scholarly journals Added Value of Use of a Purified Protein Derivative-Based Enzyme-Linked Immunosorbent Spot Assay for Patients with Mycobacterium bovis BCG Infection after Intravesical BCG Instillations

2012 ◽  
Vol 19 (6) ◽  
pp. 974-977 ◽  
Author(s):  
Karen A. Heemstra ◽  
Ailko W. J. Bossink ◽  
Roan Spermon ◽  
John J. M. Bouwman ◽  
Robert van der Kieft ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Immune Activation ◽  
Bladder Carcinoma ◽  
Case Series ◽  
Gamma Interferon ◽  
Added Value ◽  
Content Type ◽  
Mycobacterium Bovis Bcg ◽  
Purified Protein Derivative ◽  
Intravesical Bcg

ABSTRACTIn this case series, we describe four cases in which the use of gamma interferon release assays with purified protein derivative (PPD) as a stimulating antigen was able to demonstrate PPD-specific immune activation. This may help to improve the adequate diagnosis of (systemic)Mycobacterium bovisBCG infections after intravesical BCG instillations for bladder carcinoma.

scholarly journals Evaluation of Gamma Interferon (IFN-γ)-Induced Protein 10 Responses for Detection of Cattle Infected with Mycobacterium bovis: Comparisons to IFN-γ Responses

2012 ◽  
Vol 19 (3) ◽  
pp. 346-351 ◽  
Author(s):  
W. R. Waters ◽  
T. C. Thacker ◽  
B. J. Nonnecke ◽  
M. V. Palmer ◽  
I. Schiller ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Similar Pattern ◽  
Gamma Interferon ◽  
Content Type ◽  
Purified Protein Derivative ◽  
Testing Protocol ◽  
Archived Samples ◽  
Ifn Γ ◽  
Highly Correlated ◽  
Mycobacterial Antigens

ABSTRACTGamma interferon (IFN-γ)-induced protein 10 (IP-10) has recently shown promise as a diagnostic biomarker ofMycobacterium tuberculosisinfection of humans. The aim of the current study was to compare IP-10 and IFN-γ responses uponMycobacterium bovisinfection in cattle by using archived samples from two aerosol inoculation studies. In the first study (104CFUM. bovisby aerosol,n= 7),M. bovispurified protein derivative (PPDb)-specific IP-10 and IFN-γ gene expression was detected as early as 29 days after challenge. PPDb-specific IP-10 and IFN-γ mRNA responses followed a similar pattern of expression over the course of this study and were highly correlated (r= 0.87). In the second study (105CFUM. bovisby aerosol,n= 5), IP-10 and IFN-γ (protein) responses to mycobacterial antigens were compared following challenge. IFN-γ responses to mycobacterial antigens were detected at 29 days after challenge and were sustained during the remainder of the study. IFN-γ responses to mycobacterial antigens exceeded corresponding responses in nonstimulated cultures. IP-10 responses to mycobacterial antigens exceeded preinfection responses at 7, 29, and 63 days after challenge. In contrast to IFN-γ responses, IP-10 responses to mycobacterial antigens generally did not exceed the respective responses in nonstimulated cultures. IP-10 responses to medium alone and to mycobacterial antigens followed a similar pattern of response. Correlations between IP-10 and IFN-γ (protein) responses were modest (r≈ 0.50 to 0.65). Taken together, these findings do not support the use of IP-10 protein as a biomarker for bovine tuberculosis using the current testing protocol and reagents; however, mRNA-based assays may be considered for further analysis.

scholarly journals Recombinant Mycobacterium bovis BCG secreting functional interleukin-2 enhances gamma interferon production by splenocytes.

1994 ◽  
Vol 62 (6) ◽  
pp. 2508-2514 ◽  
Author(s):  
M A O'Donnell ◽  
A Aldovini ◽  
R B Duda ◽  
H Yang ◽  
A Szilvasi ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Interleukin 2 ◽  
Gamma Interferon ◽  
Interferon Production ◽  
Mycobacterium Bovis Bcg

scholarly journals Effects of Serial Skin Testing with Purified Protein Derivative on the Level and Quality of Antibodies to Complex and Defined Antigens in Mycobacterium bovis-Infected Cattle

2015 ◽  
Vol 22 (6) ◽  
pp. 641-649 ◽  
Author(s):  
W. Ray Waters ◽  
Mitchell V. Palmer ◽  
Molly R. Stafne ◽  
Kristin E. Bass ◽  
Mayara F. Maggioli ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Specific Antibody ◽  
Skin Testing ◽  
Antibody Responses ◽  
Proteinase K ◽  
Skin Tests ◽  
Infected Cattle ◽  
Content Type ◽  
Purified Protein Derivative ◽  
In Series

ABSTRACTSeveral serological tests designed to detect antibodies to immunodominantMycobacterium bovisantigens have recently emerged as ancillary tests for the detection of bovine tuberculosis in cattle, particularly when used after the injection of purified protein derivative (PPD) for skin testing, which significantly boostsM. bovis-specific antibody responses. The present findings demonstrate the onset and duration of boosted antibody responses after the injection ofM. bovisPPD for the caudal fold test (CFT) andMycobacterium aviumandM. bovisPPDs for the comparative cervical test (CCT), administered in series in cattle experimentally infected withM. bovis. While skin tests boosted the responses to certain antigens (i.e., MPB83 and MPB70), they did not affect the responses to other antigens (e.g., ESAT-6, CFP10, MPB59, and MPB64). Administration of the CCT 105 days after the CFT resulted in an even greater secondary boost in antibody responses to MPB83 and MPB70 and to a proteinase K-digested whole-cell sonicate (WCS-PK) ofM. bovis. Both IgM and IgG contributed to the initial boost in the MPB83/MPB70-specific antibody response after the CFT. The secondary boost after the CCT was primarily due to increased IgG levels. Also, the avidity of antibodies to MPB83 and MPB70 increased after the CCT inM. bovis-infected cattle. The avidity of antibodies to the WCS-PK antigens increased in the interval between the CFT and the CCT but did not increase further after the CCT. Together, these findings demonstrate that the administration of PPDs for skin tests results in additive enhancement (i.e., when the CFT and CCT are performed in series), both qualitative and quantitative, of MPB83/MPB70-specific antibody responses.

scholarly journals Increased Numbers of ESAT-6- and Purified Protein Derivative-Specific Gamma Interferon-Producing Cells in Subclinical and Active Tuberculosis Infection

2000 ◽  
Vol 68 (10) ◽  
pp. 6073-6076 ◽  
Author(s):  
Timo Ulrichs ◽  
Peter Anding ◽  
Steven Porcelli ◽  
Stefan H. E. Kaufmann ◽  
Martin E. Munk
Keyword(s):  
Mycobacterium Bovis ◽  
Synthetic Peptides ◽  
Gamma Interferon ◽  
Active Tuberculosis ◽  
Tuberculosis Infection ◽  
Potential Candidate ◽  
Tuberculosis Patients ◽  
Purified Protein Derivative ◽  
Healthy Donors ◽  
Ifn Γ

ABSTRACT Numbers of gamma interferon (IFN-γ)-producing cells reactive to ESAT-6 antigen were increased in recent converters to purified protein derivative positivity and in tuberculosis patients but not in unvaccinated or Mycobacterium bovis BCG-vaccinated healthy donors. ESAT-6-reactive IFN-γ-producing cells in recent converters and tuberculosis patients recognized similar synthetic peptides. Thus, ESAT-6 is a potential candidate for use in detection of early, as well as active, tuberculosis and for control of the disease.

scholarly journals Complete Genome Sequence of Mycobacterium bovis BCG SL222 Sofia, the First WHO Reference Reagent for the M. bovis BCG Vaccine of the Russian BCG-I Substrain

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Stefan Panaiotov ◽  
Yordan Hodzhev ◽  
Vladimir Tolchkov ◽  
Alexander Mihailov ◽  
Roumen Kofinov ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Vaccine Strain ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Bcg Vaccine ◽  
Vaccine Production ◽  
Content Type ◽  
Mycobacterium Bovis Bcg

ABSTRACT Mycobacterium bovis BCG SL222 Sofia is a substrain descending from the Russian BCG-I vaccine strain. Here, we report the complete genome sequence of BCG SL222 Sofia, which will facilitate identity assurance and will contribute to more consistent manufacturing, standardization, and differentiation of substrains used in vaccine production.

scholarly journals Evaluation of Gamma Interferon Release Assays Using Mycobacterium tuberculosis Antigens for Diagnosis of Latent and Active Tuberculosis in Mycobacterium bovis BCG-Vaccinated Populations

2010 ◽  
Vol 17 (12) ◽  
pp. 1985-1990 ◽  
Author(s):  
Shu Zhang ◽  
Lingyun Shao ◽  
Ling Mo ◽  
Jiazhen Chen ◽  
Feifei Wang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Cell ◽  
Mycobacterium Bovis ◽  
Gamma Interferon ◽  
Sputum Smear ◽  
Purified Protein Derivative ◽  
Tb Treatment ◽  
Latent Tb ◽  
Specific Antigens ◽  
Latent Tb Infection

ABSTRACT T-cell-based gamma interferon (IFN-γ) release assays (IGRAs) using Mycobacterium tuberculosis-specific antigens have shown higher sensitivity and specificity than the routine tuberculin skin test (TST). However, the effects of Mycobacterium bovis BCG vaccination and anti-tuberculosis (TB) treatment on dynamic T-cell responses to M. tuberculosis-specific antigens in active TB cases have rarely been investigated in regions where TB is endemic. Eighty-nine patients with active pulmonary TB (ATB) and 57 healthy controls (HC) from China were recruited and tested by sputum smear and culture, TSTs, and IGRAs with M. tuberculosis-specific antigens ESAT-6 and CFP-10 (T-SPOT.TB) as well as purified protein derivative (PPD) stimulation. All 146 participants were screened by the T-SPOT.TB assay at recruitment. T-SPOT.TB-positive rates in ATB and HC groups were 87.6% (78/89) and 21.1% (12/57), respectively. Of 38 ATB patients who were both TST and T-SPOT.TB tested, the positive rates were 73.7% (28/38) and 94.7% (36/38), respectively (P = 0.0215), and those in the HC group were 62.3% (33/53) and 18.9% (10/53), respectively (P < 0.0001). The T-SPOT.TB-positive rates declined during TB treatment and were 94.4% (51/54), 86.4% (19/22), and 61.5% (8/13) for ATB patients receiving 0- to 1-month, 1- to 3-month, and 3- to 6-month anti-TB treatment, respectively. The IGRA is a most promising test for both active TB and latent TB infection (LTBI) diagnosis due to the improvement of its specificity and convenience, especially in the Mycobacterium bovis BCG-vaccinated population. Furthermore, the T-SPOT.TB assay using ESAT-6 and CFP-10 in ATB patients during anti-TB treatment could serve as a potential predictor of therapeutic efficacy.

scholarly journals Use of Antigen-Specific Interleukin-2 To Differentiate between Cattle Vaccinated with Mycobacterium bovis BCG and Cattle Infected with M. bovis

2013 ◽  
Vol 21 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Shelley G. Rhodes ◽  
Lucy C. McKinna ◽  
Sabine Steinbach ◽  
Gilly S. Dean ◽  
Bernardo Villarreal-Ramos ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Whole Blood ◽  
Sandwich Elisa ◽  
Interleukin 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mycobacterial Antigen ◽  
Content Type ◽  
Purified Protein Derivative ◽  
Rapid Induction ◽  
Challenge Control

ABSTRACTWe describe here the application of a novel bovine interleukin-2 (IL-2) enzyme-linked immunosorbent assay (ELISA) for the measurement of antigen-specific IL-2 in cattle naturally infected withMycobacterium bovisand in cattle vaccinated withMycobacterium bovisBCG and then experimentally challenged with pathogenicM. bovis. Supernatants from whole-blood cultures stimulated with mycobacterial antigen (bovine purified protein derivative [PPDB] or the peptide cocktail ESAT6-CFP10) were assessed using a sandwich ELISA consisting of a new recombinant monoclonal fragment capture antibody and a commercially available polyclonal anti-bovine-IL-2. The production of IL-2 was compared to the production of gamma interferon (IFN-γ) in the same antigen-stimulated whole-blood supernatants. The data show that cattle infected withM. bovisproduced quantifiable levels of antigen-specific IL-2, while IL-2 levels in cattle vaccinated withM. bovisBCG did not. Furthermore, cattle vaccinated withM. bovisBCG and then challenged with pathogenicM. bovisdisplayed a more rapid induction of IL-2 but ultimately had lower levels of infection-induced IL-2 than did unvaccinated challenge control cattle. These data suggest that IL-2 responses are not detectable post-BCG vaccination and that these responses may require infection with virulentM. bovisto develop. This may be useful to differentiate infected cattle from uninfected or BCG-vaccinated cattle, although the overall sensitivity is relatively low, particularly in single intradermal comparative cervical tuberculin (SICCT)-negative infected animals. Furthermore, the strength of the IL-2 response may correlate with pathology, which poses interesting questions on the immunobiology of bovine tuberculosis in contrast to human tuberculosis, which is discussed.

Sign in / Sign up

Export Citation Format

Share Document