Giardia duodenalis in Wildlife: Exploring Genotype Diversity in Italy and across Europe

Fragmented data are so far available on genotype diversity of G. duodenalis in wildlife in different countries in Europe, in particular, in Italy. In the present study, G. duodenalis sequences obtained from different Italian wild animals [12 porcupines (Hystrix cristata), 4 wild boars (Sus scrofa), 1 wolf (Canis lupus italicus), 6 Alpine chamois (Rupicapra rupicapra rupicapra)] were compared with those available from wild host species in Europe to add new data on the geographic distribution of Giardia assemblages/sub-assemblages and their transmission patterns among natural hosts. Thirty-eight sequences were obtained by MLG analysis (SSU-rRNA, bg, gdh, and tpi genes) and subsequently compared by phylogenetic and network analyses with those from wild species monitored in the last decades in Europe. The results revealed the presence of potentially zoonotic (A-AI, A-AII from wild boar; B from porcupine) and host-adapted (D from wolf; E, A-AIII from chamois) assemblages and sub-assemblages and represent the first report for Italian wild boar. The analysis did not find any evidence of spatial or host segregation for specific genetic variants, mostly shared between different hosts from different European countries. However, conflicting evidence was found in genotypic assignment, advocating for data improvement and new genomic approaches.

An Evidence Combination Rule Based on New Weight Assignment Scheme

Conflicting evidence and fuzzy evidence have a significant impact on the results of evidence combination in the application of evidence theory. However, the existing weight assignment methods can hardly reflect the significant influence of fuzzy evidence on the combination results. Therefore, a new method for assigning evidence weights and the corresponding combination rule are proposed. The proposed weight assignment method strengthens the consideration of fuzzy evidence and introduces the Wasserstein distance to compute the clarity degree of evidence which is an important reference index for weight assignment in the proposed combination rule and can weaken the effect of ambiguous evidence effectively. In the experiments, it's firstly verified that the impact of fuzzy evidence on the combination results is significant; therefore it should be fully considered in the weight assignment process. Then, the proposed combination rule with new weight assignment method is tested on a set of numerical arithmetic and Iris datasets. Compared with four existing methods, the results show that the proposed method has higher decision accuracy, F1 score, better computational convergence, and more reliable fusion results as well.

A new method of conflicting evidence management based on non-extensive entropy and Lance distance in uncertain scenarios

Handing uncertain information is one of the research focuses currently. For the sake of great ability of handing uncertain information, Dempster-Shafer evidence theory (D-S theory) has been widely used in various fields of uncertain information processing. However, when highly contradictory evidence appears, the results of the classical Dempster combination rules (DCR) can be counterintuitive. Aiming at this defect, by considering the relationship between the evidence and its own characteristics, the proposed method is a new method of conflicting evidence management based on non-extensive entropy and Lance distance in uncertain scenarios. Firstly, the Lance distance function is used to measure the degree of discrepancy and conflict between evidences, and the credibility of evidence is expressed by matrix. Introducing non-extensive entropy to measure the amount of information about evidence and express the uncertainty of evidence. Secondly, the discount coefficient of the final fusion evidence is measured by considering the credibility and uncertainty of the evidence, and the original evidence is modified by the discount coefficient. Then, the final result is obtained by evidence fusion with DCR. Finally, two numerical examples are provided to illustrate the efficiency of the proposed method, and the utility of our work is demonstrated through an application of the active lane change to avoid obstacles to the autonomous driving of new energy vehicles. The proposed method has a better identification accuracy, reaching 0.9811.

The Effect of Optical Defocus on the Choroidal Thickness: A Review

The choroid is a heavily vascularized tissue located between the retina and sclera and plays a primary role in ocular metabolism. It has recently been suggested that the choroid has the ability to change its thickness and secretion of growth factors. This may play an important role during visual development by adjusting retinal position during growth to support emmetropisation; however, the mechanism by which changes in choroidal thickness (ChT) occur is unclear. This relationship becomes an interesting topic in the clinical field, although conflicting evidence found that these changes in the choroidal thickness may not be associated with the development of refractive errors. Many reports have investigated the changes in the choroid and related factors that affect the ChT. Thus, this review will summarize the current literature related to choroidal thickness in different refractive error groups, determine the factors that influence the thickness of the choroid, and discuss in detail the relationship between the changes in the ChT and ocular elongation, and therefore, the effect of optical defocus on ChT and the development of the refractive error.

Partisan Strategy and the Adoption of Same-Day Registration in the American States

This study seeks to explain state adoptions of same-day registration (SDR), with a focus on determining whether the Democratic (Republican) Party’s support of (resistance to) this impactful voting reform is driven by strategic electoral considerations. I find that states have an increased probability of enacting the reform when legislative Democrats are in the precarious position that comes with having just experienced minority status in one or both chambers. Relatedly, I demonstrate that the presence of a Republican legislature does not make adoption less likely until the size of the Black population reaches a certain threshold. In fact, provided the Black population is small enough, Republican control of the legislature encourages reform. The results offer conflicting evidence, however, that large Latino populations deter the GOP from establishing SDR. Considered together, the results cast doubt on the claim that either party’s position is informed by principle alone.

Late onset congenital adrenal hyperplasia in pregnancy

Congenital adrenal Hyperplasia (CAH) is a rare disorder to manage in pregnancy as CAH is known to cause infertility. Late onset CAH is more so with 21-hydroxylase deficiency being the most common enzyme deficiency for the same. The mainstay of management in pregnancy is multidisciplinary team management with a consultant Obstetrician and Medical Endocrinologist, steroid treatment and avoiding virilisation of the female patient in early pregnancy is important continuation of dexamethasone is controversial with conflicting evidence and also precipitating or worsening hyperemesis in pregnancy.

Touched by Loneliness – How Loneliness Impacts the Response to Observed Human Touch: a tDCS Study

Lonely people often crave connectedness. However, they may also experience their environment as threatening, entering a self-preserving state that perpetuates loneliness. Research shows conflicting evidence about their response to positive social cues, and little is known about their experience of observed human touch. The right inferior frontal gyrus (rIFG) is part of an observation-execution network implicated in observed touch perception. Correlative studies also point to rIFG’s involvement in loneliness. We examined the causal effect of rIFG anodal transcranial direct current stimulation (tDCS) on high and low loneliness individuals observing human touch. In a cross-over design study, 40 participants watched pictures of humans or objects touching or not touching during anodal and sham stimulations. Participants indicated whether pictures contained humans or objects and their reaction time was measured. Results show that the reaction time of low loneliness individuals to observed human touch was significantly slower during anodal stimulation compared to high loneliness individuals, possibly due to them being more emotionally distracted by it. Lonely individuals also reported less liking of touch. Our findings support the notion that lonely individuals are not drawn to positive social cues. This may help explain the perpetuation of loneliness, despite social opportunities that could be available to lonely people.