pandemic and the Ebola epidemic, pregnant women were more likely to develop complications than nonpregnant women. There are unanswered questions specific to pregnant women, such as whether pregnant women are more severely affected and whether intrauterine transmission occurs. Additional information is needed to inform key decisions, such as whether pregnant health care workers should receive special consideration, whether to separate infected mothers and their newborns, and whether it is safe for infected women to breastfeed.

scholarly journals Origins of the 2009 H1N1 influenza pandemic in swine in Mexico

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Ignacio Mena ◽  
Martha I Nelson ◽  
Francisco Quezada-Monroy ◽  
Jayeeta Dutta ◽  
Refugio Cortes-Fernández ◽  
...  
Keyword(s):  
Influenza A ◽  
Influenza Pandemic ◽  
H1n1 Influenza ◽  
Central Mexico ◽  
Genome Sequences ◽  
Viral Reservoirs ◽  
Long Distance ◽  
Conflicting Evidence ◽  
2009 H1n1 ◽  
H1n1 Influenza Pandemic

Asia is considered an important source of influenza A virus (IAV) pandemics, owing to large, diverse viral reservoirs in poultry and swine. However, the zoonotic origins of the 2009 A/H1N1 influenza pandemic virus (pdmH1N1) remain unclear, due to conflicting evidence from swine and humans. There is strong evidence that the first human outbreak of pdmH1N1 occurred in Mexico in early 2009. However, no related swine viruses have been detected in Mexico or any part of the Americas, and to date the most closely related ancestor viruses were identified in Asian swine. Here, we use 58 new whole-genome sequences from IAVs collected in Mexican swine to establish that the swine virus responsible for the 2009 pandemic evolved in central Mexico. This finding highlights how the 2009 pandemic arose from a region not considered a pandemic risk, owing to an expansion of IAV diversity in swine resulting from long-distance live swine trade.

scholarly journals Resource Burden at Children’s Hospitals Experiencing Surge Volumes During the Spring 2009 H1N1 Influenza Pandemic

2011 ◽  
Vol 18 (2) ◽  
pp. 158-166 ◽  
Author(s):  
Marion R. Sills ◽  
Matthew Hall ◽  
Harold K. Simon ◽  
Evan S. Fieldston ◽  
Nicholas Walter ◽  
...  
Keyword(s):  
Influenza Pandemic ◽  
H1n1 Influenza ◽  
Children's Hospitals ◽  
Children’S Hospitals ◽  
2009 H1n1 ◽  
H1n1 Influenza Pandemic
Sign in / Sign up

Export Citation Format

Share Document